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The map label for this gene is prfA [C]

Identifier: 148358384

GI number: 148358384

Start: 259476

End: 259886

Strand: Reverse

Name: prfA [C]

Synonym: LPC_0248

Alternate gene names: 148358384

Gene position: 259886-259476 (Counterclockwise)

Preceding gene: 148358385

Following gene: 148358383

Centisome position: 7.27

GC content: 38.2

Gene sequence:

>411_bases
ATGATTGGCAAAGATAAATGGGAAATACTGACTGAGAAGATGACCAATCTTCATATCTATGAAACAGATCTCAGCGAAAA
ATTTATACTAGGCAGTGGTAAAGGCGGACAAAAACTACATAAAACGGCATCAACAGTCTATTTGAAACATTTGCCTACCG
GTATGGAAGTAAAATGCCAGGAATCCAGGAGTCGTGAAGATAACCGCTATTTTGCGAGGCAACGGCTTTGTGAGAAATTA
CAAGCCGTATTCAGCGATGAAAAAACAAAGACACAACAAAGAATTGAAAAGATAAAACGTCAGAAGAGACGACGCTCGAG
AAGATCCAAACAAAAAATGTTGGATGAAAAATCAAGGCAGAGTCAACTCAAGACATTAAGAAAAAACCCCCGATCTCATG
ACATTGAATAA

Upstream 100 bases:

>100_bases
TATACTCATAGTGTCTTCAAATCAAGGAAAATTATTTTGATTGAATTCAGCTATTATAATGCTTTAAGGGCATTCTTTTA
TGGGATAATAAAGAGACTTC

Downstream 100 bases:

>100_bases
TAAGCACATTAACCACTTATCATTTTAGTTAAATCAAAATACCGGGTAACATCCCTAAAGTTTTTTGTAGACTCCTGAAC
TTTAACCCAGGGACATTATT

Product: peptide chain release factor

Products: NA

Alternate protein names: Peptide Chain Release Factor; Peptidyl-TRNA Hydrolase; Peptidyl-TRNA Hydrolase-Like Protein; Peptidyl-TRNA Hydrolase Domain-Containing Protein

Number of amino acids: Translated: 136; Mature: 136

Protein sequence:

>136_residues
MIGKDKWEILTEKMTNLHIYETDLSEKFILGSGKGGQKLHKTASTVYLKHLPTGMEVKCQESRSREDNRYFARQRLCEKL
QAVFSDEKTKTQQRIEKIKRQKRRRSRRSKQKMLDEKSRQSQLKTLRKNPRSHDIE

Sequences:

>Translated_136_residues
MIGKDKWEILTEKMTNLHIYETDLSEKFILGSGKGGQKLHKTASTVYLKHLPTGMEVKCQESRSREDNRYFARQRLCEKL
QAVFSDEKTKTQQRIEKIKRQKRRRSRRSKQKMLDEKSRQSQLKTLRKNPRSHDIE
>Mature_136_residues
MIGKDKWEILTEKMTNLHIYETDLSEKFILGSGKGGQKLHKTASTVYLKHLPTGMEVKCQESRSREDNRYFARQRLCEKL
QAVFSDEKTKTQQRIEKIKRQKRRRSRRSKQKMLDEKSRQSQLKTLRKNPRSHDIE

Specific function: Peptide Chain Release Factor 1 Directs The Termination Of Translation In Response To The Peptide Chain Termination Codons Uag And Uaa. [C]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI303304971, Length=109, Percent_Identity=34.8623853211009, Blast_Score=70, Evalue=5e-13,
Organism=Homo sapiens, GI219879802, Length=109, Percent_Identity=34.8623853211009, Blast_Score=70, Evalue=5e-13,
Organism=Homo sapiens, GI22748627, Length=109, Percent_Identity=34.8623853211009, Blast_Score=70, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323311, Length=65, Percent_Identity=50.7692307692308, Blast_Score=66, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24654113, Length=100, Percent_Identity=41, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 16250; Mature: 16250

Theoretical pI: Translated: 10.89; Mature: 10.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGKDKWEILTEKMTNLHIYETDLSEKFILGSGKGGQKLHKTASTVYLKHLPTGMEVKCQ
CCCCHHHHHHHHHHHHCEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEH
ESRSREDNRYFARQRLCEKLQAVFSDEKTKTQQRIEKIKRQKRRRSRRSKQKMLDEKSRQ
HHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SQLKTLRKNPRSHDIE
HHHHHHHCCCCCCCCC
>Mature Secondary Structure
MIGKDKWEILTEKMTNLHIYETDLSEKFILGSGKGGQKLHKTASTVYLKHLPTGMEVKCQ
CCCCHHHHHHHHHHHHCEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEH
ESRSREDNRYFARQRLCEKLQAVFSDEKTKTQQRIEKIKRQKRRRSRRSKQKMLDEKSRQ
HHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SQLKTLRKNPRSHDIE
HHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA