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The map label for this gene is yeiT [H]

Identifier: 148380779

GI number: 148380779

Start: 2989787

End: 2991103

Strand: Reverse

Name: yeiT [H]

Synonym: CBO2820

Alternate gene names: 148380779

Gene position: 2991103-2989787 (Counterclockwise)

Preceding gene: 148380780

Following gene: 148380778

Centisome position: 76.95

GC content: 33.03

Gene sequence:

>1317_bases
TTGGAAAATAAATTAACTTATATGAAAGAAAGGGAAGAAGTTTTTACACCTCTTCTAGCTATTGAAGAAGCATCAAGATG
TTTGCTATGTCATGATGCACCCTGTACAAAGGCATGTCCAGCAGGTACAAATCCCGGAAAATTCATACGTTCATTACGTT
TTAGAAATTTTAATGGGGCCGTAGCTACAATTCGTGAAAATAATATATTAGGAGGAGTCTGTGCAAGAGTCTGTCCTACA
GATAAATATTGTGAAGGAGCCTGTAGTAGATGTGGTATAGATAAGCCTATTCAAATAGGGAGACTTCAACGCTATTTAGC
AGATTATGAACAATCAACAGGAATAAAAATTTTAGAAGCTGTACAAGCTACAAAAGAAAAAGTAGCTATTATAGGTTCAG
GTCCAAGTGGACTTGCAGCGGCAGCTCAATTAGCTTTAGAAGGCTATAAAGTCACTGTATTTGAAGCTAAAAATCAATTA
GGTGGATGGTTAACTTATGGAATACCAGAAGATAGGCTTCCACAAAAAGTTGTTGAAAATGAAATTGGATACATAAAAAA
TTTAGGTGTCCATTTTAGAACTAACTGTAAAGTTGGAAGAGATGTTAGTATAGATGATCTTAGAAAAGAAGGTTTTAAAG
CATTTTTAGTAGCAGTAGGCATGCAAAAGAGTAAAGATATAGAAGTAAAAGGTAATAATTTAGAAGGAGTTGTTGCTGGA
ACAAATTTTTTAGCAGAAGCTAAAATTTCTAAGGGAAAAGTTAAAGTTGGCAGCAAAGTTATAGTTATAGGTGGAGGAGA
TGTTGCTATGGATTGTGCTGTAACTGCTAAATTATTAGGATCAGAAGATGTTAAGATAGTTTATAGAAGAACTATAGAAA
GAATGCCAGCAGATAGAAAGGAAATAGCTTATACCCAAGATTTAAATATACCAATATTTACAGGATTAAAGCCAGCTGAA
ATAATTGGACAAGCTGGAAAAGTGGATAGATTTAAAGCTACAGGAATGTTTGATGATTCAGAATTAAATCTTCCAGCAGA
TATGATTATATTTGCTATTGGACAAGAAGCAGAAGAAATAAAAGAATTAAAAGAAGCAGTAAAAGAAGTAGCTGCAACAA
AGGAAGTAACAATAGTAGAATTTAATGAAAAGGGAACAATTAAAACCCTAGATTATGCTACTAATATAGATGGGATTTTT
GCTTCAGGAGATATAATTGAAGGAGATAAAACTGTTGTAAATGCTGTAAAAGAAGGAAAAGAAGCAGCAAAGGCCATAGC
AAAATATTTATTAGCTAAGAAAGAAGGTGTAAGATAA

Upstream 100 bases:

>100_bases
AATAGACCATTTCTAAGTTAGAAATGGTCTATTTTTTTTGTTAAACTATTGTTATAACTGCAATTAACCTTTAAATATTA
AATATAAAGGAGGAATAATA

Downstream 100 bases:

>100_bases
TGAAAAGAAGAGATCTTTCCATAGAATTTTGTGGAGTAAAGTGTGAAAATCCATTTTTCTTATCATCATCACCAGTAGGA
AACTGTTACGAAATGTGTGC

Product: pyridine nucleotide-disulphide oxidoreductase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MENKLTYMKEREEVFTPLLAIEEASRCLLCHDAPCTKACPAGTNPGKFIRSLRFRNFNGAVATIRENNILGGVCARVCPT
DKYCEGACSRCGIDKPIQIGRLQRYLADYEQSTGIKILEAVQATKEKVAIIGSGPSGLAAAAQLALEGYKVTVFEAKNQL
GGWLTYGIPEDRLPQKVVENEIGYIKNLGVHFRTNCKVGRDVSIDDLRKEGFKAFLVAVGMQKSKDIEVKGNNLEGVVAG
TNFLAEAKISKGKVKVGSKVIVIGGGDVAMDCAVTAKLLGSEDVKIVYRRTIERMPADRKEIAYTQDLNIPIFTGLKPAE
IIGQAGKVDRFKATGMFDDSELNLPADMIIFAIGQEAEEIKELKEAVKEVAATKEVTIVEFNEKGTIKTLDYATNIDGIF
ASGDIIEGDKTVVNAVKEGKEAAKAIAKYLLAKKEGVR

Sequences:

>Translated_438_residues
MENKLTYMKEREEVFTPLLAIEEASRCLLCHDAPCTKACPAGTNPGKFIRSLRFRNFNGAVATIRENNILGGVCARVCPT
DKYCEGACSRCGIDKPIQIGRLQRYLADYEQSTGIKILEAVQATKEKVAIIGSGPSGLAAAAQLALEGYKVTVFEAKNQL
GGWLTYGIPEDRLPQKVVENEIGYIKNLGVHFRTNCKVGRDVSIDDLRKEGFKAFLVAVGMQKSKDIEVKGNNLEGVVAG
TNFLAEAKISKGKVKVGSKVIVIGGGDVAMDCAVTAKLLGSEDVKIVYRRTIERMPADRKEIAYTQDLNIPIFTGLKPAE
IIGQAGKVDRFKATGMFDDSELNLPADMIIFAIGQEAEEIKELKEAVKEVAATKEVTIVEFNEKGTIKTLDYATNIDGIF
ASGDIIEGDKTVVNAVKEGKEAAKAIAKYLLAKKEGVR
>Mature_438_residues
MENKLTYMKEREEVFTPLLAIEEASRCLLCHDAPCTKACPAGTNPGKFIRSLRFRNFNGAVATIRENNILGGVCARVCPT
DKYCEGACSRCGIDKPIQIGRLQRYLADYEQSTGIKILEAVQATKEKVAIIGSGPSGLAAAAQLALEGYKVTVFEAKNQL
GGWLTYGIPEDRLPQKVVENEIGYIKNLGVHFRTNCKVGRDVSIDDLRKEGFKAFLVAVGMQKSKDIEVKGNNLEGVVAG
TNFLAEAKISKGKVKVGSKVIVIGGGDVAMDCAVTAKLLGSEDVKIVYRRTIERMPADRKEIAYTQDLNIPIFTGLKPAE
IIGQAGKVDRFKATGMFDDSELNLPADMIIFAIGQEAEEIKELKEAVKEVAATKEVTIVEFNEKGTIKTLDYATNIDGIF
ASGDIIEGDKTVVNAVKEGKEAAKAIAKYLLAKKEGVR

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli glutamate synthase [NADPH] small chain (GltD) and to E.coli AegA [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=449, Percent_Identity=30.2895322939866, Blast_Score=182, Evalue=5e-46,
Organism=Homo sapiens, GI237757300, Length=87, Percent_Identity=43.6781609195402, Blast_Score=76, Evalue=7e-14,
Organism=Escherichia coli, GI1788468, Length=424, Percent_Identity=45.7547169811321, Blast_Score=370, Evalue=1e-104,
Organism=Escherichia coli, GI1788811, Length=454, Percent_Identity=31.9383259911894, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1789606, Length=445, Percent_Identity=31.0112359550562, Blast_Score=198, Evalue=5e-52,
Organism=Escherichia coli, GI87082180, Length=442, Percent_Identity=29.6380090497738, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1789243, Length=439, Percent_Identity=28.9293849658314, Blast_Score=138, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI71984108, Length=445, Percent_Identity=29.2134831460674, Blast_Score=169, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17570289, Length=465, Percent_Identity=29.0322580645161, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17543860, Length=209, Percent_Identity=28.2296650717703, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320030, Length=480, Percent_Identity=29.375, Blast_Score=142, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24640763, Length=437, Percent_Identity=31.1212814645309, Blast_Score=183, Evalue=2e-46,
Organism=Drosophila melanogaster, GI18858217, Length=437, Percent_Identity=31.1212814645309, Blast_Score=183, Evalue=2e-46,
Organism=Drosophila melanogaster, GI28574881, Length=466, Percent_Identity=29.6137339055794, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24665539, Length=466, Percent_Identity=29.6137339055794, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24665547, Length=466, Percent_Identity=29.6137339055794, Blast_Score=158, Evalue=6e-39,
Organism=Drosophila melanogaster, GI24665543, Length=466, Percent_Identity=29.6137339055794, Blast_Score=158, Evalue=6e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: NA

Molecular weight: Translated: 47565; Mature: 47565

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENKLTYMKEREEVFTPLLAIEEASRCLLCHDAPCTKACPAGTNPGKFIRSLRFRNFNGA
CCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCC
VATIRENNILGGVCARVCPTDKYCEGACSRCGIDKPIQIGRLQRYLADYEQSTGIKILEA
EEEEECCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHH
VQATKEKVAIIGSGPSGLAAAAQLALEGYKVTVFEAKNQLGGWLTYGIPEDRLPQKVVEN
HHHCCCEEEEEECCCCHHHHHHHHHHCCEEEEEEECCCCCCCEEEECCCCCCCHHHHHHH
EIGYIKNLGVHFRTNCKVGRDVSIDDLRKEGFKAFLVAVGMQKSKDIEVKGNNLEGVVAG
HHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHEEHHHHCCCCCCCEEEECCCCCEEEEC
TNFLAEAKISKGKVKVGSKVIVIGGGDVAMDCAVTAKLLGSEDVKIVYRRTIERMPADRK
CCEEEECCCCCCEEEECCEEEEEECCCEEEHHHHHHHHHCCCCHHHHHHHHHHHCCCCHH
EIAYTQDLNIPIFTGLKPAEIIGQAGKVDRFKATGMFDDSELNLPADMIIFAIGQEAEEI
HCEEEECCCCEEECCCCHHHHHCCCCCCCCEEECCCCCCCCCCCCHHHEEEEECCCHHHH
KELKEAVKEVAATKEVTIVEFNEKGTIKTLDYATNIDGIFASGDIIEGDKTVVNAVKEGK
HHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCCCEEECCCEECCCHHHHHHHHHHH
EAAKAIAKYLLAKKEGVR
HHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MENKLTYMKEREEVFTPLLAIEEASRCLLCHDAPCTKACPAGTNPGKFIRSLRFRNFNGA
CCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCC
VATIRENNILGGVCARVCPTDKYCEGACSRCGIDKPIQIGRLQRYLADYEQSTGIKILEA
EEEEECCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHH
VQATKEKVAIIGSGPSGLAAAAQLALEGYKVTVFEAKNQLGGWLTYGIPEDRLPQKVVEN
HHHCCCEEEEEECCCCHHHHHHHHHHCCEEEEEEECCCCCCCEEEECCCCCCCHHHHHHH
EIGYIKNLGVHFRTNCKVGRDVSIDDLRKEGFKAFLVAVGMQKSKDIEVKGNNLEGVVAG
HHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHEEHHHHCCCCCCCEEEECCCCCEEEEC
TNFLAEAKISKGKVKVGSKVIVIGGGDVAMDCAVTAKLLGSEDVKIVYRRTIERMPADRK
CCEEEECCCCCCEEEECCEEEEEECCCEEEHHHHHHHHHCCCCHHHHHHHHHHHCCCCHH
EIAYTQDLNIPIFTGLKPAEIIGQAGKVDRFKATGMFDDSELNLPADMIIFAIGQEAEEI
HCEEEECCCCEEECCCCHHHHHCCCCCCCCEEECCCCCCCCCCCCHHHEEEEECCCHHHH
KELKEAVKEVAATKEVTIVEFNEKGTIKTLDYATNIDGIFASGDIIEGDKTVVNAVKEGK
HHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCCCEEECCCEECCCHHHHHHHHHHH
EAAKAIAKYLLAKKEGVR
HHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]