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The map label for this gene is fadD21 [H]

Identifier: 148359771

GI number: 148359771

Start: 2636332

End: 2638077

Strand: Direct

Name: fadD21 [H]

Synonym: LPC_1696

Alternate gene names: 148359771

Gene position: 2636332-2638077 (Clockwise)

Preceding gene: 148359770

Following gene: 148359772

Centisome position: 73.71

GC content: 41.7

Gene sequence:

>1746_bases
GTGAAAAAAGAATATTTGCAGTGCCAGTCTCTGGTGGACGTCGTCAGGTTACGGGCTTTGCATAGCCCTAAAAAGAAAAG
CTGTACTTTTCTGAACAAAGAACTAGAAGAGACGATGACCTATGAGCAGCTGGATCTACACGCCAAAGCAATTGCGGCAA
CTTTGCAAGCAAAAGGAGCAAAACCTGGGGATAGGGTCTTGTTATTATTTGCACCAGGATTGCCCCTTATCCAGGCATTT
TTGGGCTGCCTTTATGCCGGCTGCATTGCTGTACCCATTTACCCACCGGCTCAAGAAAAATTATTGGATAAAGCACAACG
CATTGTTACCAACTCAAAACCGGTCATAGTACTAATGATTGCGGATCACATCAAAAAATTCACCGCAGACGAATTAAATA
CAAATCCCCAATTCCTGAAAATTCCTGCTATTGCGCTTGAGAGCATTGAGTTAAACAGAAGTAGTAGTTGGCAACCAACC
TCCATTAAGAGCAATGACATTGCGTTTCTACAATATACTTCCGGCTCAACCATGCACCCTAAAGGAGTGATGGTAAGCCA
CCATAATTTGCTGGATAATCTGGGTAAAATTTTTACCTCTTTTCATATGAATGATGAAACCATTATTTTCAGCTGGCTGC
CCCCACATCATGATATGGGTTTGATTGGCTGCATTCTTACACCCATCTATGGTGGAATTCAGGCAATCATGATGTCCCCT
TTCTCATTTTTACAAAACCCGCTTTCCTGGTTAAAACATATTACCAAATACAGAGCCACTATCAGTGGCAGCCCCAATTT
CGCTTACGATTATTGTGTCAAGCGGATCAGGGAAGAAAAAAAAGAAGGGCTGGATTTAAGTTCATGGGTGACCGCATTCA
ATGGCGCTGAGCCAGTGCGAGCAGAAACCATGGAACATTTTTATCAGGCATTTAAAGACTATGGATTTCGTAAAGAAGCC
TTCTACCCATGCTATGGCCTGGCCGAGGCTACTTTGTTAGTGACCGGAGGAACACCAGGAAATCCATACAAAACGTTAAC
TCTGGCCAAAGAACAATTTCAGGATCATCGCGTGCATTTTGCAGACGATAACAGTCCGGGCAGTTACAAGCTAGTCAGCA
GTGGTAATCCCATTCAAGCAGTTAAAATTATAGATCCTGATACCTTGATCCCATGTGATTTTGACCAGGTTGGTGAAATT
TGGGTACAAAGTAACAGTGTCGCAAAAGGATATTGGAATCAACCCGAAGAAACGAAGCATGCGTTCGCAGGAAAAATTAA
AGACGATGAGAGTAGCGCAATCTATTTAAGAACTGGGGACTTGGGCTTTCTCCATGAAAATGAGCTATACGTTACTGGAC
GCATTAAAGACTTAATTATTATTTATGGTAAAAATCATTACCCTCAGGACATAGAGTTCAGCCTGATGCATTCTCCTCTC
CATCACGTATTGGGCAAATGCGCTGCTTTTGTGATTCAGGAGGAGCATGAATATAAACTGACTGTGATGTGTGAAGTAAA
AAATCGATTCATGGATGACGTAGCTCAAGACAATTTATTCAATGAGATTTTTGAGCTTGTTTACGAAAACCACCAATTGG
AGGTACATACTATAGTCCTGATTCCTCTTAAAGCAATGCCACATACTACCAGCGGAAAAATTCGCAGGAATTTTTGCCGA
AAACATCTTTTGGATAAAACTCTGCCAATAGTAGCTACCTGGCAACTCAATAAAATTGAGGAATAA

Upstream 100 bases:

>100_bases
CAGGAAAAATTCTCCTGATGAGTGTTGGTGGTGGTCTATCCTATGCGGGACAAGTCCTAAACTATCAAAAAGCATCACAC
AGCAATTAAGGTGTTTTATA

Downstream 100 bases:

>100_bases
AATCATGCTGTTTTTGGCATCAGATGTTCCAAACAAAGTAGCAATTACCCAGGACAATCGAGCAATAACTTTTGATGAAT
TAACGCATGAAGTTTTAGCA

Product: saframycin Mx1 synthetase B

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 581; Mature: 581

Protein sequence:

