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The map label for this gene is glpK

Identifier: 148380744

GI number: 148380744

Start: 2947271

End: 2948767

Strand: Reverse

Name: glpK

Synonym: CBO2784

Alternate gene names: 148380744

Gene position: 2948767-2947271 (Counterclockwise)

Preceding gene: 148380745

Following gene: 148380741

Centisome position: 75.86

GC content: 33.8

Gene sequence:

>1497_bases
ATGGAAAAGTATATAATGTCTTTAGATCAAGGAACTACTAGTTCAAGATGTATAATATTTAATAAAAAAGGTGAAATAGT
AAGTGTTGCTCAAAAGGAATTTACACAAATATATCCTAAAGCAGGATGGGTAGAACATGATCCACTAGAAATATGGGGAA
AACAAGCAGGGGTAGCAGGAGAGGCATTAAATATAGCTAGAATTTCTCCAGAACAAATAGCAGGTATAGGTATAACTAAT
CAAAGAGAAACTACAGTAGTTTGGAATAAAAGAACAGGAATGCCCGTATATAATGCTATAGTATGGCAATGTAGAAGAAC
AGCTGGTTATTGTGATGAATTAAGAGAAAAAGGTATAGATAAAACTATAAAAGAAAAAACAGGTCTAATGCTAGATGCCT
ATTTTTCAGCAACAAAAATAAAATGGATATTAGATAATGTAGAGGGAGCTAGAGAATTAGCAGAAAAAGGTGATTTGTTA
TTTGGAAATATAGATACTTGGTTAATCTGGAATATGACAAAGGGGAAAATTCATGTAACAGATTATACTAATGCTTCAAG
AACTATGTTATTTAACATACATGAATTAAAATGGGATGAAGAATTATTAGAAATACTTGATATTCCTAAATCTATGCTTC
CAGAAGTAAAACCATCTAGTTGTGTATATGGTGAAACAGATGAAATATTGTTCGGTGTATCAATTCCAATAGCCGGAGAT
GCAGGAGATCAACAAGCAGCTTTGTTTGGTCAAACTTGCTTTAATGCGGGTATGGCTAAAAATACTTATGGTACAGGTTG
TTTCTTGTTGATGAATACAGGAGAAAAAGCAGTAGATTCTAAAAATGGATTATTAACTACTATAGCTGTGGGTATAGATG
GAAAAGTTGAATATGCTTTAGAAGGAAGTATATTTATAGGTGGAGCAGTAATTCAATGGCTAAGAGATGAACTTAGAATG
GTAAAAACTGCTCAAGAAACAGAAAAATATGCCACAGAAGTAGAAGATAATAATGGAGTTTACCTTGTGCCAGCTTTTGT
CGGAATAGGTGCACCATACTGGGATTCTTATGCAAGGGGAACTATATTAGGCCTTACAAGAGGAGCTAAAAAAGAACACA
TAATAAGAGCTGCATTAGAATCTATGGCATACCAAACTCATGATGTTTTAAAAGCTATGGAAGAGGACTCAGGTATAGAA
CTAAAAGCATTAAAAGTAGATGGGGGAGCCTGTCAAAATAATTTCTTAATGCAATTTCAATCTGATATATTAGGTGTAGA
AGTAGATAGACCAGAAGTAGTAGAGACTACAGCATTAGGAGCAGCTTATCTTGCAGGACTTGCAGTAGGATATTGGAAGG
ATAGAAATGAAATATCACAAAACTGGGCAATTTCAAGAAGCTTTGCTCCAGCGATGGAAGATGAAAAGAAAGAAAAACTT
ATAAAAGGATGGCATAAGGCAGTAACAAAAGCAATGGATTGGGAAGAAAGAGAATAA

Upstream 100 bases:

>100_bases
AGTTTAAATATAAGAAAGTTATGTTTTAAAAGATTATTTTACTAGTTTTTTTATCTTAATATTTTATTAAATGAATAAAA
AAGGGAAGGTGGAGTTTATA

Downstream 100 bases:

>100_bases
TATAAGGAAATTTATAGCTTAAAAGGTAGGTAGCTTCAGCTTATTAAAAAGGAATATGTGACGAGATCGGCAGCTCAATT
CACTAAGATATTCTAAAATT

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MEKYIMSLDQGTTSSRCIIFNKKGEIVSVAQKEFTQIYPKAGWVEHDPLEIWGKQAGVAGEALNIARISPEQIAGIGITN
QRETTVVWNKRTGMPVYNAIVWQCRRTAGYCDELREKGIDKTIKEKTGLMLDAYFSATKIKWILDNVEGARELAEKGDLL
FGNIDTWLIWNMTKGKIHVTDYTNASRTMLFNIHELKWDEELLEILDIPKSMLPEVKPSSCVYGETDEILFGVSIPIAGD
AGDQQAALFGQTCFNAGMAKNTYGTGCFLLMNTGEKAVDSKNGLLTTIAVGIDGKVEYALEGSIFIGGAVIQWLRDELRM
VKTAQETEKYATEVEDNNGVYLVPAFVGIGAPYWDSYARGTILGLTRGAKKEHIIRAALESMAYQTHDVLKAMEEDSGIE
LKALKVDGGACQNNFLMQFQSDILGVEVDRPEVVETTALGAAYLAGLAVGYWKDRNEISQNWAISRSFAPAMEDEKKEKL
IKGWHKAVTKAMDWEERE

Sequences:

>Translated_498_residues
MEKYIMSLDQGTTSSRCIIFNKKGEIVSVAQKEFTQIYPKAGWVEHDPLEIWGKQAGVAGEALNIARISPEQIAGIGITN
QRETTVVWNKRTGMPVYNAIVWQCRRTAGYCDELREKGIDKTIKEKTGLMLDAYFSATKIKWILDNVEGARELAEKGDLL
FGNIDTWLIWNMTKGKIHVTDYTNASRTMLFNIHELKWDEELLEILDIPKSMLPEVKPSSCVYGETDEILFGVSIPIAGD
AGDQQAALFGQTCFNAGMAKNTYGTGCFLLMNTGEKAVDSKNGLLTTIAVGIDGKVEYALEGSIFIGGAVIQWLRDELRM
VKTAQETEKYATEVEDNNGVYLVPAFVGIGAPYWDSYARGTILGLTRGAKKEHIIRAALESMAYQTHDVLKAMEEDSGIE
LKALKVDGGACQNNFLMQFQSDILGVEVDRPEVVETTALGAAYLAGLAVGYWKDRNEISQNWAISRSFAPAMEDEKKEKL
IKGWHKAVTKAMDWEERE
>Mature_498_residues
MEKYIMSLDQGTTSSRCIIFNKKGEIVSVAQKEFTQIYPKAGWVEHDPLEIWGKQAGVAGEALNIARISPEQIAGIGITN
QRETTVVWNKRTGMPVYNAIVWQCRRTAGYCDELREKGIDKTIKEKTGLMLDAYFSATKIKWILDNVEGARELAEKGDLL
FGNIDTWLIWNMTKGKIHVTDYTNASRTMLFNIHELKWDEELLEILDIPKSMLPEVKPSSCVYGETDEILFGVSIPIAGD
AGDQQAALFGQTCFNAGMAKNTYGTGCFLLMNTGEKAVDSKNGLLTTIAVGIDGKVEYALEGSIFIGGAVIQWLRDELRM
VKTAQETEKYATEVEDNNGVYLVPAFVGIGAPYWDSYARGTILGLTRGAKKEHIIRAALESMAYQTHDVLKAMEEDSGIE
LKALKVDGGACQNNFLMQFQSDILGVEVDRPEVVETTALGAAYLAGLAVGYWKDRNEISQNWAISRSFAPAMEDEKKEKL
IKGWHKAVTKAMDWEERE

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI189409120, Length=502, Percent_Identity=49.203187250996, Blast_Score=436, Evalue=1e-122,
Organism=Homo sapiens, GI4504007, Length=502, Percent_Identity=49.203187250996, Blast_Score=435, Evalue=1e-122,
Organism=Homo sapiens, GI42794763, Length=506, Percent_Identity=48.4189723320158, Blast_Score=435, Evalue=1e-122,
Organism=Homo sapiens, GI41393575, Length=499, Percent_Identity=47.4949899799599, Blast_Score=427, Evalue=1e-119,
Organism=Homo sapiens, GI88196792, Length=510, Percent_Identity=34.1176470588235, Blast_Score=338, Evalue=9e-93,
Organism=Escherichia coli, GI1790361, Length=498, Percent_Identity=64.0562248995984, Blast_Score=686, Evalue=0.0,
Organism=Escherichia coli, GI1787789, Length=480, Percent_Identity=24.375, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789168, Length=498, Percent_Identity=23.8955823293173, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1789987, Length=482, Percent_Identity=23.4439834024896, Blast_Score=89, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17535599, Length=504, Percent_Identity=42.0634920634921, Blast_Score=397, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI71986140, Length=518, Percent_Identity=25.8687258687259, Blast_Score=188, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI71986147, Length=271, Percent_Identity=29.8892988929889, Blast_Score=125, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6321755, Length=554, Percent_Identity=37.3646209386282, Blast_Score=322, Evalue=8e-89,
Organism=Drosophila melanogaster, GI17864214, Length=512, Percent_Identity=42.1875, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24655555, Length=482, Percent_Identity=40.0414937759336, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655560, Length=482, Percent_Identity=40.0414937759336, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655570, Length=482, Percent_Identity=40.0414937759336, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655574, Length=482, Percent_Identity=40.0414937759336, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655565, Length=482, Percent_Identity=40.0414937759336, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24654573, Length=441, Percent_Identity=43.7641723356009, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI19922058, Length=533, Percent_Identity=34.1463414634146, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI24652871, Length=498, Percent_Identity=34.3373493975904, Blast_Score=275, Evalue=4e-74,
Organism=Drosophila melanogaster, GI24656367, Length=514, Percent_Identity=27.6264591439689, Blast_Score=225, Evalue=6e-59,
Organism=Drosophila melanogaster, GI21357867, Length=530, Percent_Identity=26.9811320754717, Blast_Score=219, Evalue=2e-57,
Organism=Drosophila melanogaster, GI24656372, Length=349, Percent_Identity=28.6532951289398, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI45552909, Length=290, Percent_Identity=27.2413793103448, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_CLOB1 (A7FX30)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001385028.1
- ProteinModelPortal:   A7FX30
- SMR:   A7FX30
- STRING:   A7FX30
- GeneID:   5395946
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_2727
- eggNOG:   COG0554
- HOGENOM:   HBG511469
- OMA:   DSGVRLE
- ProtClustDB:   CLSK961165
- BioCyc:   CBOT441770:CLB_2727-MONOMER
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 55392; Mature: 55392

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 12-12 BINDING 16-16 BINDING 82-82 BINDING 134-134 BINDING 243-243 BINDING 265-265 BINDING 308-308

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKYIMSLDQGTTSSRCIIFNKKGEIVSVAQKEFTQIYPKAGWVEHDPLEIWGKQAGVAG
CCCCEEEECCCCCCCEEEEECCCCCEEHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCC
EALNIARISPEQIAGIGITNQRETTVVWNKRTGMPVYNAIVWQCRRTAGYCDELREKGID
CCEEEEECCHHHHEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHCCCC
KTIKEKTGLMLDAYFSATKIKWILDNVEGARELAEKGDLLFGNIDTWLIWNMTKGKIHVT
HHHHHHCCCEEEEECCHHEEEEEHHCHHHHHHHHHCCCEEEECCCEEEEEEECCCEEEEE
DYTNASRTMLFNIHELKWDEELLEILDIPKSMLPEVKPSSCVYGETDEILFGVSIPIAGD
ECCCCCCEEEEEEHHHCCHHHHHHHHCCCHHHCCCCCCCCEECCCCCCEEEEEEEEECCC
AGDQQAALFGQTCFNAGMAKNTYGTGCFLLMNTGEKAVDSKNGLLTTIAVGIDGKVEYAL
CCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCHHHCCCCCEEEEEEEECCCEEEEEE
EGSIFIGGAVIQWLRDELRMVKTAQETEKYATEVEDNNGVYLVPAFVGIGAPYWDSYARG
ECCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHCCCCHHHHHCCC
TILGLTRGAKKEHIIRAALESMAYQTHDVLKAMEEDSGIELKALKVDGGACQNNFLMQFQ
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHH
SDILGVEVDRPEVVETTALGAAYLAGLAVGYWKDRNEISQNWAISRSFAPAMEDEKKEKL
HCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCEECCCCCCCCCHHHHHHH
IKGWHKAVTKAMDWEERE
HHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MEKYIMSLDQGTTSSRCIIFNKKGEIVSVAQKEFTQIYPKAGWVEHDPLEIWGKQAGVAG
CCCCEEEECCCCCCCEEEEECCCCCEEHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCC
EALNIARISPEQIAGIGITNQRETTVVWNKRTGMPVYNAIVWQCRRTAGYCDELREKGID
CCEEEEECCHHHHEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHCCCC
KTIKEKTGLMLDAYFSATKIKWILDNVEGARELAEKGDLLFGNIDTWLIWNMTKGKIHVT
HHHHHHCCCEEEEECCHHEEEEEHHCHHHHHHHHHCCCEEEECCCEEEEEEECCCEEEEE
DYTNASRTMLFNIHELKWDEELLEILDIPKSMLPEVKPSSCVYGETDEILFGVSIPIAGD
ECCCCCCEEEEEEHHHCCHHHHHHHHCCCHHHCCCCCCCCEECCCCCCEEEEEEEEECCC
AGDQQAALFGQTCFNAGMAKNTYGTGCFLLMNTGEKAVDSKNGLLTTIAVGIDGKVEYAL
CCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCHHHCCCCCEEEEEEEECCCEEEEEE
EGSIFIGGAVIQWLRDELRMVKTAQETEKYATEVEDNNGVYLVPAFVGIGAPYWDSYARG
ECCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHCCCCHHHHHCCC
TILGLTRGAKKEHIIRAALESMAYQTHDVLKAMEEDSGIELKALKVDGGACQNNFLMQFQ
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHH
SDILGVEVDRPEVVETTALGAAYLAGLAVGYWKDRNEISQNWAISRSFAPAMEDEKKEKL
HCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCEECCCCCCCCCHHHHHHH
IKGWHKAVTKAMDWEERE
HHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA