The gene/protein map for NC_009495 is currently unavailable.
Definition Clostridium botulinum A str. ATCC 3502, complete genome.
Accession NC_009495
Length 3,886,916

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The map label for this gene is cheB [H]

Identifier: 148380708

GI number: 148380708

Start: 2900138

End: 2901202

Strand: Reverse

Name: cheB [H]

Synonym: CBO2750

Alternate gene names: 148380708

Gene position: 2901202-2900138 (Counterclockwise)

Preceding gene: 148380709

Following gene: 148380707

Centisome position: 74.64

GC content: 30.8

Gene sequence:

>1065_bases
TTGAATAAAATTAAAGTTTTAGTAGTAGATGACTCAGCTTTAATGAGAAAAATAGTATCAGATATGATAAATGAAGAAAA
TGATTTAGAAGTAATAGATACAGCTAGAAATGGAGAAGATCTTTTAGAAAAGTTGAAGAGGATAAAGCCAAATATAATAA
CATTAGATGTGGAAATGCCTAAAATGGATGGAATAGAAGCTTTAATAAATATTAAAAGGATTTACAAAGATATACCGGTT
ATTATGCTTAGTAGTTTATCTAAAAAAGGTGCAGGATTAACTATGGATTGTCTTCAAAAGGGAGCCTTTGATTTTGTACC
TAAGCCATCAGGGGCTATATCTTTAGATATAAATAAAGTAAAAGAAGAATTAGTAGAAAAAATAAGAGCTGCTACTAATG
CTATAAAAAATAATTACACTTCAGATATTTTAAAAACAGACGAAAAAATTAATGTTAAAAAAGTGGAAGAAAAAAGATAT
CCTATAAAAAATACTATTTCTTCAAAAAAAATAGAAGCTGTAGTTATAGGAGCTTCAACAGGAGGCCCTAAAGCATTATA
TTCAGTAATAACAGCTTTTCCAGAAAAAATAGATGTGCCAGTATTTGTAGTTCAACATATGCCAAAAGGATTTACCAAAG
CTTTTTCAGAAAGACTAAATGCTAATAGTAAATTAAAAGTTGTAGAAGGGCAAGATGGACAAATTATAGAGAAAGATACT
GTTTATGTAGCCCCAGGAGGCTTTCATATGGAAGTAGGAAAGGATAGAAAAATACATCTAAACATAGAACCAACCCTATG
GGGAGTGAGACCAGCTGTAGATAAGTTATTTAAATCAGCATCTGAAGTTTATGGCAGCAACTTAATAAGTGTTGTATTAA
CTGGTATGGGAAGAGATGGCGCAGATGGTACAGCCTATATAAAAGATAGAGGAGGCATAACCATATCAGAAGATGAATCT
ACCTGTACTATATACGGTATGCCAAAAGCAACTTACGAAACAGGAAAGGTGGATTTCGTAGTACAACTTTATAATGTTAC
AGATACAATACTGAAAAATATTTAA

Upstream 100 bases:

>100_bases
GGAAGAACTATGATATTTGATACCTCCACAGGAAAAGTATATATAAAGACTGTAGGCTTAGGAACTAAAGAAATATAGAT
AAGTTATTGGAGTGATTCAT

Downstream 100 bases:

>100_bases
TTTTGGAGGTAATTATGGATTTAGAATATTTCGAGGAGTGGGTATTAAAGGATTTTGGAATAAATTTAAAAGCATATAAA
CAAAATCAGCTTCAAAGAAG

Product: protein-glutamate methylesterase CheB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MNKIKVLVVDDSALMRKIVSDMINEENDLEVIDTARNGEDLLEKLKRIKPNIITLDVEMPKMDGIEALINIKRIYKDIPV
IMLSSLSKKGAGLTMDCLQKGAFDFVPKPSGAISLDINKVKEELVEKIRAATNAIKNNYTSDILKTDEKINVKKVEEKRY
PIKNTISSKKIEAVVIGASTGGPKALYSVITAFPEKIDVPVFVVQHMPKGFTKAFSERLNANSKLKVVEGQDGQIIEKDT
VYVAPGGFHMEVGKDRKIHLNIEPTLWGVRPAVDKLFKSASEVYGSNLISVVLTGMGRDGADGTAYIKDRGGITISEDES
TCTIYGMPKATYETGKVDFVVQLYNVTDTILKNI

Sequences:

>Translated_354_residues
MNKIKVLVVDDSALMRKIVSDMINEENDLEVIDTARNGEDLLEKLKRIKPNIITLDVEMPKMDGIEALINIKRIYKDIPV
IMLSSLSKKGAGLTMDCLQKGAFDFVPKPSGAISLDINKVKEELVEKIRAATNAIKNNYTSDILKTDEKINVKKVEEKRY
PIKNTISSKKIEAVVIGASTGGPKALYSVITAFPEKIDVPVFVVQHMPKGFTKAFSERLNANSKLKVVEGQDGQIIEKDT
VYVAPGGFHMEVGKDRKIHLNIEPTLWGVRPAVDKLFKSASEVYGSNLISVVLTGMGRDGADGTAYIKDRGGITISEDES
TCTIYGMPKATYETGKVDFVVQLYNVTDTILKNI
>Mature_354_residues
MNKIKVLVVDDSALMRKIVSDMINEENDLEVIDTARNGEDLLEKLKRIKPNIITLDVEMPKMDGIEALINIKRIYKDIPV
IMLSSLSKKGAGLTMDCLQKGAFDFVPKPSGAISLDINKVKEELVEKIRAATNAIKNNYTSDILKTDEKINVKKVEEKRY
PIKNTISSKKIEAVVIGASTGGPKALYSVITAFPEKIDVPVFVVQHMPKGFTKAFSERLNANSKLKVVEGQDGQIIEKDT
VYVAPGGFHMEVGKDRKIHLNIEPTLWGVRPAVDKLFKSASEVYGSNLISVVLTGMGRDGADGTAYIKDRGGITISEDES
TCTIYGMPKATYETGKVDFVVQLYNVTDTILKNI

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR [H]

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788192, Length=358, Percent_Identity=37.4301675977654, Blast_Score=237, Evalue=8e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789 [H]

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg [H]

EC number: =3.1.1.61 [H]

Molecular weight: Translated: 39055; Mature: 39055

Theoretical pI: Translated: 8.55; Mature: 8.55

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50122 CHEB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKIKVLVVDDSALMRKIVSDMINEENDLEVIDTARNGEDLLEKLKRIKPNIITLDVEMP
CCCEEEEEECCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHCCCCEEEEEEECC
KMDGIEALINIKRIYKDIPVIMLSSLSKKGAGLTMDCLQKGAFDFVPKPSGAISLDINKV
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCEEEEEHHHH
KEELVEKIRAATNAIKNNYTSDILKTDEKINVKKVEEKRYPIKNTISSKKIEAVVIGAST
HHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCEEHHHHCCCHHHCCCCCCEEEEEEECCC
GGPKALYSVITAFPEKIDVPVFVVQHMPKGFTKAFSERLNANSKLKVVEGQDGQIIEKDT
CCHHHHHHHHHHCHHHCCCCEEEEHHCCCHHHHHHHHHHCCCCEEEEEECCCCCEEECCE
VYVAPGGFHMEVGKDRKIHLNIEPTLWGVRPAVDKLFKSASEVYGSNLISVVLTGMGRDG
EEECCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCHHEEHHHCCCCCCC
ADGTAYIKDRGGITISEDESTCTIYGMPKATYETGKVDFVVQLYNVTDTILKNI
CCCEEEEECCCCEEEECCCCEEEEEECCCCCCCCCCEEEEEEEEHHHHHHHHCC
>Mature Secondary Structure
MNKIKVLVVDDSALMRKIVSDMINEENDLEVIDTARNGEDLLEKLKRIKPNIITLDVEMP
CCCEEEEEECCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHCCCCEEEEEEECC
KMDGIEALINIKRIYKDIPVIMLSSLSKKGAGLTMDCLQKGAFDFVPKPSGAISLDINKV
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCEEEEEHHHH
KEELVEKIRAATNAIKNNYTSDILKTDEKINVKKVEEKRYPIKNTISSKKIEAVVIGAST
HHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCEEHHHHCCCHHHCCCCCCEEEEEEECCC
GGPKALYSVITAFPEKIDVPVFVVQHMPKGFTKAFSERLNANSKLKVVEGQDGQIIEKDT
CCHHHHHHHHHHCHHHCCCCEEEEHHCCCHHHHHHHHHHCCCCEEEEEECCCCCEEECCE
VYVAPGGFHMEVGKDRKIHLNIEPTLWGVRPAVDKLFKSASEVYGSNLISVVLTGMGRDG
EEECCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCHHEEHHHCCCCCCC
ADGTAYIKDRGGITISEDESTCTIYGMPKATYETGKVDFVVQLYNVTDTILKNI
CCCEEEEECCCCEEEECCCCEEEEEECCCCCCCCCCEEEEEEEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]