>581_residues
MKKEYLQCQSLVDVVRLRALHSPKKKSCTFLNKELEETMTYEQLDLHAKAIAATLQAKGAKPGDRVLLLFAPGLPLIQAF
LGCLYAGCIAVPIYPPAQEKLLDKAQRIVTNSKPVIVLMIADHIKKFTADELNTNPQFLKIPAIALESIELNRSSSWQPT
SIKSNDIAFLQYTSGSTMHPKGVMVSHHNLLDNLGKIFTSFHMNDETIIFSWLPPHHDMGLIGCILTPIYGGIQAIMMSP
FSFLQNPLSWLKHITKYRATISGSPNFAYDYCVKRIREEKKEGLDLSSWVTAFNGAEPVRAETMEHFYQAFKDYGFRKEA
FYPCYGLAEATLLVTGGTPGNPYKTLTLAKEQFQDHRVHFADDNSPGSYKLVSSGNPIQAVKIIDPDTLIPCDFDQVGEI
WVQSNSVAKGYWNQPEETKHAFAGKIKDDESSAIYLRTGDLGFLHENELYVTGRIKDLIIIYGKNHYPQDIEFSLMHSPL
HHVLGKCAAFVIQEEHEYKLTVMCEVKNRFMDDVAQDNLFNEIFELVYENHQLEVHTIVLIPLKAMPHTTSGKIRRNFCR
KHLLDKTLPIVATWQLNKIEE

Sequences:

>Translated_581_residues
MKKEYLQCQSLVDVVRLRALHSPKKKSCTFLNKELEETMTYEQLDLHAKAIAATLQAKGAKPGDRVLLLFAPGLPLIQAF
LGCLYAGCIAVPIYPPAQEKLLDKAQRIVTNSKPVIVLMIADHIKKFTADELNTNPQFLKIPAIALESIELNRSSSWQPT
SIKSNDIAFLQYTSGSTMHPKGVMVSHHNLLDNLGKIFTSFHMNDETIIFSWLPPHHDMGLIGCILTPIYGGIQAIMMSP
FSFLQNPLSWLKHITKYRATISGSPNFAYDYCVKRIREEKKEGLDLSSWVTAFNGAEPVRAETMEHFYQAFKDYGFRKEA
FYPCYGLAEATLLVTGGTPGNPYKTLTLAKEQFQDHRVHFADDNSPGSYKLVSSGNPIQAVKIIDPDTLIPCDFDQVGEI
WVQSNSVAKGYWNQPEETKHAFAGKIKDDESSAIYLRTGDLGFLHENELYVTGRIKDLIIIYGKNHYPQDIEFSLMHSPL
HHVLGKCAAFVIQEEHEYKLTVMCEVKNRFMDDVAQDNLFNEIFELVYENHQLEVHTIVLIPLKAMPHTTSGKIRRNFCR
KHLLDKTLPIVATWQLNKIEE
>Mature_581_residues
MKKEYLQCQSLVDVVRLRALHSPKKKSCTFLNKELEETMTYEQLDLHAKAIAATLQAKGAKPGDRVLLLFAPGLPLIQAF
LGCLYAGCIAVPIYPPAQEKLLDKAQRIVTNSKPVIVLMIADHIKKFTADELNTNPQFLKIPAIALESIELNRSSSWQPT
SIKSNDIAFLQYTSGSTMHPKGVMVSHHNLLDNLGKIFTSFHMNDETIIFSWLPPHHDMGLIGCILTPIYGGIQAIMMSP
FSFLQNPLSWLKHITKYRATISGSPNFAYDYCVKRIREEKKEGLDLSSWVTAFNGAEPVRAETMEHFYQAFKDYGFRKEA
FYPCYGLAEATLLVTGGTPGNPYKTLTLAKEQFQDHRVHFADDNSPGSYKLVSSGNPIQAVKIIDPDTLIPCDFDQVGEI
WVQSNSVAKGYWNQPEETKHAFAGKIKDDESSAIYLRTGDLGFLHENELYVTGRIKDLIIIYGKNHYPQDIEFSLMHSPL
HHVLGKCAAFVIQEEHEYKLTVMCEVKNRFMDDVAQDNLFNEIFELVYENHQLEVHTIVLIPLKAMPHTTSGKIRRNFCR
KHLLDKTLPIVATWQLNKIEE

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI45827692, Length=602, Percent_Identity=27.7408637873754, Blast_Score=175, Evalue=1e-43,
Organism=Homo sapiens, GI225735629, Length=602, Percent_Identity=27.7408637873754, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI44888818, Length=596, Percent_Identity=26.510067114094, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI55749758, Length=516, Percent_Identity=28.4883720930233, Blast_Score=164, Evalue=3e-40,
Organism=Homo sapiens, GI225735625, Length=596, Percent_Identity=23.3221476510067, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI45827694, Length=546, Percent_Identity=23.4432234432234, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI225735627, Length=494, Percent_Identity=23.8866396761134, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI45827696, Length=494, Percent_Identity=23.8866396761134, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI45827698, Length=466, Percent_Identity=24.2489270386266, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI83745141, Length=437, Percent_Identity=25.629290617849, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1788107, Length=519, Percent_Identity=26.7822736030828, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI145693145, Length=557, Percent_Identity=22.0825852782765, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI193202679, Length=571, Percent_Identity=23.292469352014, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17531443, Length=573, Percent_Identity=23.9092495636998, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17558820, Length=447, Percent_Identity=23.2662192393736, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71994690, Length=372, Percent_Identity=25, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71994694, Length=372, Percent_Identity=25, Blast_Score=75, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI71994703, Length=372, Percent_Identity=25, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24654656, Length=466, Percent_Identity=27.8969957081545, Blast_Score=155, Evalue=6e-38,
Organism=Drosophila melanogaster, GI18859661, Length=552, Percent_Identity=23.0072463768116, Blast_Score=86, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 65834; Mature: 65834

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKEYLQCQSLVDVVRLRALHSPKKKSCTFLNKELEETMTYEQLDLHAKAIAATLQAKGA
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KPGDRVLLLFAPGLPLIQAFLGCLYAGCIAVPIYPPAQEKLLDKAQRIVTNSKPVIVLMI
CCCCEEEEEEECCHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEE
ADHIKKFTADELNTNPQFLKIPAIALESIELNRSSSWQPTSIKSNDIAFLQYTSGSTMHP
HHHHHHHHHHHCCCCCCEEEECCEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCC
KGVMVSHHNLLDNLGKIFTSFHMNDETIIFSWLPPHHDMGLIGCILTPIYGGIQAIMMSP
CCEEEEHHHHHHHHHHHHHHEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCH
FSFLQNPLSWLKHITKYRATISGSPNFAYDYCVKRIREEKKEGLDLSSWVTAFNGAEPVR
HHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHH
AETMEHFYQAFKDYGFRKEAFYPCYGLAEATLLVTGGTPGNPYKTLTLAKEQFQDHRVHF
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEEHHHHHCCCEEE
ADDNSPGSYKLVSSGNPIQAVKIIDPDTLIPCDFDQVGEIWVQSNSVAKGYWNQPEETKH
ECCCCCCCEEEEECCCCEEEEEEECCCCCCCCCHHHHCCEEEECCCEECCCCCCCHHHHH
AFAGKIKDDESSAIYLRTGDLGFLHENELYVTGRIKDLIIIYGKNHYPQDIEFSLMHSPL
HHCCCCCCCCCCEEEEEECCEEEEECCCEEEEEEEEEEEEEECCCCCCCCCCHHHHHHHH
HHVLGKCAAFVIQEEHEYKLTVMCEVKNRFMDDVAQDNLFNEIFELVYENHQLEVHTIVL
HHHHHHHHHHHEECCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEE
IPLKAMPHTTSGKIRRNFCRKHLLDKTLPIVATWQLNKIEE
EEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCC
>Mature Secondary Structure
MKKEYLQCQSLVDVVRLRALHSPKKKSCTFLNKELEETMTYEQLDLHAKAIAATLQAKGA
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KPGDRVLLLFAPGLPLIQAFLGCLYAGCIAVPIYPPAQEKLLDKAQRIVTNSKPVIVLMI
CCCCEEEEEEECCHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEE
ADHIKKFTADELNTNPQFLKIPAIALESIELNRSSSWQPTSIKSNDIAFLQYTSGSTMHP
HHHHHHHHHHHCCCCCCEEEECCEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCC
KGVMVSHHNLLDNLGKIFTSFHMNDETIIFSWLPPHHDMGLIGCILTPIYGGIQAIMMSP
CCEEEEHHHHHHHHHHHHHHEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCH
FSFLQNPLSWLKHITKYRATISGSPNFAYDYCVKRIREEKKEGLDLSSWVTAFNGAEPVR
HHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHH
AETMEHFYQAFKDYGFRKEAFYPCYGLAEATLLVTGGTPGNPYKTLTLAKEQFQDHRVHF
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEEHHHHHCCCEEE
ADDNSPGSYKLVSSGNPIQAVKIIDPDTLIPCDFDQVGEIWVQSNSVAKGYWNQPEETKH
ECCCCCCCEEEEECCCCEEEEEEECCCCCCCCCHHHHCCEEEECCCEECCCCCCCHHHHH
AFAGKIKDDESSAIYLRTGDLGFLHENELYVTGRIKDLIIIYGKNHYPQDIEFSLMHSPL
HHCCCCCCCCCCEEEEEECCEEEEECCCEEEEEEEEEEEEEECCCCCCCCCCHHHHHHHH
HHVLGKCAAFVIQEEHEYKLTVMCEVKNRFMDDVAQDNLFNEIFELVYENHQLEVHTIVL
HHHHHHHHHHHEECCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEE
IPLKAMPHTTSGKIRRNFCRKHLLDKTLPIVATWQLNKIEE
EEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]