Click here to switch to the map view.

The map label for this gene is traI [H]

Identifier: 148358325

GI number: 148358325

Start: 202055

End: 203929

Strand: Reverse

Name: traI [H]

Synonym: LPC_0187

Alternate gene names: 148358325

Gene position: 203929-202055 (Counterclockwise)

Preceding gene: 148358326

Following gene: 148358324

Centisome position: 5.7

GC content: 39.79

Gene sequence:

>1875_bases
ATGATTATTCACCATATTCCAATGAAATCAGCGCGACTAAGCAGCTTTTCTGGTCTTGTAAAATATATAACCGATCATCA
AAACAAGCAGGAACGAGTGGGAAAGGTCAGAATATCAAACTGTAACAGTGTTGACCCCACCTGGGCCGTGCAAGAAATCT
TGGCAACTCAGGCACGAAACCAAAGAGCTAAAGGCGATAAAACCTATCATTTGCTCATATCTTTTGCCCCAGGAGAAAAC
CCACCTGAAGAGGTATTAAACATTATAGAGGATAGGGTTATATCATCTGTAGGGTTTAAAGATCATCAACGAATCAGTGT
TATCCACCATGACACGGATAACCTTCATATTCATGTTGCCATAAACAAAATTCATCCAAAAACGTTTAATATGATTGAGC
CTTATAGGGCTTATAGGACTTTTGCAGAAGTCGCATCCACACTGGAAATTGAGTTTGGGCTTGAAATCACCAATCATCAA
GCACGAAAAGGCCGCTCTGAAAATCTTGCCGACGATATGGAGCAGCATTCAGGCATCGAAAGTTTAATTAACTGGATGAA
GCGAAATTGCAGAGAACAAATTGAATCGGCCAACAGTTGGAATGATGTACATAGAATCCTCACCGACCATGGTTTGGAAA
TTCTTATTAGAGCCAATGGATTTGTTTTTTGTGATAAAAAAGCACTTACGGTTAAGGCAAGCGCAGTATCCCGAAGTTTT
TCTAAAAAGAACTTAGAGGAAAGACTAGGTGAATTTATCCCCTCTCCCTTTGGCAATGAATACTATACGCAAAATGTTTA
TCGGTATGAACCATTAAACAAGAAAGTGATCGGCTCAGAAATTTATGCGCGTTATCTGTATGAAAAAGAACATAGCAAAA
CTCATCTTTCAAAAAAACTTAAACAGCTGGGGGAAGCTAAAGCCAGATTGATAGATAAAGCAAAGAAGCGCGGTCGAATC
AAGCGTAAAGCTTTGAAGTTAATGAAAGCATCGAGGAGTCAAAAAAAATATCTTTACCAGCAAATCAGCAAAACACTTCT
GAAAGATATTGAAAAAACACGCCAAAATTATGCTAAAGAACGAAAGCATCTGCTTGAATCACATCAAACAAAAATCTGGG
CTGATTGGCTGCGCCAAAAAGCAGAACTTGGAGACAAAGATGCAATAACCGCCTTGCGTTATCGCAACCGCAAAAATCAA
AGTAAATATGCATTATCAGCCGCATCATCTAATTTTTCCTTCGCTAATATTGCGCAAGTTGACTCCATTACTAAAGAAGG
AACAGAAATTTACAAATTGGATAAAGCAGTTATCCGTGATGATGGGAAAGAAATCAAAATTTCTAAAGGCGGTTCTATTG
CCACACTGAAAAAAGCGATTGAGATGGCACAGCAGAGATATGGTGACTGTATCCAAGTAAACGGTTCACCACTTTTTAAA
AAGATCATCCTTCAAATAGTTACCCAGAATAATATCCCAATTACCTTTGCTGACTCAGAGTTAGAAGCCCAGCGCCAAAA
AATGATTTCAGAACAGGAGAATCAGAATGAGCAATCAAGACGATACGGATTTAATGATGGAAGAAGAACTTCAAGAAGCA
ATGAAGCTGCTGGAGAAGCCATTGGAGAAAGTGGAAGCCATTTCAGATCGCAGCCCAACGCTGTCAGCATTAGACAAGGC
CCGCCAGCCAAAGGTCAAAACAGCCTGCGAGACTTGTCCCAACTCGATGTGGTTCAGTTCACCGGAAGAGGTAAAGTGTT
ATTGCCGGATAATGCACATGATCAGCTGGAGCGGGAAAGACTCCAACCTGATAACCATGTGCGACGGGATGTTTCTGGAT
TAAAAAAGAAAGGAAAAATCTTAAATAACAAATAA

Upstream 100 bases:

>100_bases
AAGATAGGCGTGTCGCACATTTTGATTATCGAAAAGTAAAAGCTTTATTAGATCGCATTAAAACAACGCAGGATGTTATG
TTTGAAGTGGTGAGAAAATT

Downstream 100 bases:

>100_bases
AATCAACCGTTATAACGATGAAACCTGATCAGAACTAATTCTAGTAAAATCATTAGAATACTGTCATGTATTATACTGAT
ATTATAAAATGAATTTCTTC

Product: TraI protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 624; Mature: 624

Protein sequence:

>624_residues
MIIHHIPMKSARLSSFSGLVKYITDHQNKQERVGKVRISNCNSVDPTWAVQEILATQARNQRAKGDKTYHLLISFAPGEN
PPEEVLNIIEDRVISSVGFKDHQRISVIHHDTDNLHIHVAINKIHPKTFNMIEPYRAYRTFAEVASTLEIEFGLEITNHQ
ARKGRSENLADDMEQHSGIESLINWMKRNCREQIESANSWNDVHRILTDHGLEILIRANGFVFCDKKALTVKASAVSRSF
SKKNLEERLGEFIPSPFGNEYYTQNVYRYEPLNKKVIGSEIYARYLYEKEHSKTHLSKKLKQLGEAKARLIDKAKKRGRI
KRKALKLMKASRSQKKYLYQQISKTLLKDIEKTRQNYAKERKHLLESHQTKIWADWLRQKAELGDKDAITALRYRNRKNQ
SKYALSAASSNFSFANIAQVDSITKEGTEIYKLDKAVIRDDGKEIKISKGGSIATLKKAIEMAQQRYGDCIQVNGSPLFK
KIILQIVTQNNIPITFADSELEAQRQKMISEQENQNEQSRRYGFNDGRRTSRSNEAAGEAIGESGSHFRSQPNAVSIRQG
PPAKGQNSLRDLSQLDVVQFTGRGKVLLPDNAHDQLERERLQPDNHVRRDVSGLKKKGKILNNK

Sequences:

>Translated_624_residues
MIIHHIPMKSARLSSFSGLVKYITDHQNKQERVGKVRISNCNSVDPTWAVQEILATQARNQRAKGDKTYHLLISFAPGEN
PPEEVLNIIEDRVISSVGFKDHQRISVIHHDTDNLHIHVAINKIHPKTFNMIEPYRAYRTFAEVASTLEIEFGLEITNHQ
ARKGRSENLADDMEQHSGIESLINWMKRNCREQIESANSWNDVHRILTDHGLEILIRANGFVFCDKKALTVKASAVSRSF
SKKNLEERLGEFIPSPFGNEYYTQNVYRYEPLNKKVIGSEIYARYLYEKEHSKTHLSKKLKQLGEAKARLIDKAKKRGRI
KRKALKLMKASRSQKKYLYQQISKTLLKDIEKTRQNYAKERKHLLESHQTKIWADWLRQKAELGDKDAITALRYRNRKNQ
SKYALSAASSNFSFANIAQVDSITKEGTEIYKLDKAVIRDDGKEIKISKGGSIATLKKAIEMAQQRYGDCIQVNGSPLFK
KIILQIVTQNNIPITFADSELEAQRQKMISEQENQNEQSRRYGFNDGRRTSRSNEAAGEAIGESGSHFRSQPNAVSIRQG
PPAKGQNSLRDLSQLDVVQFTGRGKVLLPDNAHDQLERERLQPDNHVRRDVSGLKKKGKILNNK
>Mature_624_residues
MIIHHIPMKSARLSSFSGLVKYITDHQNKQERVGKVRISNCNSVDPTWAVQEILATQARNQRAKGDKTYHLLISFAPGEN
PPEEVLNIIEDRVISSVGFKDHQRISVIHHDTDNLHIHVAINKIHPKTFNMIEPYRAYRTFAEVASTLEIEFGLEITNHQ
ARKGRSENLADDMEQHSGIESLINWMKRNCREQIESANSWNDVHRILTDHGLEILIRANGFVFCDKKALTVKASAVSRSF
SKKNLEERLGEFIPSPFGNEYYTQNVYRYEPLNKKVIGSEIYARYLYEKEHSKTHLSKKLKQLGEAKARLIDKAKKRGRI
KRKALKLMKASRSQKKYLYQQISKTLLKDIEKTRQNYAKERKHLLESHQTKIWADWLRQKAELGDKDAITALRYRNRKNQ
SKYALSAASSNFSFANIAQVDSITKEGTEIYKLDKAVIRDDGKEIKISKGGSIATLKKAIEMAQQRYGDCIQVNGSPLFK
KIILQIVTQNNIPITFADSELEAQRQKMISEQENQNEQSRRYGFNDGRRTSRSNEAAGEAIGESGSHFRSQPNAVSIRQG
PPAKGQNSLRDLSQLDVVQFTGRGKVLLPDNAHDQLERERLQPDNHVRRDVSGLKKKGKILNNK

Specific function: The initiation process of transfer DNA synthesis requires the interaction of at least three plasmid-specific components (traH, I, and J) at the transfer origin resulting in the assembly of a specialised nucleoprotein complex - the relaxosome. Site- and st

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005094 [H]

Pfam domain/function: PF03432 Relaxase [H]

EC number: NA

Molecular weight: Translated: 71432; Mature: 71432

Theoretical pI: Translated: 10.34; Mature: 10.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIHHIPMKSARLSSFSGLVKYITDHQNKQERVGKVRISNCNSVDPTWAVQEILATQARN
CEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHCEEEECCCCCCCHHHHHHHHHHHHHHH
QRAKGDKTYHLLISFAPGENPPEEVLNIIEDRVISSVGFKDHQRISVIHHDTDNLHIHVA
HCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEE
INKIHPKTFNMIEPYRAYRTFAEVASTLEIEFGLEITNHQARKGRSENLADDMEQHSGIE
EEEECCCCCCCCHHHHHHHHHHHHHHHEEEEECCEECCCHHHCCCCCCHHHHHHHHCCHH
SLINWMKRNCREQIESANSWNDVHRILTDHGLEILIRANGFVFCDKKALTVKASAVSRSF
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCEEEECCCEEEEEHHHHHHHH
SKKNLEERLGEFIPSPFGNEYYTQNVYRYEPLNKKVIGSEIYARYLYEKEHSKTHLSKKL
HHHHHHHHHHHHCCCCCCCCHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KQLGEAKARLIDKAKKRGRIKRKALKLMKASRSQKKYLYQQISKTLLKDIEKTRQNYAKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKHLLESHQTKIWADWLRQKAELGDKDAITALRYRNRKNQSKYALSAASSNFSFANIAQV
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHEEECCCCCCCHHHHHHH
DSITKEGTEIYKLDKAVIRDDGKEIKISKGGSIATLKKAIEMAQQRYGDCIQVNGSPLFK
HHHHHCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEECCCHHHH
KIILQIVTQNNIPITFADSELEAQRQKMISEQENQNEQSRRYGFNDGRRTSRSNEAAGEA
HHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCHHHHHH
IGESGSHFRSQPNAVSIRQGPPAKGQNSLRDLSQLDVVQFTGRGKVLLPDNAHDQLERER
HCCCCHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHEEECCCCCEECCCCCHHHHHHHH
LQPDNHVRRDVSGLKKKGKILNNK
CCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIIHHIPMKSARLSSFSGLVKYITDHQNKQERVGKVRISNCNSVDPTWAVQEILATQARN
CEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHCEEEECCCCCCCHHHHHHHHHHHHHHH
QRAKGDKTYHLLISFAPGENPPEEVLNIIEDRVISSVGFKDHQRISVIHHDTDNLHIHVA
HCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEE
INKIHPKTFNMIEPYRAYRTFAEVASTLEIEFGLEITNHQARKGRSENLADDMEQHSGIE
EEEECCCCCCCCHHHHHHHHHHHHHHHEEEEECCEECCCHHHCCCCCCHHHHHHHHCCHH
SLINWMKRNCREQIESANSWNDVHRILTDHGLEILIRANGFVFCDKKALTVKASAVSRSF
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCEEEECCCEEEEEHHHHHHHH
SKKNLEERLGEFIPSPFGNEYYTQNVYRYEPLNKKVIGSEIYARYLYEKEHSKTHLSKKL
HHHHHHHHHHHHCCCCCCCCHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KQLGEAKARLIDKAKKRGRIKRKALKLMKASRSQKKYLYQQISKTLLKDIEKTRQNYAKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKHLLESHQTKIWADWLRQKAELGDKDAITALRYRNRKNQSKYALSAASSNFSFANIAQV
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHEEECCCCCCCHHHHHHH
DSITKEGTEIYKLDKAVIRDDGKEIKISKGGSIATLKKAIEMAQQRYGDCIQVNGSPLFK
HHHHHCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEECCCHHHH
KIILQIVTQNNIPITFADSELEAQRQKMISEQENQNEQSRRYGFNDGRRTSRSNEAAGEA
HHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCHHHHHH
IGESGSHFRSQPNAVSIRQGPPAKGQNSLRDLSQLDVVQFTGRGKVLLPDNAHDQLERER
HCCCCHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHEEECCCCCEECCCCCHHHHHHHH
LQPDNHVRRDVSGLKKKGKILNNK
CCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1665997; 2168553 [H]