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The map label for this gene is pcm [H]

Identifier: 148360648

GI number: 148360648

Start: 874011

End: 874661

Strand: Reverse

Name: pcm [H]

Synonym: LPC_2594

Alternate gene names: 148360648

Gene position: 874661-874011 (Counterclockwise)

Preceding gene: 148360641

Following gene: 148360649

Centisome position: 24.46

GC content: 37.48

Gene sequence:

>651_bases
ATGAATCACAGCGCACGCATAAATATGATCAAACAACAGTTACGAACTGGTGATGTATTAAACGAATCCATCCTTGACTT
ATACGATGAGATCTTAAGACATGAGTTTGTTCCTGAACCTTTTTCTCATTTTGCTTATTCAGACATGCAAATTCCTTTGG
CACACGGTCAAAGGATGTTAACTCCATTGGAAGAAGGTACAATTTTACAATCCCTTGATCTTAAAGGCCATGAAACCGTG
TTGGAAGTGGGAACAGGAACCGGTTTTATGACCGCTTTACTCAGTAAGTTATGTAAAAAAGTGATCAGTATTGACTATTA
TTCTGAATTTACTACCAATGCAAAACGCAAACTTGAAGAGCATAATTGTAATAATGTTGAATTAATTACCGGAGATGCTT
GTCGTGGATGGTTGGAAAGTGCACCATACGATGTTATCGTATTCACTGGTGCCATGGAAAAGCTGACAGATACCCATAAA
CTACAAATTTTACCTGGTGGCAAGTTATTCGCAATCCTTGGTAAATCCCCTGTAATGAAAGCTTATCTTTTTCAGTTGGA
TCATAATGCAATCTGGACAGAATCCATGCTTTTTGAAACGGATATACCTCCTCTGGTAGATCAATTGAAACCGAAAGAAT
TTGTTTTCTAG

Upstream 100 bases:

>100_bases
ATTTCACTTTCAAACATTAATGCTTAGACATAAGCTGAATTCTTGCTAAAATTGCTCCAAAGTTTTAACCTATCCAATGA
TTATTAAGACGGTAGAGCAA

Downstream 100 bases:

>100_bases
GGAAAGCTCGAATGAGAAAATCTCTGTTTTGTTGGATTTTAACTTTAGGTGTTTCAACCCATGCGTTTGCAACAGACCTA
ATGGATATCTATCAACAAGC

Product: protein-L-isoaspartate-O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MNHSARINMIKQQLRTGDVLNESILDLYDEILRHEFVPEPFSHFAYSDMQIPLAHGQRMLTPLEEGTILQSLDLKGHETV
LEVGTGTGFMTALLSKLCKKVISIDYYSEFTTNAKRKLEEHNCNNVELITGDACRGWLESAPYDVIVFTGAMEKLTDTHK
LQILPGGKLFAILGKSPVMKAYLFQLDHNAIWTESMLFETDIPPLVDQLKPKEFVF

Sequences:

>Translated_216_residues
MNHSARINMIKQQLRTGDVLNESILDLYDEILRHEFVPEPFSHFAYSDMQIPLAHGQRMLTPLEEGTILQSLDLKGHETV
LEVGTGTGFMTALLSKLCKKVISIDYYSEFTTNAKRKLEEHNCNNVELITGDACRGWLESAPYDVIVFTGAMEKLTDTHK
LQILPGGKLFAILGKSPVMKAYLFQLDHNAIWTESMLFETDIPPLVDQLKPKEFVF
>Mature_216_residues
MNHSARINMIKQQLRTGDVLNESILDLYDEILRHEFVPEPFSHFAYSDMQIPLAHGQRMLTPLEEGTILQSLDLKGHETV
LEVGTGTGFMTALLSKLCKKVISIDYYSEFTTNAKRKLEEHNCNNVELITGDACRGWLESAPYDVIVFTGAMEKLTDTHK
LQILPGGKLFAILGKSPVMKAYLFQLDHNAIWTESMLFETDIPPLVDQLKPKEFVF

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789100, Length=172, Percent_Identity=30.2325581395349, Blast_Score=101, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 24503; Mature: 24503

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHSARINMIKQQLRTGDVLNESILDLYDEILRHEFVPEPFSHFAYSDMQIPLAHGQRML
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEHHCCCHHC
TPLEEGTILQSLDLKGHETVLEVGTGTGFMTALLSKLCKKVISIDYYSEFTTNAKRKLEE
CCCCCCCEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
HNCNNVELITGDACRGWLESAPYDVIVFTGAMEKLTDTHKLQILPGGKLFAILGKSPVMK
CCCCCEEEEECHHHHHHHHCCCCEEEEEECCHHHHCCCCEEEEECCCCEEEEECCCHHHH
AYLFQLDHNAIWTESMLFETDIPPLVDQLKPKEFVF
HHHHHHCCCCEEHHHHHEECCCCHHHHHCCCHHCCC
>Mature Secondary Structure
MNHSARINMIKQQLRTGDVLNESILDLYDEILRHEFVPEPFSHFAYSDMQIPLAHGQRML
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEHHCCCHHC
TPLEEGTILQSLDLKGHETVLEVGTGTGFMTALLSKLCKKVISIDYYSEFTTNAKRKLEE
CCCCCCCEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
HNCNNVELITGDACRGWLESAPYDVIVFTGAMEKLTDTHKLQILPGGKLFAILGKSPVMK
CCCCCEEEEECHHHHHHHHCCCCEEEEEECCHHHHCCCCEEEEECCCCEEEEECCCHHHH
AYLFQLDHNAIWTESMLFETDIPPLVDQLKPKEFVF
HHHHHHCCCCEEHHHHHEECCCCHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA