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The map label for this gene is pckG [H]

Identifier: 148550610

GI number: 148550610

Start: 64728

End: 66548

Strand: Reverse

Name: pckG [H]

Synonym: Swit_5171

Alternate gene names: 148550610

Gene position: 66548-64728 (Counterclockwise)

Preceding gene: 148550613

Following gene: 148550609

Centisome position: 21.45

GC content: 67.11

Gene sequence:

>1821_bases
ATGTCGGACGCCCTTATCGCTGAAACGCTGGCCGCCCCGACTCGCCATCGTCGCCTCGCCGCCTGGGTGGACGAGATCGC
CGCCCTTACCAAACCGGACCGGGTCCACTGGTGCGATGGCTCCGAGGCCGAGTGGGACGCGCTTACCGCGCAGCTCGTCG
CCGCCGGAACCCTGCGCCGCCTCGATCCCGCGAAGCGTCCCAACTCCTTCTGGGCCGCATCCGATCCACGCGACGTCGCG
CGCGTCGAAAGCCGCACCTTCATCTGCGCCGAGGACGAGGCCGACGCCGGCCCTACCAACAATTGGCGCGCACCGCATGA
GATGCGCGATACGCTCCACGGCCTATTCGACGGCTGCATGCGCGGACGCACCATGTATGTCGTGCCCTTCTCCATGGGGC
CGATCGGCTCGCCCATCAGCGCGCTCGGCGTGGAGATCACCGACAGTACCTATGTGGTGCTCTCGATGCGCGTGATGACG
AGGATGGGCGCACCGGCGCTGGCAGCCCTGGGCGATGACGGCGAATTCGTTCCCGCGGTCCACTCGGTCGGCGCTCCGCT
GGCGCCCGGCCAGGCCGACGTCGCCTGGCCCTGCAACGAGACCAAATATATCGTCCATTTCCCTGAAACGCGCGAGATCT
GGTCCTATGGATCGGGCTATGGCGGCAACGCCCTGCTAGGCAAGAAATGCTATGCGCTGCGCATCGCCTCGGTCATGGCC
CGCGACGACGGCTGGCTTGCTGAGCATATGCTGATCCTCAAGCTGACCTCGCCCGAAGGCGGCGTGCATTATGTCGCCGC
CGCTTTTCCCTCGGCCTGCGGCAAGACCAACCTCGCCATGCTGCAGCCAACCATTCCAGGCTGGAAGGCCGAAACGATCG
GCGACGACATCGCCTGGATGCGCTTTGGCGACGATGGCCGGCTCTATGCCATCAATCCCGAGGCCGGATTCTTCGGCGTC
GCACCCGGGACGAGCGCAACGACCAACGCCAATGCCCTGGCCAGCCTCAACGCCAACACGATCTTCACCAACACCGCGCT
CACCGCCGACGGTGATGTCTGGTGGGAGGGCCTGACGAAGGAGCCGCCCGACGGGCTTACGGACTGGCGCGGCCAGGCCT
GGTCTCCCGATCTCGGGACGCCGGCCGCCCATCCCAACGCGCGCTTCGCCTGTCCCGCCGGCCAGTGCCCGGCCATCGCA
CCCGAATGGGAGGATCCCAAGGGCGTCCCGATCTCGGCGATCCTGTTCGGCGGCCGGCGCGCGACGACCGCTCCGCTCGT
CACCGAGGCCTTCGACTGGCAGCACGGTGTGTTCCTGGCCTCGAATGTCGCCTCCGAAGGTACGGCTGCGGCCGAGACCA
AGATCGGGGATGTCCGCCGCGACCCCTTCGCGATGCTGCCCTTCTGCGGCTACAATATGGGCGACTACTTCGCGCATTGG
CTCAAGCTGGGAGCAGAAGCAGCGGATCCAGCCAAGCTGCCACGCATCTTCTACGTCAACTGGTTCCGCAAGGATGCCCA
TGGCCGCTTTCTCTGGCCCGGCTTCGGCGACAACGCCCGCGTCCTCAAATGGATCGCGGACCGGCTCGACGGGTGCGTCG
AGGCGGAAGACAAGGCCATCGGCCGCCTTCCCAATAGGCACGACCTCGATCTGCAGGGGCTCGATCTGTCGGATGACGCG
GTCACCGCCTTGCTCGCCGTCGATGACGATGAATGGCGCGCCGAGGCCGAGCGCATCCGCCGTGACTATGCGCGCTTCGG
CGACCGCCTGCCCGCCGCCTTGACCGCCGAACTCGGCGCGCTCGAAGCCCGTCTGGCGTGA

Upstream 100 bases:

>100_bases
ACTTGCTTTCAGCGCCATCAAACTGCTAACGCTCAGGCACCCAAACCACCTTTTTAACCGCGCGCGCTCGACGCGACGCC
CAGGTATTCGGAGAGTTTCG

Downstream 100 bases:

>100_bases
AGGGCGCCATCGATCGTGACCGACGCAAACGACGACTATGTCTATGACGAAGCCTCTGGCGACTGGATCACCGCTGCCGA
GGCAGCCGTGAATGCCCGGA

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 606; Mature: 605

Protein sequence:

>606_residues
MSDALIAETLAAPTRHRRLAAWVDEIAALTKPDRVHWCDGSEAEWDALTAQLVAAGTLRRLDPAKRPNSFWAASDPRDVA
RVESRTFICAEDEADAGPTNNWRAPHEMRDTLHGLFDGCMRGRTMYVVPFSMGPIGSPISALGVEITDSTYVVLSMRVMT
RMGAPALAALGDDGEFVPAVHSVGAPLAPGQADVAWPCNETKYIVHFPETREIWSYGSGYGGNALLGKKCYALRIASVMA
RDDGWLAEHMLILKLTSPEGGVHYVAAAFPSACGKTNLAMLQPTIPGWKAETIGDDIAWMRFGDDGRLYAINPEAGFFGV
APGTSATTNANALASLNANTIFTNTALTADGDVWWEGLTKEPPDGLTDWRGQAWSPDLGTPAAHPNARFACPAGQCPAIA
PEWEDPKGVPISAILFGGRRATTAPLVTEAFDWQHGVFLASNVASEGTAAAETKIGDVRRDPFAMLPFCGYNMGDYFAHW
LKLGAEAADPAKLPRIFYVNWFRKDAHGRFLWPGFGDNARVLKWIADRLDGCVEAEDKAIGRLPNRHDLDLQGLDLSDDA
VTALLAVDDDEWRAEAERIRRDYARFGDRLPAALTAELGALEARLA

Sequences:

>Translated_606_residues
MSDALIAETLAAPTRHRRLAAWVDEIAALTKPDRVHWCDGSEAEWDALTAQLVAAGTLRRLDPAKRPNSFWAASDPRDVA
RVESRTFICAEDEADAGPTNNWRAPHEMRDTLHGLFDGCMRGRTMYVVPFSMGPIGSPISALGVEITDSTYVVLSMRVMT
RMGAPALAALGDDGEFVPAVHSVGAPLAPGQADVAWPCNETKYIVHFPETREIWSYGSGYGGNALLGKKCYALRIASVMA
RDDGWLAEHMLILKLTSPEGGVHYVAAAFPSACGKTNLAMLQPTIPGWKAETIGDDIAWMRFGDDGRLYAINPEAGFFGV
APGTSATTNANALASLNANTIFTNTALTADGDVWWEGLTKEPPDGLTDWRGQAWSPDLGTPAAHPNARFACPAGQCPAIA
PEWEDPKGVPISAILFGGRRATTAPLVTEAFDWQHGVFLASNVASEGTAAAETKIGDVRRDPFAMLPFCGYNMGDYFAHW
LKLGAEAADPAKLPRIFYVNWFRKDAHGRFLWPGFGDNARVLKWIADRLDGCVEAEDKAIGRLPNRHDLDLQGLDLSDDA
VTALLAVDDDEWRAEAERIRRDYARFGDRLPAALTAELGALEARLA
>Mature_605_residues
SDALIAETLAAPTRHRRLAAWVDEIAALTKPDRVHWCDGSEAEWDALTAQLVAAGTLRRLDPAKRPNSFWAASDPRDVAR
VESRTFICAEDEADAGPTNNWRAPHEMRDTLHGLFDGCMRGRTMYVVPFSMGPIGSPISALGVEITDSTYVVLSMRVMTR
MGAPALAALGDDGEFVPAVHSVGAPLAPGQADVAWPCNETKYIVHFPETREIWSYGSGYGGNALLGKKCYALRIASVMAR
DDGWLAEHMLILKLTSPEGGVHYVAAAFPSACGKTNLAMLQPTIPGWKAETIGDDIAWMRFGDDGRLYAINPEAGFFGVA
PGTSATTNANALASLNANTIFTNTALTADGDVWWEGLTKEPPDGLTDWRGQAWSPDLGTPAAHPNARFACPAGQCPAIAP
EWEDPKGVPISAILFGGRRATTAPLVTEAFDWQHGVFLASNVASEGTAAAETKIGDVRRDPFAMLPFCGYNMGDYFAHWL
KLGAEAADPAKLPRIFYVNWFRKDAHGRFLWPGFGDNARVLKWIADRLDGCVEAEDKAIGRLPNRHDLDLQGLDLSDDAV
TALLAVDDDEWRAEAERIRRDYARFGDRLPAALTAELGALEARLA

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle [H]

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family [H]

Homologues:

Organism=Homo sapiens, GI66346721, Length=598, Percent_Identity=55.1839464882943, Blast_Score=637, Evalue=0.0,
Organism=Homo sapiens, GI187281517, Length=599, Percent_Identity=52.2537562604341, Blast_Score=629, Evalue=1e-180,
Organism=Homo sapiens, GI66346723, Length=370, Percent_Identity=57.027027027027, Blast_Score=418, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI71989645, Length=606, Percent_Identity=50.8250825082508, Blast_Score=599, Evalue=1e-171,
Organism=Caenorhabditis elegans, GI71989658, Length=606, Percent_Identity=50.8250825082508, Blast_Score=598, Evalue=1e-171,
Organism=Caenorhabditis elegans, GI17555492, Length=614, Percent_Identity=50.9771986970684, Blast_Score=593, Evalue=1e-170,
Organism=Caenorhabditis elegans, GI71989653, Length=593, Percent_Identity=51.433389544688, Blast_Score=592, Evalue=1e-169,
Organism=Caenorhabditis elegans, GI32564411, Length=537, Percent_Identity=54.0037243947858, Blast_Score=571, Evalue=1e-163,
Organism=Caenorhabditis elegans, GI32564407, Length=522, Percent_Identity=54.2145593869732, Blast_Score=545, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI71989649, Length=486, Percent_Identity=53.0864197530864, Blast_Score=513, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI32564409, Length=368, Percent_Identity=52.445652173913, Blast_Score=367, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17540956, Length=520, Percent_Identity=26.1538461538462, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24655082, Length=602, Percent_Identity=52.3255813953488, Blast_Score=620, Evalue=1e-177,
Organism=Drosophila melanogaster, GI24655085, Length=599, Percent_Identity=53.0884808013356, Blast_Score=617, Evalue=1e-177,
Organism=Drosophila melanogaster, GI161077226, Length=569, Percent_Identity=53.2513181019332, Blast_Score=592, Evalue=1e-169,
Organism=Drosophila melanogaster, GI24655088, Length=503, Percent_Identity=54.6719681908549, Blast_Score=548, Evalue=1e-156,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210 [H]

Pfam domain/function: PF00821 PEPCK [H]

EC number: =4.1.1.32 [H]

