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The map label for this gene is hmp [H]

Identifier: 148550642

GI number: 148550642

Start: 93931

End: 94380

Strand: Reverse

Name: hmp [H]

Synonym: Swit_5203

Alternate gene names: 148550642

Gene position: 94380-93931 (Counterclockwise)

Preceding gene: 148550644

Following gene: 148550641

Centisome position: 30.42

GC content: 62.44

Gene sequence:

>450_bases
GTGACCAAAGCATTGTCTCCCCAAACCATGACGTTGGTCAAAGCGACGGCGCCAGCCCTTCAACAGCATGGCGTGGACAT
CACCACGCGCATGTATCAGCGGCTATTTGTCGATCCCGAGATCAAGGCGCTGTTCGACATGGCCGCGCAGGAGTCGGGCG
CGCAACCCAAGCGCCTTGCGGCGGCGATCCTGGCGTTCGCCCAGAATGTGGACAAGCTCGACGTCCTGAAACCGGCGATC
GAGCGGATCGCCGGGCGGCACGTCGATACCCATATCAAACCAGAGCATTATCCTGCGGTCGCCAATGCCTTGCTGCCGGC
CATTCGCGACATCCTGGGCGAGGCCGCGACGGACGAAATCCTGGCGGCATGGGGCGAAGCCTATTGGTTCCTCGCCGACA
TTCTGATCGCGCGCGAGGCCGAGCTTTATGACGAGGCCCAGGCCGCATGA

Upstream 100 bases:

>100_bases
GGTCGATCTAGTCGAAATAACAGAGCCGAGTTTGCGCCAGCGCAAACCGGCTCGCACCGACAGCCTCTACAAGCAAACTG
TTATCATGAAGAGGATCGTC

Downstream 100 bases:

>100_bases
CGGGGCCGGCGCCCTACAGCTCGTCGCCGGTGTTCGATCAGGACACATTGCCGGCGGCGCTGCGCGCGCGCCATGACACC
AAGGCGGGGGTGTGGGGACT

Product: nitric oxide dioxygenase

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD [H]

Number of amino acids: Translated: 149; Mature: 148

Protein sequence:

>149_residues
MTKALSPQTMTLVKATAPALQQHGVDITTRMYQRLFVDPEIKALFDMAAQESGAQPKRLAAAILAFAQNVDKLDVLKPAI
ERIAGRHVDTHIKPEHYPAVANALLPAIRDILGEAATDEILAAWGEAYWFLADILIAREAELYDEAQAA

Sequences:

>Translated_149_residues
MTKALSPQTMTLVKATAPALQQHGVDITTRMYQRLFVDPEIKALFDMAAQESGAQPKRLAAAILAFAQNVDKLDVLKPAI
ERIAGRHVDTHIKPEHYPAVANALLPAIRDILGEAATDEILAAWGEAYWFLADILIAREAELYDEAQAA
>Mature_148_residues
TKALSPQTMTLVKATAPALQQHGVDITTRMYQRLFVDPEIKALFDMAAQESGAQPKRLAAAILAFAQNVDKLDVLKPAIE
RIAGRHVDTHIKPEHYPAVANALLPAIRDILGEAATDEILAAWGEAYWFLADILIAREAELYDEAQAA

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG1017

COG function: function code C; Hemoglobin-like flavoprotein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788903, Length=142, Percent_Identity=42.2535211267606, Blast_Score=114, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6321673, Length=143, Percent_Identity=44.7552447552448, Blast_Score=118, Evalue=3e-28,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.17 [H]

Molecular weight: Translated: 16318; Mature: 16187

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS01033 GLOBIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKALSPQTMTLVKATAPALQQHGVDITTRMYQRLFVDPEIKALFDMAAQESGAQPKRLA
CCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHH
AAILAFAQNVDKLDVLKPAIERIAGRHVDTHIKPEHYPAVANALLPAIRDILGEAATDEI
HHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHH
LAAWGEAYWFLADILIAREAELYDEAQAA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKALSPQTMTLVKATAPALQQHGVDITTRMYQRLFVDPEIKALFDMAAQESGAQPKRLA
CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHH
AAILAFAQNVDKLDVLKPAIERIAGRHVDTHIKPEHYPAVANALLPAIRDILGEAATDEI
HHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHH
LAAWGEAYWFLADILIAREAELYDEAQAA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]