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The map label for this gene is gshB [H]

Identifier: 148359393

GI number: 148359393

Start: 2149217

End: 2150176

Strand: Reverse

Name: gshB [H]

Synonym: LPC_1291

Alternate gene names: 148359393

Gene position: 2150176-2149217 (Counterclockwise)

Preceding gene: 148359394

Following gene: 148359389

Centisome position: 60.12

GC content: 39.38

Gene sequence:

>960_bases
ATGAAACTTGCGGTGCTTATGGATCCATTGGATCAAATAAAACCCTATAAAGACTCTACAGTAGCTATGATTAAAAGCGC
ACAAGGATTGGGATGGTCTTGCGCCTACTTTACCCTGAATGACTTATATTGTTGTGATGGGGGCGCTTATGCACGAGTCC
ATGAAATTCACATTGGAGATATAGAGAGTCTGAGTTGGGTTAAAACCATAGATTTGGGTAATAAGCCATTGAGAGATTTT
GATGTTATTTTGATGCGTAAGGATCCTCCATTTGATATGGAGTATATTTATGCAACTTACGCTTTGGATTTAGCGGAACA
AGAAGGGGTGTTGGTTGCAAATAAACCGCAAAGTTTACGTGACGCGAATGAGAAATTTTTTACCCTAAATTTTCCTCATT
GCTGTCCCCCTACTTTTGTATCCAGAGATGCTCGGCAATTAAAAGAGTTCTGGCAAATTCATCAACAAGTTATTTTTAAA
CCTCTTGAAGGAATGGGAGGTAATTCCGTTTTTTATGTTGACAAAGAAGGTAAAAATTTATCGGTCATTTTGGAGGTATT
AACCAAAGGGCAGACAAAATGTATTATGGCTCAGCGTTATATCCCGGATATTACAGTAAGCGGAGACAAACGTATTTTAT
TGATCAATGGCGAACCCGTTCCTTTTGCTTTGGCTCGCATTCCTGCAAAAGGAGATTTGCGGGGAAATTTGGCCGCAGGA
GCCAGGGGAGAAGTGGTAGCTATTACTGAAAGAGACAAATGGATATGTGAACAAATAGCTCCAGCTTTGAGAGAAAAAGG
TCTGTATTTTGTGGGTATCGACGTGATAGGTGATTATCTAACAGAGATTAATGTGACTAGTCCCACTTGTATTCGGGAAA
TTAGCACTGAAACTGGTTTGGATATCGCAGGTGAATATATGCGCTGCCTTGAAAAGTTACATACTTCTTTATCAAAATAA

Upstream 100 bases:

>100_bases
AGGTAGTTGACTGCCCAAATCGTTTTTATGTTTATGGGGTTATAGCTCGTTTGGCTGCACTGGCTGCAGCTCGAGAAATT
GCTGCAATTGGAGGCGAATG

Downstream 100 bases:

>100_bases
AGAACAGTCATTATTTTGATTTTGAAGTTCAGAGCTTAAAGAGGGTTGGTTTTGCATTTGGCTCAATACCAACCCTTATC
ATGACCTGGCAAGTCTCAAT

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKLAVLMDPLDQIKPYKDSTVAMIKSAQGLGWSCAYFTLNDLYCCDGGAYARVHEIHIGDIESLSWVKTIDLGNKPLRDF
DVILMRKDPPFDMEYIYATYALDLAEQEGVLVANKPQSLRDANEKFFTLNFPHCCPPTFVSRDARQLKEFWQIHQQVIFK
PLEGMGGNSVFYVDKEGKNLSVILEVLTKGQTKCIMAQRYIPDITVSGDKRILLINGEPVPFALARIPAKGDLRGNLAAG
ARGEVVAITERDKWICEQIAPALREKGLYFVGIDVIGDYLTEINVTSPTCIREISTETGLDIAGEYMRCLEKLHTSLSK

Sequences:

>Translated_319_residues
MKLAVLMDPLDQIKPYKDSTVAMIKSAQGLGWSCAYFTLNDLYCCDGGAYARVHEIHIGDIESLSWVKTIDLGNKPLRDF
DVILMRKDPPFDMEYIYATYALDLAEQEGVLVANKPQSLRDANEKFFTLNFPHCCPPTFVSRDARQLKEFWQIHQQVIFK
PLEGMGGNSVFYVDKEGKNLSVILEVLTKGQTKCIMAQRYIPDITVSGDKRILLINGEPVPFALARIPAKGDLRGNLAAG
ARGEVVAITERDKWICEQIAPALREKGLYFVGIDVIGDYLTEINVTSPTCIREISTETGLDIAGEYMRCLEKLHTSLSK
>Mature_319_residues
MKLAVLMDPLDQIKPYKDSTVAMIKSAQGLGWSCAYFTLNDLYCCDGGAYARVHEIHIGDIESLSWVKTIDLGNKPLRDF
DVILMRKDPPFDMEYIYATYALDLAEQEGVLVANKPQSLRDANEKFFTLNFPHCCPPTFVSRDARQLKEFWQIHQQVIFK
PLEGMGGNSVFYVDKEGKNLSVILEVLTKGQTKCIMAQRYIPDITVSGDKRILLINGEPVPFALARIPAKGDLRGNLAAG
ARGEVVAITERDKWICEQIAPALREKGLYFVGIDVIGDYLTEINVTSPTCIREISTETGLDIAGEYMRCLEKLHTSLSK

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=313, Percent_Identity=53.0351437699681, Blast_Score=319, Evalue=2e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 35734; Mature: 35734

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLAVLMDPLDQIKPYKDSTVAMIKSAQGLGWSCAYFTLNDLYCCDGGAYARVHEIHIGD
CEEEEEECCHHHCCCCCCCHHEEEHHCCCCCEEEEEEEECCEEEECCCCEEEEEEEEECC
IESLSWVKTIDLGNKPLRDFDVILMRKDPPFDMEYIYATYALDLAEQEGVLVANKPQSLR
CCCCCEEEEECCCCCCCCCEEEEEEECCCCCCHHEEEEEEEEEHHCCCCEEEECCCHHHH
DANEKFFTLNFPHCCPPTFVSRDARQLKEFWQIHQQVIFKPLEGMGGNSVFYVDKEGKNL
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCH
SVILEVLTKGQTKCIMAQRYIPDITVSGDKRILLINGEPVPFALARIPAKGDLRGNLAAG
HHHHHHHHCCCCEEEEHHHCCCCEEECCCCEEEEECCCCCCEEEEECCCCCCCCCCCCCC
ARGEVVAITERDKWICEQIAPALREKGLYFVGIDVIGDYLTEINVTSPTCIREISTETGL
CCCCEEEEECCCHHHHHHHHHHHHHCCEEEEEHHHHHHHHHEECCCCCHHHHHHCCCCCC
DIAGEYMRCLEKLHTSLSK
CHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLAVLMDPLDQIKPYKDSTVAMIKSAQGLGWSCAYFTLNDLYCCDGGAYARVHEIHIGD
CEEEEEECCHHHCCCCCCCHHEEEHHCCCCCEEEEEEEECCEEEECCCCEEEEEEEEECC
IESLSWVKTIDLGNKPLRDFDVILMRKDPPFDMEYIYATYALDLAEQEGVLVANKPQSLR
CCCCCEEEEECCCCCCCCCEEEEEEECCCCCCHHEEEEEEEEEHHCCCCEEEECCCHHHH
DANEKFFTLNFPHCCPPTFVSRDARQLKEFWQIHQQVIFKPLEGMGGNSVFYVDKEGKNL
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCH
SVILEVLTKGQTKCIMAQRYIPDITVSGDKRILLINGEPVPFALARIPAKGDLRGNLAAG
HHHHHHHHCCCCEEEEHHHCCCCEEECCCCEEEEECCCCCCEEEEECCCCCCCCCCCCCC
ARGEVVAITERDKWICEQIAPALREKGLYFVGIDVIGDYLTEINVTSPTCIREISTETGL
CCCCEEEEECCCHHHHHHHHHHHHHCCEEEEEHHHHHHHHHEECCCCCHHHHHHCCCCCC
DIAGEYMRCLEKLHTSLSK
CHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]