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The map label for this gene is gltX [H]

Identifier: 148359458

GI number: 148359458

Start: 2222279

End: 2223691

Strand: Reverse

Name: gltX [H]

Synonym: LPC_1365

Alternate gene names: 148359458

Gene position: 2223691-2222279 (Counterclockwise)

Preceding gene: 148359461

Following gene: 148359457

Centisome position: 62.18

GC content: 41.12

Gene sequence:

>1413_bases
ATGACTGTTAGAACCCGATTTGCCCCAAGCCCTACTGGTTTTTTACACGTTGGTGGGGTAAGGACTGCATTATTTTCATG
GCTATATGCAAAACATCACAATGGGCAATTTATACTGCGTATAGAAGATACAGACAGAGAGCGTTCAACTCAAGAATCTG
TACAGGCTATTTTAGACGGAATGGCCTGGTTAGGGCTTAACTTCGATGAAGGTCCCTATTATCAGACTGAGCGATATGCT
CGTTATCAACAAGTAGCGCAGCAACTTTTGAAAGAAGGAAAAGCTTATCGCTGTCAATGCAGTAAGGAGAGACTGGAAGC
TTTAAGAGAGGCTCAATTAGCCGCCAAGGAAAAGCCACGCTATGATGGGCATTGCAGAAACCAAACTCTTCCTGATAGTG
GAGTTCCTTATGTTATTCGCTTTAGAAATCCTGATGCGGGTATAGTGTCTTTCCACGATGAGGTTTATGGTGACATTCAT
GTGGATAATAGCGAACTGGATGATTTGATTTTGGTTCGTTCTGATGGGCACCCCACCTATAATTTTGCTGTAGTTATCGA
TGATTGGGATATGAAAATAACCCATGTTATTCGCGGGGATGATCATATCAACAATACTCCGAGACAAATTAATTTATTCA
AGGCATTGGATGCGCCAGTACCTGTTTTTGCCCATTTGCCCATGATTTTGGGTGAAGATGGCAAACGGTTGTCAAAGCGA
CATGGTGCAGTAAGTGTCTTGCAGTTCAAGGAGCTGGGTGTATTGCCTCATGCTTTATTGAATTATTTAGTTCGTTTAGG
ATGGTCTCATGGCGATCAGGAAATTTTTAGTGTACAAGAAATGATAACCAGCTTTGACTTGAAAAATGTCAGTCGAGGCG
TCTCAAGTTTCAATTATGATAAGTTGTATTGGTTGAATCAGCATTATCAAAAAAGTGACTCTCCAGAATCAGTTGCCAAT
GCGTTGCAATGGCATTTTGAGCAGGCAGGAATTGATTTGAATCAAGGTCCCGATTTGAAGGATTTGGTCGCAGTTCAGGC
AGAGAGATGTAAGTCATTAGCAGAAATGTGTCAAATTAGTCAGTATTTTTATACAGATACCATTGAATATAATGAAGATG
CTGTTAAAAAACACTTGCGGCCAGTTGTTCTGGAGCCTTTAATGGTATTACATGAGCGCCTAAAAGCGCTTGACGAATGG
AAAAATGATAAAATTCAAGAATGCATTAATGATGTGAGCCTGCAATTTGATTTGAATTTAGGCAAAATCGCTCAACCGTT
GCGTGTCGCCGTGACCGGATCCGGAACATCTCCTTCCATTGACATGACATTGGCTTTGTTAGGTAAAAACAAATCAATCA
AACGTCTTGAAGACGCTTTGGAGAAAATACGAGCCAGAGCCAGTGCTGTTTAG

Upstream 100 bases:

>100_bases
ATAGCTCCTTTTAGTCTTAATTCAGCAGTTGAATCTTTGCGTTCATGGTAAACTCGGCTATTATAACTTGATTATTTTAT
TCCTTACAATGGAAGATATT

Downstream 100 bases:

>100_bases
AATCTAAATATTTATTGATTTTAAGCTGAACGCAAAAAGATCTGTATTTTGAGAAGTATTCATATTTCAAGTGGGCAATG
AAAACTACAATTTCGTTGGA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MTVRTRFAPSPTGFLHVGGVRTALFSWLYAKHHNGQFILRIEDTDRERSTQESVQAILDGMAWLGLNFDEGPYYQTERYA
RYQQVAQQLLKEGKAYRCQCSKERLEALREAQLAAKEKPRYDGHCRNQTLPDSGVPYVIRFRNPDAGIVSFHDEVYGDIH
VDNSELDDLILVRSDGHPTYNFAVVIDDWDMKITHVIRGDDHINNTPRQINLFKALDAPVPVFAHLPMILGEDGKRLSKR
HGAVSVLQFKELGVLPHALLNYLVRLGWSHGDQEIFSVQEMITSFDLKNVSRGVSSFNYDKLYWLNQHYQKSDSPESVAN
ALQWHFEQAGIDLNQGPDLKDLVAVQAERCKSLAEMCQISQYFYTDTIEYNEDAVKKHLRPVVLEPLMVLHERLKALDEW
KNDKIQECINDVSLQFDLNLGKIAQPLRVAVTGSGTSPSIDMTLALLGKNKSIKRLEDALEKIRARASAV

Sequences:

>Translated_470_residues
MTVRTRFAPSPTGFLHVGGVRTALFSWLYAKHHNGQFILRIEDTDRERSTQESVQAILDGMAWLGLNFDEGPYYQTERYA
RYQQVAQQLLKEGKAYRCQCSKERLEALREAQLAAKEKPRYDGHCRNQTLPDSGVPYVIRFRNPDAGIVSFHDEVYGDIH
VDNSELDDLILVRSDGHPTYNFAVVIDDWDMKITHVIRGDDHINNTPRQINLFKALDAPVPVFAHLPMILGEDGKRLSKR
HGAVSVLQFKELGVLPHALLNYLVRLGWSHGDQEIFSVQEMITSFDLKNVSRGVSSFNYDKLYWLNQHYQKSDSPESVAN
ALQWHFEQAGIDLNQGPDLKDLVAVQAERCKSLAEMCQISQYFYTDTIEYNEDAVKKHLRPVVLEPLMVLHERLKALDEW
KNDKIQECINDVSLQFDLNLGKIAQPLRVAVTGSGTSPSIDMTLALLGKNKSIKRLEDALEKIRARASAV
>Mature_469_residues
TVRTRFAPSPTGFLHVGGVRTALFSWLYAKHHNGQFILRIEDTDRERSTQESVQAILDGMAWLGLNFDEGPYYQTERYAR
YQQVAQQLLKEGKAYRCQCSKERLEALREAQLAAKEKPRYDGHCRNQTLPDSGVPYVIRFRNPDAGIVSFHDEVYGDIHV
DNSELDDLILVRSDGHPTYNFAVVIDDWDMKITHVIRGDDHINNTPRQINLFKALDAPVPVFAHLPMILGEDGKRLSKRH
GAVSVLQFKELGVLPHALLNYLVRLGWSHGDQEIFSVQEMITSFDLKNVSRGVSSFNYDKLYWLNQHYQKSDSPESVANA
LQWHFEQAGIDLNQGPDLKDLVAVQAERCKSLAEMCQISQYFYTDTIEYNEDAVKKHLRPVVLEPLMVLHERLKALDEWK
NDKIQECINDVSLQFDLNLGKIAQPLRVAVTGSGTSPSIDMTLALLGKNKSIKRLEDALEKIRARASAV

