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The map label for this gene is glpF [H]

Identifier: 148380745

GI number: 148380745

Start: 2949031

End: 2949735

Strand: Reverse

Name: glpF [H]

Synonym: CBO2785

Alternate gene names: 148380745

Gene position: 2949735-2949031 (Counterclockwise)

Preceding gene: 148380747

Following gene: 148380744

Centisome position: 75.89

GC content: 39.01

Gene sequence:

>705_bases
ATGTCAATTTATATGGCGGAGTTTTTGGGTACTATGATGTTAATTTGGTTAGGGGATGGCGTTGTAGCCAGTGTAGCTCT
TAACAAAAGTAAGGGAAAGGATGGTGGCTGGATTGTTGTAACAGCGGCCTGGGGATTAGCTGTTGCTATACCTGCATATA
TTTTTGGAGGTATAAGTGGAGCTCATATGAATCCAGCAGTTACAATTGGTAATGCAATTGCAGGAAATTTCCCTTGGGCA
AATGTAGCAGGTTATATTACAGCTCAAATGTTAGGGGGGTTTGCAGGAGCAGTTTTAGTTTGGCTTACTTACCTTCCACA
TTGGAAAGCAACAGAAGATAAAGCTACTAAGCTGGGAGTATTTTGTACAGCTCCAGCAATAAGAGACACAAAAGCTAACT
TTGTAACTGAATTTATAGTAACTGCCCTTTTAGTATTTGGACTAATGGGTCTTGGACAAGTTAAAATGGTAGATGGCTTT
GGACCATTAACTGCAGGTGCACTTATATTTGTTTTGGGACTTTCTTTAGGTGGACCAACAGGATATGCTATTAATCCCGC
AAGAGATTTAGCACCAAGAATAGCTCATGCAATTTTACCAATAGCGGGTAAAGGAGATTCAGATTGGAAATATGCATGGA
TACCAGTGCTAGGTCCAATATTAGGAGGAATATTAGGAGCATTATTATTTATGTTAGTTTTTTAA

Upstream 100 bases:

>100_bases
GGCGGAGATAAGAGAGCCAATTCATTACGTTTCAATGGGTGAGTTGACTCTATTTTGTCTTTTCCAAAATTAAATAATTT
TAACAGGGGGAGTAAAAATT

Downstream 100 bases:

>100_bases
TTAAAACTTTATGAAAACATTTAAGTAAATTATTTCATCATGATATAATAAAGCAAAAATAATACTAATGAATTATATAA
TAGAAAAATATTTAAAAATT

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MSIYMAEFLGTMMLIWLGDGVVASVALNKSKGKDGGWIVVTAAWGLAVAIPAYIFGGISGAHMNPAVTIGNAIAGNFPWA
NVAGYITAQMLGGFAGAVLVWLTYLPHWKATEDKATKLGVFCTAPAIRDTKANFVTEFIVTALLVFGLMGLGQVKMVDGF
GPLTAGALIFVLGLSLGGPTGYAINPARDLAPRIAHAILPIAGKGDSDWKYAWIPVLGPILGGILGALLFMLVF

Sequences:

>Translated_234_residues
MSIYMAEFLGTMMLIWLGDGVVASVALNKSKGKDGGWIVVTAAWGLAVAIPAYIFGGISGAHMNPAVTIGNAIAGNFPWA
NVAGYITAQMLGGFAGAVLVWLTYLPHWKATEDKATKLGVFCTAPAIRDTKANFVTEFIVTALLVFGLMGLGQVKMVDGF
GPLTAGALIFVLGLSLGGPTGYAINPARDLAPRIAHAILPIAGKGDSDWKYAWIPVLGPILGGILGALLFMLVF
>Mature_233_residues
SIYMAEFLGTMMLIWLGDGVVASVALNKSKGKDGGWIVVTAAWGLAVAIPAYIFGGISGAHMNPAVTIGNAIAGNFPWAN
VAGYITAQMLGGFAGAVLVWLTYLPHWKATEDKATKLGVFCTAPAIRDTKANFVTEFIVTALLVFGLMGLGQVKMVDGFG
PLTAGALIFVLGLSLGGPTGYAINPARDLAPRIAHAILPIAGKGDSDWKYAWIPVLGPILGGILGALLFMLVF

