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The map label for this gene is rpoE [H]

Identifier: 148359113

GI number: 148359113

Start: 1810087

End: 1810650

Strand: Reverse

Name: rpoE [H]

Synonym: LPC_1001

Alternate gene names: 148359113

Gene position: 1810650-1810087 (Counterclockwise)

Preceding gene: 148359114

Following gene: 148359112

Centisome position: 50.63

GC content: 32.8

Gene sequence:

>564_bases
ATGCATATTGAGACCTCTTTAAGTGATGAATCTCTAATTGAATTAGCTCAAAAGGGAAATGGGCTAGCTTTTGATAAACT
GTTTGAGCGTTATCAGGGCAAAATTAGACAAATGATTTATTTTTATACGCATGACTGGACAAATGTCAGTGATTTAACTC
AGGAAGTTCTTATCAAAGTATTTAGACATTTATCTTATTTTAATGAGCGCAGTCAGTTTTCAACCTGGCTATATAAAATT
ACTCAAAATACTATTAAAAATTATTTTCGTACTAATAATTTACATGTTGATTCAGAAATGCAGTATATGGATGAACAATT
TTCTATACCACATAATTCTCCAGAGTCTGACATAATGGGATTAGAGTTTGAGAAACAAATAGAAGCAGCAATGTCATGTT
TATCTGAAGAGTTACGATTATGTTATGGGCTGCACATATTTGATGGACAAACTTATCAGGATATTGCAAAAGAAATGCAT
TGCCCCATCGGGACTGTACGCTCACGTATATTTAGAGCTCGAAAACAGCTGATGGATATTGTAAGTGAGAATCACACTTT
ATAA

Upstream 100 bases:

>100_bases
ATATAATAATACATAATGATTAAAACAAATCCAAAATAAATAATGATTAGACTTTATCCTATTCGAATGTAATAACAAGC
AAAGGAATAATGAGGAAGTT

Downstream 100 bases:

>100_bases
TATCATTTTGATATCATATAAAATCATCCACCTGAATCGTGAAATCAATTGCATTTAATTATTAAATAAATAAGCTTACA
AAGATCTGCTATTAGATAAA

Product: RNA polymerase sigma E factor RpoE

Products: NA

Alternate protein names: Sigma-30 [H]

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MHIETSLSDESLIELAQKGNGLAFDKLFERYQGKIRQMIYFYTHDWTNVSDLTQEVLIKVFRHLSYFNERSQFSTWLYKI
TQNTIKNYFRTNNLHVDSEMQYMDEQFSIPHNSPESDIMGLEFEKQIEAAMSCLSEELRLCYGLHIFDGQTYQDIAKEMH
CPIGTVRSRIFRARKQLMDIVSENHTL

Sequences:

>Translated_187_residues
MHIETSLSDESLIELAQKGNGLAFDKLFERYQGKIRQMIYFYTHDWTNVSDLTQEVLIKVFRHLSYFNERSQFSTWLYKI
TQNTIKNYFRTNNLHVDSEMQYMDEQFSIPHNSPESDIMGLEFEKQIEAAMSCLSEELRLCYGLHIFDGQTYQDIAKEMH
CPIGTVRSRIFRARKQLMDIVSENHTL
>Mature_187_residues
MHIETSLSDESLIELAQKGNGLAFDKLFERYQGKIRQMIYFYTHDWTNVSDLTQEVLIKVFRHLSYFNERSQFSTWLYKI
TQNTIKNYFRTNNLHVDSEMQYMDEQFSIPHNSPESDIMGLEFEKQIEAAMSCLSEELRLCYGLHIFDGQTYQDIAKEMH
CPIGTVRSRIFRARKQLMDIVSENHTL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates genes such as algD, involved in alginate biosynthesis [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=178, Percent_Identity=37.6404494382022, Blast_Score=117, Evalue=4e-28,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 22182; Mature: 22182

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIETSLSDESLIELAQKGNGLAFDKLFERYQGKIRQMIYFYTHDWTNVSDLTQEVLIKV
CCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
FRHLSYFNERSQFSTWLYKITQNTIKNYFRTNNLHVDSEMQYMDEQFSIPHNSPESDIMG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCC
LEFEKQIEAAMSCLSEELRLCYGLHIFDGQTYQDIAKEMHCPIGTVRSRIFRARKQLMDI
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
VSENHTL
HHCCCCC
>Mature Secondary Structure
MHIETSLSDESLIELAQKGNGLAFDKLFERYQGKIRQMIYFYTHDWTNVSDLTQEVLIKV
CCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
FRHLSYFNERSQFSTWLYKITQNTIKNYFRTNNLHVDSEMQYMDEQFSIPHNSPESDIMG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCC
LEFEKQIEAAMSCLSEELRLCYGLHIFDGQTYQDIAKEMHCPIGTVRSRIFRARKQLMDI
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
VSENHTL
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432708; 8378309; 7961421; 10984043; 7737518 [H]