| Definition | Clostridium botulinum A str. ATCC 3502, complete genome. |
|---|---|
| Accession | NC_009495 |
| Length | 3,886,916 |
Click here to switch to the map view.
The map label for this gene is nprM [H]
Identifier: 148379414
GI number: 148379414
Start: 1569042
End: 1570769
Strand: Reverse
Name: nprM [H]
Synonym: CBO1442
Alternate gene names: 148379414
Gene position: 1570769-1569042 (Counterclockwise)
Preceding gene: 148379415
Following gene: 148379411
Centisome position: 40.41
GC content: 28.59
Gene sequence:
>1728_bases ATGAAAAGTAAAAAATTATTAACTATACTTTTAAGTGCTATAATAACAGCTTCATCAATCTCATCAGTTTACGCTGCAGA ATCCGTGGGGATAAAAAGTAAATACCAACCAAAAACTACTACTATATTTTGGGAAAAAGGTAAACAAAATAATAAAAAAT CTGCCACTAATATAGCTTCAGAACAATTCAACAATTTCGAAGAAATAAATCAGTTCTTTCAACAAAATATATCTAGGTTT GGTTTAAAAAAAGGCTCTCTTAAAAGTACCAAAGCTTTAAAGGATGAAAAAGGGAAAACCCACTATCATACAATTTATCA AGTGGAGGGTATACCTGTATACTATGGAAGAATTGTTTTTACTACTGAAAAAGATTCCACTATAAGCTCTATAAATGGTG GAGTAGACATTTCCTTTGAAAATGAGAATTGGAAAAACAAAATTAAACTATCAAAAAGTGATGCTATAGCAAAAGCTAAA AATAACATCAAATATGAAGAGTTACATGATTCAAAGGCAGACTTATATTTATATAATTTTGAAGAAAAGCCTTATGTTGT TTATTTAATAGATTTAAGCACAGATACTGGAGATTGGAATGTTTTTGTTAATGCTGAAGATGGTTCTATAGTAAATAAAT TTAATAACATCCCTACTCTAACTAATACTAGAGATAAGAAATTCACCAGTACTAAAAAAACTAATACTAAAGTTAATAAA TCAAATAATTTAATTGATGTTCAAGGTAATACTATTAAAGGAAAAGGTAAAAGCTCTTTAAATGGAATAGTAGATATTGA TCTAACTTATAAAGATGGAAAATATTATTTAAAAAATAGCAATAAAAATATTTATGTCTATGATCTAAATAATAAATATA TAAACACTTTTACTACCCCTAAATCTTCTATATTAAAGGCTTCTAAGCTTGTAGAAAATAATAATAACGAATTTATAGAT GATAAACATATAATTGCTGTAGATGCTTATATAAACTTAGAGAAGACTTATGATTATTACAAAAATAAATTTAATAGAAA TAGTATTGATAATAAAGGTATGAACGTTGAAGCCTTTATTCATCATGGTGAGAAATATGCGGGTGCTGAGTGGAGCGAAA ATCTTGGTTCCATGCTTTTAGGGGATGGTGATGGAAGAAATAGTTCTCATATGTCAAAGGCTTTAGATGTTGTAGGTCAT GAGTTTAGTCATGGTGTAACACGTAAAGAATCTAATCTTAAATATGAAAATGAATCCGGAGCTTTAAATGAATCCTTTTC AGATATTATGGGAATAGCCATTAAAGGTAAAAATTTCAAATTAGGTGAAGACTGCTGGACACCAGATATTGAGGGGGATG CAATAAGAGATATGCAAGATCCATCTAAAGGATACCAGCCAGCTCATATGAAAGATTATAGATCTATGGATATACGCTAT GACAATGGTGGTGTTCATGTAAATTCAGGTATAATAAATCACGCAGCTTACTTGATTGCAGATGGTATAGAAAAATTAGG AGTAGAAAATAGTAAAGATATTATGGCAAAATTATTTTATACAGCTAATTGCTATGAATGGGATGAGACAACAAATTTCT CTAAATGTAGAAATGATTTAATTAAAGTTACTAAAGACCTTTACGGTGAAAATAGTAAATATGTTCAAATTGTAGAAAAT GCTTTTGACAAAGTTGGAATAACTGCTACACCTCAATTACCATTATAA
Upstream 100 bases:
>100_bases ACTATATAAAAAATTAGATTTAAATAAATAAGATGATTTCTATTCAAGTAAGAAATCATCTTATTTATGTATACTAATTA TATTGGGGGTTAAGATATTT
Downstream 100 bases:
>100_bases TAAAATTCTATTAAACCTATAAATATAACTATGTAAAAAACTAAATTTAAATAAATAAGATGATTTCTTATTTGCATAGA AATCATCTTATTTATATATA
Product: secreted protease
Products: NA
Alternate protein names: Neutral protease [H]
Number of amino acids: Translated: 575; Mature: 575
Protein sequence:
>575_residues MKSKKLLTILLSAIITASSISSVYAAESVGIKSKYQPKTTTIFWEKGKQNNKKSATNIASEQFNNFEEINQFFQQNISRF GLKKGSLKSTKALKDEKGKTHYHTIYQVEGIPVYYGRIVFTTEKDSTISSINGGVDISFENENWKNKIKLSKSDAIAKAK NNIKYEELHDSKADLYLYNFEEKPYVVYLIDLSTDTGDWNVFVNAEDGSIVNKFNNIPTLTNTRDKKFTSTKKTNTKVNK SNNLIDVQGNTIKGKGKSSLNGIVDIDLTYKDGKYYLKNSNKNIYVYDLNNKYINTFTTPKSSILKASKLVENNNNEFID DKHIIAVDAYINLEKTYDYYKNKFNRNSIDNKGMNVEAFIHHGEKYAGAEWSENLGSMLLGDGDGRNSSHMSKALDVVGH EFSHGVTRKESNLKYENESGALNESFSDIMGIAIKGKNFKLGEDCWTPDIEGDAIRDMQDPSKGYQPAHMKDYRSMDIRY DNGGVHVNSGIINHAAYLIADGIEKLGVENSKDIMAKLFYTANCYEWDETTNFSKCRNDLIKVTKDLYGENSKYVQIVEN AFDKVGITATPQLPL
Sequences:
>Translated_575_residues MKSKKLLTILLSAIITASSISSVYAAESVGIKSKYQPKTTTIFWEKGKQNNKKSATNIASEQFNNFEEINQFFQQNISRF GLKKGSLKSTKALKDEKGKTHYHTIYQVEGIPVYYGRIVFTTEKDSTISSINGGVDISFENENWKNKIKLSKSDAIAKAK NNIKYEELHDSKADLYLYNFEEKPYVVYLIDLSTDTGDWNVFVNAEDGSIVNKFNNIPTLTNTRDKKFTSTKKTNTKVNK SNNLIDVQGNTIKGKGKSSLNGIVDIDLTYKDGKYYLKNSNKNIYVYDLNNKYINTFTTPKSSILKASKLVENNNNEFID DKHIIAVDAYINLEKTYDYYKNKFNRNSIDNKGMNVEAFIHHGEKYAGAEWSENLGSMLLGDGDGRNSSHMSKALDVVGH EFSHGVTRKESNLKYENESGALNESFSDIMGIAIKGKNFKLGEDCWTPDIEGDAIRDMQDPSKGYQPAHMKDYRSMDIRY DNGGVHVNSGIINHAAYLIADGIEKLGVENSKDIMAKLFYTANCYEWDETTNFSKCRNDLIKVTKDLYGENSKYVQIVEN AFDKVGITATPQLPL >Mature_575_residues MKSKKLLTILLSAIITASSISSVYAAESVGIKSKYQPKTTTIFWEKGKQNNKKSATNIASEQFNNFEEINQFFQQNISRF GLKKGSLKSTKALKDEKGKTHYHTIYQVEGIPVYYGRIVFTTEKDSTISSINGGVDISFENENWKNKIKLSKSDAIAKAK NNIKYEELHDSKADLYLYNFEEKPYVVYLIDLSTDTGDWNVFVNAEDGSIVNKFNNIPTLTNTRDKKFTSTKKTNTKVNK SNNLIDVQGNTIKGKGKSSLNGIVDIDLTYKDGKYYLKNSNKNIYVYDLNNKYINTFTTPKSSILKASKLVENNNNEFID DKHIIAVDAYINLEKTYDYYKNKFNRNSIDNKGMNVEAFIHHGEKYAGAEWSENLGSMLLGDGDGRNSSHMSKALDVVGH EFSHGVTRKESNLKYENESGALNESFSDIMGIAIKGKNFKLGEDCWTPDIEGDAIRDMQDPSKGYQPAHMKDYRSMDIRY DNGGVHVNSGIINHAAYLIADGIEKLGVENSKDIMAKLFYTANCYEWDETTNFSKCRNDLIKVTKDLYGENSKYVQIVEN AFDKVGITATPQLPL
Specific function: Extracellular zinc metalloprotease [H]
COG id: COG3227
COG function: function code E; Zinc metalloprotease (elastase)
Gene ontology:
Cell location: Secreted [H]
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Belongs to the peptidase M4 family [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR005075 - InterPro: IPR013856 - InterPro: IPR001570 - InterPro: IPR011096 [H]
Pfam domain/function: PF07504 FTP; PF03413 PepSY; PF01447 Peptidase_M4; PF02868 Peptidase_M4_C [H]
EC number: =3.4.24.28 [H]
Molecular weight: Translated: 64950; Mature: 64950
Theoretical pI: Translated: 8.52; Mature: 8.52
Prosite motif: PS00142 ZINC_PROTEASE
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.5 %Cys (Translated Protein) 1.6 %Met (Translated Protein) 2.1 %Cys+Met (Translated Protein) 0.5 %Cys (Mature Protein) 1.6 %Met (Mature Protein) 2.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKSKKLLTILLSAIITASSISSVYAAESVGIKSKYQPKTTTIFWEKGKQNNKKSATNIAS CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHH EQFNNFEEINQFFQQNISRFGLKKGSLKSTKALKDEKGKTHYHTIYQVEGIPVYYGRIVF HHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCEEEEEEEECCEEEEEEEEEE TTEKDSTISSINGGVDISFENENWKNKIKLSKSDAIAKAKNNIKYEELHDSKADLYLYNF EECCCCCHHHCCCCEEEEECCCCCCCEEEECCHHHHHHHHCCCCHHHHCCCCCCEEEEEE EEKPYVVYLIDLSTDTGDWNVFVNAEDGSIVNKFNNIPTLTNTRDKKFTSTKKTNTKVNK CCCCEEEEEEEECCCCCCEEEEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCEECC SNNLIDVQGNTIKGKGKSSLNGIVDIDLTYKDGKYYLKNSNKNIYVYDLNNKYINTFTTP CCCEEEECCCEEECCCCCCCCCEEEEEEEEECCEEEEECCCCEEEEEECCCCEEEEECCC KSSILKASKLVENNNNEFIDDKHIIAVDAYINLEKTYDYYKNKFNRNSIDNKGMNVEAFI HHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEHHHHHHHHCCCCCCCCCCCCCEEEEEE HHGEKYAGAEWSENLGSMLLGDGDGRNSSHMSKALDVVGHEFSHGVTRKESNLKYENESG ECCCCCCCCCHHHCCCCEEEECCCCCCHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCC ALNESFSDIMGIAIKGKNFKLGEDCWTPDIEGDAIRDMQDPSKGYQPAHMKDYRSMDIRY CCCCCHHHHEEEEEECCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHCCCCEEEEE DNGGVHVNSGIINHAAYLIADGIEKLGVENSKDIMAKLFYTANCYEWDETTNFSKCRNDL CCCCEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHEEECCEEECCCCCCHHHHHHHH IKVTKDLYGENSKYVQIVENAFDKVGITATPQLPL HHHHHHHHCCCCCEEHEEHHHHHHCCCEECCCCCC >Mature Secondary Structure MKSKKLLTILLSAIITASSISSVYAAESVGIKSKYQPKTTTIFWEKGKQNNKKSATNIAS CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHH EQFNNFEEINQFFQQNISRFGLKKGSLKSTKALKDEKGKTHYHTIYQVEGIPVYYGRIVF HHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCEEEEEEEECCEEEEEEEEEE TTEKDSTISSINGGVDISFENENWKNKIKLSKSDAIAKAKNNIKYEELHDSKADLYLYNF EECCCCCHHHCCCCEEEEECCCCCCCEEEECCHHHHHHHHCCCCHHHHCCCCCCEEEEEE EEKPYVVYLIDLSTDTGDWNVFVNAEDGSIVNKFNNIPTLTNTRDKKFTSTKKTNTKVNK CCCCEEEEEEEECCCCCCEEEEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCEECC SNNLIDVQGNTIKGKGKSSLNGIVDIDLTYKDGKYYLKNSNKNIYVYDLNNKYINTFTTP CCCEEEECCCEEECCCCCCCCCEEEEEEEEECCEEEEECCCCEEEEEECCCCEEEEECCC KSSILKASKLVENNNNEFIDDKHIIAVDAYINLEKTYDYYKNKFNRNSIDNKGMNVEAFI HHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEHHHHHHHHCCCCCCCCCCCCCEEEEEE HHGEKYAGAEWSENLGSMLLGDGDGRNSSHMSKALDVVGHEFSHGVTRKESNLKYENESG ECCCCCCCCCHHHCCCCEEEECCCCCCHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCC ALNESFSDIMGIAIKGKNFKLGEDCWTPDIEGDAIRDMQDPSKGYQPAHMKDYRSMDIRY CCCCCHHHHEEEEEECCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHCCCCEEEEE DNGGVHVNSGIINHAAYLIADGIEKLGVENSKDIMAKLFYTANCYEWDETTNFSKCRNDL CCCCEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHEEECCEEECCCCCCHHHHHHHH IKVTKDLYGENSKYVQIVENAFDKVGITATPQLPL HHHHHHHHCCCCCEEHEEHHHHHHCCCEECCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 7764969 [H]