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The map label for this gene is fixJ [H]

Identifier: 148550729

GI number: 148550729

Start: 193631

End: 194242

Strand: Reverse

Name: fixJ [H]

Synonym: Swit_5293

Alternate gene names: 148550729

Gene position: 194242-193631 (Counterclockwise)

Preceding gene: 148550730

Following gene: 148550727

Centisome position: 62.61

GC content: 64.38

Gene sequence:

>612_bases
ATGGCGAGTGATCCGATTGTTTATGTGATCGATGACGACGATAGCGTCCGGCATTCGCTGGAATTTCTCCTCGATGTGGC
GGGGATACGGGTGCGCTCCTATGCTTCGGCCGACGCCTTTCTCAAATCATCGCCGCCTCTCGCCGGCGCCTGCCTCGTGA
CCGACGTGCGCATGCCGGGGATGACCGGCATCGAGCTGGCCGAAAAGCTGCAGCACGGGGGCGCAGGAGTCCCTGTCATC
GTCGTGACCGGGCATGCCGACGTCCCGCTGGCGATCCAGGCGATGAAAGCCGGCGTGGCGGACTTCATCGAAAAGCCGTT
CGATGACGAGGTCATGCTGAGCGCGATCCGCAACGCGCTGGCTCGCACCGCGGGAGATGCCGCCTCCGCCACGGAAAGGC
AGGCCATCCTCGACCGCATCGCCACGCTCAGCCCGCGCGAGCGCGAGGTCATGGACGGCCTCGTCGCGGGCCAGGCGAAC
AAGGCGATCGCCTATGACCTGGAGATCAGCGCGAGGACGGTCGAGGTCTACCGCGCCAATGTCATGATGAAAATGCAGGC
AAAGACCCTGTCCGACCTCGTGCGGATGGTCACGATCGCGCGGCTATCCTGA

Upstream 100 bases:

>100_bases
GTCGAGGCCCATGGCGGGCGCATTTGGGCCGAATCTCATGAAGGGGGCGGGGCCGTGTTCGGCTTCACGCTGCCCAAGGC
GGACAAGGAAGGGCCAGGCG

Downstream 100 bases:

>100_bases
GAGGCACCAAGGATCGTCCTTGATGATCGGCAGAAGGGCTCGCCCGGCCCTCCGCGACCGTCGGACAAGAGAAGGGCCGA
TCTCAACGAGACAGCCCCTC

Product: two component LuxR family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 202

Protein sequence:

>203_residues
MASDPIVYVIDDDDSVRHSLEFLLDVAGIRVRSYASADAFLKSSPPLAGACLVTDVRMPGMTGIELAEKLQHGGAGVPVI
VVTGHADVPLAIQAMKAGVADFIEKPFDDEVMLSAIRNALARTAGDAASATERQAILDRIATLSPREREVMDGLVAGQAN
KAIAYDLEISARTVEVYRANVMMKMQAKTLSDLVRMVTIARLS

Sequences:

>Translated_203_residues
MASDPIVYVIDDDDSVRHSLEFLLDVAGIRVRSYASADAFLKSSPPLAGACLVTDVRMPGMTGIELAEKLQHGGAGVPVI
VVTGHADVPLAIQAMKAGVADFIEKPFDDEVMLSAIRNALARTAGDAASATERQAILDRIATLSPREREVMDGLVAGQAN
KAIAYDLEISARTVEVYRANVMMKMQAKTLSDLVRMVTIARLS
>Mature_202_residues
ASDPIVYVIDDDDSVRHSLEFLLDVAGIRVRSYASADAFLKSSPPLAGACLVTDVRMPGMTGIELAEKLQHGGAGVPVIV
VTGHADVPLAIQAMKAGVADFIEKPFDDEVMLSAIRNALARTAGDAASATERQAILDRIATLSPREREVMDGLVAGQANK
AIAYDLEISARTVEVYRANVMMKMQAKTLSDLVRMVTIARLS

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=103, Percent_Identity=35.9223300970874, Blast_Score=77, Evalue=6e-16,
Organism=Escherichia coli, GI1788222, Length=190, Percent_Identity=30.5263157894737, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1789809, Length=134, Percent_Identity=29.1044776119403, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1790102, Length=197, Percent_Identity=30.4568527918782, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788905, Length=88, Percent_Identity=36.3636363636364, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1788550, Length=108, Percent_Identity=29.6296296296296, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 21697; Mature: 21566

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASDPIVYVIDDDDSVRHSLEFLLDVAGIRVRSYASADAFLKSSPPLAGACLVTDVRMPG
CCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCEEEEEECCCCC
MTGIELAEKLQHGGAGVPVIVVTGHADVPLAIQAMKAGVADFIEKPFDDEVMLSAIRNAL
CCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
ARTAGDAASATERQAILDRIATLSPREREVMDGLVAGQANKAIAYDLEISARTVEVYRAN
HHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEEEECHHEEEEHHHH
VMMKMQAKTLSDLVRMVTIARLS
HHEEHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ASDPIVYVIDDDDSVRHSLEFLLDVAGIRVRSYASADAFLKSSPPLAGACLVTDVRMPG
CCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCEEEEEECCCCC
MTGIELAEKLQHGGAGVPVIVVTGHADVPLAIQAMKAGVADFIEKPFDDEVMLSAIRNAL
CCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
ARTAGDAASATERQAILDRIATLSPREREVMDGLVAGQANKAIAYDLEISARTVEVYRAN
HHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEEEECHHEEEEHHHH
VMMKMQAKTLSDLVRMVTIARLS
HHEEHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2046550 [H]