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The map label for this gene is xthA [H]

Identifier: 148550719

GI number: 148550719

Start: 183159

End: 183968

Strand: Direct

Name: xthA [H]

Synonym: Swit_5283

Alternate gene names: 148550719

Gene position: 183159-183968 (Clockwise)

Preceding gene: 148550718

Following gene: 148550720

Centisome position: 59.04

GC content: 63.7

Gene sequence:

>810_bases
GTGAAGATCGCCACCTATAATGTGAACGGCGTCAATGGCCGGCTGCCGGTGCTGCTGCGCTGGCTTGAGGAAGCGCAGCC
TGATGTGGTTGTGCTCCAGGAATTGAAGGCGCCGCAGGAGAAGTTTCCGGAAAGTGCGATCCGCGATCTTGGCTATGATG
TCATCTGGCACGGACAGAAGTCGTGGAACGGCGTTGCGCTGCTCAGCCGCGTCGGCGAAATCCACGAGACGCGGCGGGGG
CTTCCGGGCGATCCCGACCCGGGCCAGAGCCGCTATATCGAGGCGGCGGTCAACGGCATCCTGATCGGCGGGCTTTACCT
GCCAAACGGTAATCCGCGGCCTGGCCCCAAGTTCGACTACAAGCTGCGCTGGTTCGACCGGCTGATCGAGCATGCGGCGG
GGTTACTGGAGAGCGGACTGCCGGTGATGCTGGCGGGCGACTTCAATGTCATGCCGACCGATCTCGACGTCTACAAGCCG
GAGCGCTGGCTCGACGACGCGCTATTCGCACCCGAAGTAAGGGCGGCCTATTTCCGGCTGCTGGATCAGGGCTGGACCGA
CGCGCTGCGCACGTTGCACCCGGGCGAGGTCATCTACACCTTCTGGGACTATTTCCGGAACGCCTATGGCCGCAATGCCG
GCCTGCGCATCGATCACCTGCTGCTCAGCCCCGCGCTTGCCGACCGGCTGCTCGATGCCCAGGTCGACCGCCATGTCCGG
GGCTGGGAAAAGACCAGCGACCATGCCCCGGTCTGGATCGAACTGGCCGACAAGCCAAAGCGCAAGCGAGCCCCCAAGGC
GAAAATATGA

Upstream 100 bases:

>100_bases
CCTCGCACTGGACCATTCGCGATGGCGCCGAACTGGTGAAGCGGGCCGGCTCCAAGGCGCTCAGCGGTTGGGGCCGCGCC
GACCAGACCCTGCCTGACCT

Downstream 100 bases:

>100_bases
TGGAGATCGAAAGCTTCCTGCTCGGCGAGAATGACTGGGTGCCGAACAATGCGCGGTTGCCGGTCGTCATCTATCGCAGC
GCGCTGGACGGCGAGGGCGA

Product: exodeoxyribonuclease III Xth

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MKIATYNVNGVNGRLPVLLRWLEEAQPDVVVLQELKAPQEKFPESAIRDLGYDVIWHGQKSWNGVALLSRVGEIHETRRG
LPGDPDPGQSRYIEAAVNGILIGGLYLPNGNPRPGPKFDYKLRWFDRLIEHAAGLLESGLPVMLAGDFNVMPTDLDVYKP
ERWLDDALFAPEVRAAYFRLLDQGWTDALRTLHPGEVIYTFWDYFRNAYGRNAGLRIDHLLLSPALADRLLDAQVDRHVR
GWEKTSDHAPVWIELADKPKRKRAPKAKI

Sequences:

>Translated_269_residues
MKIATYNVNGVNGRLPVLLRWLEEAQPDVVVLQELKAPQEKFPESAIRDLGYDVIWHGQKSWNGVALLSRVGEIHETRRG
LPGDPDPGQSRYIEAAVNGILIGGLYLPNGNPRPGPKFDYKLRWFDRLIEHAAGLLESGLPVMLAGDFNVMPTDLDVYKP
ERWLDDALFAPEVRAAYFRLLDQGWTDALRTLHPGEVIYTFWDYFRNAYGRNAGLRIDHLLLSPALADRLLDAQVDRHVR
GWEKTSDHAPVWIELADKPKRKRAPKAKI
>Mature_269_residues
MKIATYNVNGVNGRLPVLLRWLEEAQPDVVVLQELKAPQEKFPESAIRDLGYDVIWHGQKSWNGVALLSRVGEIHETRRG
LPGDPDPGQSRYIEAAVNGILIGGLYLPNGNPRPGPKFDYKLRWFDRLIEHAAGLLESGLPVMLAGDFNVMPTDLDVYKP
ERWLDDALFAPEVRAAYFRLLDQGWTDALRTLHPGEVIYTFWDYFRNAYGRNAGLRIDHLLLSPALADRLLDAQVDRHVR
GWEKTSDHAPVWIELADKPKRKRAPKAKI

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=263, Percent_Identity=30.4182509505703, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI18375503, Length=263, Percent_Identity=30.4182509505703, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI18375501, Length=263, Percent_Identity=30.4182509505703, Blast_Score=108, Evalue=4e-24,
Organism=Escherichia coli, GI1788046, Length=265, Percent_Identity=38.8679245283019, Blast_Score=160, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI71989536, Length=262, Percent_Identity=26.3358778625954, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI221330655, Length=256, Percent_Identity=30.46875, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI17136678, Length=256, Percent_Identity=30.46875, Blast_Score=106, Evalue=2e-23,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30561; Mature: 30561

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIATYNVNGVNGRLPVLLRWLEEAQPDVVVLQELKAPQEKFPESAIRDLGYDVIWHGQK
CEEEEEECCCCCCHHHHHHHHHHHCCCCEEEHHHHCCCHHHCHHHHHHHCCCCEEECCCC
SWNGVALLSRVGEIHETRRGLPGDPDPGQSRYIEAAVNGILIGGLYLPNGNPRPGPKFDY
CCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCEEEEEEEECCCCCCCCCCCCCH
KLRWFDRLIEHAAGLLESGLPVMLAGDFNVMPTDLDVYKPERWLDDALFAPEVRAAYFRL
HHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHH
LDQGWTDALRTLHPGEVIYTFWDYFRNAYGRNAGLRIDHLLLSPALADRLLDAQVDRHVR
HHCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
GWEKTSDHAPVWIELADKPKRKRAPKAKI
CCCCCCCCCCEEEEECCCCHHHCCCCCCC
>Mature Secondary Structure
MKIATYNVNGVNGRLPVLLRWLEEAQPDVVVLQELKAPQEKFPESAIRDLGYDVIWHGQK
CEEEEEECCCCCCHHHHHHHHHHHCCCCEEEHHHHCCCHHHCHHHHHHHCCCCEEECCCC
SWNGVALLSRVGEIHETRRGLPGDPDPGQSRYIEAAVNGILIGGLYLPNGNPRPGPKFDY
CCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCEEEEEEEECCCCCCCCCCCCCH
KLRWFDRLIEHAAGLLESGLPVMLAGDFNVMPTDLDVYKPERWLDDALFAPEVRAAYFRL
HHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHH
LDQGWTDALRTLHPGEVIYTFWDYFRNAYGRNAGLRIDHLLLSPALADRLLDAQVDRHVR
HHCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
GWEKTSDHAPVWIELADKPKRKRAPKAKI
CCCCCCCCCCEEEEECCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]