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The map label for this gene is proP [H]

Identifier: 148653530

GI number: 148653530

Start: 2143671

End: 2144978

Strand: Direct

Name: proP [H]

Synonym: PsycPRwf_1733

Alternate gene names: 148653530

Gene position: 2143671-2144978 (Clockwise)

Preceding gene: 148653529

Following gene: 148653531

Centisome position: 71.96

GC content: 43.5

Gene sequence:

>1308_bases
ATGCCTTTTGTTGCCTTATCAGATTCCCAAAGCTCGGTGTCCCCAAAGCAATTAATGGTTATTTGCTTTGGTAATGCGGT
CGCCTATTTTGATTTTCTAATCTATCTTTTTATGGCAGATATCATTAGCGCCACTTTTTTTCCGGCCAATGATGATCCGT
TACTCGCCAAGCTACAAGCACTTACCATATTTATCGCCGGTTATTTAACTCGGCCCATTGGGGCGCTGCTGCTGGGCCGT
TATGCCGATGTCAAAGGCCGCAAACCTGCGCTTTTAATCAGCATCTCATTTATTGCTGCGACGGCACTTATTACCGCTTG
CCTGCCGACATACTCCCAAGTAGGCATTTTGGCACCCATCTTATTCTTTATCGCACGTATGTTTCAGGGCATGGCCTTTG
GTGCGCACACCCCCTTAGGCTGGGTGTATATTGCTGAGCATGTAGACAAGCGTAATCTGGCCACTTACTTAAGCTTTGTT
ACCGCAAGCTTTATGCTTGGTGAGCTGGGCTCAAACTTATTATTTGAAGTAATTACCAACACCCATACGCCCACTGAGCT
TATCGAAAGTGGTTGGCGTATTCCTTTTGTTTGGGGCGCTACGCTCAGTTTTGTGTCTTTGATGTTGTGGCACACTTTAC
CTGAGACGCCTATCTTTACCGCTCAAAAATGCAAACAGAAGTTTATGCCCAAAATATCTGAGATTGGGCCGTCATTTAAG
CGCTTTAATGCCATTTTCTTAGCCTTAGTACTGACCTTTATTATCAGTAGCTTGACAATGGTTGTGGCCTTGCTATTACC
GCAGCTAATTTCTATGAAGTTTAGTATTGATGAGTCTATGCTTAGCTTCTCAAATAATTTGGGCTTACTGTTTTTAATGA
TTGGTTGTATATTTTTTGGGTTAGTCGCAGATAAAAGCAGCACCGGTAAGGCTTTGATGATTGGCTCAATAGCCTTGACC
TTGCAGGCTTTAGCCTTTTATTACCACTTAGAAAACGGCGGTGGTGCGTTTATCTTATTTATGTACGCCATTTTAGGGTT
CTGTACGGGTATTATTAGCCTAGGCTCCGTCATCTTAGTACAGTTATTTCCTACTGAGGTTCGAGTCGCCGCCGTTTCGC
TCACTTATAACTTTATGTATGCGATAGTGGGGGTATCCCTACCCTTTGGTCTGGTCTATGCCACCAACTTGGTCAGCATC
TCGCCAGCGTTATACATTATCTTTGTGAGTATCGTCACCTTTATTATCGGGCTATATATTTACCGTTTGCCTAAGTTTAA
GTCGCTCGATGCATCTATTAAGCTATAG

Upstream 100 bases:

>100_bases
GGCTGTTGTAAGTTTTATACCCCTTATTTGATTAGCAGACGCGTCCTATCCGTTACGCTTAGGATGCTCATTTTACTTTT
AATCACTCGATACCTTTACT

Downstream 100 bases:

>100_bases
CTAATATAAACACAAAAGCCAAAAATCGGCTCAAGCAGGTCATGATATAATGATCACTTGAGCCTTTTGCTGCTCAAGCT
GTCTGCTACAAACCCTATCG

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MPFVALSDSQSSVSPKQLMVICFGNAVAYFDFLIYLFMADIISATFFPANDDPLLAKLQALTIFIAGYLTRPIGALLLGR
YADVKGRKPALLISISFIAATALITACLPTYSQVGILAPILFFIARMFQGMAFGAHTPLGWVYIAEHVDKRNLATYLSFV
TASFMLGELGSNLLFEVITNTHTPTELIESGWRIPFVWGATLSFVSLMLWHTLPETPIFTAQKCKQKFMPKISEIGPSFK
RFNAIFLALVLTFIISSLTMVVALLLPQLISMKFSIDESMLSFSNNLGLLFLMIGCIFFGLVADKSSTGKALMIGSIALT
LQALAFYYHLENGGGAFILFMYAILGFCTGIISLGSVILVQLFPTEVRVAAVSLTYNFMYAIVGVSLPFGLVYATNLVSI
SPALYIIFVSIVTFIIGLYIYRLPKFKSLDASIKL

Sequences:

>Translated_435_residues
MPFVALSDSQSSVSPKQLMVICFGNAVAYFDFLIYLFMADIISATFFPANDDPLLAKLQALTIFIAGYLTRPIGALLLGR
YADVKGRKPALLISISFIAATALITACLPTYSQVGILAPILFFIARMFQGMAFGAHTPLGWVYIAEHVDKRNLATYLSFV
TASFMLGELGSNLLFEVITNTHTPTELIESGWRIPFVWGATLSFVSLMLWHTLPETPIFTAQKCKQKFMPKISEIGPSFK
RFNAIFLALVLTFIISSLTMVVALLLPQLISMKFSIDESMLSFSNNLGLLFLMIGCIFFGLVADKSSTGKALMIGSIALT
LQALAFYYHLENGGGAFILFMYAILGFCTGIISLGSVILVQLFPTEVRVAAVSLTYNFMYAIVGVSLPFGLVYATNLVSI
SPALYIIFVSIVTFIIGLYIYRLPKFKSLDASIKL
>Mature_434_residues
PFVALSDSQSSVSPKQLMVICFGNAVAYFDFLIYLFMADIISATFFPANDDPLLAKLQALTIFIAGYLTRPIGALLLGRY
ADVKGRKPALLISISFIAATALITACLPTYSQVGILAPILFFIARMFQGMAFGAHTPLGWVYIAEHVDKRNLATYLSFVT
ASFMLGELGSNLLFEVITNTHTPTELIESGWRIPFVWGATLSFVSLMLWHTLPETPIFTAQKCKQKFMPKISEIGPSFKR
FNAIFLALVLTFIISSLTMVVALLLPQLISMKFSIDESMLSFSNNLGLLFLMIGCIFFGLVADKSSTGKALMIGSIALTL
QALAFYYHLENGGGAFILFMYAILGFCTGIISLGSVILVQLFPTEVRVAAVSLTYNFMYAIVGVSLPFGLVYATNLVSIS
PALYIIFVSIVTFIIGLYIYRLPKFKSLDASIKL

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=424, Percent_Identity=29.9528301886792, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1788942, Length=413, Percent_Identity=29.0556900726392, Blast_Score=128, Evalue=8e-31,
Organism=Escherichia coli, GI1789941, Length=396, Percent_Identity=28.5353535353535, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1788292, Length=383, Percent_Identity=27.154046997389, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI87082404, Length=408, Percent_Identity=23.5294117647059, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47721; Mature: 47589

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFVALSDSQSSVSPKQLMVICFGNAVAYFDFLIYLFMADIISATFFPANDDPLLAKLQA
CCEEEECCCCCCCCHHHEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
LTIFIAGYLTRPIGALLLGRYADVKGRKPALLISISFIAATALITACLPTYSQVGILAPI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
LFFIARMFQGMAFGAHTPLGWVYIAEHVDKRNLATYLSFVTASFMLGELGSNLLFEVITN
HHHHHHHHHCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
THTPTELIESGWRIPFVWGATLSFVSLMLWHTLPETPIFTAQKCKQKFMPKISEIGPSFK
CCCHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHHCCHHH
RFNAIFLALVLTFIISSLTMVVALLLPQLISMKFSIDESMLSFSNNLGLLFLMIGCIFFG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LVADKSSTGKALMIGSIALTLQALAFYYHLENGGGAFILFMYAILGFCTGIISLGSVILV
HHCCCCCCCCEEEHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QLFPTEVRVAAVSLTYNFMYAIVGVSLPFGLVYATNLVSISPALYIIFVSIVTFIIGLYI
HHCCCHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRLPKFKSLDASIKL
HHCCCCCCCCCCCCC
>Mature Secondary Structure 
PFVALSDSQSSVSPKQLMVICFGNAVAYFDFLIYLFMADIISATFFPANDDPLLAKLQA
CEEEECCCCCCCCHHHEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
LTIFIAGYLTRPIGALLLGRYADVKGRKPALLISISFIAATALITACLPTYSQVGILAPI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
LFFIARMFQGMAFGAHTPLGWVYIAEHVDKRNLATYLSFVTASFMLGELGSNLLFEVITN
HHHHHHHHHCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
THTPTELIESGWRIPFVWGATLSFVSLMLWHTLPETPIFTAQKCKQKFMPKISEIGPSFK
CCCHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHHCCHHH
RFNAIFLALVLTFIISSLTMVVALLLPQLISMKFSIDESMLSFSNNLGLLFLMIGCIFFG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LVADKSSTGKALMIGSIALTLQALAFYYHLENGGGAFILFMYAILGFCTGIISLGSVILV
HHCCCCCCCCEEEHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QLFPTEVRVAAVSLTYNFMYAIVGVSLPFGLVYATNLVSISPALYIIFVSIVTFIIGLYI
HHCCCHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRLPKFKSLDASIKL
HHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]