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The map label for this gene is trmJ [H]

Identifier: 148359291

GI number: 148359291

Start: 2028294

End: 2029067

Strand: Reverse

Name: trmJ [H]

Synonym: LPC_1187

Alternate gene names: 148359291

Gene position: 2029067-2028294 (Counterclockwise)

Preceding gene: 148359293

Following gene: 148359290

Centisome position: 56.73

GC content: 38.37

Gene sequence:

>774_bases
ATGAAGTTAAGTTCTATTCGAATCGTTTTGGTTTCAACTTCCCATCCTGGCAATATAGGCTCTACTGCAAGAGCTATGAA
AACCATGGGGCTAAGTACACTGTACCTGGTTAATCCAAAGTCATTTCCTGATCTCAAAGCAAAAGAAATGGCTGCTGGAG
CTGATGATGTTCTGGATGCAGCAGTTGTTACAAATACCCTGGACGAAGCTTTAATTGGATGTCAGTTGATTTTAGGGACG
AGTGCCAGACCCCGTGGACTGTCATTACCTGGATTAATTCCAGCTTCCTGTGCAGAATTGATAAATCAACAATCAGATAA
TACTCAAGTGGCCATTGTATTTGGTAGAGAACACGCAGGCCTTACTAATGAAGAGCTGTTAAAATGTCACTATCATATCA
ATATCCCAAGCAATCCTGAGTACAGTTCATTAAATTTGGCTCAGGCAGTACAAATTATCGCTTATGAACTTCGTATGAAG
TTATTATCACCAAGTGCTCAAGTAGCTCTGCGCAATGAAGAACCGGCTACAGCTGATGAAATAGAGCAATTTTATGAGCA
TTTGAAAGAAGTTTTTATTGAAATTAATTTTTTAAAGCCCTCTAATCCAAGGAGATTAATGCAAAGAGTGAGGCGACTGT
TTAATAGAATCAACCTTGAAAAAATGGAAGTTAGTATTTTAAGAGGTATGCTTAGCCAGGTTCAGAAATCATTGGAGTGG
GCAAGGAAAGACAATAGGAGCAATAAAATTGGCAATAAAACCGATTTATTTTGA

Upstream 100 bases:

>100_bases
CTATAAAATCAGCAATTCTAACACATTTTTTTTAAACAAAGAGAAGTTCAATTAATAAATCCTATGATATAGTTATCTAT
TGTATTTGGAATAATTTTTT

Downstream 100 bases:

>100_bases
TTATATGGCAACAACACCCGTTGATCCTCGCGTTGTTGAGCAAATGATAAAATATTTAGGCCCTGAAGGGGATTTTGGTA
ATCCTGCATCAGCAACTCAT

Product: RNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MKLSSIRIVLVSTSHPGNIGSTARAMKTMGLSTLYLVNPKSFPDLKAKEMAAGADDVLDAAVVTNTLDEALIGCQLILGT
SARPRGLSLPGLIPASCAELINQQSDNTQVAIVFGREHAGLTNEELLKCHYHINIPSNPEYSSLNLAQAVQIIAYELRMK
LLSPSAQVALRNEEPATADEIEQFYEHLKEVFIEINFLKPSNPRRLMQRVRRLFNRINLEKMEVSILRGMLSQVQKSLEW
ARKDNRSNKIGNKTDLF

Sequences:

>Translated_257_residues
MKLSSIRIVLVSTSHPGNIGSTARAMKTMGLSTLYLVNPKSFPDLKAKEMAAGADDVLDAAVVTNTLDEALIGCQLILGT
SARPRGLSLPGLIPASCAELINQQSDNTQVAIVFGREHAGLTNEELLKCHYHINIPSNPEYSSLNLAQAVQIIAYELRMK
LLSPSAQVALRNEEPATADEIEQFYEHLKEVFIEINFLKPSNPRRLMQRVRRLFNRINLEKMEVSILRGMLSQVQKSLEW
ARKDNRSNKIGNKTDLF
>Mature_257_residues
MKLSSIRIVLVSTSHPGNIGSTARAMKTMGLSTLYLVNPKSFPDLKAKEMAAGADDVLDAAVVTNTLDEALIGCQLILGT
SARPRGLSLPGLIPASCAELINQQSDNTQVAIVFGREHAGLTNEELLKCHYHINIPSNPEYSSLNLAQAVQIIAYELRMK
LLSPSAQVALRNEEPATADEIEQFYEHLKEVFIEINFLKPSNPRRLMQRVRRLFNRINLEKMEVSILRGMLSQVQKSLEW
ARKDNRSNKIGNKTDLF

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=250, Percent_Identity=50.4, Blast_Score=245, Evalue=2e-66,
Organism=Escherichia coli, GI1790865, Length=234, Percent_Identity=30.3418803418803, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28611; Mature: 28611

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSSIRIVLVSTSHPGNIGSTARAMKTMGLSTLYLVNPKSFPDLKAKEMAAGADDVLDA
CCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHCCHHHHHHH
AVVTNTLDEALIGCQLILGTSARPRGLSLPGLIPASCAELINQQSDNTQVAIVFGREHAG
HHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCC
LTNEELLKCHYHINIPSNPEYSSLNLAQAVQIIAYELRMKLLSPSAQVALRNEEPATADE
CCHHHEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHH
IEQFYEHLKEVFIEINFLKPSNPRRLMQRVRRLFNRINLEKMEVSILRGMLSQVQKSLEW
HHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
ARKDNRSNKIGNKTDLF
HHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MKLSSIRIVLVSTSHPGNIGSTARAMKTMGLSTLYLVNPKSFPDLKAKEMAAGADDVLDA
CCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHCCHHHHHHH
AVVTNTLDEALIGCQLILGTSARPRGLSLPGLIPASCAELINQQSDNTQVAIVFGREHAG
HHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCC
LTNEELLKCHYHINIPSNPEYSSLNLAQAVQIIAYELRMKLLSPSAQVALRNEEPATADE
CCHHHEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHH
IEQFYEHLKEVFIEINFLKPSNPRRLMQRVRRLFNRINLEKMEVSILRGMLSQVQKSLEW
HHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
ARKDNRSNKIGNKTDLF
HHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]