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI157266307, Length=245, Percent_Identity=36.3265306122449, Blast_Score=127, Evalue=6e-30,
Organism=Homo sapiens, GI4826645, Length=244, Percent_Identity=34.8360655737705, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI4502187, Length=244, Percent_Identity=33.1967213114754, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI22538420, Length=245, Percent_Identity=31.4285714285714, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI4502179, Length=230, Percent_Identity=30.8695652173913, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI310133356, Length=221, Percent_Identity=29.8642533936652, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI310114181, Length=221, Percent_Identity=29.8642533936652, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4502181, Length=204, Percent_Identity=28.921568627451, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI4755125, Length=204, Percent_Identity=28.921568627451, Blast_Score=66, Evalue=2e-11,
Organism=Escherichia coli, GI1790362, Length=246, Percent_Identity=35.7723577235772, Blast_Score=113, Evalue=9e-27,
Organism=Escherichia coli, GI1787101, Length=191, Percent_Identity=31.413612565445, Blast_Score=61, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI71994009, Length=233, Percent_Identity=32.1888412017167, Blast_Score=110, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17544068, Length=242, Percent_Identity=32.6446280991736, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71992966, Length=245, Percent_Identity=28.9795918367347, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17531429, Length=241, Percent_Identity=28.6307053941909, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17531431, Length=241, Percent_Identity=28.6307053941909, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17558372, Length=234, Percent_Identity=28.6324786324786, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71992961, Length=234, Percent_Identity=28.2051282051282, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI32564052, Length=239, Percent_Identity=25.9414225941423, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17533613, Length=237, Percent_Identity=26.1603375527426, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI71993722, Length=230, Percent_Identity=29.5652173913043, Blast_Score=67, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6321054, Length=206, Percent_Identity=36.4077669902913, Blast_Score=103, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6322985, Length=280, Percent_Identity=27.8571428571429, Blast_Score=92, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24652747, Length=234, Percent_Identity=29.0598290598291, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI45551084, Length=234, Percent_Identity=29.0598290598291, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19922828, Length=196, Percent_Identity=29.5918367346939, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24762342, Length=242, Percent_Identity=25.2066115702479, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 24381; Mature: 24250

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIYMAEFLGTMMLIWLGDGVVASVALNKSKGKDGGWIVVTAAWGLAVAIPAYIFGGISG
CCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCC
AHMNPAVTIGNAIAGNFPWANVAGYITAQMLGGFAGAVLVWLTYLPHWKATEDKATKLGV
CCCCCCEEECCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHEEEE
FCTAPAIRDTKANFVTEFIVTALLVFGLMGLGQVKMVDGFGPLTAGALIFVLGLSLGGPT
EEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHCCCCC
GYAINPARDLAPRIAHAILPIAGKGDSDWKYAWIPVLGPILGGILGALLFMLVF
CCEECCHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SIYMAEFLGTMMLIWLGDGVVASVALNKSKGKDGGWIVVTAAWGLAVAIPAYIFGGISG
CCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCC
AHMNPAVTIGNAIAGNFPWANVAGYITAQMLGGFAGAVLVWLTYLPHWKATEDKATKLGV
CCCCCCEEECCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHEEEE
FCTAPAIRDTKANFVTEFIVTALLVFGLMGLGQVKMVDGFGPLTAGALIFVLGLSLGGPT
EEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHCCCCC
GYAINPARDLAPRIAHAILPIAGKGDSDWKYAWIPVLGPILGGILGALLFMLVF
CCEECCHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]