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The map label for this gene is pmbA [H]

Identifier: 148359315

GI number: 148359315

Start: 2057245

End: 2058603

Strand: Reverse

Name: pmbA [H]

Synonym: LPC_1212

Alternate gene names: 148359315

Gene position: 2058603-2057245 (Counterclockwise)

Preceding gene: 148359321

Following gene: 148359310

Centisome position: 57.56

GC content: 40.47

Gene sequence:

>1359_bases
ATGCAAATTAAACCGCAAAATAACAATAGTGAAGTATATAAGTCAACAACAGATTTAAATCGAGTAATAAAAGATGTTTT
AGAACTTGCCAAAAAAGAAGGTGCCACTGATGCAGCAGTAGCAGTAAATTATGATCGAGGTTTTTCAGTCGATGTGAGAA
TGGGGGAAGTGGAGACAGTCGCTTTTAGCGAAGATAAAGGAGTTGGTTTAACCGTATACATAGGTCAGCGCAAAGGGGGA
GCAAGCAGTACAGATACCTCTCCTGAAGCATTACAATCTTTGGTTAAAGCAGCCTGTGAGATAGCGAAGGTTAGTGCCGA
AGATCCTTGCTTTGGATTAGCTGATAAGGAATTGATGACAAAGAATCATCCAGATCTTGATTTGTATCATTCCTGGGACA
TTACCCCACAACACGCTATAGATTTGGCTTTAAAATGCGAATCACATGCCTTGTCTTTAGATAAACGGATTACTAATTCA
GATGGTGTTAATGTTTCTTCCTATGAATCTCATCACGGTTATGCCAATACCCATGGTGGAGAGGGGTTTATTCATAGTAC
TCGACATAGCGTAAGTTGCTCGGTAATCGCCAAAGAAGGTGATGAAATGCAAAGAGACTACGATTATACGACTGCCAGAA
ATGCAGAAAATTTAGTCTCAGCTGATTTGATAGCAGCTAAAGCTGTTGAAAGGGCATTAAGTCGTCTGGGATCAAAACAA
ATTAAAACACAAATGACTCCGGTTATTTTTTCTTCAAGAGTTTCCAGTGGATTATTGGGTAGCTTTGTGAGCGCTATTAG
TGGTTCAAATTTATATAGAAAAAATTCCTTTTTGCTTGATTCCATTGGACAACAATTGTTTCCGGAGTTTGTTCATATTT
ACGAACAGCCACATTTAAAAGGTGCTTTGGGGAGCGCCCCTTTTGATGGGGAAGGTGTGCCTACGCGACCCAATGTATTA
GTTGAGAAGGGGCGCTTGCTTCAATATGTTCTTGGCAGTTATTCGGCGCGAAAAATGGGTTTAAGGACAACAGCAAATAG
TGATGGCGTTCATAATTTAACCATTGATCCTACTTGCGGGGATCTTGGTGACCTTTTGAAAATGATGCGGAAAGGGTTGC
TGGTTACAGAACTAATGGGTCAGGGTGTGAATGGTATTACCGGAGATTATTCACGTGGGGCAAGTGGATACTGGGTTGAG
GATGGGGTCATACAATATCCGGTTGATCAGATTACTATCGCAGGAAATCTTAAAGAAATGTACCGGATGATATTGGCAGT
AGGCAGTGACATTAATCCGAATATTTCCATGCGCTGTGGCTCTATTTTAATAGAAAATATGATGGTTGCAGGGAAATAA

Upstream 100 bases:

>100_bases
GCAAGAGCCATAAATTCAATTTGTTTGCAGGATAAACATTGGTTTGCGAAGACATGTTATTGTATCCTTATGTACATCAT
TCAACACCACGGAATCTCAT

Downstream 100 bases:

>100_bases
AATGCCCCAAACCATCGAGGTTAACCACTTGCTCTGTAATCTTGATGATAGTTCTCTGGAACATTTTTAGCGATATACCT
GGCTGCTTGCAGCCAGCTTT

Product: peptide maturation protein PmbA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MQIKPQNNNSEVYKSTTDLNRVIKDVLELAKKEGATDAAVAVNYDRGFSVDVRMGEVETVAFSEDKGVGLTVYIGQRKGG
ASSTDTSPEALQSLVKAACEIAKVSAEDPCFGLADKELMTKNHPDLDLYHSWDITPQHAIDLALKCESHALSLDKRITNS
DGVNVSSYESHHGYANTHGGEGFIHSTRHSVSCSVIAKEGDEMQRDYDYTTARNAENLVSADLIAAKAVERALSRLGSKQ
IKTQMTPVIFSSRVSSGLLGSFVSAISGSNLYRKNSFLLDSIGQQLFPEFVHIYEQPHLKGALGSAPFDGEGVPTRPNVL
VEKGRLLQYVLGSYSARKMGLRTTANSDGVHNLTIDPTCGDLGDLLKMMRKGLLVTELMGQGVNGITGDYSRGASGYWVE
DGVIQYPVDQITIAGNLKEMYRMILAVGSDINPNISMRCGSILIENMMVAGK

Sequences:

