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The map label for this gene is tpiA

Identifier: 148651969

GI number: 148651969

Start: 181557

End: 182354

Strand: Direct

Name: tpiA

Synonym: PsycPRwf_0152

Alternate gene names: 148651969

Gene position: 181557-182354 (Clockwise)

Preceding gene: 148651964

Following gene: 148651970

Centisome position: 6.09

GC content: 48.62

Gene sequence:

>798_bases
ATGAAGTCTTGGGTTATCGGAAACTGGAAGCTAAATCCTACGTCGCTTGAGCAAGTACAGCACTTGGTGACAGACTTAGT
AGAAGGCGTTGACGCTCAAACCCGCAGCATGGATAATTGCCATCTTATGTTGGCCCCAAGCTTTTTACATTTAAGCCCAG
TGCAGCAGGCACTTAACGCCAACTCATCTACTCTTAAGCTGGCTGCGCAAGATGTGAGCGCACTCAGTGCTGAATCAGGG
GCTTATACTGGTGATGTTTCGGCTAAGCAGCTAAAAGATATGGGCGTACAGTGGGTGATTGTCGGTCACTCTGAGCGCCG
TCAGTACTATAAAGAAAGCAACGCCGTACTGCTAAAAAAGCTGCTTAATAGCGCTGAGCAAGGCTTGGGCGTGATTTTGT
GCATTGGTGAGTCAGAGAGCGACTTTGAGGCGGGTCACACTGAGCAAGTGTTGGATGAGGAGTTACAAGTTATTGCTGAC
TTTTTACAGCAATTAAAGCCTGAGCAGGCGGGATATGCCGAGCAAAACCTGATTATCGCTTATGAGCCGGTGTGGGCCAT
TGGTACCGGTAAGGTGCCAAGTGTAGAGCAGGTGACGCAAATACATCGCTTTATCCGTCAGCAGTTGCATTCATTTGATA
ATAAGCTTGATACCACCCCAGTGATTTATGGTGGCAGCGTTAAGCCTGAGAATGCTGCAGATTTTGCTGCCTCAGACCAG
ATCAATGGGGTGTTGGTTGGCGGCGCCGCCCTGCAGGCAGACAGCTTTCTTGCGATTGCTAAATGCTTTAATAACTAG

Upstream 100 bases:

>100_bases
CCTAAACAATGCGCTGTCACAATGTTGTTTGTTATTTTGGTTTTATTTATTCGGGGCTTAATAGGCCCTTTTGATTAATT
GGTTTTTAGGTGAGCGTGTT

Downstream 100 bases:

>100_bases
ATAAAATAAGCGGCGCTAGGCTTGATAGCTTAGCGCTCTGCCCTGATAAACGGTGACATGAACGTTGTCATAATAGATAG
TTGTTGTGGTAAAACTGGGG

Product: Triose-phosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MKSWVIGNWKLNPTSLEQVQHLVTDLVEGVDAQTRSMDNCHLMLAPSFLHLSPVQQALNANSSTLKLAAQDVSALSAESG
AYTGDVSAKQLKDMGVQWVIVGHSERRQYYKESNAVLLKKLLNSAEQGLGVILCIGESESDFEAGHTEQVLDEELQVIAD
FLQQLKPEQAGYAEQNLIIAYEPVWAIGTGKVPSVEQVTQIHRFIRQQLHSFDNKLDTTPVIYGGSVKPENAADFAASDQ
INGVLVGGAALQADSFLAIAKCFNN

Sequences:

>Translated_265_residues
MKSWVIGNWKLNPTSLEQVQHLVTDLVEGVDAQTRSMDNCHLMLAPSFLHLSPVQQALNANSSTLKLAAQDVSALSAESG
AYTGDVSAKQLKDMGVQWVIVGHSERRQYYKESNAVLLKKLLNSAEQGLGVILCIGESESDFEAGHTEQVLDEELQVIAD
FLQQLKPEQAGYAEQNLIIAYEPVWAIGTGKVPSVEQVTQIHRFIRQQLHSFDNKLDTTPVIYGGSVKPENAADFAASDQ
INGVLVGGAALQADSFLAIAKCFNN
>Mature_265_residues
MKSWVIGNWKLNPTSLEQVQHLVTDLVEGVDAQTRSMDNCHLMLAPSFLHLSPVQQALNANSSTLKLAAQDVSALSAESG
AYTGDVSAKQLKDMGVQWVIVGHSERRQYYKESNAVLLKKLLNSAEQGLGVILCIGESESDFEAGHTEQVLDEELQVIAD
FLQQLKPEQAGYAEQNLIIAYEPVWAIGTGKVPSVEQVTQIHRFIRQQLHSFDNKLDTTPVIYGGSVKPENAADFAASDQ
INGVLVGGAALQADSFLAIAKCFNN

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=255, Percent_Identity=34.9019607843137, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI226529917, Length=255, Percent_Identity=34.9019607843137, Blast_Score=150, Evalue=1e-36,
Organism=Escherichia coli, GI1790353, Length=258, Percent_Identity=41.8604651162791, Blast_Score=189, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17536593, Length=255, Percent_Identity=40, Blast_Score=169, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6320255, Length=254, Percent_Identity=35.8267716535433, Blast_Score=136, Evalue=3e-33,
Organism=Drosophila melanogaster, GI28572008, Length=255, Percent_Identity=36.078431372549, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI28572006, Length=255, Percent_Identity=36.078431372549, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI28572004, Length=258, Percent_Identity=36.046511627907, Blast_Score=145, Evalue=4e-35,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_PSYWF (A5WBS1)

Other databases:

- EMBL:   CP000713
- RefSeq:   YP_001279062.1
- STRING:   A5WBS1
- GeneID:   5205917
- GenomeReviews:   CP000713_GR
- KEGG:   prw:PsycPRwf_0152
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- ProtClustDB:   PRK00042
- BioCyc:   PSP56811:PSYCPRWF_0152-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 28865; Mature: 28865

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 103-103 ACT_SITE 182-182 BINDING 8-8 BINDING 10-10

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSWVIGNWKLNPTSLEQVQHLVTDLVEGVDAQTRSMDNCHLMLAPSFLHLSPVQQALNA
CCCEEEECEEECHHHHHHHHHHHHHHHHCCCHHHCCCCCCEEEECCCHHHHHHHHHHHCC
NSSTLKLAAQDVSALSAESGAYTGDVSAKQLKDMGVQWVIVGHSERRQYYKESNAVLLKK
CCCEEEEHHHHHHHHHHCCCCCCCCCCHHHHHHCCEEEEEECCHHHHHHHHHCCHHHHHH
LLNSAEQGLGVILCIGESESDFEAGHTEQVLDEELQVIADFLQQLKPEQAGYAEQNLIIA
HHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
YEPVWAIGTGKVPSVEQVTQIHRFIRQQLHSFDNKLDTTPVIYGGSVKPENAADFAASDQ
ECCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCC
INGVLVGGAALQADSFLAIAKCFNN
CCEEEEECHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKSWVIGNWKLNPTSLEQVQHLVTDLVEGVDAQTRSMDNCHLMLAPSFLHLSPVQQALNA
CCCEEEECEEECHHHHHHHHHHHHHHHHCCCHHHCCCCCCEEEECCCHHHHHHHHHHHCC
NSSTLKLAAQDVSALSAESGAYTGDVSAKQLKDMGVQWVIVGHSERRQYYKESNAVLLKK
CCCEEEEHHHHHHHHHHCCCCCCCCCCHHHHHHCCEEEEEECCHHHHHHHHHCCHHHHHH
LLNSAEQGLGVILCIGESESDFEAGHTEQVLDEELQVIADFLQQLKPEQAGYAEQNLIIA
HHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
YEPVWAIGTGKVPSVEQVTQIHRFIRQQLHSFDNKLDTTPVIYGGSVKPENAADFAASDQ
ECCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCC
INGVLVGGAALQADSFLAIAKCFNN
CCEEEEECHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA