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The map label for this gene is sigD [H]

Identifier: 148380599

GI number: 148380599

Start: 2793525

End: 2794253

Strand: Reverse

Name: sigD [H]

Synonym: CBO2641

Alternate gene names: 148380599

Gene position: 2794253-2793525 (Counterclockwise)

Preceding gene: 148380600

Following gene: 148380598

Centisome position: 71.89

GC content: 28.12

Gene sequence:

>729_bases
ATGGCAATAGCTGATAATAATTTATATGTTAAAGAAGAGATAGTAAAAAAATATATTCCTTTAGTTAAATATATAGCCTC
TAGGGTCATAATTGGTAAAACCAAATATATAGAATATGAGGACTTAGTTAGCTATGGTATGATCGGACTAATGGATGCTT
TAAATAAGTTTGATGAATCTAAAGGAATGAAATTTTCTACTTATGCATCAATAAGAATAAAGGGTTCTATGATAGACGAA
TTGAGAAGAAATAGCCCCATATCCAAAGGGGCTATGGATAAATTAAATAGATATAATGAAGCTATAGAGAAACTTCAAAA
GAAATTAAACAAAGAGCCAAACTTAATACAAATAGCAGATGAGTTAAACATATCTTTAAAAGAAGTAAGTGAAATTGAAA
ATTATATAAATTATATATCAGTAATTTCTCTAGAGGATTTAATATTTTCGTCAGAAGATGAGGTACCACTTATAGGAACT
ATAAAAGATGAAAAAAGTCCAAGCCCAGAAAAACATGTGGAAGAAAATGAACAGTTAGATTATTTAGCTAAAGCCATAGA
ACTTCTCAATGAAAAAGATAGGCTAGTAGTAACTCTGTATTATTATGAAGAACTAACTTTAAAGGAAATAGGAAAAATAT
TAAATGTATCTGAATCTAGAGTGTGTCAACTTCATAGCAGAGCTATAATCCATCTAAAGAAAGCTATGGCCAAGTTAAAA
TATAATTAA

Upstream 100 bases:

>100_bases
AAATTTTTAGACATAGATGTATTAACTAGAGAAAAAATAATAAAATTTATTTTTTCTAAAATGAGAAAACAAGTGCATAG
GCTATAGAGGAGGATAGGTC

Downstream 100 bases:

>100_bases
TAAACGGAATTAATAGGGAGAATGAAAGATGATAGCTTTTTTATTAATTATTATAGGAACAACACTTATATTTATAAATT
TTAAAGCTATAAAAAAAGAA

Product: RNA polymerase sigma factor for flagellar operon FliA

Products: NA

Alternate protein names: Sigma-28 [H]

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MAIADNNLYVKEEIVKKYIPLVKYIASRVIIGKTKYIEYEDLVSYGMIGLMDALNKFDESKGMKFSTYASIRIKGSMIDE
LRRNSPISKGAMDKLNRYNEAIEKLQKKLNKEPNLIQIADELNISLKEVSEIENYINYISVISLEDLIFSSEDEVPLIGT
IKDEKSPSPEKHVEENEQLDYLAKAIELLNEKDRLVVTLYYYEELTLKEIGKILNVSESRVCQLHSRAIIHLKKAMAKLK
YN

Sequences:

>Translated_242_residues
MAIADNNLYVKEEIVKKYIPLVKYIASRVIIGKTKYIEYEDLVSYGMIGLMDALNKFDESKGMKFSTYASIRIKGSMIDE
LRRNSPISKGAMDKLNRYNEAIEKLQKKLNKEPNLIQIADELNISLKEVSEIENYINYISVISLEDLIFSSEDEVPLIGT
IKDEKSPSPEKHVEENEQLDYLAKAIELLNEKDRLVVTLYYYEELTLKEIGKILNVSESRVCQLHSRAIIHLKKAMAKLK
YN
>Mature_241_residues
AIADNNLYVKEEIVKKYIPLVKYIASRVIIGKTKYIEYEDLVSYGMIGLMDALNKFDESKGMKFSTYASIRIKGSMIDEL
RRNSPISKGAMDKLNRYNEAIEKLQKKLNKEPNLIQIADELNISLKEVSEIENYINYISVISLEDLIFSSEDEVPLIGTI
KDEKSPSPEKHVEENEQLDYLAKAIELLNEKDRLVVTLYYYEELTLKEIGKILNVSESRVCQLHSRAIIHLKKAMAKLKY
N

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is required for the transcription of the flagellin and motility genes as well as for wild-

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=240, Percent_Identity=32.9166666666667, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1789098, Length=209, Percent_Identity=27.2727272727273, Blast_Score=77, Evalue=8e-16,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27906; Mature: 27775

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIADNNLYVKEEIVKKYIPLVKYIASRVIIGKTKYIEYEDLVSYGMIGLMDALNKFDES
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
KGMKFSTYASIRIKGSMIDELRRNSPISKGAMDKLNRYNEAIEKLQKKLNKEPNLIQIAD
CCCEEEEEEEEEECHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHH
ELNISLKEVSEIENYINYISVISLEDLIFSSEDEVPLIGTIKDEKSPSPEKHVEENEQLD
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHCCCHHHH
YLAKAIELLNEKDRLVVTLYYYEELTLKEIGKILNVSESRVCQLHSRAIIHLKKAMAKLK
HHHHHHHHHCCCCCEEEEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHC
YN
CC
>Mature Secondary Structure 
AIADNNLYVKEEIVKKYIPLVKYIASRVIIGKTKYIEYEDLVSYGMIGLMDALNKFDES
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
KGMKFSTYASIRIKGSMIDELRRNSPISKGAMDKLNRYNEAIEKLQKKLNKEPNLIQIAD
CCCEEEEEEEEEECHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHH
ELNISLKEVSEIENYINYISVISLEDLIFSSEDEVPLIGTIKDEKSPSPEKHVEENEQLD
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHCCCHHHH
YLAKAIELLNEKDRLVVTLYYYEELTLKEIGKILNVSESRVCQLHSRAIIHLKKAMAKLK
HHHHHHHHHCCCCCEEEEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHC
YN
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2832368; 9384377; 7602586; 8157612 [H]