Definition Clostridium botulinum A str. ATCC 3502, complete genome.
Accession NC_009495
Length 3,886,916

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The map label for this gene is cheD

Identifier: 148380709

GI number: 148380709

Start: 2901225

End: 2901713

Strand: Reverse

Name: cheD

Synonym: CBO2751

Alternate gene names: 148380709

Gene position: 2901713-2901225 (Counterclockwise)

Preceding gene: 148380710

Following gene: 148380708

Centisome position: 74.65

GC content: 32.72

Gene sequence:

>489_bases
ATGGATATAAAAGAGATAAAAGTGGGTATTGCAGATTTAAATGTAGGTAAAAATCCTGATAAGATAATAACAGTAGGACT
AGGCTCCTGTATAGGAATAGCATTATATGATGGCATAAAATGTATAGGGGGGCTTTCGCATATAATGTTACCGGATAGCA
CTCAATTTAGCAAAGTTACAAATCCTATGAAGTTCGCAGATTTAGCTATACCTATATTAGTAGAAAAGATGGAAAAATTA
GGAGCAAGGAAGAATGGATTAAAAGCTAAAATATGTGGCGGAGCATCTATGTTTAATTTTTCAGATAAAAGTATGGTAAT
GGATATAGGAAATAGAAATGGGAAAGCAGTAAAGGAAAAATTAAAGGAACTATCTATACCTTTGCTTGCAGAAGATATAG
GGGGAAACAAAGGAAGAACTATGATATTTGATACCTCCACAGGAAAAGTATATATAAAGACTGTAGGCTTAGGAACTAAA
GAAATATAG

Upstream 100 bases:

>100_bases
GGATTAATAAAAATAGAAAATCAAATAATAATATTTTTAAATTTATCAACTATACTAACAGAAGAAGAAAAATCAATGAT
ATAGTTGAGGGAGTAATTGT

Downstream 100 bases:

>100_bases
ATAAGTTATTGGAGTGATTCATTTGAATAAAATTAAAGTTTTAGTAGTAGATGACTCAGCTTTAATGAGAAAAATAGTAT
CAGATATGATAAATGAAGAA

Product: chemoreceptor glutamine deamidase CheD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MDIKEIKVGIADLNVGKNPDKIITVGLGSCIGIALYDGIKCIGGLSHIMLPDSTQFSKVTNPMKFADLAIPILVEKMEKL
GARKNGLKAKICGGASMFNFSDKSMVMDIGNRNGKAVKEKLKELSIPLLAEDIGGNKGRTMIFDTSTGKVYIKTVGLGTK
EI

Sequences:

>Translated_162_residues
MDIKEIKVGIADLNVGKNPDKIITVGLGSCIGIALYDGIKCIGGLSHIMLPDSTQFSKVTNPMKFADLAIPILVEKMEKL
GARKNGLKAKICGGASMFNFSDKSMVMDIGNRNGKAVKEKLKELSIPLLAEDIGGNKGRTMIFDTSTGKVYIKTVGLGTK
EI
>Mature_162_residues
MDIKEIKVGIADLNVGKNPDKIITVGLGSCIGIALYDGIKCIGGLSHIMLPDSTQFSKVTNPMKFADLAIPILVEKMEKL
GARKNGLKAKICGGASMFNFSDKSMVMDIGNRNGKAVKEKLKELSIPLLAEDIGGNKGRTMIFDTSTGKVYIKTVGLGTK
EI

Specific function: Probably deamidates glutamine residues to glutamate on methyl-accepting chemotaxis receptors (MCPs), playing an important role in chemotaxis

COG id: COG1871

COG function: function code NT; Chemotaxis protein; stimulates methylation of MCP proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CheD family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHED_CLOB1 (A7FX01)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001384993.1
- ProteinModelPortal:   A7FX01
- SMR:   A7FX01
- STRING:   A7FX01
- GeneID:   5397206
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_2692
- eggNOG:   COG1871
- HOGENOM:   HBG531149
- OMA:   GGAAMFK
- ProtClustDB:   PRK13490
- BioCyc:   CBOT441770:CLB_2692-MONOMER
- GO:   GO:0006935
- HAMAP:   MF_01440
- InterPro:   IPR005659

Pfam domain/function: PF03975 CheD

EC number: =3.5.1.44

Molecular weight: Translated: 17318; Mature: 17318

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIKEIKVGIADLNVGKNPDKIITVGLGSCIGIALYDGIKCIGGLSHIMLPDSTQFSKVT
CCHHHEEEEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHC
NPMKFADLAIPILVEKMEKLGARKNGLKAKICGGASMFNFSDKSMVMDIGNRNGKAVKEK
CCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEECCCCCEEEEEECCCCCHHHHHH
LKELSIPLLAEDIGGNKGRTMIFDTSTGKVYIKTVGLGTKEI
HHHCCCCEEECCCCCCCCCEEEEECCCCEEEEEEECCCCCCC
>Mature Secondary Structure
MDIKEIKVGIADLNVGKNPDKIITVGLGSCIGIALYDGIKCIGGLSHIMLPDSTQFSKVT
CCHHHEEEEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHC
NPMKFADLAIPILVEKMEKLGARKNGLKAKICGGASMFNFSDKSMVMDIGNRNGKAVKEK
CCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEECCCCCEEEEEECCCCCHHHHHH
LKELSIPLLAEDIGGNKGRTMIFDTSTGKVYIKTVGLGTKEI
HHHCCCCEEECCCCCCCCCEEEEECCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA