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The map label for this gene is bcr [H]

Identifier: 148358483

GI number: 148358483

Start: 374846

End: 376060

Strand: Reverse

Name: bcr [H]

Synonym: LPC_0349

Alternate gene names: 148358483

Gene position: 376060-374846 (Counterclockwise)

Preceding gene: 148358488

Following gene: 148358482

Centisome position: 10.51

GC content: 41.15

Gene sequence:

>1215_bases
ATGTATCCAAAAACCAAACTTTCTTTCATGACTTTATTGTTGTTAATTTCTTTTGCTTCTGTGAATGCTGTCCTATTTAC
ACCGGCATTGCCCAGCATTACTGCATTTTTCAGGATCACAGAACATGCCGCACAACAGACAATTACCAGTTTTTTGGTTG
GTTATGCCCTAGGGCAACTTGTTTATGGCCCTACAGCGAATCGCTATGGAAGGAAAAAAACACTTTATATAGGTATTGGC
TTGCAAATTATCAGCAGTCTTCTATGTGTATTGGCCGGAATGATTTCCATGTACCCGTTGCTTGTTTTAGGACGATTTCT
ATTAGCCTTAGGATCCGGTGTAGGACTGAAGATGACTTTTACTTTGGTTAATGAATGCTATGAGCCCCAATTAGCCGCGC
AAAAAACCTCCTACCTGATGCTGGCCTTTGCGATAGCACCCGGTTTGAGTGTTGCACTTGGCGGCATTTTAAACTCCCAT
TACGGCTGGGAAAGTTGTTTTTATGCAGGTACTGTTTATGGTGCTATTCTGCTTGCCATGGTCAGGCAACTTCCCGAAAC
ACAAAAAAGTCTTCAACTCGAAGCGTTTCAAATGAAACATCTGCTCCAGGGATATGTAAGTCAATTTAAAAACAAGCCAT
TAATCTCTGGTGGAGCATTAATGGGTGGCGCATCCTGCTTTGTCTACATTTTCGCCGCAACGGCACCATTTATTGCCATC
AATTTGCTAGGAATGAGTACAACAGAGTATGGCGCTGCCAATATTTTACCCTCCTTAGGCCTGACCCTCGGCTCAATCTG
GTCTGCTCAATTTGCAAAAAAATACTCGCAACGCGCGGGAATCCAATTAGGCATTTTAATCTCAGGGTTTGCCGCACTGG
CAATGATGATGGCCGCGTTAACCCATCATTCAGCGCTTATCAAGCTATTTTTTCCCATGATATTCATATATTTGGGACTA
TCTTTTATTTTTGCAAATGCTTCGACATTTGCCATGAGCCATACCTCCGATAAAGCCCATGGCTCCGCGGTTATGAACTT
CATCAATATGGGATTGGTTACAGTAGTTATTTGGGGATTAAGCTTACTTCCAATGTCACAATATTTATTACCAGCCATCT
ACATCATTCTATGTATTTTAATGATGTGGCTATTCTTTTCTTTCATAAAAAGTAATGAAATAATTTCTTCTATCGACAGG
AACAAGGAATCATGA

Upstream 100 bases:

>100_bases
TGCATTATAGTTAATAAATGTTAATTAATTATGATATTGTTAATTATTTTGTCAATAATTATACTGATCTTAATTGTTAA
TCGTTAAAACTATTCTCATC

Downstream 100 bases:

>100_bases
TGAGTATACAATTGAATCATACTATCGTATGGGTAAAAGATCGGGACAAATCAGCTCACTTCCTTACAGAAATTTTGGGG
TTGCCTGAAGCAATTCCATA

Product: major facilitator superfamily transporter transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MYPKTKLSFMTLLLLISFASVNAVLFTPALPSITAFFRITEHAAQQTITSFLVGYALGQLVYGPTANRYGRKKTLYIGIG
LQIISSLLCVLAGMISMYPLLVLGRFLLALGSGVGLKMTFTLVNECYEPQLAAQKTSYLMLAFAIAPGLSVALGGILNSH
YGWESCFYAGTVYGAILLAMVRQLPETQKSLQLEAFQMKHLLQGYVSQFKNKPLISGGALMGGASCFVYIFAATAPFIAI
NLLGMSTTEYGAANILPSLGLTLGSIWSAQFAKKYSQRAGIQLGILISGFAALAMMMAALTHHSALIKLFFPMIFIYLGL
SFIFANASTFAMSHTSDKAHGSAVMNFINMGLVTVVIWGLSLLPMSQYLLPAIYIILCILMMWLFFSFIKSNEIISSIDR
NKES

Sequences:

>Translated_404_residues
MYPKTKLSFMTLLLLISFASVNAVLFTPALPSITAFFRITEHAAQQTITSFLVGYALGQLVYGPTANRYGRKKTLYIGIG
LQIISSLLCVLAGMISMYPLLVLGRFLLALGSGVGLKMTFTLVNECYEPQLAAQKTSYLMLAFAIAPGLSVALGGILNSH
YGWESCFYAGTVYGAILLAMVRQLPETQKSLQLEAFQMKHLLQGYVSQFKNKPLISGGALMGGASCFVYIFAATAPFIAI
NLLGMSTTEYGAANILPSLGLTLGSIWSAQFAKKYSQRAGIQLGILISGFAALAMMMAALTHHSALIKLFFPMIFIYLGL
SFIFANASTFAMSHTSDKAHGSAVMNFINMGLVTVVIWGLSLLPMSQYLLPAIYIILCILMMWLFFSFIKSNEIISSIDR
NKES
>Mature_404_residues
MYPKTKLSFMTLLLLISFASVNAVLFTPALPSITAFFRITEHAAQQTITSFLVGYALGQLVYGPTANRYGRKKTLYIGIG
LQIISSLLCVLAGMISMYPLLVLGRFLLALGSGVGLKMTFTLVNECYEPQLAAQKTSYLMLAFAIAPGLSVALGGILNSH
YGWESCFYAGTVYGAILLAMVRQLPETQKSLQLEAFQMKHLLQGYVSQFKNKPLISGGALMGGASCFVYIFAATAPFIAI
NLLGMSTTEYGAANILPSLGLTLGSIWSAQFAKKYSQRAGIQLGILISGFAALAMMMAALTHHSALIKLFFPMIFIYLGL
SFIFANASTFAMSHTSDKAHGSAVMNFINMGLVTVVIWGLSLLPMSQYLLPAIYIILCILMMWLFFSFIKSNEIISSIDR
NKES

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=277, Percent_Identity=23.4657039711191, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082312, Length=253, Percent_Identity=23.7154150197628, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790146, Length=250, Percent_Identity=26.4, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI48994889, Length=338, Percent_Identity=23.3727810650888, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787065, Length=288, Percent_Identity=24.3055555555556, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43936; Mature: 43936

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYPKTKLSFMTLLLLISFASVNAVLFTPALPSITAFFRITEHAAQQTITSFLVGYALGQL
CCCCHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VYGPTANRYGRKKTLYIGIGLQIISSLLCVLAGMISMYPLLVLGRFLLALGSGVGLKMTF
HCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
TLVNECYEPQLAAQKTSYLMLAFAIAPGLSVALGGILNSHYGWESCFYAGTVYGAILLAM
HHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
VRQLPETQKSLQLEAFQMKHLLQGYVSQFKNKPLISGGALMGGASCFVYIFAATAPFIAI
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCHHHHHHHHHHHHHHHHHH
NLLGMSTTEYGAANILPSLGLTLGSIWSAQFAKKYSQRAGIQLGILISGFAALAMMMAAL
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHH
THHSALIKLFFPMIFIYLGLSFIFANASTFAMSHTSDKAHGSAVMNFINMGLVTVVIWGL
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
SLLPMSQYLLPAIYIILCILMMWLFFSFIKSNEIISSIDRNKES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MYPKTKLSFMTLLLLISFASVNAVLFTPALPSITAFFRITEHAAQQTITSFLVGYALGQL
CCCCHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VYGPTANRYGRKKTLYIGIGLQIISSLLCVLAGMISMYPLLVLGRFLLALGSGVGLKMTF
HCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
TLVNECYEPQLAAQKTSYLMLAFAIAPGLSVALGGILNSHYGWESCFYAGTVYGAILLAM
HHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
VRQLPETQKSLQLEAFQMKHLLQGYVSQFKNKPLISGGALMGGASCFVYIFAATAPFIAI
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCHHHHHHHHHHHHHHHHHH
NLLGMSTTEYGAANILPSLGLTLGSIWSAQFAKKYSQRAGIQLGILISGFAALAMMMAAL
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHH
THHSALIKLFFPMIFIYLGLSFIFANASTFAMSHTSDKAHGSAVMNFINMGLVTVVIWGL
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
SLLPMSQYLLPAIYIILCILMMWLFFSFIKSNEIISSIDRNKES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]