>Translated_452_residues
MQIKPQNNNSEVYKSTTDLNRVIKDVLELAKKEGATDAAVAVNYDRGFSVDVRMGEVETVAFSEDKGVGLTVYIGQRKGG
ASSTDTSPEALQSLVKAACEIAKVSAEDPCFGLADKELMTKNHPDLDLYHSWDITPQHAIDLALKCESHALSLDKRITNS
DGVNVSSYESHHGYANTHGGEGFIHSTRHSVSCSVIAKEGDEMQRDYDYTTARNAENLVSADLIAAKAVERALSRLGSKQ
IKTQMTPVIFSSRVSSGLLGSFVSAISGSNLYRKNSFLLDSIGQQLFPEFVHIYEQPHLKGALGSAPFDGEGVPTRPNVL
VEKGRLLQYVLGSYSARKMGLRTTANSDGVHNLTIDPTCGDLGDLLKMMRKGLLVTELMGQGVNGITGDYSRGASGYWVE
DGVIQYPVDQITIAGNLKEMYRMILAVGSDINPNISMRCGSILIENMMVAGK
>Mature_452_residues
MQIKPQNNNSEVYKSTTDLNRVIKDVLELAKKEGATDAAVAVNYDRGFSVDVRMGEVETVAFSEDKGVGLTVYIGQRKGG
ASSTDTSPEALQSLVKAACEIAKVSAEDPCFGLADKELMTKNHPDLDLYHSWDITPQHAIDLALKCESHALSLDKRITNS
DGVNVSSYESHHGYANTHGGEGFIHSTRHSVSCSVIAKEGDEMQRDYDYTTARNAENLVSADLIAAKAVERALSRLGSKQ
IKTQMTPVIFSSRVSSGLLGSFVSAISGSNLYRKNSFLLDSIGQQLFPEFVHIYEQPHLKGALGSAPFDGEGVPTRPNVL
VEKGRLLQYVLGSYSARKMGLRTTANSDGVHNLTIDPTCGDLGDLLKMMRKGLLVTELMGQGVNGITGDYSRGASGYWVE
DGVIQYPVDQITIAGNLKEMYRMILAVGSDINPNISMRCGSILIENMMVAGK

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=447, Percent_Identity=48.0984340044743, Blast_Score=421, Evalue=1e-119,
Organism=Escherichia coli, GI1789640, Length=397, Percent_Identity=23.9294710327456, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 48981; Mature: 48981

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIKPQNNNSEVYKSTTDLNRVIKDVLELAKKEGATDAAVAVNYDRGFSVDVRMGEVETV
CCCCCCCCCCHHEECHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCEEEE
AFSEDKGVGLTVYIGQRKGGASSTDTSPEALQSLVKAACEIAKVSAEDPCFGLADKELMT
EECCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
KNHPDLDLYHSWDITPQHAIDLALKCESHALSLDKRITNSDGVNVSSYESHHGYANTHGG
CCCCCCEEEEECCCCHHHHHEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
EGFIHSTRHSVSCSVIAKEGDEMQRDYDYTTARNAENLVSADLIAAKAVERALSRLGSKQ
CCCEECCCCCEEEEEEECCCHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCHHH
IKTQMTPVIFSSRVSSGLLGSFVSAISGSNLYRKNSFLLDSIGQQLFPEFVHIYEQPHLK
HHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHHHCCCCCC
GALGSAPFDGEGVPTRPNVLVEKGRLLQYVLGSYSARKMGLRTTANSDGVHNLTIDPTCG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHCCCEECCCCCCCEEEEECCCCC
DLGDLLKMMRKGLLVTELMGQGVNGITGDYSRGASGYWVEDGVIQYPVDQITIAGNLKEM
CHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCEEECCCEEECCCCEEEEECCHHHH
YRMILAVGSDINPNISMRCGSILIENMMVAGK
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQIKPQNNNSEVYKSTTDLNRVIKDVLELAKKEGATDAAVAVNYDRGFSVDVRMGEVETV
CCCCCCCCCCHHEECHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCEEEE
AFSEDKGVGLTVYIGQRKGGASSTDTSPEALQSLVKAACEIAKVSAEDPCFGLADKELMT
EECCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
KNHPDLDLYHSWDITPQHAIDLALKCESHALSLDKRITNSDGVNVSSYESHHGYANTHGG
CCCCCCEEEEECCCCHHHHHEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
EGFIHSTRHSVSCSVIAKEGDEMQRDYDYTTARNAENLVSADLIAAKAVERALSRLGSKQ
CCCEECCCCCEEEEEEECCCHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCHHH
IKTQMTPVIFSSRVSSGLLGSFVSAISGSNLYRKNSFLLDSIGQQLFPEFVHIYEQPHLK
HHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHHHCCCCCC
GALGSAPFDGEGVPTRPNVLVEKGRLLQYVLGSYSARKMGLRTTANSDGVHNLTIDPTCG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHCCCEECCCCCCCEEEEECCCCC
DLGDLLKMMRKGLLVTELMGQGVNGITGDYSRGASGYWVEDGVIQYPVDQITIAGNLKEM
CHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCEEECCCEEECCCCEEEEECCHHHH
YRMILAVGSDINPNISMRCGSILIENMMVAGK
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]