Molecular weight: Translated: 65718; Mature: 65586

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00505 PEPCK_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDALIAETLAAPTRHRRLAAWVDEIAALTKPDRVHWCDGSEAEWDALTAQLVAAGTLRR
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHH
LDPAKRPNSFWAASDPRDVARVESRTFICAEDEADAGPTNNWRAPHEMRDTLHGLFDGCM
CCCCCCCCCCCCCCCCHHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
RGRTMYVVPFSMGPIGSPISALGVEITDSTYVVLSMRVMTRMGAPALAALGDDGEFVPAV
CCCEEEEEEECCCCCCCCHHHHCEEECCCEEEEEEHHHHHHCCCCEEEEECCCCCEEHHH
HSVGAPLAPGQADVAWPCNETKYIVHFPETREIWSYGSGYGGNALLGKKCYALRIASVMA
HHCCCCCCCCCCCEEECCCCCEEEEECCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
RDDGWLAEHMLILKLTSPEGGVHYVAAAFPSACGKTNLAMLQPTIPGWKAETIGDDIAWM
CCCCCCEEEEEEEEEECCCCCEEEEEECCCHHCCCCCEEEEECCCCCCCCHHCCCCEEEE
RFGDDGRLYAINPEAGFFGVAPGTSATTNANALASLNANTIFTNTALTADGDVWWEGLTK
EECCCCEEEEECCCCCEEECCCCCCCCCCCCEEEECCCCEEEECCEEECCCCHHCCCCCC
EPPDGLTDWRGQAWSPDLGTPAAHPNARFACPAGQCPAIAPEWEDPKGVPISAILFGGRR
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCC
ATTAPLVTEAFDWQHGVFLASNVASEGTAAAETKIGDVRRDPFAMLPFCGYNMGDYFAHW
CCCCCHHHHHCCCCCCEEEECCCCCCCCHHHHHHHCCCCCCCCEECCCCCCCHHHHHHHH
LKLGAEAADPAKLPRIFYVNWFRKDAHGRFLWPGFGDNARVLKWIADRLDGCVEAEDKAI
HHHCCCCCCHHHCCEEEEEEEEEECCCCCEECCCCCCCHHHHHHHHHHHCCHHCCCHHHH
GRLPNRHDLDLQGLDLSDDAVTALLAVDDDEWRAEAERIRRDYARFGDRLPAALTAELGA
HCCCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LEARLA
HHHHCC
>Mature Secondary Structure 
SDALIAETLAAPTRHRRLAAWVDEIAALTKPDRVHWCDGSEAEWDALTAQLVAAGTLRR
CCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHH
LDPAKRPNSFWAASDPRDVARVESRTFICAEDEADAGPTNNWRAPHEMRDTLHGLFDGCM
CCCCCCCCCCCCCCCCHHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
RGRTMYVVPFSMGPIGSPISALGVEITDSTYVVLSMRVMTRMGAPALAALGDDGEFVPAV
CCCEEEEEEECCCCCCCCHHHHCEEECCCEEEEEEHHHHHHCCCCEEEEECCCCCEEHHH
HSVGAPLAPGQADVAWPCNETKYIVHFPETREIWSYGSGYGGNALLGKKCYALRIASVMA
HHCCCCCCCCCCCEEECCCCCEEEEECCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
RDDGWLAEHMLILKLTSPEGGVHYVAAAFPSACGKTNLAMLQPTIPGWKAETIGDDIAWM
CCCCCCEEEEEEEEEECCCCCEEEEEECCCHHCCCCCEEEEECCCCCCCCHHCCCCEEEE
RFGDDGRLYAINPEAGFFGVAPGTSATTNANALASLNANTIFTNTALTADGDVWWEGLTK
EECCCCEEEEECCCCCEEECCCCCCCCCCCCEEEECCCCEEEECCEEECCCCHHCCCCCC
EPPDGLTDWRGQAWSPDLGTPAAHPNARFACPAGQCPAIAPEWEDPKGVPISAILFGGRR
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCC
ATTAPLVTEAFDWQHGVFLASNVASEGTAAAETKIGDVRRDPFAMLPFCGYNMGDYFAHW
CCCCCHHHHHCCCCCCEEEECCCCCCCCHHHHHHHCCCCCCCCEECCCCCCCHHHHHHHH
LKLGAEAADPAKLPRIFYVNWFRKDAHGRFLWPGFGDNARVLKWIADRLDGCVEAEDKAI
HHHCCCCCCHHHCCEEEEEEEEEECCCCCEECCCCCCCHHHHHHHHHHHCCHHCCCHHHH
GRLPNRHDLDLQGLDLSDDAVTALLAVDDDEWRAEAERIRRDYARFGDRLPAALTAELGA
HCCCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LEARLA
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA