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The map label for this gene is nusA [H]

Identifier: 148651972

GI number: 148651972

Start: 184592

End: 186079

Strand: Direct

Name: nusA [H]

Synonym: PsycPRwf_0156

Alternate gene names: 148651972

Gene position: 184592-186079 (Clockwise)

Preceding gene: 148651971

Following gene: 148651973

Centisome position: 6.2

GC content: 43.88

Gene sequence:

>1488_bases
ATGAGTCGAGAAATTTTAACGGTAGTAGAAACCGTCAGTAACGAGAAAGGCCTAAATCCAGAAGACATCTTTGAGGCAAT
TGAAGAGGCTTTGGTGGTTTCTACCAAAAAAAGAGTTTATACCGAGCAGCCAGAAGTGGCAGTAAGGGTTGAAATTGACC
GTGATACCGGTGATTATGATACCTATCGCTATTGGACTGTGGTTGCCGACGAAGACCACGAAATGCCAGCATGTCAGTTA
GCCATCAGTGATTTAGATGAAAACGAATGGTCTATTGGCGACATTAAAGAAGAGCAAATCGAATCTATCGAATTTGGACG
TATTGCCGCCACTCAGGCCAAGCAAGTGATTATCCAAAAAATTCGTGAAGCAGAGCGTGCATTAACTGCCGATGCCTTCG
AGCCAAGAATTGGTGAGATGATGTATGGTGAAGTTAAAAAACAAACGCGTGATGGCTATATTATTGATTTAGGGGATAAT
GCTGAAGGCTATTTATCTCGTGATCAAATGCTGCCTCGTGAACAATTACGTGTAAAGTCTCGTATTAATGCTATCCTATA
CCATGTGAACCGTGACAACCGCGGCGCACAGTTATTGTTATCGCGCACCAGCCCAGATATGTTGGTGGCGTTAATGAACA
AAGAAGTGCCTGAGATTAACGAACAAATCATTGAAATCCGTGATGTGGCTCGTCAGCCTGGGGTTCGTGCTAAAATTTCA
GTAAAAACCAATGACCATCGTATTGATCCTGTGGGCGCGTGTATTGGTATGCGTGGTACCCGTATCCAAGCGGTACAATC
TGAGCTTGATGGCGAGCGTATTGATGTGATCGTGTGGTCTGATGACCCCGCGCAATATATCATCAGTGCACTTGAGCCTG
CAGATGTTAGCAGTATTATTTTAGATGAAGATGCCAAGACCGCAGACATCATCTTTACCGCCAACGATCAGTTAGCGCGC
GCCATTGGTGCCCAAGGCCAGAACGTACGTTTGGCTTCTGAGCTAACCGGTTATAAGTTAAACATGATGCTTGAGGCGGA
ATACCTTGAACGTCAACAAAACGAAACAAAAGCCTATCTTGAGCTGTTCTACAATCGTCTAGAAGTAGATGAAGACTTAG
CTCAAGCGCTAGTAGATGTAGGCTTTACCAGTATTGAAGAAGTGGCTTATGTGCCTGTCGAAACCTTCTATGACATCGAA
GGTCTTGACGACGACGCAATTGAAATGATTCAAGAGCGTGCAAAAGAAGTAGTCATTGCTGATGAACTGGTTAAACAGCA
AAATATGAAAGAGCCAAGCAAAGAGTTGCTCACAATGGACGGCATGACGACCGACTGGGCGTACAAGCTGGCTCAACGTG
ACATCATCACGTTAGATGATTTAGCAGAACAGGCAGTATTTGATCTTGAAGACATTGAAGGTCTTGATGAAAAAACTGCT
GGTGAGTTGATCATGAAAGCACGTGAATCTTGGTTTAATGAAGAATAA

Upstream 100 bases:

>100_bases
GAATGAGATTAAACATCGCAGCTTTTAGGCCTAAGATAAAAAATAAATTTTAATAATTTTTTGCTACTTTAAATGACAAG
TAGGCGAGGATAGGTGTGTA

Downstream 100 bases:

>100_bases
TAAATAGGTGACTATAAATGGCAGATAAGACCGTCAAAGAATTAGCAGACATGGTAAGCAAGACAGTCAGTGCAGTACAA
AAGCAACTGACTGATGCTGG

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MSREILTVVETVSNEKGLNPEDIFEAIEEALVVSTKKRVYTEQPEVAVRVEIDRDTGDYDTYRYWTVVADEDHEMPACQL
AISDLDENEWSIGDIKEEQIESIEFGRIAATQAKQVIIQKIREAERALTADAFEPRIGEMMYGEVKKQTRDGYIIDLGDN
AEGYLSRDQMLPREQLRVKSRINAILYHVNRDNRGAQLLLSRTSPDMLVALMNKEVPEINEQIIEIRDVARQPGVRAKIS
VKTNDHRIDPVGACIGMRGTRIQAVQSELDGERIDVIVWSDDPAQYIISALEPADVSSIILDEDAKTADIIFTANDQLAR
AIGAQGQNVRLASELTGYKLNMMLEAEYLERQQNETKAYLELFYNRLEVDEDLAQALVDVGFTSIEEVAYVPVETFYDIE
GLDDDAIEMIQERAKEVVIADELVKQQNMKEPSKELLTMDGMTTDWAYKLAQRDIITLDDLAEQAVFDLEDIEGLDEKTA
GELIMKARESWFNEE

Sequences:

>Translated_495_residues
MSREILTVVETVSNEKGLNPEDIFEAIEEALVVSTKKRVYTEQPEVAVRVEIDRDTGDYDTYRYWTVVADEDHEMPACQL
AISDLDENEWSIGDIKEEQIESIEFGRIAATQAKQVIIQKIREAERALTADAFEPRIGEMMYGEVKKQTRDGYIIDLGDN
AEGYLSRDQMLPREQLRVKSRINAILYHVNRDNRGAQLLLSRTSPDMLVALMNKEVPEINEQIIEIRDVARQPGVRAKIS
VKTNDHRIDPVGACIGMRGTRIQAVQSELDGERIDVIVWSDDPAQYIISALEPADVSSIILDEDAKTADIIFTANDQLAR
AIGAQGQNVRLASELTGYKLNMMLEAEYLERQQNETKAYLELFYNRLEVDEDLAQALVDVGFTSIEEVAYVPVETFYDIE
GLDDDAIEMIQERAKEVVIADELVKQQNMKEPSKELLTMDGMTTDWAYKLAQRDIITLDDLAEQAVFDLEDIEGLDEKTA
GELIMKARESWFNEE
>Mature_494_residues
SREILTVVETVSNEKGLNPEDIFEAIEEALVVSTKKRVYTEQPEVAVRVEIDRDTGDYDTYRYWTVVADEDHEMPACQLA
ISDLDENEWSIGDIKEEQIESIEFGRIAATQAKQVIIQKIREAERALTADAFEPRIGEMMYGEVKKQTRDGYIIDLGDNA
EGYLSRDQMLPREQLRVKSRINAILYHVNRDNRGAQLLLSRTSPDMLVALMNKEVPEINEQIIEIRDVARQPGVRAKISV
KTNDHRIDPVGACIGMRGTRIQAVQSELDGERIDVIVWSDDPAQYIISALEPADVSSIILDEDAKTADIIFTANDQLARA
IGAQGQNVRLASELTGYKLNMMLEAEYLERQQNETKAYLELFYNRLEVDEDLAQALVDVGFTSIEEVAYVPVETFYDIEG
LDDDAIEMIQERAKEVVIADELVKQQNMKEPSKELLTMDGMTTDWAYKLAQRDIITLDDLAEQAVFDLEDIEGLDEKTAG
ELIMKARESWFNEE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=498, Percent_Identity=49.3975903614458, Blast_Score=483, Evalue=1e-137,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR003583
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 56216; Mature: 56085

Theoretical pI: Translated: 4.09; Mature: 4.09

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSREILTVVETVSNEKGLNPEDIFEAIEEALVVSTKKRVYTEQPEVAVRVEIDRDTGDYD
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCC
TYRYWTVVADEDHEMPACQLAISDLDENEWSIGDIKEEQIESIEFGRIAATQAKQVIIQK
CEEEEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHH
IREAERALTADAFEPRIGEMMYGEVKKQTRDGYIIDLGDNAEGYLSRDQMLPREQLRVKS
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHCCCHHHHHHHH
RINAILYHVNRDNRGAQLLLSRTSPDMLVALMNKEVPEINEQIIEIRDVARQPGVRAKIS
HHCEEEEEECCCCCCCEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEE
VKTNDHRIDPVGACIGMRGTRIQAVQSELDGERIDVIVWSDDPAQYIISALEPADVSSII
EECCCCCCCHHHHHHCCCCCHHHHHHHHCCCCEEEEEEECCCHHHHHHHHCCCCCCCEEE
LDEDAKTADIIFTANDQLARAIGAQGQNVRLASELTGYKLNMMLEAEYLERQQNETKAYL
ECCCCCEEEEEEECCHHHHHHHCCCCCCEEEHHHCCCEEEEEEEEHHHHHHHHHHHHHHH
ELFYNRLEVDEDLAQALVDVGFTSIEEVAYVPVETFYDIEGLDDDAIEMIQERAKEVVIA
HHHHHHHCCCHHHHHHHHHCCCHHHHHHEECCHHHEECCCCCCHHHHHHHHHHHHHHHHH
DELVKQQNMKEPSKELLTMDGMTTDWAYKLAQRDIITLDDLAEQAVFDLEDIEGLDEKTA
HHHHHHHCCCCCHHHHEECCCCCHHHHHHHHHCCCEEHHHHHHHHHHCHHHHCCCCHHHH
GELIMKARESWFNEE
HHHHHHHHHHHCCCC
>Mature Secondary Structure 
SREILTVVETVSNEKGLNPEDIFEAIEEALVVSTKKRVYTEQPEVAVRVEIDRDTGDYD
CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCC
TYRYWTVVADEDHEMPACQLAISDLDENEWSIGDIKEEQIESIEFGRIAATQAKQVIIQK
CEEEEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHH
IREAERALTADAFEPRIGEMMYGEVKKQTRDGYIIDLGDNAEGYLSRDQMLPREQLRVKS
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHCCCHHHHHHHH
RINAILYHVNRDNRGAQLLLSRTSPDMLVALMNKEVPEINEQIIEIRDVARQPGVRAKIS
HHCEEEEEECCCCCCCEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEE
VKTNDHRIDPVGACIGMRGTRIQAVQSELDGERIDVIVWSDDPAQYIISALEPADVSSII
EECCCCCCCHHHHHHCCCCCHHHHHHHHCCCCEEEEEEECCCHHHHHHHHCCCCCCCEEE
LDEDAKTADIIFTANDQLARAIGAQGQNVRLASELTGYKLNMMLEAEYLERQQNETKAYL
ECCCCCEEEEEEECCHHHHHHHCCCCCCEEEHHHCCCEEEEEEEEHHHHHHHHHHHHHHH
ELFYNRLEVDEDLAQALVDVGFTSIEEVAYVPVETFYDIEGLDDDAIEMIQERAKEVVIA
HHHHHHHCCCHHHHHHHHHCCCHHHHHHEECCHHHEECCCCCCHHHHHHHHHHHHHHHHH
DELVKQQNMKEPSKELLTMDGMTTDWAYKLAQRDIITLDDLAEQAVFDLEDIEGLDEKTA
HHHHHHHCCCCCHHHHEECCCCCHHHHHHHHHCCCEEHHHHHHHHHHCHHHHCCCCHHHH
GELIMKARESWFNEE
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12704232 [H]