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The map label for this gene is rlmB [H]

Identifier: 148651964

GI number: 148651964

Start: 175574

End: 176359

Strand: Direct

Name: rlmB [H]

Synonym: PsycPRwf_0147

Alternate gene names: 148651964

Gene position: 175574-176359 (Clockwise)

Preceding gene: 148651963

Following gene: 148651969

Centisome position: 5.89

GC content: 52.42

Gene sequence:

>786_bases
ATGGCAAAGCCCGTCCATTTTTATGGCATTCACGCAATTCACGCTTTGTTGTCGCACCGTCCTACCGACGCCATGGCTTT
ATTTATTCAGCAAGGCCGTGCTCACAAAGACAGCAGCACTCAGGACGAAGCCGACTCTACCTTTGAAGAGATTTTACAAT
TGGCTGAGCAGATGGGGGTGAGCATCCAGGAGGCCAGCCGTGATAAGCTGACCGCATTGTGCGGTAGTCCGCAGCACCAA
GGTGTGGTACTCAATGCCCGTCCGTTAGCCATGGCCGATGAAGGTGAGATTGCAAGTCTTGCTCAATCAGATGATGCCCT
ATTTTTGGTGTTAGACCAGATTACGGATGCGCATAACCTAGGTGCCTGCTTGCGTACGGCAGCGGCGATGGGTGTTGATG
CGGTGATTTGCCCTAAGCACCATTCAGCGAGCCTCACACCAACTGTGGCCAAAGTATCGGTAGGTGCCATGGAAATCATG
CCAGTCATCGCTGTGACTAATCTGGCACGCTGTATTGAGAACTTAAAGCAAGCAGGGGTGTTTGTTTATGGTACGGCGCT
TGACGCCACCGCTAAGGCTCTGCCAGATGTGGACCTAAAGGGTAAGGTGGCCATCATTATGGGTTCAGAGGGTGAGGGCA
TGCGCCGCTTGACCACCGAGCGCTGTGATGAGCTGGTGTACATTCCCATGACTGGTAATAAGCATGGCTCACTACAAAGC
TTGAATGTCAGTGTGGCCACAGGCATGGTCTTGTATGAAGTGAGCCGTCAGCGTCATCAGCCTTAA

Upstream 100 bases:

>100_bases
TCATCGCGTTGTGTCTTATAATAAGCGAGAAACGCAGTCATCAAGTTGCGCCGTATGGCTGCCCTCACCGTTAAAATCCA
TCGTTTAAAAAGGTTATTTT

Downstream 100 bases:

>100_bases
TATCAGTCTGATTAAGACAGACAACAGCTTAAGAGGCAAAAAGGGCGCGCTTAGCATAAGCGCGCCCTTTGTGTGTCAAG
GCACGGGGCTTTATTGCACG

Product: RNA methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MAKPVHFYGIHAIHALLSHRPTDAMALFIQQGRAHKDSSTQDEADSTFEEILQLAEQMGVSIQEASRDKLTALCGSPQHQ
GVVLNARPLAMADEGEIASLAQSDDALFLVLDQITDAHNLGACLRTAAAMGVDAVICPKHHSASLTPTVAKVSVGAMEIM
PVIAVTNLARCIENLKQAGVFVYGTALDATAKALPDVDLKGKVAIIMGSEGEGMRRLTTERCDELVYIPMTGNKHGSLQS
LNVSVATGMVLYEVSRQRHQP

Sequences:

>Translated_261_residues
MAKPVHFYGIHAIHALLSHRPTDAMALFIQQGRAHKDSSTQDEADSTFEEILQLAEQMGVSIQEASRDKLTALCGSPQHQ
GVVLNARPLAMADEGEIASLAQSDDALFLVLDQITDAHNLGACLRTAAAMGVDAVICPKHHSASLTPTVAKVSVGAMEIM
PVIAVTNLARCIENLKQAGVFVYGTALDATAKALPDVDLKGKVAIIMGSEGEGMRRLTTERCDELVYIPMTGNKHGSLQS
LNVSVATGMVLYEVSRQRHQP
>Mature_260_residues
AKPVHFYGIHAIHALLSHRPTDAMALFIQQGRAHKDSSTQDEADSTFEEILQLAEQMGVSIQEASRDKLTALCGSPQHQG
VVLNARPLAMADEGEIASLAQSDDALFLVLDQITDAHNLGACLRTAAAMGVDAVICPKHHSASLTPTVAKVSVGAMEIMP
VIAVTNLARCIENLKQAGVFVYGTALDATAKALPDVDLKGKVAIIMGSEGEGMRRLTTERCDELVYIPMTGNKHGSLQSL
NVSVATGMVLYEVSRQRHQP

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=221, Percent_Identity=33.9366515837104, Blast_Score=117, Evalue=1e-26,
Organism=Escherichia coli, GI1790623, Length=253, Percent_Identity=44.2687747035573, Blast_Score=194, Evalue=5e-51,
Organism=Escherichia coli, GI1788935, Length=260, Percent_Identity=28.0769230769231, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1790083, Length=162, Percent_Identity=31.4814814814815, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17555908, Length=260, Percent_Identity=25.7692307692308, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324775, Length=253, Percent_Identity=27.2727272727273, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27906; Mature: 27775

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKPVHFYGIHAIHALLSHRPTDAMALFIQQGRAHKDSSTQDEADSTFEEILQLAEQMGV
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
SIQEASRDKLTALCGSPQHQGVVLNARPLAMADEGEIASLAQSDDALFLVLDQITDAHNL
CHHHHHHHHHHHHCCCCCCCCEEEECCCCEECCCCCHHHHHCCCCEEEEEEEHHCCCHHH
GACLRTAAAMGVDAVICPKHHSASLTPTVAKVSVGAMEIMPVIAVTNLARCIENLKQAGV
HHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCE
FVYGTALDATAKALPDVDLKGKVAIIMGSEGEGMRRLTTERCDELVYIPMTGNKHGSLQS
EEEEECHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCCCEEE
LNVSVATGMVLYEVSRQRHQP
EEEHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKPVHFYGIHAIHALLSHRPTDAMALFIQQGRAHKDSSTQDEADSTFEEILQLAEQMGV
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
SIQEASRDKLTALCGSPQHQGVVLNARPLAMADEGEIASLAQSDDALFLVLDQITDAHNL
CHHHHHHHHHHHHCCCCCCCCEEEECCCCEECCCCCHHHHHCCCCEEEEEEEHHCCCHHH
GACLRTAAAMGVDAVICPKHHSASLTPTVAKVSVGAMEIMPVIAVTNLARCIENLKQAGV
HHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCE
FVYGTALDATAKALPDVDLKGKVAIIMGSEGEGMRRLTTERCDELVYIPMTGNKHGSLQS
EEEEECHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCCCEEE
LNVSVATGMVLYEVSRQRHQP
EEEHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA