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The map label for this gene is hyfB [C]

Identifier: 148659845

GI number: 148659845

Start: 91758

End: 93680

Strand: Direct

Name: hyfB [C]

Synonym: MRA_0087

Alternate gene names: 148659845

Gene position: 91758-93680 (Clockwise)

Preceding gene: 148659844

Following gene: 148659846

Centisome position: 2.08

GC content: 71.19

Gene sequence:

>1923_bases
ATGACCGCTGCACCGACGGCCGGCGGGGTCGTCACTTCGGGCGTGGGCGTTGCCGGGGTCGGCGTGGGGTTGCTGGGCAT
GTTTGGACCGGTGCGTGTAGTGCACGTCGGTTGGCTGCTTCCGCTGTCCGGCGTGCACATCGAGCTCGACCGGTTGGGCG
GATTCTTCATGGCGCTCACGGGCGCGGTAGCGGCTCCGGTCGGTTGTTACCTGATCGGCTACGTGCGCCGTGAACACCTC
GGTCGGGTCCCGATGGCGGTGGTGCCGCTGTTCGTCGCGGCGATGCTGTTGGTGCCGGCCGCGGGCTCGGTGACGACGTT
TCTGCTGGCGTGGGAGCTGATGGCGATCGCGTCGCTGATCCTGGTGCTCTCCGAGCACGCCCGCCCGCAGGTCCGCTCGG
CGGGCCTGTGGTACGCCGTGATGACTCAGCTGGGATTCATCGCAATCCTGGTCGGGCTGGTGGTGTTGGCGGCGGCCGGG
GGTTCCGACCGGTTCGCCGGCCTCGGGGCAGTCTGCGACGGGGTCCGCGCCGCCGTATTTATGCTCACGCTGGTCGGGTT
TGGTTCGAAGGCGGGCCTGGTGCCACTGCACGCCTGGCTGCCGCGGGCCCACCCGGAGGCGCCGAGCCCGGTGTCGGCGT
TGATGAGCGCGGCGATGGTCAACCTGGGCATCTACGGCATCGTCCGTTTCGATCTGCAGCTGCTGGGGCCGGGCCCACGC
TGGTGGGGGCTTGCGCTGCTGGCCGTGGGCGGCACGTCCGCGCTGTATGGGGTGCTGCAGGCTTCGGTGGCCGCCGATCT
CAAACGGCTGCTGGCCTATTCGACGACCGAGAACATGGGCCTGATCACGCTGGCGCTCGGTGCGGCAACACTTTTCGCGG
ATACCGGAGCCTACGGGCCGGCGTCGATCGCCGCCGCCGCAGCGATGCTGCACATGATTGCGCACGCGGCGTTTAAGAGC
CTCGCCTTCATGGCGGCCGGATCTGTGCTGGCCGCGACCGGGCTGCGCGACCTGGACCTGCTCGGCGGGCTGGCCCGCCG
AATGCCGGCGACCACCGTCTTTTTCGGGGTGGCCGCACTGGGCGCATGTGGTCTGCCGTTGGGCGCCGGGTTTGTCAGTG
AGTGGCTGCTGGTCCAGTCGTTGATCCACGCTGCCCCCGGACACGACCCCATCGTGGCGCTGACGACACCGCTGGCGGTC
GGCGTGGTCGCACTGGCCACCGGTCTGAGCGTGGCGGCGATGACCAAGGCCTTCGGGATCGGGTTTCTCGCCCGTCCCCG
CTCCACCCAAGCCGAAGCGGCGCGTGAGGCGCCGGCCAGCATGCGCGCCGGCATGGCGATCGCGGCGGGCGCCTGCCTGG
TGCTGGCGGTGGCACCGCTGCTGGTCGCACCCATGGTGCGGCGGGCCGCCGCGACGCTGCCGGCCGCTCAGGCGGTCAAG
TTCACCGGTCTGGGCGCCGTGGTGCGGCTGCCCGCGATGTCCGGGTCGATCGCGCCCGGCGTGATCGCCGCCGCTGTGCT
CGCCGCGGCGTTGGCGGTAGCCGTCCTCGCGCGGTGGCGTTTCCGCCGGCGCCCGGCGCCGGCCAGGTTGCCGCTGTGGG
CTTGCGGCGCGGCCGATCTCACCGTGCGCATGCAATACACGGCCACGTCGTTCGCCGAGCCGCTGCAGCGGGTCTTCGGC
GACGTGCTGCGCCCGGACACCGACATCGAGGTCACCCACACCGCCGAGTCGCGCTATATGGCCGAGCGGATCACCTACCG
GACCGCGGTCGCCGACGCGATCGAACAGCGCCTCTATACTCCGGTGGTCGGGGCGGTGGCCGCCATGGCCGAGCTGCTGC
GCCGTGCCCACACCGGCAGCGTGCACCGCTACCTGGCCTACGGCGCGCTGGGCGTACTGATCGTGCTGGTGGTCGCGAGG
TGA

Upstream 100 bases:

>100_bases
TCGGTGCGGTCGGCGAGGTGGTACCCGTCGACGTCGAGATCGCCGGCTGCCCGCCGACACCCGCGGCCATCATGGCGGCG
CTGCGATCGGTGACCGGGAA

Downstream 100 bases:

>100_bases
ACGTGATGTCCTACCTAGCGGGCGCCGCGCAAATCGGCGGGGTCATGGTGGGTGCGCCGCTGGTCATCGGTATGACGCGG
CAGGTACGGGCACGCTGGGA

Product: putative NADH-ubiquinone oxidoreductase

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 640; Mature: 639

Protein sequence:

>640_residues
MTAAPTAGGVVTSGVGVAGVGVGLLGMFGPVRVVHVGWLLPLSGVHIELDRLGGFFMALTGAVAAPVGCYLIGYVRREHL
GRVPMAVVPLFVAAMLLVPAAGSVTTFLLAWELMAIASLILVLSEHARPQVRSAGLWYAVMTQLGFIAILVGLVVLAAAG
GSDRFAGLGAVCDGVRAAVFMLTLVGFGSKAGLVPLHAWLPRAHPEAPSPVSALMSAAMVNLGIYGIVRFDLQLLGPGPR
WWGLALLAVGGTSALYGVLQASVAADLKRLLAYSTTENMGLITLALGAATLFADTGAYGPASIAAAAAMLHMIAHAAFKS
LAFMAAGSVLAATGLRDLDLLGGLARRMPATTVFFGVAALGACGLPLGAGFVSEWLLVQSLIHAAPGHDPIVALTTPLAV
GVVALATGLSVAAMTKAFGIGFLARPRSTQAEAAREAPASMRAGMAIAAGACLVLAVAPLLVAPMVRRAAATLPAAQAVK
FTGLGAVVRLPAMSGSIAPGVIAAAVLAAALAVAVLARWRFRRRPAPARLPLWACGAADLTVRMQYTATSFAEPLQRVFG
DVLRPDTDIEVTHTAESRYMAERITYRTAVADAIEQRLYTPVVGAVAAMAELLRRAHTGSVHRYLAYGALGVLIVLVVAR

Sequences:

>Translated_640_residues
MTAAPTAGGVVTSGVGVAGVGVGLLGMFGPVRVVHVGWLLPLSGVHIELDRLGGFFMALTGAVAAPVGCYLIGYVRREHL
GRVPMAVVPLFVAAMLLVPAAGSVTTFLLAWELMAIASLILVLSEHARPQVRSAGLWYAVMTQLGFIAILVGLVVLAAAG
GSDRFAGLGAVCDGVRAAVFMLTLVGFGSKAGLVPLHAWLPRAHPEAPSPVSALMSAAMVNLGIYGIVRFDLQLLGPGPR
WWGLALLAVGGTSALYGVLQASVAADLKRLLAYSTTENMGLITLALGAATLFADTGAYGPASIAAAAAMLHMIAHAAFKS
LAFMAAGSVLAATGLRDLDLLGGLARRMPATTVFFGVAALGACGLPLGAGFVSEWLLVQSLIHAAPGHDPIVALTTPLAV
GVVALATGLSVAAMTKAFGIGFLARPRSTQAEAAREAPASMRAGMAIAAGACLVLAVAPLLVAPMVRRAAATLPAAQAVK
FTGLGAVVRLPAMSGSIAPGVIAAAVLAAALAVAVLARWRFRRRPAPARLPLWACGAADLTVRMQYTATSFAEPLQRVFG
DVLRPDTDIEVTHTAESRYMAERITYRTAVADAIEQRLYTPVVGAVAAMAELLRRAHTGSVHRYLAYGALGVLIVLVVAR
>Mature_639_residues
TAAPTAGGVVTSGVGVAGVGVGLLGMFGPVRVVHVGWLLPLSGVHIELDRLGGFFMALTGAVAAPVGCYLIGYVRREHLG
RVPMAVVPLFVAAMLLVPAAGSVTTFLLAWELMAIASLILVLSEHARPQVRSAGLWYAVMTQLGFIAILVGLVVLAAAGG
SDRFAGLGAVCDGVRAAVFMLTLVGFGSKAGLVPLHAWLPRAHPEAPSPVSALMSAAMVNLGIYGIVRFDLQLLGPGPRW
WGLALLAVGGTSALYGVLQASVAADLKRLLAYSTTENMGLITLALGAATLFADTGAYGPASIAAAAAMLHMIAHAAFKSL
AFMAAGSVLAATGLRDLDLLGGLARRMPATTVFFGVAALGACGLPLGAGFVSEWLLVQSLIHAAPGHDPIVALTTPLAVG
VVALATGLSVAAMTKAFGIGFLARPRSTQAEAAREAPASMRAGMAIAAGACLVLAVAPLLVAPMVRRAAATLPAAQAVKF
TGLGAVVRLPAMSGSIAPGVIAAAVLAAALAVAVLARWRFRRRPAPARLPLWACGAADLTVRMQYTATSFAEPLQRVFGD
VLRPDTDIEVTHTAESRYMAERITYRTAVADAIEQRLYTPVVGAVAAMAELLRRAHTGSVHRYLAYGALGVLIVLVVAR

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family

Homologues:

Organism=Homo sapiens, GI251831117, Length=466, Percent_Identity=24.4635193133047, Blast_Score=107, Evalue=5e-23,
Organism=Homo sapiens, GI251831116, Length=289, Percent_Identity=25.9515570934256, Blast_Score=73, Evalue=9e-13,
Organism=Escherichia coli, GI1788827, Length=636, Percent_Identity=30.9748427672956, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI2367154, Length=529, Percent_Identity=29.8676748582231, Blast_Score=167, Evalue=3e-42,
Organism=Escherichia coli, GI1788831, Length=358, Percent_Identity=33.7988826815642, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1788614, Length=343, Percent_Identity=30.6122448979592, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1788613, Length=219, Percent_Identity=28.7671232876712, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1788829, Length=335, Percent_Identity=27.4626865671642, Blast_Score=79, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y051_MYCTU (Q10880)

Other databases:

- EMBL:   BX842572
- EMBL:   AE000516
- PIR:   D70850
- RefSeq:   NP_214597.1
- RefSeq:   NP_334501.1
- ProteinModelPortal:   Q10880
- EnsemblBacteria:   EBMYCT00000002481
- EnsemblBacteria:   EBMYCT00000069409
- GeneID:   886965
- GeneID:   922874
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT0090
- KEGG:   mtu:Rv0083
- TIGR:   MT0090
- TubercuList:   Rv0083
- GeneTree:   EBGT00050000015714
- HOGENOM:   HBG643593
- OMA:   TAKNNRP
- ProtClustDB:   CLSK790248
- InterPro:   IPR001750
- InterPro:   IPR003918
- PRINTS:   PR01437

Pfam domain/function: PF00361 Oxidored_q1

EC number: 1.-.-.- [C]

Molecular weight: Translated: 65629; Mature: 65497

Theoretical pI: Translated: 10.37; Mature: 10.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1b82449c)-; HASH(0x1b388e84)-; HASH(0x1b388f44)-; HASH(0x1b054754)-; HASH(0x1b37ed2c)-; HASH(0x1b384d88)-; HASH(0x1b374944)-; HASH(0x1b37091c)-; HASH(0x1b38aea0)-; HASH(0x1b38f074)-; HASH(0x1b382e20)-; HASH(0x1b38f0bc)-; HASH(0x1b38cf3c)-; HASH(0x1b38b038)-;

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAAPTAGGVVTSGVGVAGVGVGLLGMFGPVRVVHVGWLLPLSGVHIELDRLGGFFMALT
CCCCCCCCCCHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCCCEEEHHHHCHHHHHHH
GAVAAPVGCYLIGYVRREHLGRVPMAVVPLFVAAMLLVPAAGSVTTFLLAWELMAIASLI
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LVLSEHARPQVRSAGLWYAVMTQLGFIAILVGLVVLAAAGGSDRFAGLGAVCDGVRAAVF
HHHHHCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
MLTLVGFGSKAGLVPLHAWLPRAHPEAPSPVSALMSAAMVNLGIYGIVRFDLQLLGPGPR
HHHHHCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEHHEECCCCH
WWGLALLAVGGTSALYGVLQASVAADLKRLLAYSTTENMGLITLALGAATLFADTGAYGP
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCH
ASIAAAAAMLHMIAHAAFKSLAFMAAGSVLAATGLRDLDLLGGLARRMPATTVFFGVAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHH
GACGLPLGAGFVSEWLLVQSLIHAAPGHDPIVALTTPLAVGVVALATGLSVAAMTKAFGI
HHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCC
GFLARPRSTQAEAAREAPASMRAGMAIAAGACLVLAVAPLLVAPMVRRAAATLPAAQAVK
CCCCCCCCCHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHE
FTGLGAVVRLPAMSGSIAPGVIAAAVLAAALAVAVLARWRFRRRPAPARLPLWACGAADL
ECCCCHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCE
TVRMQYTATSFAEPLQRVFGDVLRPDTDIEVTHTAESRYMAERITYRTAVADAIEQRLYT
EEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
PVVGAVAAMAELLRRAHTGSVHRYLAYGALGVLIVLVVAR
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure 
TAAPTAGGVVTSGVGVAGVGVGLLGMFGPVRVVHVGWLLPLSGVHIELDRLGGFFMALT
CCCCCCCCCHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCCCEEEHHHHCHHHHHHH
GAVAAPVGCYLIGYVRREHLGRVPMAVVPLFVAAMLLVPAAGSVTTFLLAWELMAIASLI
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LVLSEHARPQVRSAGLWYAVMTQLGFIAILVGLVVLAAAGGSDRFAGLGAVCDGVRAAVF
HHHHHCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
MLTLVGFGSKAGLVPLHAWLPRAHPEAPSPVSALMSAAMVNLGIYGIVRFDLQLLGPGPR
HHHHHCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEHHEECCCCH
WWGLALLAVGGTSALYGVLQASVAADLKRLLAYSTTENMGLITLALGAATLFADTGAYGP
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCH
ASIAAAAAMLHMIAHAAFKSLAFMAAGSVLAATGLRDLDLLGGLARRMPATTVFFGVAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHH
GACGLPLGAGFVSEWLLVQSLIHAAPGHDPIVALTTPLAVGVVALATGLSVAAMTKAFGI
HHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCC
GFLARPRSTQAEAAREAPASMRAGMAIAAGACLVLAVAPLLVAPMVRRAAATLPAAQAVK
CCCCCCCCCHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHE
FTGLGAVVRLPAMSGSIAPGVIAAAVLAAALAVAVLARWRFRRRPAPARLPLWACGAADL
ECCCCHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCE
TVRMQYTATSFAEPLQRVFGDVLRPDTDIEVTHTAESRYMAERITYRTAVADAIEQRLYT
EEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
PVVGAVAAMAELLRRAHTGSVHRYLAYGALGVLIVLVVAR
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036