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The map label for this gene is fixL [H]

Identifier: 148550730

GI number: 148550730

Start: 194232

End: 195734

Strand: Reverse

Name: fixL [H]

Synonym: Swit_5294

Alternate gene names: 148550730

Gene position: 195734-194232 (Counterclockwise)

Preceding gene: 148550737

Following gene: 148550729

Centisome position: 63.09

GC content: 67.47

Gene sequence:

>1503_bases
GTGGCCCGCGGCAATGGTGCAAGCCGGCTCCTGCCCCGGCTCGCCCTGCCCGCTGCCCTCGGCGTCGCGCTGCTCGCGAT
CGCCGGTGGCCTCGCCGTACGGCTCCTGTTTGCCGATCAGCTCGGCGCGCGCGCGACCTTCATCTTCTTCGTCCCGGGCG
TGGTCGCCGCAAGCGCCCTGTCAGGCCTTCGCGTCGGCGCGATGGCTTCGCTGGCCGGCGCAGCCGCCGGCCTGTGGTGC
GACCACCTGACAGGCCCGATCGAGGAAGGCAGCGTAATTGCTGCGGTGGCTTTCGTAGCGATCGGGCTCGCCATCGCGCT
AGGGGGCGAGTGGTTTCAGCGAGCCCGTCTTGCTTCGGACGAGGCCGCCGCCGGCCTCGCCAGCCGAGAGGCGCATCTGC
GCTCGATCCTCGACACCGTCCCCGACGCCATGGTGGTGATCGACGCTGCGGGGCTCATTCGCGACTTCAGCCCAGCGGCG
GAACGGATGTTCGGCTGGAAGGCGCAGGAGATAGCGGGCCGCAACGTGCGGCTGCTGATGCCCGAGCCCTACCGCGCAGC
CCATGATGGCTATCTGGATCGCTATTACCAGACGGGAGAGCGCCGCATCATCGGCAAGGGCCGCGTCGTCGTAGGCGAAC
GCCGCGACGGATCGACCTTTCCGCTCGAACTCGCCGTCGGCGAAGTCCAGGTCAACGGACAGCGCTTTTTCACCGGCTTC
ATCCGCGATCTCACCGAGCGCCAACATGCCGAGGCGAGGCTGCAGGAACTGCAGAGCGAACTCGTTCACGTCTCGCGGCT
GACCGCCCTGGGTGAAATGGCCTCGGCCCTCGCCCATGAACTGAACCAGCCCCTCTCCGCCATCGCCAACTATCTCAAGG
GCAGCAAGATGCTGCTCGAACGCGAGGAAGATCGCAACGACCGGGTTATCGATGCGGTCGGCCGCGCTGCCGCCGAGGCG
CTGCGCGCCGGGAAAATCATCCGCCGGCTGCGCGATTTCGTCGCGCGCGGCGAAACCGAACGAGCGGTCGAAAGCCTGCC
GAAGCTTGTCGAGGAGGCGAGCGCGCTGGCGCTCGTTGGCGCCAAGGAGCATGGGATACGCGTCCAGCATGACTTCAGCC
CTGAGATCGACCTCGTGCTCGCGGACAAGGTGCAAATCCAGCAGGTGGTGCTCAACCTCGTTCGCAATGCCATCGACGCC
ATGGCCGGGAATGGATCGGGGCCACGTGCGCTGACCATCGCCATCGCGCCGGCCGACACCGACATGGCGCGGGTGACGGT
CGCCGATACCGGCCCGGGCATCGCGGCCGATGTGGCAGACCGGCTGTTTCAACCATTCATCACCACGAAACGCACCGGCA
TGGGTGTAGGCCTCTCGATCTCGCGCACGATCGTCGAGGCCCATGGCGGGCGCATTTGGGCCGAATCTCATGAAGGGGGC
GGGGCCGTGTTCGGCTTCACGCTGCCCAAGGCGGACAAGGAAGGGCCAGGCGATGGCGAGTGA

