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The map label for this gene is thiJ [C]

Identifier: 148379015

GI number: 148379015

Start: 1126794

End: 1127378

Strand: Reverse

Name: thiJ [C]

Synonym: CBO1025

Alternate gene names: 148379015

Gene position: 1127378-1126794 (Counterclockwise)

Preceding gene: 148379022

Following gene: 148379013

Centisome position: 29.0

GC content: 33.5

Gene sequence:

>585_bases
ATGAAAAAAGTATTATTACTTCTTGCCAATGGTTTTGAGGCTGTAGAAGCAAGTGTTTTTACAGATGTTATAGGATGGAA
CAAATTAGAAGGTGATGGTACCACAGAATTAATAACTGTTGGAATAAGAGAAAAATTAAAATGTACTTTTAACTTCACAG
TAACTCCTGAAATGCATGTCAGTGAAGTTAATATTGATGAATTTGATGCACTTGCAATACCAGGTGGATTTGAAGAAGCA
GGATTTTATGAAGATGCTTATAGCGAAGATTTTTTAAACATAATCAGAGAATTTGACAAAGCAAGAAAAACAATAGCTTC
TATTTGCGTTGGTGCGCTGCCTATTGGGAAAAGTGGAGTACTAGTTAATAGAAATGCCACAACTTACAATTTAGGAAATG
GTAGAAGGCAAAAACAATTATCTGAATTTGGTGCAAATGTTCTTCGTGATGAACCTGTTGTTGTAGATAAAAATATAATC
ACTTCCTACAATCCTTCAACTGCTTTCCATGTAGCTTTCAAACTTTTAGAGCTGCTCACCTCTAAAGAAAACTGTGTTAA
TGTTAAGAGACTAATGGGGTTTTAG

Upstream 100 bases:

>100_bases
ATTATTAATTTCATTTATTTCAATTTTACTGATAGCACATATAGCATTATACTTAAGAAAAACAAATCTAAAATATATAA
TTAAAAGGAGATATCAAAGT

Downstream 100 bases:

>100_bases
CAAAATAATAAAGTACCAAATTTACTATATTAGAACAAAAGCAATATTCTTAAGAGACGTAACAAGGACTTACTTTTTTA
TGGTAGGTCCTATTTTATTA

Product: DJ-1/PfpI family protein

Products: NA

Alternate protein names: Intracellular Protease/Amidase; DJ-1/PfpI Family Protein; Protein ThiJ; ThiJ/PfpI Family Protein; Protease; ThiJ/PfpI Domain Protein; ThiJ/PfpI Domain-Containing Protein; Thij/Pfpi DomaiN-Containing Protein; Protease I; Protease DJ-1/PfpI Family; Metal Dependent Phosphohydrolase; Dj-1/Pfpi Family

Number of amino acids: Translated: 194; Mature: 194

Protein sequence:

>194_residues
MKKVLLLLANGFEAVEASVFTDVIGWNKLEGDGTTELITVGIREKLKCTFNFTVTPEMHVSEVNIDEFDALAIPGGFEEA
GFYEDAYSEDFLNIIREFDKARKTIASICVGALPIGKSGVLVNRNATTYNLGNGRRQKQLSEFGANVLRDEPVVVDKNII
TSYNPSTAFHVAFKLLELLTSKENCVNVKRLMGF

Sequences:

>Translated_194_residues
MKKVLLLLANGFEAVEASVFTDVIGWNKLEGDGTTELITVGIREKLKCTFNFTVTPEMHVSEVNIDEFDALAIPGGFEEA
GFYEDAYSEDFLNIIREFDKARKTIASICVGALPIGKSGVLVNRNATTYNLGNGRRQKQLSEFGANVLRDEPVVVDKNII
TSYNPSTAFHVAFKLLELLTSKENCVNVKRLMGF
>Mature_194_residues
MKKVLLLLANGFEAVEASVFTDVIGWNKLEGDGTTELITVGIREKLKCTFNFTVTPEMHVSEVNIDEFDALAIPGGFEEA
GFYEDAYSEDFLNIIREFDKARKTIASICVGALPIGKSGVLVNRNATTYNLGNGRRQKQLSEFGANVLRDEPVVVDKNII
TSYNPSTAFHVAFKLLELLTSKENCVNVKRLMGF

Specific function: Thiamine biosynthesis [C]

COG id: COG0693

COG function: function code R; Putative intracellular protease/amidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Drosophila melanogaster, GI28571932, Length=193, Percent_Identity=26.9430051813472, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24653499, Length=195, Percent_Identity=29.2307692307692, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 21475; Mature: 21475

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVLLLLANGFEAVEASVFTDVIGWNKLEGDGTTELITVGIREKLKCTFNFTVTPEMHV
CCEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCEEEEEEEEEECCCCEE
SEVNIDEFDALAIPGGFEEAGFYEDAYSEDFLNIIREFDKARKTIASICVGALPIGKSGV
EECCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
LVNRNATTYNLGNGRRQKQLSEFGANVLRDEPVVVDKNIITSYNPSTAFHVAFKLLELLT
EEECCCCEEECCCCHHHHHHHHHHHHHHCCCCEEEECCEEECCCCCHHHHHHHHHHHHHH
SKENCVNVKRLMGF
CCCHHHHHHHHHCC
>Mature Secondary Structure
MKKVLLLLANGFEAVEASVFTDVIGWNKLEGDGTTELITVGIREKLKCTFNFTVTPEMHV
CCEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCEEEEEEEEEECCCCEE
SEVNIDEFDALAIPGGFEEAGFYEDAYSEDFLNIIREFDKARKTIASICVGALPIGKSGV
EECCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
LVNRNATTYNLGNGRRQKQLSEFGANVLRDEPVVVDKNIITSYNPSTAFHVAFKLLELLT
EEECCCCEEECCCCHHHHHHHHHHHHHHCCCCEEEECCEEECCCCCHHHHHHHHHHHHHH
SKENCVNVKRLMGF
CCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA