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The map label for this gene is uvrD [H]

Identifier: 148359389

GI number: 148359389

Start: 2144333

End: 2146498

Strand: Reverse

Name: uvrD [H]

Synonym: LPC_1287

Alternate gene names: 148359389

Gene position: 2146498-2144333 (Counterclockwise)

Preceding gene: 148359393

Following gene: 148359388

Centisome position: 60.02

GC content: 42.2

Gene sequence:

>2166_bases
ATGACCATAGCAGCACTTCTCAAGGGATTAAACGAAAAGCAACGTGACGCGGTAACCTCTCCATTAGGTAATATGCTGGT
TTTAGCAGGTGCAGGCAGTGGTAAAACAAAAGTCTTGGTGAGCCGAATTGCCTGGTTAATTGAAGAACAGCATCTCTCTC
CCCACGCTATATTAGCAGTAACATTTACGAATAAAGCGGCCGGTGAAATGAGATCGAGACTTAGCAGTATGTTGTCTACA
CCAACGTTGGGTTTATGGGTTGGTACTTTTCATGGATTATGCCATCGTTTATTGCGTCGCCACTACAAGGAGGCTAACCT
TCCAGAACAGTTTCATATTCTGGATTCGGAAGACCAGGCACGAGTTATCAAGCGAGTCATTCTTTCACTTAATCTTGATC
CTGAGCAATGGCAAGTCAAACAAGCGCAAGCATTTATTAATAGTAAAAAGGATGAAGGCTTGCGCCCACAACATATTAAT
GCACTGCATTATGGCCCGACCAAGACTTTGGTTTCAATTTATAAAGCGTATGAAGACGTTTGCCAAACCTCAGGGGTAAT
CGATTTCGCTGAACTATTATTACGAACTCATGAATTGCTACGAGATAATGAAGAGATTCTTGCTCATTACCGTGAACGCT
TCCAAGCTATTTTAGTTGATGAATTTCAGGATACCAATACGATTCAATATGCATGGATCCGTTTGCTTGCTGGTGATCAT
ACAGCGGTATTGGCTGTAGGAGATGATGATCAGTCAATTTATGGTTGGCGTGGGGCTAAAGTGGAAAATATTCAACAATT
TGTTCATGATTTTAAGGATACCCAAATCATAAGACTTGAACAAAATTACAGGTCAACTGCAATGATATTAAATGCAGCAA
ATGCATTGATTCACAACAACAGTACTCGTATGGGTAAAGAGTTATGGACTGCTGGTAGTGAAGGAGAAAAAATTCTTGTA
TACAGTGCATTCAATGAATTGGATGAGGCACGTTTTGTTACTGAACGAATTGGCATGGAATTGAATCAGGGCGCCAGTGC
GGATGAGATTGCAGTGTTGTATCGTTCGAATGCCCAATCCCGTGTTTTGGAAGAAGCTTTGTTACGTGTAGGTATAGCCT
ACAGGATATATGGAGGAGTCCGTTTCTTTGACAGGGCAGAAATTAAAGATACTTTGGCTTACTTACGATTGTTAGTTAAT
CCAAATGACGATACTGCGTTTGAAAGAGTGGTTAATTTTCCTACTCGAGGAATAGGAGAAAAAACATTGGATGAAGTTCG
CCAGTACGCCAGAGCAGAACAGTGTTCGTTGTGGGACGCTTCCAAGGGCATACTTCAATCCACAGGATTAGGGCAACGGG
GGAGCTTGGCATTAGCTAAGTTTATTGATTTGATCGAAAAATTACAGGTTGTGGTTGCTAATAAGGAGTTGGATGAGCAG
ATCTCTGATGTAATACAGCATAGTGGTTTATACGCCCACTTTAGCAAAATCAAAGGTGATAAATCAGAATCACGTGTGGA
TAACCTGCAAGAATTAATTAATGCTGCCAAACAATTTCGATATGAGTACGATGAAGAGGAGGAGTTACCGTTGGTCAATT
CATTCCTTGCTCATGCTTCATTAGAATCGGGGGAATTGCAGGCTGATGAACATGAGCGCTCCGTTCACTTGATGACCTTA
CATGCAGCCAAAGGGCTTGAGTTTCCGATTGTGTTTTTGGTTGGTATGGAAGAAGGAATATTTCCAGGCAGACAATCGAT
TGAAGAGCCTGGTCGCTTGGAGGAAGAGCGTCGATTATGTTATGTCGGTATGACTCGTGCCATGCGTAAATTGGTTTTAT
CCTATGCTGAGGTCAGAAGACAATATGGCCGTGAAGAGTATCACAGATCATCAAGATTTTTGCGTGAAATTCCTCAACAA
TTTCTTGATGAGGTACGAGTTAAATCACGTTCTCAGTGGCCCGGAACTACAAAAAGCAAATTGCCAGTGGCTGACGAGGT
GTCGGGCATTACAATTGGGCAAAATGTGCAGCATGCCAAATTTGGACAGGGTGTCGTTCTTGCCATTGAGGGCAGCGGGG
CCCATACAAGAGTTCAAGTAAAATTCAGTGAACATGGTGTAAAATGGTTGGTTTTGGCCTATGCTAATTTGACTGAATGC
CTGTAG

Upstream 100 bases:

>100_bases
ATCCAGGAGAATTTTTAGATTTTTTACCGTATCGATTATATCTTTGCTTTAGCAATTAAATTGAAGATAATACTTGCACT
TTTTCTGTTGGAAATATTCT

Downstream 100 bases:

>100_bases
TTATTGCATTTAATTTTTATAAAATATCTGAGGTACCCTGACTTGCAGTTTGTTGTTGTAATATTGAATTTTTTTTTCAT
GAATGCTAGGGTGCGTTAGG

Product: DNA-dependent helicase II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 721; Mature: 720

Protein sequence:

>721_residues
MTIAALLKGLNEKQRDAVTSPLGNMLVLAGAGSGKTKVLVSRIAWLIEEQHLSPHAILAVTFTNKAAGEMRSRLSSMLST
PTLGLWVGTFHGLCHRLLRRHYKEANLPEQFHILDSEDQARVIKRVILSLNLDPEQWQVKQAQAFINSKKDEGLRPQHIN
ALHYGPTKTLVSIYKAYEDVCQTSGVIDFAELLLRTHELLRDNEEILAHYRERFQAILVDEFQDTNTIQYAWIRLLAGDH
TAVLAVGDDDQSIYGWRGAKVENIQQFVHDFKDTQIIRLEQNYRSTAMILNAANALIHNNSTRMGKELWTAGSEGEKILV
YSAFNELDEARFVTERIGMELNQGASADEIAVLYRSNAQSRVLEEALLRVGIAYRIYGGVRFFDRAEIKDTLAYLRLLVN
PNDDTAFERVVNFPTRGIGEKTLDEVRQYARAEQCSLWDASKGILQSTGLGQRGSLALAKFIDLIEKLQVVVANKELDEQ
ISDVIQHSGLYAHFSKIKGDKSESRVDNLQELINAAKQFRYEYDEEEELPLVNSFLAHASLESGELQADEHERSVHLMTL
HAAKGLEFPIVFLVGMEEGIFPGRQSIEEPGRLEEERRLCYVGMTRAMRKLVLSYAEVRRQYGREEYHRSSRFLREIPQQ
FLDEVRVKSRSQWPGTTKSKLPVADEVSGITIGQNVQHAKFGQGVVLAIEGSGAHTRVQVKFSEHGVKWLVLAYANLTEC
L

Sequences:

>Translated_721_residues
MTIAALLKGLNEKQRDAVTSPLGNMLVLAGAGSGKTKVLVSRIAWLIEEQHLSPHAILAVTFTNKAAGEMRSRLSSMLST
PTLGLWVGTFHGLCHRLLRRHYKEANLPEQFHILDSEDQARVIKRVILSLNLDPEQWQVKQAQAFINSKKDEGLRPQHIN
ALHYGPTKTLVSIYKAYEDVCQTSGVIDFAELLLRTHELLRDNEEILAHYRERFQAILVDEFQDTNTIQYAWIRLLAGDH
TAVLAVGDDDQSIYGWRGAKVENIQQFVHDFKDTQIIRLEQNYRSTAMILNAANALIHNNSTRMGKELWTAGSEGEKILV
YSAFNELDEARFVTERIGMELNQGASADEIAVLYRSNAQSRVLEEALLRVGIAYRIYGGVRFFDRAEIKDTLAYLRLLVN
PNDDTAFERVVNFPTRGIGEKTLDEVRQYARAEQCSLWDASKGILQSTGLGQRGSLALAKFIDLIEKLQVVVANKELDEQ
ISDVIQHSGLYAHFSKIKGDKSESRVDNLQELINAAKQFRYEYDEEEELPLVNSFLAHASLESGELQADEHERSVHLMTL
HAAKGLEFPIVFLVGMEEGIFPGRQSIEEPGRLEEERRLCYVGMTRAMRKLVLSYAEVRRQYGREEYHRSSRFLREIPQQ
FLDEVRVKSRSQWPGTTKSKLPVADEVSGITIGQNVQHAKFGQGVVLAIEGSGAHTRVQVKFSEHGVKWLVLAYANLTEC
L
>Mature_720_residues
TIAALLKGLNEKQRDAVTSPLGNMLVLAGAGSGKTKVLVSRIAWLIEEQHLSPHAILAVTFTNKAAGEMRSRLSSMLSTP
TLGLWVGTFHGLCHRLLRRHYKEANLPEQFHILDSEDQARVIKRVILSLNLDPEQWQVKQAQAFINSKKDEGLRPQHINA
LHYGPTKTLVSIYKAYEDVCQTSGVIDFAELLLRTHELLRDNEEILAHYRERFQAILVDEFQDTNTIQYAWIRLLAGDHT
AVLAVGDDDQSIYGWRGAKVENIQQFVHDFKDTQIIRLEQNYRSTAMILNAANALIHNNSTRMGKELWTAGSEGEKILVY
SAFNELDEARFVTERIGMELNQGASADEIAVLYRSNAQSRVLEEALLRVGIAYRIYGGVRFFDRAEIKDTLAYLRLLVNP
NDDTAFERVVNFPTRGIGEKTLDEVRQYARAEQCSLWDASKGILQSTGLGQRGSLALAKFIDLIEKLQVVVANKELDEQI
SDVIQHSGLYAHFSKIKGDKSESRVDNLQELINAAKQFRYEYDEEEELPLVNSFLAHASLESGELQADEHERSVHLMTLH
AAKGLEFPIVFLVGMEEGIFPGRQSIEEPGRLEEERRLCYVGMTRAMRKLVLSYAEVRRQYGREEYHRSSRFLREIPQQF
LDEVRVKSRSQWPGTTKSKLPVADEVSGITIGQNVQHAKFGQGVVLAIEGSGAHTRVQVKFSEHGVKWLVLAYANLTECL

Specific function: Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the post-incision events of nucleotide exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=718, Percent_Identity=57.9387186629526, Blast_Score=864, Evalue=0.0,
Organism=Escherichia coli, GI48994965, Length=643, Percent_Identity=39.50233281493, Blast_Score=389, Evalue=1e-109,
Organism=Escherichia coli, GI1787196, Length=366, Percent_Identity=26.5027322404372, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6322369, Length=748, Percent_Identity=28.0748663101604, Blast_Score=197, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6324477, Length=644, Percent_Identity=22.8260869565217, Blast_Score=105, Evalue=2e-23,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005753
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 81555; Mature: 81424

Theoretical pI: Translated: 6.48; Mature: 6.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIAALLKGLNEKQRDAVTSPLGNMLVLAGAGSGKTKVLVSRIAWLIEEQHLSPHAILAV
CCHHHHHHCCCHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEE
TFTNKAAGEMRSRLSSMLSTPTLGLWVGTFHGLCHRLLRRHYKEANLPEQFHILDSEDQA
EECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHH
RVIKRVILSLNLDPEQWQVKQAQAFINSKKDEGLRPQHINALHYGPTKTLVSIYKAYEDV
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHH
CQTSGVIDFAELLLRTHELLRDNEEILAHYRERFQAILVDEFQDTNTIQYAWIRLLAGDH
HHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHEECCC
TAVLAVGDDDQSIYGWRGAKVENIQQFVHDFKDTQIIRLEQNYRSTAMILNAANALIHNN
EEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCC
STRMGKELWTAGSEGEKILVYSAFNELDEARFVTERIGMELNQGASADEIAVLYRSNAQS
CHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHH
RVLEEALLRVGIAYRIYGGVRFFDRAEIKDTLAYLRLLVNPNDDTAFERVVNFPTRGIGE
HHHHHHHHHHHHHHEEECCEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCH
KTLDEVRQYARAEQCSLWDASKGILQSTGLGQRGSLALAKFIDLIEKLQVVVANKELDEQ
HHHHHHHHHHHHHHCCCCHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHH
ISDVIQHSGLYAHFSKIKGDKSESRVDNLQELINAAKQFRYEYDEEEELPLVNSFLAHAS
HHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCC
LESGELQADEHERSVHLMTLHAAKGLEFPIVFLVGMEEGIFPGRQSIEEPGRLEEERRLC
CCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHH
YVGMTRAMRKLVLSYAEVRRQYGREEYHRSSRFLREIPQQFLDEVRVKSRSQWPGTTKSK
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
LPVADEVSGITIGQNVQHAKFGQGVVLAIEGSGAHTRVQVKFSEHGVKWLVLAYANLTEC
CCCCCCCCCEEECCCCCCCCCCCCEEEEEECCCCEEEEEEEECCCCCEEEEEEHHHHHHC
L
C
>Mature Secondary Structure 
TIAALLKGLNEKQRDAVTSPLGNMLVLAGAGSGKTKVLVSRIAWLIEEQHLSPHAILAV
CHHHHHHCCCHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEE
TFTNKAAGEMRSRLSSMLSTPTLGLWVGTFHGLCHRLLRRHYKEANLPEQFHILDSEDQA
EECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHH
RVIKRVILSLNLDPEQWQVKQAQAFINSKKDEGLRPQHINALHYGPTKTLVSIYKAYEDV
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHH
CQTSGVIDFAELLLRTHELLRDNEEILAHYRERFQAILVDEFQDTNTIQYAWIRLLAGDH
HHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHEECCC
TAVLAVGDDDQSIYGWRGAKVENIQQFVHDFKDTQIIRLEQNYRSTAMILNAANALIHNN
EEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCC
STRMGKELWTAGSEGEKILVYSAFNELDEARFVTERIGMELNQGASADEIAVLYRSNAQS
CHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHH
RVLEEALLRVGIAYRIYGGVRFFDRAEIKDTLAYLRLLVNPNDDTAFERVVNFPTRGIGE
HHHHHHHHHHHHHHEEECCEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCH
KTLDEVRQYARAEQCSLWDASKGILQSTGLGQRGSLALAKFIDLIEKLQVVVANKELDEQ
HHHHHHHHHHHHHHCCCCHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHH
ISDVIQHSGLYAHFSKIKGDKSESRVDNLQELINAAKQFRYEYDEEEELPLVNSFLAHAS
HHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCC
LESGELQADEHERSVHLMTLHAAKGLEFPIVFLVGMEEGIFPGRQSIEEPGRLEEERRLC
CCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHH
YVGMTRAMRKLVLSYAEVRRQYGREEYHRSSRFLREIPQQFLDEVRVKSRSQWPGTTKSK
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
LPVADEVSGITIGQNVQHAKFGQGVVLAIEGSGAHTRVQVKFSEHGVKWLVLAYANLTEC
CCCCCCCCCEEECCCCCCCCCCCCEEEEEECCCCEEEEEEEECCCCCEEEEEEHHHHHHC
L
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6379604; 2943729; 1379743; 9278503; 6324092; 2254268; 8419285 [H]