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The map label for this gene is dusA [H]

Identifier: 148653531

GI number: 148653531

Start: 2145115

End: 2146161

Strand: Direct

Name: dusA [H]

Synonym: PsycPRwf_1734

Alternate gene names: 148653531

Gene position: 2145115-2146161 (Clockwise)

Preceding gene: 148653530

Following gene: 148653538

Centisome position: 72.01

GC content: 49.19

Gene sequence:

>1047_bases
ATGACTGATACTGCCTCAGCTGTCACCGCAGCGCTTCATTCTGCCGATCTCATGCACAATAAGCGACTCTCAGTTGCCCC
TATGATCGACTGGACTACGACAGATTATCGTTTTTTTGCGCGTCTGTTTAATCCGCACACTTACTTATATACTGAGATGA
TTTCGACCAGTGCCATTTTACAAGGCAAAACCGATCGCTTACTGCGCTATGACACACTTGAGCATCCACTGGTATTACAG
CTAGGCGGTGCCGATATTAGCGAGATGACCCAGTGTGCTGTTGAAGCCCAAAAGCGCGGCTTTAATGAGGTTAATATTAA
TGTGGGCTGTCCCTCTGATAGAGTCCAGCACAACAAAATAGGTGCCTGCTTGATGGCTGAGCCTGATACCGTAGCCGCCT
TGGTGCGTAATATGCAAGCGCATGTTGATATTCCAGTGACTGTCAAGCATCGCATTGGCATAGATGAGCATGACAGTTAT
GAGTTTATGCAAGACTTCGTGAGTGCCAGTGCTGAAGCCGGTTGTCAGCGTTTTATCGTTCATGCCCGTATCGCTTGGCT
AAACGGACTAAGCCCTAAAGAAAACCGTGAGATTCCGCCACTGCGCTATGAGGATGTCTACCGCTTAAAGCAAGCGTTTC
CTGAGCTACATATTGAAATTAATGGCGGTATTGATAGCATTGAGCAGATCAAAGCCCATCTTCAGCAAGTCGACGGGGTC
ATGATTGGCCGTGCTGCCTACCACAATCCCTATATCTTGGCCGAAGCAATGTCGTTTTGGGGCGAGGCTGCGCCTAGTCG
CGCACAGATATTAACCAAATTGTACCCCTACTTAGAGCAGCAAGTGGCCAAGGGCGAGCCGCTACCTTCTTTGGCCCGTC
ACTTCTTGGGGTTGTTCCAAGGTCTGCCCGGTGCCCGCAAATGGCGACAAGCATTAAGCGGTAAGCAGCAAATGAGTGTC
GATGATATTCGCCGAGCAGCTGATGCGACCCTAGCCATCATTGAGCGTGCGTCTACTGATGATTTGCTTGATAATTCGCT
CAATTAA

Upstream 100 bases:

>100_bases
AGGTCATGATATAATGATCACTTGAGCCTTTTGCTGCTCAAGCTGTCTGCTACAAACCCTATCGTCTTCCCCTTTTTATT
CTAAGAGTACTTATCCTGTT

Downstream 100 bases:

>100_bases
GCCCTATCATGCCTGTTGGTGATGGCGCCCAATAATACAGACAAAAAAAGCTCAGTGCTGATGGCACTGAGCTTTTTTTG
TCTGTATTACTGGGTACTAA

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MTDTASAVTAALHSADLMHNKRLSVAPMIDWTTTDYRFFARLFNPHTYLYTEMISTSAILQGKTDRLLRYDTLEHPLVLQ
LGGADISEMTQCAVEAQKRGFNEVNINVGCPSDRVQHNKIGACLMAEPDTVAALVRNMQAHVDIPVTVKHRIGIDEHDSY
EFMQDFVSASAEAGCQRFIVHARIAWLNGLSPKENREIPPLRYEDVYRLKQAFPELHIEINGGIDSIEQIKAHLQQVDGV
MIGRAAYHNPYILAEAMSFWGEAAPSRAQILTKLYPYLEQQVAKGEPLPSLARHFLGLFQGLPGARKWRQALSGKQQMSV
DDIRRAADATLAIIERASTDDLLDNSLN

Sequences:

>Translated_348_residues
MTDTASAVTAALHSADLMHNKRLSVAPMIDWTTTDYRFFARLFNPHTYLYTEMISTSAILQGKTDRLLRYDTLEHPLVLQ
LGGADISEMTQCAVEAQKRGFNEVNINVGCPSDRVQHNKIGACLMAEPDTVAALVRNMQAHVDIPVTVKHRIGIDEHDSY
EFMQDFVSASAEAGCQRFIVHARIAWLNGLSPKENREIPPLRYEDVYRLKQAFPELHIEINGGIDSIEQIKAHLQQVDGV
MIGRAAYHNPYILAEAMSFWGEAAPSRAQILTKLYPYLEQQVAKGEPLPSLARHFLGLFQGLPGARKWRQALSGKQQMSV
DDIRRAADATLAIIERASTDDLLDNSLN
>Mature_347_residues
TDTASAVTAALHSADLMHNKRLSVAPMIDWTTTDYRFFARLFNPHTYLYTEMISTSAILQGKTDRLLRYDTLEHPLVLQL
GGADISEMTQCAVEAQKRGFNEVNINVGCPSDRVQHNKIGACLMAEPDTVAALVRNMQAHVDIPVTVKHRIGIDEHDSYE
FMQDFVSASAEAGCQRFIVHARIAWLNGLSPKENREIPPLRYEDVYRLKQAFPELHIEINGGIDSIEQIKAHLQQVDGVM
IGRAAYHNPYILAEAMSFWGEAAPSRAQILTKLYPYLEQQVAKGEPLPSLARHFLGLFQGLPGARKWRQALSGKQQMSVD
DIRRAADATLAIIERASTDDLLDNSLN

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=262, Percent_Identity=26.7175572519084, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI239788462, Length=262, Percent_Identity=26.7175572519084, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI145693211, Length=314, Percent_Identity=52.8662420382166, Blast_Score=333, Evalue=8e-93,
Organism=Escherichia coli, GI1789660, Length=253, Percent_Identity=27.2727272727273, Blast_Score=77, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323560, Length=237, Percent_Identity=25.7383966244726, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24580595, Length=303, Percent_Identity=26.7326732673267, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19920448, Length=303, Percent_Identity=26.7326732673267, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 38939; Mature: 38808

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTASAVTAALHSADLMHNKRLSVAPMIDWTTTDYRFFARLFNPHTYLYTEMISTSAIL
CCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHH
QGKTDRLLRYDTLEHPLVLQLGGADISEMTQCAVEAQKRGFNEVNINVGCPSDRVQHNKI
CCCCCHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCC
GACLMAEPDTVAALVRNMQAHVDIPVTVKHRIGIDEHDSYEFMQDFVSASAEAGCQRFIV
CEEEECCCHHHHHHHHHHHHHCCCCEEEHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
HARIAWLNGLSPKENREIPPLRYEDVYRLKQAFPELHIEINGGIDSIEQIKAHLQQVDGV
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCE
MIGRAAYHNPYILAEAMSFWGEAAPSRAQILTKLYPYLEQQVAKGEPLPSLARHFLGLFQ
EECHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
GLPGARKWRQALSGKQQMSVDDIRRAADATLAIIERASTDDLLDNSLN
CCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCC
>Mature Secondary Structure 
TDTASAVTAALHSADLMHNKRLSVAPMIDWTTTDYRFFARLFNPHTYLYTEMISTSAIL
CCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHH
QGKTDRLLRYDTLEHPLVLQLGGADISEMTQCAVEAQKRGFNEVNINVGCPSDRVQHNKI
CCCCCHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCC
GACLMAEPDTVAALVRNMQAHVDIPVTVKHRIGIDEHDSYEFMQDFVSASAEAGCQRFIV
CEEEECCCHHHHHHHHHHHHHCCCCEEEHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
HARIAWLNGLSPKENREIPPLRYEDVYRLKQAFPELHIEINGGIDSIEQIKAHLQQVDGV
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCE
MIGRAAYHNPYILAEAMSFWGEAAPSRAQILTKLYPYLEQQVAKGEPLPSLARHFLGLFQ
EECHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
GLPGARKWRQALSGKQQMSVDDIRRAADATLAIIERASTDDLLDNSLN
CCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]