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The map label for this gene is rimO [H]

Identifier: 148360663

GI number: 148360663

Start: 845792

End: 847111

Strand: Reverse

Name: rimO [H]

Synonym: LPC_2609

Alternate gene names: 148360663

Gene position: 847111-845792 (Counterclockwise)

Preceding gene: 148360662

Following gene: 148360669

Centisome position: 23.69

GC content: 41.21

Gene sequence:

>1320_bases
ATGAATCATAAAGTAGGATTTGTAAGTTTAGGATGCCCCAAAGCACTGGTCGATTCAGAGCGAATCATTACTCAGCTTAA
AGCACAAGGCTATGAATTGGTTCCCACTTATGAGGATGCTGGAGTCGTCGTAATTAATACGTGCGGCTTCATTGACAGCG
CTGTCCAGGAATCACTGGATACAATTAAAGAAGCTATGGCTGAAAACGGGCGAGTAATCGTTACAGGTTGTTTAGGAGCT
AAAGCGGACGTAATTAAAAATGCCTGTCCTGATGTCCTGCATATCAGTGGTGCTCATGCCTACGAAGAAGTAGTCAATGC
GGTACACCAACACTTGCCGCCTCCTGCAGACCCATTTACACAACTGATTCCCCCACAGGGAATAAAACTTACACCTCGGC
ATTATGCTTATTTAAAAATATCAGAAGGATGCAACCAAAAATGCACTTTTTGCATTATCCCTACGATGAGAGGAAAATTG
CAAAGCTATCCAATGGCCCAAATTCTTACTGAAGCTAAGAAATTAAAACAAGCCGGAGTTAAAGAATTGCTGGTTATCTC
ACAAGACACCAGCGCTTATGGCGTGGATACACGTTATCAGCAGGTCGAATGGCAAGGAAAAACAGTCAATACCCGTTTTT
ACGATCTATGCGAACAATTAGGCGAATTAGGAATTTGGGTGAGACTCCATTATGTCTATCCCTATCCCCATGTTGATGAT
ATTGTTCCTCTAATGCGCGATGGCCTGATTTTGCCTTACCTGGATATACCATTGCAACATGCCAATTCACGCATATTAAA
AGCAATGAAAAGACCCGCCAGCAGTGAAAATACCTTATTGCGGATTGCTTCATGGAGAGAAATTTGCCCTGACATTACGT
TACGCTCCACATTCATTGTAGGTTTCCCTGGTGAAACAGAAGAGGAGTTTTCCGAATTACTAGCCTTCCTTAAAGAGGCA
CAACTAGACAGAGTCGGTTGTTTCAAATACTCCCCTGTTGAAGGAGCCAAAGCAAATGATTTGGATAATCCTGTATCGGA
GGATATTAAAGAAGAGCGTTATCATCGTTTTATGCAAGTACAAGCAGAAATTAGCCGTAATAAATTAAAAAATAAAATTG
GCAGTACTCAAACTGTTTTAATTGATGAGATAACCGAAGATCAAATCATAGCTCGTAGTAAAAGCGATGCTCCTGAAATC
GATGGTCTTGTTTATTTGCCAAAAATATCCGGGATTACTGTTGGGTCTTTCGCAGAAGCAATGATAACCGACAGTGACGA
CTATGATTTGTATGGTGATTTGGAATACTCTCTTGCATGA

Upstream 100 bases:

>100_bases
ATGAGAACTAAAGTAACTATGGGCGCTTTTGTATGAAATCAGTCGACACTGGAACGATTAAGCAGTATAATTAGCTCATT
TTTTTTACAGATAGAAGTTT

Downstream 100 bases:

>100_bases
AAGAAATGGCATTAAAACAGTACTTTTTCTGCTCACCTTCATTGGCAAGAGAGAGAATATTTATTGTATCTAGCGTACAA
AATGCCTTTTAAATCGCCAT

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MNHKVGFVSLGCPKALVDSERIITQLKAQGYELVPTYEDAGVVVINTCGFIDSAVQESLDTIKEAMAENGRVIVTGCLGA
KADVIKNACPDVLHISGAHAYEEVVNAVHQHLPPPADPFTQLIPPQGIKLTPRHYAYLKISEGCNQKCTFCIIPTMRGKL
QSYPMAQILTEAKKLKQAGVKELLVISQDTSAYGVDTRYQQVEWQGKTVNTRFYDLCEQLGELGIWVRLHYVYPYPHVDD
IVPLMRDGLILPYLDIPLQHANSRILKAMKRPASSENTLLRIASWREICPDITLRSTFIVGFPGETEEEFSELLAFLKEA
QLDRVGCFKYSPVEGAKANDLDNPVSEDIKEERYHRFMQVQAEISRNKLKNKIGSTQTVLIDEITEDQIIARSKSDAPEI
DGLVYLPKISGITVGSFAEAMITDSDDYDLYGDLEYSLA

Sequences:

>Translated_439_residues
MNHKVGFVSLGCPKALVDSERIITQLKAQGYELVPTYEDAGVVVINTCGFIDSAVQESLDTIKEAMAENGRVIVTGCLGA
KADVIKNACPDVLHISGAHAYEEVVNAVHQHLPPPADPFTQLIPPQGIKLTPRHYAYLKISEGCNQKCTFCIIPTMRGKL
QSYPMAQILTEAKKLKQAGVKELLVISQDTSAYGVDTRYQQVEWQGKTVNTRFYDLCEQLGELGIWVRLHYVYPYPHVDD
IVPLMRDGLILPYLDIPLQHANSRILKAMKRPASSENTLLRIASWREICPDITLRSTFIVGFPGETEEEFSELLAFLKEA
QLDRVGCFKYSPVEGAKANDLDNPVSEDIKEERYHRFMQVQAEISRNKLKNKIGSTQTVLIDEITEDQIIARSKSDAPEI
DGLVYLPKISGITVGSFAEAMITDSDDYDLYGDLEYSLA
>Mature_439_residues
MNHKVGFVSLGCPKALVDSERIITQLKAQGYELVPTYEDAGVVVINTCGFIDSAVQESLDTIKEAMAENGRVIVTGCLGA
KADVIKNACPDVLHISGAHAYEEVVNAVHQHLPPPADPFTQLIPPQGIKLTPRHYAYLKISEGCNQKCTFCIIPTMRGKL
QSYPMAQILTEAKKLKQAGVKELLVISQDTSAYGVDTRYQQVEWQGKTVNTRFYDLCEQLGELGIWVRLHYVYPYPHVDD
IVPLMRDGLILPYLDIPLQHANSRILKAMKRPASSENTLLRIASWREICPDITLRSTFIVGFPGETEEEFSELLAFLKEA
QLDRVGCFKYSPVEGAKANDLDNPVSEDIKEERYHRFMQVQAEISRNKLKNKIGSTQTVLIDEITEDQIIARSKSDAPEI
DGLVYLPKISGITVGSFAEAMITDSDDYDLYGDLEYSLA

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=421, Percent_Identity=26.3657957244656, Blast_Score=142, Evalue=7e-34,
Organism=Homo sapiens, GI28872782, Length=421, Percent_Identity=26.3657957244656, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI93277076, Length=402, Percent_Identity=26.6169154228856, Blast_Score=136, Evalue=4e-32,
Organism=Escherichia coli, GI1787057, Length=430, Percent_Identity=63.2558139534884, Blast_Score=581, Evalue=1e-167,
Organism=Escherichia coli, GI1786882, Length=451, Percent_Identity=28.159645232816, Blast_Score=148, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI17553146, Length=474, Percent_Identity=23.8396624472574, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71996771, Length=263, Percent_Identity=27.7566539923954, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21356207, Length=481, Percent_Identity=27.027027027027, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI19922432, Length=454, Percent_Identity=23.7885462555066, Blast_Score=117, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 48989; Mature: 48989

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS50926 TRAM ; PS00435 PEROXIDASE_1 ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHKVGFVSLGCPKALVDSERIITQLKAQGYELVPTYEDAGVVVINTCGFIDSAVQESLD
CCCCEEEEEECCCHHHHHHHHHHHHHHHCCEEEEECCCCCCEEEEECCCHHHHHHHHHHH
TIKEAMAENGRVIVTGCLGAKADVIKNACPDVLHISGAHAYEEVVNAVHQHLPPPADPFT
HHHHHHHCCCCEEEEECCCCHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHCCCCCCCHH
QLIPPQGIKLTPRHYAYLKISEGCNQKCTFCIIPTMRGKLQSYPMAQILTEAKKLKQAGV
HCCCCCCCEECCCEEEEEEECCCCCCCCEEEEECCCCCHHHCCCHHHHHHHHHHHHHCCH
KELLVISQDTSAYGVDTRYQQVEWQGKTVNTRFYDLCEQLGELGIWVRLHYVYPYPHVDD
HEEEEEECCCCCCCCCCCHHEEEECCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCHHH
IVPLMRDGLILPYLDIPLQHANSRILKAMKRPASSENTLLRIASWREICPDITLRSTFIV
HHHHHHCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHCCCCEEEEEEEE
GFPGETEEEFSELLAFLKEAQLDRVGCFKYSPVEGAKANDLDNPVSEDIKEERYHRFMQV
ECCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
QAEISRNKLKNKIGSTQTVLIDEITEDQIIARSKSDAPEIDGLVYLPKISGITVGSFAEA
HHHHHHHHHHHHHCCCEEEEEECCCCCHHHHCCCCCCCCCCCEEEECCCCCEEHHHHHHH
MITDSDDYDLYGDLEYSLA
HCCCCCCCCEECCEEEECC
>Mature Secondary Structure
MNHKVGFVSLGCPKALVDSERIITQLKAQGYELVPTYEDAGVVVINTCGFIDSAVQESLD
CCCCEEEEEECCCHHHHHHHHHHHHHHHCCEEEEECCCCCCEEEEECCCHHHHHHHHHHH
TIKEAMAENGRVIVTGCLGAKADVIKNACPDVLHISGAHAYEEVVNAVHQHLPPPADPFT
HHHHHHHCCCCEEEEECCCCHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHCCCCCCCHH
QLIPPQGIKLTPRHYAYLKISEGCNQKCTFCIIPTMRGKLQSYPMAQILTEAKKLKQAGV
HCCCCCCCEECCCEEEEEEECCCCCCCCEEEEECCCCCHHHCCCHHHHHHHHHHHHHCCH
KELLVISQDTSAYGVDTRYQQVEWQGKTVNTRFYDLCEQLGELGIWVRLHYVYPYPHVDD
HEEEEEECCCCCCCCCCCHHEEEECCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCHHH
IVPLMRDGLILPYLDIPLQHANSRILKAMKRPASSENTLLRIASWREICPDITLRSTFIV
HHHHHHCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHCCCCEEEEEEEE
GFPGETEEEFSELLAFLKEAQLDRVGCFKYSPVEGAKANDLDNPVSEDIKEERYHRFMQV
ECCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
QAEISRNKLKNKIGSTQTVLIDEITEDQIIARSKSDAPEIDGLVYLPKISGITVGSFAEA
HHHHHHHHHHHHHCCCEEEEEECCCCCHHHHCCCCCCCCCCCEEEECCCCCEEHHHHHHH
MITDSDDYDLYGDLEYSLA
HCCCCCCCCEECCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA