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The map label for this gene is hycG [H]

Identifier: 148659844

GI number: 148659844

Start: 91282

End: 91761

Strand: Direct

Name: hycG [H]

Synonym: MRA_0086

Alternate gene names: 148659844

Gene position: 91282-91761 (Clockwise)

Preceding gene: 148659843

Following gene: 148659845

Centisome position: 2.07

GC content: 69.79

Gene sequence:

>480_bases
ATGGGTTGGGTTGCCAAGATTTTCCGTGTTGGCCGGGTGGTCGAGCCCGCGGCCCCCTTACCGGCGGCGATAGCCGAACC
ACCCGCCGGGGTACGGGGTTCGCTGCAGATCCGACATGTTGACGCGGGTTCGTGCAACGGGTGTGAGGTGGAGATTTCGG
GCGCCTTTGGCCCGGTGTATGACGCGGAGCGGTTCGGGGCGCGGCTGGTCGCCTCGCCCCGACACGCCGATGCGTTGTTG
GTGACCGGCGTGGTCACGCACAACATGGCCGGCCCACTGCGCAAGACCCTGGAGGCCACGCCGCGCCCGCGGGTGGTAAT
CGCGTGCGGGGATTGCGCGCTGAACCGGGGGGTGTTCGCCGACGCCTACGGCGTGGTCGGTGCGGTCGGCGAGGTGGTAC
CCGTCGACGTCGAGATCGCCGGCTGCCCGCCGACACCCGCGGCCATCATGGCGGCGCTGCGATCGGTGACCGGGAAATGA

Upstream 100 bases:

>100_bases
GAGCTGCTGGCGGTGGCACGCAAGGTGCTGGCCAGGGTGCTCAGCGACCGGGTGGCGGTGCTAGAGGACCTCCGCGCCGG
CGGCTCGGCCACGTAACGCC

Downstream 100 bases:

>100_bases
CCGCTGCACCGACGGCCGGCGGGGTCGTCACTTCGGGCGTGGGCGTTGCCGGGGTCGGCGTGGGGTTGCTGGGCATGTTT
GGACCGGTGCGTGTAGTGCA

Product: putative oxidoreductase

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 7; Hydrogenase-3 component G [H]

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MGWVAKIFRVGRVVEPAAPLPAAIAEPPAGVRGSLQIRHVDAGSCNGCEVEISGAFGPVYDAERFGARLVASPRHADALL
VTGVVTHNMAGPLRKTLEATPRPRVVIACGDCALNRGVFADAYGVVGAVGEVVPVDVEIAGCPPTPAAIMAALRSVTGK

Sequences:

>Translated_159_residues
MGWVAKIFRVGRVVEPAAPLPAAIAEPPAGVRGSLQIRHVDAGSCNGCEVEISGAFGPVYDAERFGARLVASPRHADALL
VTGVVTHNMAGPLRKTLEATPRPRVVIACGDCALNRGVFADAYGVVGAVGEVVPVDVEIAGCPPTPAAIMAALRSVTGK
>Mature_158_residues
GWVAKIFRVGRVVEPAAPLPAAIAEPPAGVRGSLQIRHVDAGSCNGCEVEISGAFGPVYDAERFGARLVASPRHADALLV
TGVVTHNMAGPLRKTLEATPRPRVVIACGDCALNRGVFADAYGVVGAVGEVVPVDVEIAGCPPTPAAIMAALRSVTGK

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=112, Percent_Identity=40.1785714285714, Blast_Score=94, Evalue=5e-20,
Organism=Escherichia coli, GI1789074, Length=140, Percent_Identity=45, Blast_Score=127, Evalue=5e-31,
Organism=Escherichia coli, GI1788834, Length=120, Percent_Identity=48.3333333333333, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1788624, Length=111, Percent_Identity=45.045045045045, Blast_Score=84, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17509685, Length=112, Percent_Identity=38.3928571428571, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24651058, Length=112, Percent_Identity=41.0714285714286, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI18859983, Length=112, Percent_Identity=40.1785714285714, Blast_Score=95, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24642371, Length=112, Percent_Identity=40.1785714285714, Blast_Score=95, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: NA

Molecular weight: Translated: 16260; Mature: 16129

Theoretical pI: Translated: 7.87; Mature: 7.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGWVAKIFRVGRVVEPAAPLPAAIAEPPAGVRGSLQIRHVDAGSCNGCEVEISGAFGPVY
CCHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCEEEEEECCCCCCCCEEEECCCCCCCC
DAERFGARLVASPRHADALLVTGVVTHNMAGPLRKTLEATPRPRVVIACGDCALNRGVFA
CHHHHCCEEECCCCCCCEEEEEHHHHHCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCH
DAYGVVGAVGEVVPVDVEIAGCPPTPAAIMAALRSVTGK
HHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
GWVAKIFRVGRVVEPAAPLPAAIAEPPAGVRGSLQIRHVDAGSCNGCEVEISGAFGPVY
CHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCEEEEEECCCCCCCCEEEECCCCCCCC
DAERFGARLVASPRHADALLVTGVVTHNMAGPLRKTLEATPRPRVVIACGDCALNRGVFA
CHHHHCCEEECCCCCCCEEEEEHHHHHCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCH
DAYGVVGAVGEVVPVDVEIAGCPPTPAAIMAALRSVTGK
HHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]