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The map label for this gene is lysU [H]

Identifier: 148359321

GI number: 148359321

Start: 2065727

End: 2067217

Strand: Reverse

Name: lysU [H]

Synonym: LPC_1218

Alternate gene names: 148359321

Gene position: 2067217-2065727 (Counterclockwise)

Preceding gene: 148359322

Following gene: 148359315

Centisome position: 57.8

GC content: 37.76

Gene sequence:

>1491_bases
ATGAGTGAAGAACATGTACATTTAGATGAAAGTGAAGTTTATCATATTCGCAAGCAAAAATTAGCGGAATTGCGCACAGG
TGGGTTTAATTTTCCAAACACTTTTCGCCGCGAACATTTAGCCGATGCTTTGCTGAAGCAATACTCTGAAACTGAGAAAC
AAACATTAGAACAAAAGCATGTGAAAGTATCTGTTGCAGGCCGAATCGTTCTGAGAAGAATTATGGGTAAAGCCAGTTTT
TTTCATATTCAGGATGTATCAGGTCGGATACAAGTTTATCTTCGTTCAAATGATCTTCCTGACATTTATGAACAGTTTAA
ACATTGGGATTTAGGAGATATTGTTGGTGTACAGGGTGAGTTGTTTAAAACAAACACTGGTGAACTGACCATTAATGCAG
AACATGTTGAGTTGTTAACCAAATCTTTAAGGCCACTTCCTGATAAATTCCATGGTTTGGCTGATCAGGAATTGAAATAT
CGAAAACGTTATGTTGACTTGATTGCAAATGAAGACAGTCGAAAAACTTTTCTAATACGGTCACATTTAATCAAAGCCTT
CAGAGAATTTATGGATGATAATCATTTTCTGGAAGTAGAAACACCAATGATGCATCCAATTCCTGGAGGTGCTTTGGCTC
GCCCTTTCGTAACTCATCACAATACATTGGATATGACCATGTATTTGAGGATAGCCCCCGAATTGTACTTAAAACGCTTG
GTTGTTGGTGGTTTTGAGAGAGTATATGAAATTAATAGAAATTTCCGTAACGAAGGAATTTCTACTCGTCATAATCCAGA
GTTCACGATGCTGGAATTTTACCAGGCTTATGCCGATTACAATGATTTGATGAATTTTACTGAACAATTGTTTCATTATC
TATGCGATAAAGTATTAGCTACTCGGCAAATAGAATATCAAGGCCAGGTCATTGATTTCAATAAACCTTTTGAACGGTTG
AGCGTAAAAGAAGCTATATTAAAATATCATCCTGATATTAAGGCGCAGCAGTTAGAAACGGTAGAGGGATGCAGAACACT
TCTGAATGATTTAGGTCTGCCTTATAAAGAAACTGATGGCCTGGGTAAACTACAAATTATTCTTTTTGAGGAGACAGTAG
AGCACCAATTATTTCAGCCCACTTTTATTACGGAATACCCTACAGAAATATCTCCTTTAGCGAGACGTTCTGATACCAAT
CCGGAAGTCACCGATCGATTTGAGTTTTTTATTGCTGGTCGTGAGATTGCGAATGGATTTTCTGAATTAAACGATGCGGA
GGATCAAGCTGAGCGCTTTCGAAAGCAAGTTGAAGAAAAGGATGCGGGTGATTTAGAAGCAATGCATTTTGATAGTGATT
ATATTGAAGCATTGGAATATGGCTTGCCGCCTACAGCTGGTGAAGGGATTGGAATCGATCGCTTGGTGATGTTATTTACT
AATTCGCAATCAATCAGAGACGTTATTCTATTTCCGCATTTGAGACAGTAA

Upstream 100 bases:

>100_bases
ATTTGCGCACCGGTGTTGAAACCAGTAATACTCAGGCAGTATTGGATGGTTCATTAGATCAATTTATTGAAGCCAGTTTA
AAGGCAGGAGTAGGGCAAGC

Downstream 100 bases:

>100_bases
AGTACAGCGATTTTTAGATAGCAATGGTTTGTTTAGAACCATTGCTATGTCAAGCAATACATAAAATACACTTGGTTTAG
ATAAAATTATCTATGTCCAT

Product: lysine tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MSEEHVHLDESEVYHIRKQKLAELRTGGFNFPNTFRREHLADALLKQYSETEKQTLEQKHVKVSVAGRIVLRRIMGKASF
FHIQDVSGRIQVYLRSNDLPDIYEQFKHWDLGDIVGVQGELFKTNTGELTINAEHVELLTKSLRPLPDKFHGLADQELKY
RKRYVDLIANEDSRKTFLIRSHLIKAFREFMDDNHFLEVETPMMHPIPGGALARPFVTHHNTLDMTMYLRIAPELYLKRL
VVGGFERVYEINRNFRNEGISTRHNPEFTMLEFYQAYADYNDLMNFTEQLFHYLCDKVLATRQIEYQGQVIDFNKPFERL
SVKEAILKYHPDIKAQQLETVEGCRTLLNDLGLPYKETDGLGKLQIILFEETVEHQLFQPTFITEYPTEISPLARRSDTN
PEVTDRFEFFIAGREIANGFSELNDAEDQAERFRKQVEEKDAGDLEAMHFDSDYIEALEYGLPPTAGEGIGIDRLVMLFT
NSQSIRDVILFPHLRQ

Sequences:

>Translated_496_residues
MSEEHVHLDESEVYHIRKQKLAELRTGGFNFPNTFRREHLADALLKQYSETEKQTLEQKHVKVSVAGRIVLRRIMGKASF
FHIQDVSGRIQVYLRSNDLPDIYEQFKHWDLGDIVGVQGELFKTNTGELTINAEHVELLTKSLRPLPDKFHGLADQELKY
RKRYVDLIANEDSRKTFLIRSHLIKAFREFMDDNHFLEVETPMMHPIPGGALARPFVTHHNTLDMTMYLRIAPELYLKRL
VVGGFERVYEINRNFRNEGISTRHNPEFTMLEFYQAYADYNDLMNFTEQLFHYLCDKVLATRQIEYQGQVIDFNKPFERL
SVKEAILKYHPDIKAQQLETVEGCRTLLNDLGLPYKETDGLGKLQIILFEETVEHQLFQPTFITEYPTEISPLARRSDTN
PEVTDRFEFFIAGREIANGFSELNDAEDQAERFRKQVEEKDAGDLEAMHFDSDYIEALEYGLPPTAGEGIGIDRLVMLFT
NSQSIRDVILFPHLRQ
>Mature_495_residues
SEEHVHLDESEVYHIRKQKLAELRTGGFNFPNTFRREHLADALLKQYSETEKQTLEQKHVKVSVAGRIVLRRIMGKASFF
HIQDVSGRIQVYLRSNDLPDIYEQFKHWDLGDIVGVQGELFKTNTGELTINAEHVELLTKSLRPLPDKFHGLADQELKYR
KRYVDLIANEDSRKTFLIRSHLIKAFREFMDDNHFLEVETPMMHPIPGGALARPFVTHHNTLDMTMYLRIAPELYLKRLV
VGGFERVYEINRNFRNEGISTRHNPEFTMLEFYQAYADYNDLMNFTEQLFHYLCDKVLATRQIEYQGQVIDFNKPFERLS
VKEAILKYHPDIKAQQLETVEGCRTLLNDLGLPYKETDGLGKLQIILFEETVEHQLFQPTFITEYPTEISPLARRSDTNP
EVTDRFEFFIAGREIANGFSELNDAEDQAERFRKQVEEKDAGDLEAMHFDSDYIEALEYGLPPTAGEGIGIDRLVMLFTN
SQSIRDVILFPHLRQ

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=513, Percent_Identity=40.9356725146199, Blast_Score=366, Evalue=1e-101,
Organism=Homo sapiens, GI194272210, Length=513, Percent_Identity=40.9356725146199, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI45439306, Length=344, Percent_Identity=26.1627906976744, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI40789249, Length=246, Percent_Identity=24.7967479674797, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1790571, Length=481, Percent_Identity=62.5779625779626, Blast_Score=640, Evalue=0.0,
Organism=Escherichia coli, GI1789256, Length=502, Percent_Identity=60.1593625498008, Blast_Score=634, Evalue=0.0,
Organism=Escherichia coli, GI87082379, Length=332, Percent_Identity=33.1325301204819, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1788173, Length=252, Percent_Identity=26.1904761904762, Blast_Score=77, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17535925, Length=514, Percent_Identity=40.8560311284047, Blast_Score=353, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI17535927, Length=514, Percent_Identity=40.8560311284047, Blast_Score=352, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI71994340, Length=505, Percent_Identity=40.7920792079208, Blast_Score=348, Evalue=3e-96,
Organism=Caenorhabditis elegans, GI17551876, Length=338, Percent_Identity=26.9230769230769, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI32566633, Length=265, Percent_Identity=26.4150943396226, Blast_Score=82, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6320242, Length=517, Percent_Identity=42.9400386847195, Blast_Score=370, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6324256, Length=388, Percent_Identity=35.5670103092784, Blast_Score=198, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6323011, Length=347, Percent_Identity=27.0893371757925, Blast_Score=107, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6321807, Length=453, Percent_Identity=26.0485651214128, Blast_Score=95, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24640849, Length=512, Percent_Identity=39.0625, Blast_Score=354, Evalue=9e-98,
Organism=Drosophila melanogaster, GI24640851, Length=512, Percent_Identity=39.0625, Blast_Score=354, Evalue=9e-98,
Organism=Drosophila melanogaster, GI17136276, Length=465, Percent_Identity=24.3010752688172, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24584738, Length=289, Percent_Identity=28.0276816608997, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 57857; Mature: 57726

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEEHVHLDESEVYHIRKQKLAELRTGGFNFPNTFRREHLADALLKQYSETEKQTLEQKH
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKVSVAGRIVLRRIMGKASFFHIQDVSGRIQVYLRSNDLPDIYEQFKHWDLGDIVGVQGE
HHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCCHHHHHHHCCCCCHHEECCCCE
LFKTNTGELTINAEHVELLTKSLRPLPDKFHGLADQELKYRKRYVDLIANEDSRKTFLIR
EEECCCCEEEEEHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHH
SHLIKAFREFMDDNHFLEVETPMMHPIPGGALARPFVTHHNTLDMTMYLRIAPELYLKRL
HHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCHHHCCCCEEEEEEEEHHHHHHHHHH
VVGGFERVYEINRNFRNEGISTRHNPEFTMLEFYQAYADYNDLMNFTEQLFHYLCDKVLA
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
TRQIEYQGQVIDFNKPFERLSVKEAILKYHPDIKAQQLETVEGCRTLLNDLGLPYKETDG
HHEEECCCCEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
LGKLQIILFEETVEHQLFQPTFITEYPTEISPLARRSDTNPEVTDRFEFFIAGREIANGF
CCEEEEEEEHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHEEECHHHHHHH
SELNDAEDQAERFRKQVEEKDAGDLEAMHFDSDYIEALEYGLPPTAGEGIGIDRLVMLFT
HHCCCHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHCCCCCCCCCCCHHHEEEEEE
NSQSIRDVILFPHLRQ
CCCCHHHEEECCCCCC
>Mature Secondary Structure 
SEEHVHLDESEVYHIRKQKLAELRTGGFNFPNTFRREHLADALLKQYSETEKQTLEQKH
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKVSVAGRIVLRRIMGKASFFHIQDVSGRIQVYLRSNDLPDIYEQFKHWDLGDIVGVQGE
HHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCCHHHHHHHCCCCCHHEECCCCE
LFKTNTGELTINAEHVELLTKSLRPLPDKFHGLADQELKYRKRYVDLIANEDSRKTFLIR
EEECCCCEEEEEHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHH
SHLIKAFREFMDDNHFLEVETPMMHPIPGGALARPFVTHHNTLDMTMYLRIAPELYLKRL
HHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCHHHCCCCEEEEEEEEHHHHHHHHHH
VVGGFERVYEINRNFRNEGISTRHNPEFTMLEFYQAYADYNDLMNFTEQLFHYLCDKVLA
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
TRQIEYQGQVIDFNKPFERLSVKEAILKYHPDIKAQQLETVEGCRTLLNDLGLPYKETDG
HHEEECCCCEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
LGKLQIILFEETVEHQLFQPTFITEYPTEISPLARRSDTNPEVTDRFEFFIAGREIANGF
CCEEEEEEEHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHEEECHHHHHHH
SELNDAEDQAERFRKQVEEKDAGDLEAMHFDSDYIEALEYGLPPTAGEGIGIDRLVMLFT
HHCCCHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHCCCCCCCCCCCHHHEEEEEE
NSQSIRDVILFPHLRQ
CCCCHHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA