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The map label for this gene is fixL [H]

Identifier: 148358489

GI number: 148358489

Start: 384739

End: 386154

Strand: Reverse

Name: fixL [H]

Synonym: LPC_0355

Alternate gene names: 148358489

Gene position: 386154-384739 (Counterclockwise)

Preceding gene: 148358490

Following gene: 148358488

Centisome position: 10.8

GC content: 35.81

Gene sequence:

>1416_bases
ATGACAGAAATGCATCGGTTGTTGCAGCGACAGTTAAAAAAATTTGGTTTTACTGCTGATGGATTACCACAAAATCTGGA
GAGTTGGACTCAATTTATTGCACATATCAATAAAACATACTACGAAAATGATCAAGACCGATATTTATTAGAGCGCTCTA
TTGATATTTCTTCTAAAGAAATGAGGGATATCAATTCGAAACTCGAATACGCCCAGGATATAGCTCAAATGGGGTATTGG
CATTATGACGCGGACACAGATACCGCAACATGGTCCAAAGGGCTATTTAAAATTCTGGGTTTGCCGCCTAGTAATGTAGT
GCTGAACTTGTCTTCATTTTTAGAGCTTGTGCATCCTAATGATATAGCGGGATTAAAAAAAGTGGTCGAAAAGGCTCTCA
AAGAAAAAGAAGATTATGATTATGAACTCAGAGTAAGAAACCAAGACAATGATTATAAGTGGTTCCGAACGATAGCAAGA
GCCAATCCTCATAAAAATGAAATTTCCGGGGTTTTGATGGATATCTCCAAAAGGAAAATTATTGAAGAGCAAGTTCTTGA
ACTAAATAATAAGCTGGTTTCAACAGCACGTCTTGCCGGTATGTCGGAAGTGGCCACCTCGATACTTCATAATGTGGGCA
ATATATTAAACAGCGTCAATGTTTCTGCCATTATTCTGAAAAAGAATATATCCAAAATTCATTATAAAAAACTGTCCGCT
ATAAAAACCATGCTTGACGAAAACATCAATCAATTAAGCGAATACTTGTTAAATAATCCAAAAGGTAAATTAGTTCTTCC
CTATTTGGACGCGTTATCGAAAACCCTGCATGAAGAGTATGAAGAAAACATGAAAGAAGCCAATAGTCTGGAAACTAACA
TTAATCATATTAAAGAAATCATAGCCATGCAGCAATCATTGAGCGGCTTATCGAATTTAACAGAGAAAATTTCAATCCAT
GATGTGATTCTTAATGCGGTAAAATCAGTCATATTGGACAAACATCAAATTGATGTCCGTATTGAGATTGAACCGGATAT
CAATGAGTTTATCACTGATAAATCCAAATTGTTTCAAATTCTTACTAATTTACTCGCTAATGCAAAAGATTCTGTTTTGT
TACACGAAGCGAATGCTTCCAGGACAATTGTTATTTCCATTGGGAGAACTGAAAAAAATCATATCATGATTAAAATAAAT
GATAATGGAATTGGAATTCAAAAAGATAACCTGGATCGAATTTTCTCTTTTGGATTTACAACCAAGAAAAATGGTCACGG
TTTTGGACTGCACTGTTCCGCAATTTATGCGAGAGATCTGGGTGGTACACTTTATGCTGAAAGCAATGGCAATGGCGAAG
GAGCTCAATTTGTATTAGATTTGCCCATCAATCCCAAAACCTTAAGTCCGAGGTAG

Upstream 100 bases:

>100_bases
ATCACCTCTGGCAACAGGTACCTGCGAGTTGCATAATCAAACCATGACCATTACGACCCTGTTAGAGAGTCCATAAATCA
AAAAGAGGAATTCAGTAATA

Downstream 100 bases:

>100_bases
AAATGAGTACTGAATCTTTTCGCATTCTGGTCATTGATGACAACCCGGCAATCCATCAGGATTTTATGAAAATCTTAAAT
GTCAGCAAGAACTCTGCTTT

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MTEMHRLLQRQLKKFGFTADGLPQNLESWTQFIAHINKTYYENDQDRYLLERSIDISSKEMRDINSKLEYAQDIAQMGYW
HYDADTDTATWSKGLFKILGLPPSNVVLNLSSFLELVHPNDIAGLKKVVEKALKEKEDYDYELRVRNQDNDYKWFRTIAR
ANPHKNEISGVLMDISKRKIIEEQVLELNNKLVSTARLAGMSEVATSILHNVGNILNSVNVSAIILKKNISKIHYKKLSA
IKTMLDENINQLSEYLLNNPKGKLVLPYLDALSKTLHEEYEENMKEANSLETNINHIKEIIAMQQSLSGLSNLTEKISIH
DVILNAVKSVILDKHQIDVRIEIEPDINEFITDKSKLFQILTNLLANAKDSVLLHEANASRTIVISIGRTEKNHIMIKIN
DNGIGIQKDNLDRIFSFGFTTKKNGHGFGLHCSAIYARDLGGTLYAESNGNGEGAQFVLDLPINPKTLSPR

Sequences:

>Translated_471_residues
MTEMHRLLQRQLKKFGFTADGLPQNLESWTQFIAHINKTYYENDQDRYLLERSIDISSKEMRDINSKLEYAQDIAQMGYW
HYDADTDTATWSKGLFKILGLPPSNVVLNLSSFLELVHPNDIAGLKKVVEKALKEKEDYDYELRVRNQDNDYKWFRTIAR
ANPHKNEISGVLMDISKRKIIEEQVLELNNKLVSTARLAGMSEVATSILHNVGNILNSVNVSAIILKKNISKIHYKKLSA
IKTMLDENINQLSEYLLNNPKGKLVLPYLDALSKTLHEEYEENMKEANSLETNINHIKEIIAMQQSLSGLSNLTEKISIH
DVILNAVKSVILDKHQIDVRIEIEPDINEFITDKSKLFQILTNLLANAKDSVLLHEANASRTIVISIGRTEKNHIMIKIN
DNGIGIQKDNLDRIFSFGFTTKKNGHGFGLHCSAIYARDLGGTLYAESNGNGEGAQFVLDLPINPKTLSPR
>Mature_470_residues
TEMHRLLQRQLKKFGFTADGLPQNLESWTQFIAHINKTYYENDQDRYLLERSIDISSKEMRDINSKLEYAQDIAQMGYWH
YDADTDTATWSKGLFKILGLPPSNVVLNLSSFLELVHPNDIAGLKKVVEKALKEKEDYDYELRVRNQDNDYKWFRTIARA
NPHKNEISGVLMDISKRKIIEEQVLELNNKLVSTARLAGMSEVATSILHNVGNILNSVNVSAIILKKNISKIHYKKLSAI
KTMLDENINQLSEYLLNNPKGKLVLPYLDALSKTLHEEYEENMKEANSLETNINHIKEIIAMQQSLSGLSNLTEKISIHD
VILNAVKSVILDKHQIDVRIEIEPDINEFITDKSKLFQILTNLLANAKDSVLLHEANASRTIVISIGRTEKNHIMIKIND
NGIGIQKDNLDRIFSFGFTTKKNGHGFGLHCSAIYARDLGGTLYAESNGNGEGAQFVLDLPINPKTLSPR

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=133, Percent_Identity=33.8345864661654, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 53472; Mature: 53341

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEMHRLLQRQLKKFGFTADGLPQNLESWTQFIAHINKTYYENDQDRYLLERSIDISSKE
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHH
MRDINSKLEYAQDIAQMGYWHYDADTDTATWSKGLFKILGLPPSNVVLNLSSFLELVHPN
HHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHCCCCCHHEEEHHHHHHHHCCC
DIAGLKKVVEKALKEKEDYDYELRVRNQDNDYKWFRTIARANPHKNEISGVLMDISKRKI
CHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
IEEQVLELNNKLVSTARLAGMSEVATSILHNVGNILNSVNVSAIILKKNISKIHYKKLSA
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHH
IKTMLDENINQLSEYLLNNPKGKLVLPYLDALSKTLHEEYEENMKEANSLETNINHIKEI
HHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAMQQSLSGLSNLTEKISIHDVILNAVKSVILDKHQIDVRIEIEPDINEFITDKSKLFQI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHCCHHHHHHH
LTNLLANAKDSVLLHEANASRTIVISIGRTEKNHIMIKINDNGIGIQKDNLDRIFSFGFT
HHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCCHHHHHHCCCC
TKKNGHGFGLHCSAIYARDLGGTLYAESNGNGEGAQFVLDLPINPKTLSPR
CCCCCCCCCEEEHHHHHHCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure 
TEMHRLLQRQLKKFGFTADGLPQNLESWTQFIAHINKTYYENDQDRYLLERSIDISSKE
CHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHH
MRDINSKLEYAQDIAQMGYWHYDADTDTATWSKGLFKILGLPPSNVVLNLSSFLELVHPN
HHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHCCCCCHHEEEHHHHHHHHCCC
DIAGLKKVVEKALKEKEDYDYELRVRNQDNDYKWFRTIARANPHKNEISGVLMDISKRKI
CHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
IEEQVLELNNKLVSTARLAGMSEVATSILHNVGNILNSVNVSAIILKKNISKIHYKKLSA
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHH
IKTMLDENINQLSEYLLNNPKGKLVLPYLDALSKTLHEEYEENMKEANSLETNINHIKEI
HHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAMQQSLSGLSNLTEKISIHDVILNAVKSVILDKHQIDVRIEIEPDINEFITDKSKLFQI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHCCHHHHHHH
LTNLLANAKDSVLLHEANASRTIVISIGRTEKNHIMIKINDNGIGIQKDNLDRIFSFGFT
HHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCCHHHHHHCCCC
TKKNGHGFGLHCSAIYARDLGGTLYAESNGNGEGAQFVLDLPINPKTLSPR
CCCCCCCCCEEEHHHHHHCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275; 9860942; 10747783 [H]