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The map label for this gene is insB [H]

Identifier: 148652511

GI number: 148652511

Start: 841813

End: 842514

Strand: Direct

Name: insB [H]

Synonym: PsycPRwf_0700

Alternate gene names: 148652511

Gene position: 841813-842514 (Clockwise)

Preceding gene: 148652509

Following gene: 148652512

Centisome position: 28.26

GC content: 39.74

Gene sequence:

>702_bases
ATGCACATCACACTATACATCAAATGCCCAGCCTGTCTAAGTGACAATATAAAGAAAAATGGCTTCAAAAGCTATGGTAA
ACAAAACTATAAATGCAAAGACTGCAAACGGCAGTTTATTGGAGACCATGCCTTAACGTACCAAGGATGTCACTCCCAAA
AAGATAGCAAAATACGATATCTTATGGTTCGAGGCAGTGGCATAAAAGACATCGCTTGCGTTGAGAGAATCAGCAAAGGC
AAAGTACTAGCCACCCTAAAAAAGTGTCACTACCCAATAACCCCCAAGCAAAGGCAATACGACTGTCTTGAAATTGATGA
GCTTTGGACATTCGTAGGTAAAAAAACCAACAAACAATGGCTAATTTATGCCTATCACCGTGACACAGGTGAAATTGTTG
CCTATGTGTGGGGAAAACGAGACCTTAACACTGTCAAAAAGCTTAAAGCTAAGCTGAAAGCCCTAGGCGTAAGTTGTGCT
AGAATCGCTAGTGATACCTGGGATAGCTTTGTTACTGGTTTTAAGGGCTTTACCCAAGTCATTGGCAAGTTTTTCACGGT
CGGCATTGAAGGTAATAATTGCACTATCAGACATCGAGTAAGGCGAGCATTTAGGCGAAGTTGTAACTTTTCTAAAAAAC
TGGAAAATCACTTTAAAGCCTTTGATTTAGCTTTCTTTTACATCAATCATGGGTATGTTTAA

Upstream 100 bases:

>100_bases
TTTTAAAAGTTGTTTTATTCATAAAGGTATCTGAATCCGGGTGGTGGTTCAAAAAGTAGGCTGGGAAAAAAACAGGCTGA
GAAATTGATAAAATAGTGAA

Downstream 100 bases:

>100_bases
TTTAGCCTACTTTTTGAACCACCACCCGCTTATGTATTAAAAACACGACCCTTTTAGTCGATTCACAAAGTAGGACAGAA
ACGTTATTTATGCGCTATGG

Product: IS1 transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MHITLYIKCPACLSDNIKKNGFKSYGKQNYKCKDCKRQFIGDHALTYQGCHSQKDSKIRYLMVRGSGIKDIACVERISKG
KVLATLKKCHYPITPKQRQYDCLEIDELWTFVGKKTNKQWLIYAYHRDTGEIVAYVWGKRDLNTVKKLKAKLKALGVSCA
RIASDTWDSFVTGFKGFTQVIGKFFTVGIEGNNCTIRHRVRRAFRRSCNFSKKLENHFKAFDLAFFYINHGYV

Sequences:

>Translated_233_residues
MHITLYIKCPACLSDNIKKNGFKSYGKQNYKCKDCKRQFIGDHALTYQGCHSQKDSKIRYLMVRGSGIKDIACVERISKG
KVLATLKKCHYPITPKQRQYDCLEIDELWTFVGKKTNKQWLIYAYHRDTGEIVAYVWGKRDLNTVKKLKAKLKALGVSCA
RIASDTWDSFVTGFKGFTQVIGKFFTVGIEGNNCTIRHRVRRAFRRSCNFSKKLENHFKAFDLAFFYINHGYV
>Mature_233_residues
MHITLYIKCPACLSDNIKKNGFKSYGKQNYKCKDCKRQFIGDHALTYQGCHSQKDSKIRYLMVRGSGIKDIACVERISKG
KVLATLKKCHYPITPKQRQYDCLEIDELWTFVGKKTNKQWLIYAYHRDTGEIVAYVWGKRDLNTVKKLKAKLKALGVSCA
RIASDTWDSFVTGFKGFTQVIGKFFTVGIEGNNCTIRHRVRRAFRRSCNFSKKLENHFKAFDLAFFYINHGYV

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family [H]

Homologues:

Organism=Escherichia coli, GI1786470, Length=120, Percent_Identity=36.6666666666667, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1786459, Length=120, Percent_Identity=36.6666666666667, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1787223, Length=129, Percent_Identity=35.6589147286822, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1789853, Length=129, Percent_Identity=34.8837209302326, Blast_Score=80, Evalue=8e-17,
Organism=Escherichia coli, GI1788203, Length=129, Percent_Identity=34.8837209302326, Blast_Score=80, Evalue=8e-17,
Organism=Escherichia coli, GI1786203, Length=129, Percent_Identity=34.8837209302326, Blast_Score=80, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005063 [H]

Pfam domain/function: PF03400 Transposase_27 [H]

EC number: NA

Molecular weight: Translated: 26997; Mature: 26997

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
4.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHITLYIKCPACLSDNIKKNGFKSYGKQNYKCKDCKRQFIGDHALTYQGCHSQKDSKIRY
CEEEEEEECCHHHCCCCCCCCHHHHCCCCCCHHHHHHHHHCCCCEEECCCCCCCCCCEEE
LMVRGSGIKDIACVERISKGKVLATLKKCHYPITPKQRQYDCLEIDELWTFVGKKTNKQW
EEEECCCCCHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEE
LIYAYHRDTGEIVAYVWGKRDLNTVKKLKAKLKALGVSCARIASDTWDSFVTGFKGFTQV
EEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
IGKFFTVGIEGNNCTIRHRVRRAFRRSCNFSKKLENHFKAFDLAFFYINHGYV
HHHHEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEEEECCCC
>Mature Secondary Structure
MHITLYIKCPACLSDNIKKNGFKSYGKQNYKCKDCKRQFIGDHALTYQGCHSQKDSKIRY
CEEEEEEECCHHHCCCCCCCCHHHHCCCCCCHHHHHHHHHCCCCEEECCCCCCCCCCEEE
LMVRGSGIKDIACVERISKGKVLATLKKCHYPITPKQRQYDCLEIDELWTFVGKKTNKQW
EEEECCCCCHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEE
LIYAYHRDTGEIVAYVWGKRDLNTVKKLKAKLKALGVSCARIASDTWDSFVTGFKGFTQV
EEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
IGKFFTVGIEGNNCTIRHRVRRAFRRSCNFSKKLENHFKAFDLAFFYINHGYV
HHHHEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6265806 [H]