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=491, Percent_Identity=34.0122199592668, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI4826960, Length=108, Percent_Identity=37.037037037037, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI62241042, Length=212, Percent_Identity=24.5283018867925, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1788743, Length=467, Percent_Identity=59.3147751605996, Blast_Score=598, Evalue=1e-172,
Organism=Escherichia coli, GI226510925, Length=280, Percent_Identity=33.5714285714286, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1786895, Length=108, Percent_Identity=29.6296296296296, Blast_Score=66, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI71993394, Length=481, Percent_Identity=34.5114345114345, Blast_Score=233, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6324540, Length=500, Percent_Identity=33.6, Blast_Score=265, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6324742, Length=220, Percent_Identity=28.1818181818182, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6321192, Length=208, Percent_Identity=25.4807692307692, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24665321, Length=491, Percent_Identity=31.9755600814664, Blast_Score=259, Evalue=4e-69,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 53533; Mature: 53402

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVRTRFAPSPTGFLHVGGVRTALFSWLYAKHHNGQFILRIEDTDRERSTQESVQAILDG
CCCCCCCCCCCCCEEEECHHHHHHHHHHHHEECCCEEEEEEECCCCCHHHHHHHHHHHHH
MAWLGLNFDEGPYYQTERYARYQQVAQQLLKEGKAYRCQCSKERLEALREAQLAAKEKPR
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCC
YDGHCRNQTLPDSGVPYVIRFRNPDAGIVSFHDEVYGDIHVDNSELDDLILVRSDGHPTY
CCCCCCCCCCCCCCCCEEEEEECCCCCEEEECHHHCCEEEECCCCCCCEEEEECCCCCCE
NFAVVIDDWDMKITHVIRGDDHINNTPRQINLFKALDAPVPVFAHLPMILGEDGKRLSKR
EEEEEEECCCCEEEEEEECCCCCCCCCCCEEHHHHCCCCCCHHHHCHHHCCCCCHHHHHH
HGAVSVLQFKELGVLPHALLNYLVRLGWSHGDQEIFSVQEMITSFDLKNVSRGVSSFNYD
CCCEEEEHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KLYWLNQHYQKSDSPESVANALQWHFEQAGIDLNQGPDLKDLVAVQAERCKSLAEMCQIS
EEEEECHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
QYFYTDTIEYNEDAVKKHLRPVVLEPLMVLHERLKALDEWKNDKIQECINDVSLQFDLNL
HHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCEEEEECCH
GKIAQPLRVAVTGSGTSPSIDMTLALLGKNKSIKRLEDALEKIRARASAV
HHHCCCEEEEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TVRTRFAPSPTGFLHVGGVRTALFSWLYAKHHNGQFILRIEDTDRERSTQESVQAILDG
CCCCCCCCCCCCEEEECHHHHHHHHHHHHEECCCEEEEEEECCCCCHHHHHHHHHHHHH
MAWLGLNFDEGPYYQTERYARYQQVAQQLLKEGKAYRCQCSKERLEALREAQLAAKEKPR
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCC
YDGHCRNQTLPDSGVPYVIRFRNPDAGIVSFHDEVYGDIHVDNSELDDLILVRSDGHPTY
CCCCCCCCCCCCCCCCEEEEEECCCCCEEEECHHHCCEEEECCCCCCCEEEEECCCCCCE
NFAVVIDDWDMKITHVIRGDDHINNTPRQINLFKALDAPVPVFAHLPMILGEDGKRLSKR
EEEEEEECCCCEEEEEEECCCCCCCCCCCEEHHHHCCCCCCHHHHCHHHCCCCCHHHHHH
HGAVSVLQFKELGVLPHALLNYLVRLGWSHGDQEIFSVQEMITSFDLKNVSRGVSSFNYD
CCCEEEEHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KLYWLNQHYQKSDSPESVANALQWHFEQAGIDLNQGPDLKDLVAVQAERCKSLAEMCQIS
EEEEECHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
QYFYTDTIEYNEDAVKKHLRPVVLEPLMVLHERLKALDEWKNDKIQECINDVSLQFDLNL
HHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCEEEEECCH
GKIAQPLRVAVTGSGTSPSIDMTLALLGKNKSIKRLEDALEKIRARASAV
HHHCCCEEEEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA