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The map label for this gene is trmD [H]

Identifier: 148360986

GI number: 148360986

Start: 490942

End: 491694

Strand: Reverse

Name: trmD [H]

Synonym: LPC_2946

Alternate gene names: 148360986

Gene position: 491694-490942 (Counterclockwise)

Preceding gene: 148360985

Following gene: 148360987

Centisome position: 13.75

GC content: 42.63

Gene sequence:

>753_bases
GTGGCATTACATCTTGGAGTCATCACCCTGTTGCCTGAAATAATACAAGGCATCCATTATGGGGTAACCGGCCGAGCTAT
TGAACAAGGCTTGGTTAAAATTGATTGCTGGAATCCCAGGGATTGGTCATCAAGACCTTATAAGCAAGTGGATGACAAGC
CCTATGGTGGTGGACCAGGAATGGTCATGATGTATGAGCCATTGCATGCTGCAATAAAGCATGCGCACAGTGAAATGAAG
GAAAACTGCAAAACAATTTACTTAAGCCCTCAAGGCAAGGTAGTTAGGCAGAACGATTTAAAACAAATAGCTACCCAAAA
ACAATCCTTGCTATTTGTTGCAGGGAGATATGAAGGAATTGATGAGCGAATCATTAATCACCACATAGATGAAGAATGGT
CGCTAGGGGATTTTGTATTAAGTGGCGGTGAATTGGCCGCAATGGTATTTATTGACGCGATAATTCGCTTGATACCTGGT
AGCCTTGGGCATTTGGGTTCAGCAGAACAAGATTCATTTATGAATGGATTACTGGACTGCCCACATTATACTCGGCCAGC
AACTATAAATGGACTGGGTGTACCTGATGTTTTATTAGGAGGAAACCATAAAGAAATAGAACGCTGGCGTAGGAAACAAT
CCCTGGGTAAAACCTGGCTCAAACGCCCGGATTTACTTGAGAAAATACAATTAAGTGAAACAGACAAGCAATTGCTTGCT
GAGTTTAAATGCGAACACGGCGATTCCTGTTGA

Upstream 100 bases:

>100_bases
GTGAAAAAAGACATTTAATTCCCTATCTTCCAGGACAATTTGTAATCAATATTGATGAAAGCCAGCAAGTAATCACTGTT
GATTGGGATATGAATTTTTA

Downstream 100 bases:

>100_bases
GGATAAGCCAATTTGAGGAGTAGTCTATGACTAATATTATTGACCAAATTAATGCCGAACAAATGCAAGGCAAAGAAATT
CCTGATTTCAACCCAGGCGA

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MALHLGVITLLPEIIQGIHYGVTGRAIEQGLVKIDCWNPRDWSSRPYKQVDDKPYGGGPGMVMMYEPLHAAIKHAHSEMK
ENCKTIYLSPQGKVVRQNDLKQIATQKQSLLFVAGRYEGIDERIINHHIDEEWSLGDFVLSGGELAAMVFIDAIIRLIPG
SLGHLGSAEQDSFMNGLLDCPHYTRPATINGLGVPDVLLGGNHKEIERWRRKQSLGKTWLKRPDLLEKIQLSETDKQLLA
EFKCEHGDSC

Sequences:

>Translated_250_residues
MALHLGVITLLPEIIQGIHYGVTGRAIEQGLVKIDCWNPRDWSSRPYKQVDDKPYGGGPGMVMMYEPLHAAIKHAHSEMK
ENCKTIYLSPQGKVVRQNDLKQIATQKQSLLFVAGRYEGIDERIINHHIDEEWSLGDFVLSGGELAAMVFIDAIIRLIPG
SLGHLGSAEQDSFMNGLLDCPHYTRPATINGLGVPDVLLGGNHKEIERWRRKQSLGKTWLKRPDLLEKIQLSETDKQLLA
EFKCEHGDSC
>Mature_249_residues
ALHLGVITLLPEIIQGIHYGVTGRAIEQGLVKIDCWNPRDWSSRPYKQVDDKPYGGGPGMVMMYEPLHAAIKHAHSEMKE
NCKTIYLSPQGKVVRQNDLKQIATQKQSLLFVAGRYEGIDERIINHHIDEEWSLGDFVLSGGELAAMVFIDAIIRLIPGS
LGHLGSAEQDSFMNGLLDCPHYTRPATINGLGVPDVLLGGNHKEIERWRRKQSLGKTWLKRPDLLEKIQLSETDKQLLAE
FKCEHGDSC

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=244, Percent_Identity=58.1967213114754, Blast_Score=289, Evalue=1e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 28031; Mature: 27899

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALHLGVITLLPEIIQGIHYGVTGRAIEQGLVKIDCWNPRDWSSRPYKQVDDKPYGGGPG
CCEEHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCC
MVMMYEPLHAAIKHAHSEMKENCKTIYLSPQGKVVRQNDLKQIATQKQSLLFVAGRYEGI
EEEEEHHHHHHHHHHHHHHHCCCCEEEECCCCCEECHHHHHHHHHHHHHEEEEECCCCCH
DERIINHHIDEEWSLGDFVLSGGELAAMVFIDAIIRLIPGSLGHLGSAEQDSFMNGLLDC
HHHHHHHCCCCCCCCHHHEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCC
PHYTRPATINGLGVPDVLLGGNHKEIERWRRKQSLGKTWLKRPDLLEKIQLSETDKQLLA
CCCCCCCCCCCCCCCHHEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHH
EFKCEHGDSC
HHHHCCCCCC
>Mature Secondary Structure 
ALHLGVITLLPEIIQGIHYGVTGRAIEQGLVKIDCWNPRDWSSRPYKQVDDKPYGGGPG
CEEHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCC
MVMMYEPLHAAIKHAHSEMKENCKTIYLSPQGKVVRQNDLKQIATQKQSLLFVAGRYEGI
EEEEEHHHHHHHHHHHHHHHCCCCEEEECCCCCEECHHHHHHHHHHHHHEEEEECCCCCH
DERIINHHIDEEWSLGDFVLSGGELAAMVFIDAIIRLIPGSLGHLGSAEQDSFMNGLLDC
HHHHHHHCCCCCCCCHHHEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCC
PHYTRPATINGLGVPDVLLGGNHKEIERWRRKQSLGKTWLKRPDLLEKIQLSETDKQLLA
CCCCCCCCCCCCCCCHHEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHH
EFKCEHGDSC
HHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA