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The map label for this gene is pleD [H]

Identifier: 148358439

GI number: 148358439

Start: 319469

End: 321613

Strand: Reverse

Name: pleD [H]

Synonym: LPC_0305

Alternate gene names: 148358439

Gene position: 321613-319469 (Counterclockwise)

Preceding gene: 148358440

Following gene: 148358438

Centisome position: 8.99

GC content: 38.79

Gene sequence:

>2145_bases
ATGAATAACACTTGGCATCAAACCGCCACTGGAAACCGCTCCATTTCAAATTATTTGCGATTAAGCAGAACTCTCCTTAA
CGTCATCTTTATTATTGCCCTTTCCCTGCTTTTAATCATCAATATCATCTCTTATAACCAGGTCAAAAATTTACTCTTGG
CCATTAACTGGGTTTCTCAAAGCTATGAAATCATCCAAACCATTGATACTTGTCTTTACGAAGTAGTCAGCATCGAATCT
CGCCAGCGATTTTATCTGATTCGAGACTCAAACAATCAACATATGGTCGATGTGGATGAAATGAAATCCGCTTTAAAACT
GAATTTAGACAAACTCGAGAAATTAACCAGGAATAATCTGGAACAAAACAAGCGTACCAATCGTTTTACTGATTTAATTG
AGCGGAGATTAAATTTGTTAAATCAAATAGTGCAGTTAAAGGCCATTGGCAAATTCAATACGCATGAAAATTATGATTTA
CTGAATCAAAGTCAAGACATGTCTAACCAGGTTAAAAGTCTTGGGCAAGAAATCAAATCCGTTGAATTGGTGCTGTTATC
AGAAAGAAAGGCCAGTGCTGTTCGCCGGGCTGATAGAAGTAGCTTTACTTTAATATCAGGGGGCATAACAAGCATCTTGT
TTTTATTGATCGCTTTTATTCTGGCAAATAGTGAATTGTCATCGCGCGAAAAAACCGAATTGCAGAATCAGAATACGCAA
ACCCAATTAAAGAAAATCATAGAAAGCACCAGTGATATGATCGCAGCTTTTGACAAAGAACACCGATTTATTACTTTTAA
TGAGGCATATCAGCGTGAGTTTAAGCGTCTTTTTGATAAATACATAAGCATCAATCTGTCCCTGGAGAAAGCGCTCGTTG
ATATTCCTGAAAATAAAAAAGAGTTAGCTGAAATATGGAAAAATTCTTTACAAAACAATGAAGAAAATAAAACTATTGAA
TTGAATACCGAACATGAAAAAATTGTTTATGAAATGAGCTCAAAACTCATTCAAAATGGTGATAATGAAATTAATGGCGT
GGTGCACAGCGTTCGCAATATTACCAAACGGGTACAAGAACATACGGAATTACAAGAATCATATCAAAAACTGGCTAATG
GAATGAAAGAGCTGCAAGAGAAAAATGAACAAATCACCTTACTGGTTGAAATGAGTGACATTATGTTGGCTTGCAGTTCC
CAGCAAGAACTCAGTGACGTTCTGTCCAAATACTCCCAAAGACTGCTGCGATTTTCCAGTGGATATTTGTTCATCATGCA
TCCGTCCAAAAACTATTTGGAAAAAACCACCAGTTGGGGGGATCCCCAACCACACGAACTGACATTTACTCCCGAGCAAT
GCTGGGCTATCCGGCGCGGTCGAGTTCATCATGTAGACTCGTCTCATATTGAGTTATTATGTGGACATGTTTCATTTTCC
CTCGAACAGCAAGATTTATCTCTACTTTGCGTTCCTTTAATGGCCCAAAATGATATTTATGGCTTGTTATACCTGGAAGT
CAATCTAAAGCAACTCCCATTATTTGATGAGAATCAACGGTTGTTAATAACTGCATTTGCTGAGCTTACCGCATTGGCAC
TGGCAAATGTCCGCCTGGGAGAGAGTTTACGCTATCAATCTATCCGCGATCCACTCACTGGATTATACAATCGCCGTTAT
TTGGAAGATTTTCTATTTAAACAGCTACATCAAGCTGAACGGACAAAAGCTTCCTTTGCCATTTTAATGCTGGATTTGGA
TCATTTTAAAAAAATCAATGATACTTTTGGCCATGAGGCAGGAGACTTTGTATTAAAGGAGATAGGCCAAATTTTGAATA
ATGATATTCGGCTGGGTGATGTTGCAACTCGATATGGGGGTGAAGAATTCGTCCTGTTACTCTTTGACGTTGATGCCAAC
GCCGCTAAAACGAGAGCCGATAATTTGCGCTCAGCCATTTCCAAATTACAGGTTAAATACGGGGCACAATATGTTGGACA
GGTAACCGCATCAATCGGGATCTCGATATACCCTGACGATGCCAAATCCCCCGATGAGCTGATTGAAGCCGCGGATAAGG
CTTTATACCAAGCCAAAAACAAAGGCAGGAATATGGTGCTTTTATTCTCCGAAATATCATCCTAA

Upstream 100 bases:

>100_bases
GTTACGATAATCATCTTTTCGTTAATAACTGTTTTTTGGACTATACTCATATCAAGACAATGCAAAGCATCACACCATAA
AATTAGGATGATTCAATGCC

Downstream 100 bases:

>100_bases
TTTGGATAGTAGCTCCTGATTTAAATGTTGTGGTGTTTCTTATGTCCGATAGAAAGCTCCATGTGTTATACGAGTTTTGA
GAGCGAAACAATAGATACTA

Product: sensor histidine kinase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 714; Mature: 714

Protein sequence:

>714_residues
MNNTWHQTATGNRSISNYLRLSRTLLNVIFIIALSLLLIINIISYNQVKNLLLAINWVSQSYEIIQTIDTCLYEVVSIES
RQRFYLIRDSNNQHMVDVDEMKSALKLNLDKLEKLTRNNLEQNKRTNRFTDLIERRLNLLNQIVQLKAIGKFNTHENYDL
LNQSQDMSNQVKSLGQEIKSVELVLLSERKASAVRRADRSSFTLISGGITSILFLLIAFILANSELSSREKTELQNQNTQ
TQLKKIIESTSDMIAAFDKEHRFITFNEAYQREFKRLFDKYISINLSLEKALVDIPENKKELAEIWKNSLQNNEENKTIE
LNTEHEKIVYEMSSKLIQNGDNEINGVVHSVRNITKRVQEHTELQESYQKLANGMKELQEKNEQITLLVEMSDIMLACSS
QQELSDVLSKYSQRLLRFSSGYLFIMHPSKNYLEKTTSWGDPQPHELTFTPEQCWAIRRGRVHHVDSSHIELLCGHVSFS
LEQQDLSLLCVPLMAQNDIYGLLYLEVNLKQLPLFDENQRLLITAFAELTALALANVRLGESLRYQSIRDPLTGLYNRRY
LEDFLFKQLHQAERTKASFAILMLDLDHFKKINDTFGHEAGDFVLKEIGQILNNDIRLGDVATRYGGEEFVLLLFDVDAN
AAKTRADNLRSAISKLQVKYGAQYVGQVTASIGISIYPDDAKSPDELIEAADKALYQAKNKGRNMVLLFSEISS

Sequences:

>Translated_714_residues
MNNTWHQTATGNRSISNYLRLSRTLLNVIFIIALSLLLIINIISYNQVKNLLLAINWVSQSYEIIQTIDTCLYEVVSIES
RQRFYLIRDSNNQHMVDVDEMKSALKLNLDKLEKLTRNNLEQNKRTNRFTDLIERRLNLLNQIVQLKAIGKFNTHENYDL
LNQSQDMSNQVKSLGQEIKSVELVLLSERKASAVRRADRSSFTLISGGITSILFLLIAFILANSELSSREKTELQNQNTQ
TQLKKIIESTSDMIAAFDKEHRFITFNEAYQREFKRLFDKYISINLSLEKALVDIPENKKELAEIWKNSLQNNEENKTIE
LNTEHEKIVYEMSSKLIQNGDNEINGVVHSVRNITKRVQEHTELQESYQKLANGMKELQEKNEQITLLVEMSDIMLACSS
QQELSDVLSKYSQRLLRFSSGYLFIMHPSKNYLEKTTSWGDPQPHELTFTPEQCWAIRRGRVHHVDSSHIELLCGHVSFS
LEQQDLSLLCVPLMAQNDIYGLLYLEVNLKQLPLFDENQRLLITAFAELTALALANVRLGESLRYQSIRDPLTGLYNRRY
LEDFLFKQLHQAERTKASFAILMLDLDHFKKINDTFGHEAGDFVLKEIGQILNNDIRLGDVATRYGGEEFVLLLFDVDAN
AAKTRADNLRSAISKLQVKYGAQYVGQVTASIGISIYPDDAKSPDELIEAADKALYQAKNKGRNMVLLFSEISS
>Mature_714_residues
MNNTWHQTATGNRSISNYLRLSRTLLNVIFIIALSLLLIINIISYNQVKNLLLAINWVSQSYEIIQTIDTCLYEVVSIES
RQRFYLIRDSNNQHMVDVDEMKSALKLNLDKLEKLTRNNLEQNKRTNRFTDLIERRLNLLNQIVQLKAIGKFNTHENYDL
LNQSQDMSNQVKSLGQEIKSVELVLLSERKASAVRRADRSSFTLISGGITSILFLLIAFILANSELSSREKTELQNQNTQ
TQLKKIIESTSDMIAAFDKEHRFITFNEAYQREFKRLFDKYISINLSLEKALVDIPENKKELAEIWKNSLQNNEENKTIE
LNTEHEKIVYEMSSKLIQNGDNEINGVVHSVRNITKRVQEHTELQESYQKLANGMKELQEKNEQITLLVEMSDIMLACSS
QQELSDVLSKYSQRLLRFSSGYLFIMHPSKNYLEKTTSWGDPQPHELTFTPEQCWAIRRGRVHHVDSSHIELLCGHVSFS
LEQQDLSLLCVPLMAQNDIYGLLYLEVNLKQLPLFDENQRLLITAFAELTALALANVRLGESLRYQSIRDPLTGLYNRRY
LEDFLFKQLHQAERTKASFAILMLDLDHFKKINDTFGHEAGDFVLKEIGQILNNDIRLGDVATRYGGEEFVLLLFDVDAN
AAKTRADNLRSAISKLQVKYGAQYVGQVTASIGISIYPDDAKSPDELIEAADKALYQAKNKGRNMVLLFSEISS

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=190, Percent_Identity=37.3684210526316, Blast_Score=129, Evalue=7e-31,
Organism=Escherichia coli, GI145693134, Length=172, Percent_Identity=36.046511627907, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI87081881, Length=170, Percent_Identity=37.6470588235294, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1786584, Length=164, Percent_Identity=34.1463414634146, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1788381, Length=167, Percent_Identity=35.9281437125749, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI87082007, Length=170, Percent_Identity=34.7058823529412, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787541, Length=173, Percent_Identity=30.635838150289, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1788085, Length=164, Percent_Identity=32.9268292682927, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI1787816, Length=198, Percent_Identity=30.3030303030303, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI87081977, Length=226, Percent_Identity=32.3008849557522, Blast_Score=83, Evalue=8e-17,
Organism=Escherichia coli, GI1788956, Length=155, Percent_Identity=36.1290322580645, Blast_Score=83, Evalue=8e-17,
Organism=Escherichia coli, GI87081974, Length=116, Percent_Identity=37.9310344827586, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1787802, Length=160, Percent_Identity=31.25, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 82039; Mature: 82039

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNTWHQTATGNRSISNYLRLSRTLLNVIFIIALSLLLIINIISYNQVKNLLLAINWVSQ
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SYEIIQTIDTCLYEVVSIESRQRFYLIRDSNNQHMVDVDEMKSALKLNLDKLEKLTRNNL
HHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEHHHHHHHHHCCHHHHHHHHHHHH
EQNKRTNRFTDLIERRLNLLNQIVQLKAIGKFNTHENYDLLNQSQDMSNQVKSLGQEIKS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHH
VELVLLSERKASAVRRADRSSFTLISGGITSILFLLIAFILANSELSSREKTELQNQNTQ
HHHEEECCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHH
TQLKKIIESTSDMIAAFDKEHRFITFNEAYQREFKRLFDKYISINLSLEKALVDIPENKK
HHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHEECEEHHHHHHCCCCCHH
ELAEIWKNSLQNNEENKTIELNTEHEKIVYEMSSKLIQNGDNEINGVVHSVRNITKRVQE
HHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
HTELQESYQKLANGMKELQEKNEQITLLVEMSDIMLACSSQQELSDVLSKYSQRLLRFSS
HHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
GYLFIMHPSKNYLEKTTSWGDPQPHELTFTPEQCWAIRRGRVHHVDSSHIELLCGHVSFS
CEEEEECCCHHHHHHCCCCCCCCCCEEEECHHHHHHHHCCCEECCCCCCHHEEEHHHEEE
LEQQDLSLLCVPLMAQNDIYGLLYLEVNLKQLPLFDENQRLLITAFAELTALALANVRLG
ECCCCCCEEEEHHHCCCCCEEEEEEEECHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHC
ESLRYQSIRDPLTGLYNRRYLEDFLFKQLHQAERTKASFAILMLDLDHFKKINDTFGHEA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEHHHHHHHHHHHCCCH
GDFVLKEIGQILNNDIRLGDVATRYGGEEFVLLLFDVDANAAKTRADNLRSAISKLQVKY
HHHHHHHHHHHHCCCCEECHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
GAQYVGQVTASIGISIYPDDAKSPDELIEAADKALYQAKNKGRNMVLLFSEISS
HHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
>Mature Secondary Structure
MNNTWHQTATGNRSISNYLRLSRTLLNVIFIIALSLLLIINIISYNQVKNLLLAINWVSQ
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SYEIIQTIDTCLYEVVSIESRQRFYLIRDSNNQHMVDVDEMKSALKLNLDKLEKLTRNNL
HHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEHHHHHHHHHCCHHHHHHHHHHHH
EQNKRTNRFTDLIERRLNLLNQIVQLKAIGKFNTHENYDLLNQSQDMSNQVKSLGQEIKS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHH
VELVLLSERKASAVRRADRSSFTLISGGITSILFLLIAFILANSELSSREKTELQNQNTQ
HHHEEECCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHH
TQLKKIIESTSDMIAAFDKEHRFITFNEAYQREFKRLFDKYISINLSLEKALVDIPENKK
HHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHEECEEHHHHHHCCCCCHH
ELAEIWKNSLQNNEENKTIELNTEHEKIVYEMSSKLIQNGDNEINGVVHSVRNITKRVQE
HHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
HTELQESYQKLANGMKELQEKNEQITLLVEMSDIMLACSSQQELSDVLSKYSQRLLRFSS
HHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
GYLFIMHPSKNYLEKTTSWGDPQPHELTFTPEQCWAIRRGRVHHVDSSHIELLCGHVSFS
CEEEEECCCHHHHHHCCCCCCCCCCEEEECHHHHHHHHCCCEECCCCCCHHEEEHHHEEE
LEQQDLSLLCVPLMAQNDIYGLLYLEVNLKQLPLFDENQRLLITAFAELTALALANVRLG
ECCCCCCEEEEHHHCCCCCEEEEEEEECHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHC
ESLRYQSIRDPLTGLYNRRYLEDFLFKQLHQAERTKASFAILMLDLDHFKKINDTFGHEA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEHHHHHHHHHHHCCCH
GDFVLKEIGQILNNDIRLGDVATRYGGEEFVLLLFDVDANAAKTRADNLRSAISKLQVKY
HHHHHHHHHHHHCCCCEECHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
GAQYVGQVTASIGISIYPDDAKSPDELIEAADKALYQAKNKGRNMVLLFSEISS
HHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]