Upstream 100 bases:

>100_bases
TTGACGGGGCGCCGCTGATGGGCCGTATGTAGACGGGGAAAGGAAGGACGAAAATTTGGATGATGTCCCTACCGCAGTGC
CCCTAAGGGACTTACCGGAG

Downstream 100 bases:

>100_bases
TCCGATTGTTTATGTGATCGATGACGACGATAGCGTCCGGCATTCGCTGGAATTTCTCCTCGATGTGGCGGGGATACGGG
TGCGCTCCTATGCTTCGGCC

Product: PAS/PAC sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MARGNGASRLLPRLALPAALGVALLAIAGGLAVRLLFADQLGARATFIFFVPGVVAASALSGLRVGAMASLAGAAAGLWC
DHLTGPIEEGSVIAAVAFVAIGLAIALGGEWFQRARLASDEAAAGLASREAHLRSILDTVPDAMVVIDAAGLIRDFSPAA
ERMFGWKAQEIAGRNVRLLMPEPYRAAHDGYLDRYYQTGERRIIGKGRVVVGERRDGSTFPLELAVGEVQVNGQRFFTGF
IRDLTERQHAEARLQELQSELVHVSRLTALGEMASALAHELNQPLSAIANYLKGSKMLLEREEDRNDRVIDAVGRAAAEA
LRAGKIIRRLRDFVARGETERAVESLPKLVEEASALALVGAKEHGIRVQHDFSPEIDLVLADKVQIQQVVLNLVRNAIDA
MAGNGSGPRALTIAIAPADTDMARVTVADTGPGIAADVADRLFQPFITTKRTGMGVGLSISRTIVEAHGGRIWAESHEGG
GAVFGFTLPKADKEGPGDGE

Sequences:

>Translated_500_residues
MARGNGASRLLPRLALPAALGVALLAIAGGLAVRLLFADQLGARATFIFFVPGVVAASALSGLRVGAMASLAGAAAGLWC
DHLTGPIEEGSVIAAVAFVAIGLAIALGGEWFQRARLASDEAAAGLASREAHLRSILDTVPDAMVVIDAAGLIRDFSPAA
ERMFGWKAQEIAGRNVRLLMPEPYRAAHDGYLDRYYQTGERRIIGKGRVVVGERRDGSTFPLELAVGEVQVNGQRFFTGF
IRDLTERQHAEARLQELQSELVHVSRLTALGEMASALAHELNQPLSAIANYLKGSKMLLEREEDRNDRVIDAVGRAAAEA
LRAGKIIRRLRDFVARGETERAVESLPKLVEEASALALVGAKEHGIRVQHDFSPEIDLVLADKVQIQQVVLNLVRNAIDA
MAGNGSGPRALTIAIAPADTDMARVTVADTGPGIAADVADRLFQPFITTKRTGMGVGLSISRTIVEAHGGRIWAESHEGG
GAVFGFTLPKADKEGPGDGE
>Mature_499_residues
ARGNGASRLLPRLALPAALGVALLAIAGGLAVRLLFADQLGARATFIFFVPGVVAASALSGLRVGAMASLAGAAAGLWCD
HLTGPIEEGSVIAAVAFVAIGLAIALGGEWFQRARLASDEAAAGLASREAHLRSILDTVPDAMVVIDAAGLIRDFSPAAE
RMFGWKAQEIAGRNVRLLMPEPYRAAHDGYLDRYYQTGERRIIGKGRVVVGERRDGSTFPLELAVGEVQVNGQRFFTGFI
RDLTERQHAEARLQELQSELVHVSRLTALGEMASALAHELNQPLSAIANYLKGSKMLLEREEDRNDRVIDAVGRAAAEAL
RAGKIIRRLRDFVARGETERAVESLPKLVEEASALALVGAKEHGIRVQHDFSPEIDLVLADKVQIQQVVLNLVRNAIDAM
AGNGSGPRALTIAIAPADTDMARVTVADTGPGIAADVADRLFQPFITTKRTGMGVGLSISRTIVEAHGGRIWAESHEGGG
AVFGFTLPKADKEGPGDGE

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=364, Percent_Identity=28.021978021978, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1790436, Length=288, Percent_Identity=32.2916666666667, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1790300, Length=362, Percent_Identity=26.2430939226519, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI48994928, Length=265, Percent_Identity=25.6603773584906, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI145693157, Length=270, Percent_Identity=28.8888888888889, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI87081921, Length=122, Percent_Identity=27.8688524590164, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1789149, Length=245, Percent_Identity=27.3469387755102, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1788713, Length=233, Percent_Identity=25.3218884120172, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 53038; Mature: 52907

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARGNGASRLLPRLALPAALGVALLAIAGGLAVRLLFADQLGARATFIFFVPGVVAASAL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHH
SGLRVGAMASLAGAAAGLWCDHLTGPIEEGSVIAAVAFVAIGLAIALGGEWFQRARLASD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHCCCC
EAAAGLASREAHLRSILDTVPDAMVVIDAAGLIRDFSPAAERMFGWKAQEIAGRNVRLLM
HHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHCCCHHHHHHHCCCHHHHCCCCEEEEC
PEPYRAAHDGYLDRYYQTGERRIIGKGRVVVGERRDGSTFPLELAVGEVQVNGQRFFTGF
CCCCCHHHCHHHHHHHHCCCEEEEECCCEEEEECCCCCCCEEEEEEEEEEECCHHHHHHH
IRDLTERQHAEARLQELQSELVHVSRLTALGEMASALAHELNQPLSAIANYLKGSKMLLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHH
REEDRNDRVIDAVGRAAAEALRAGKIIRRLRDFVARGETERAVESLPKLVEEASALALVG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEEE
AKEHGIRVQHDFSPEIDLVLADKVQIQQVVLNLVRNAIDAMAGNGSGPRALTIAIAPADT
CCCCCEEEEECCCCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCC
DMARVTVADTGPGIAADVADRLFQPFITTKRTGMGVGLSISRTIVEAHGGRIWAESHEGG
CCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCC
GAVFGFTLPKADKEGPGDGE
CEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
ARGNGASRLLPRLALPAALGVALLAIAGGLAVRLLFADQLGARATFIFFVPGVVAASAL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHH
SGLRVGAMASLAGAAAGLWCDHLTGPIEEGSVIAAVAFVAIGLAIALGGEWFQRARLASD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHCCCC
EAAAGLASREAHLRSILDTVPDAMVVIDAAGLIRDFSPAAERMFGWKAQEIAGRNVRLLM
HHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHCCCHHHHHHHCCCHHHHCCCCEEEEC
PEPYRAAHDGYLDRYYQTGERRIIGKGRVVVGERRDGSTFPLELAVGEVQVNGQRFFTGF
CCCCCHHHCHHHHHHHHCCCEEEEECCCEEEEECCCCCCCEEEEEEEEEEECCHHHHHHH
IRDLTERQHAEARLQELQSELVHVSRLTALGEMASALAHELNQPLSAIANYLKGSKMLLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHH
REEDRNDRVIDAVGRAAAEALRAGKIIRRLRDFVARGETERAVESLPKLVEEASALALVG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEEE
AKEHGIRVQHDFSPEIDLVLADKVQIQQVVLNLVRNAIDAMAGNGSGPRALTIAIAPADT
CCCCCEEEEECCCCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCC
DMARVTVADTGPGIAADVADRLFQPFITTKRTGMGVGLSISRTIVEAHGGRIWAESHEGG
CCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCC
GAVFGFTLPKADKEGPGDGE
CEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2046550; 1766374 [H]