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The map label for this gene is pleC [H]

Identifier: 148284508

GI number: 148284508

Start: 883451

End: 884998

Strand: Direct

Name: pleC [H]

Synonym: OTBS_0907

Alternate gene names: 148284508

Gene position: 883451-884998 (Clockwise)

Preceding gene: 148284501

Following gene: 148284509

Centisome position: 41.53

GC content: 27.39

Gene sequence:

>1548_bases
GTGTATAATAATGTACTATTTAATTACAATGGGCAATATACAAAGAAATATATGCATTATAGATATGCGATTAGATTATC
ATTTATAGCTGTTTTTCTAACTTTAGTAGGCTTAACAATTTATTATAGATATAATGTGATCAAATGGTATAGCCTAATTC
CTGCACAAAAAAATGCAAGGAATATTGTTTCAAAATATAGAACCAAATTTTTAAATCAAAATCATAATAAAGATATTATT
TCTAAACTTGCGACCAATTCTTATGATTTAAAGTTGGATTTAGATTTAAAAAATTTAGCTAAACAATCTCTTAATTTCTT
TAGGGAAATTGAGTTTATGGAGTCTATAGAACAAATAACATTATATGATAATATGAAAAATATTATCATTAGATCAAAAG
ATGATTATGTTGCTACTAATAATGTTCGTAAAAATATAGGAATTGAAAGTTTGCTACTTTATCTAGATAAACTGCTTTTT
TATACATACCAGAAGCCAAATCAAAAACCTACTTTTAAACATGGAGCACGAATTACAAACTGCTTTGTTGAATTTGATAA
TAACACATATCCTTCTAGAGCTAATACGTCTCTATTTTCTGCTAATGTATTAATTCATTGTGAAAGCCCTATTATTTTAG
ACCATGCAGTAATTGCATTTTTAAAAATTACCTATAATGCTACTCAACAATGGATGTACATAAATAATATTAGCAAACAA
GTATTAACAATTCTTTTGCTAATGTCTTTAATTTTTTTCTTTATTATAAAAAGCAGTATATCTAATGTTCAGAAAATTAT
TAATGTTCAATTGAATGCCAATAAATATCTTGAACAAGCAAGGCAAAAAGCAGAAAAGGAAAATATAGCTAAAAACAAAT
TTTTAGCTAATGTTAGTCATGAACTACGAACACCGTTAAGTGCTATTATTGGACTTACAGAAATAATATTATCTAACTCT
CATTGCAAAATAAACTACTACAACTATATTCGTGATATTCATAATTCTGGAAAGCACTTATTAGCAGTAATTAATGATAT
TCTTGATTTTTCTAAGGCTTCAGCTAACAAATTAACAGTAGAAAATGTCGCAGTAGACCTTAACAAATTGGCAGCTTCAA
GTTTACGCTTGATGTATACTAAGGCCAAAAAAGCTGGTATAAAACTGATTTCTAAGTTTCCACCAGATCAAATTATTATT
AATGCAGATGCTAAACGTTTAAAACAAGCCTTGTTTAACTTGTTATCTAATTCAATAAAATTTACTCCTAGTAAAGGTTC
TATAACACTTGAAATTAGTATGAACGAACTAAAGTCTTTAGTTTATATAAAAGTTATTGATACAGGTATTGGTATTGCGG
AACAGGATATTCCTAAAGCATTATCAAGTTTTGAACAAGTAAATAATAACCTAAGTAATCAGCGTGAAGGTACAGGATTA
GGCTTGCCACTAACGAAGAAGCTCATTTACTTAATGGAAGGAGATTTCGAAATAACAAGCAATATAGGACAAGGCACAAC
TGTTACAATAACATTTAAATATCCTTAA

Upstream 100 bases:

>100_bases
ATAGAAAGACTTAGACCATAAAATATAAATTTTAGCAAATTTAAGTATATAAAATACTTATACCATATAAATGAAATTTA
AATAATACTGAAGTATTAAA

Downstream 100 bases:

>100_bases
CATTTAAATATCCTTCCAGAACAATAAATTAATACTATAATGGCAAATCTATATAGTCATTTACAATGAGGTTTGAGCAT
AGAAAAAAGCGACAATAGCA

Product: PleC-like histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MYNNVLFNYNGQYTKKYMHYRYAIRLSFIAVFLTLVGLTIYYRYNVIKWYSLIPAQKNARNIVSKYRTKFLNQNHNKDII
SKLATNSYDLKLDLDLKNLAKQSLNFFREIEFMESIEQITLYDNMKNIIIRSKDDYVATNNVRKNIGIESLLLYLDKLLF
YTYQKPNQKPTFKHGARITNCFVEFDNNTYPSRANTSLFSANVLIHCESPIILDHAVIAFLKITYNATQQWMYINNISKQ
VLTILLLMSLIFFFIIKSSISNVQKIINVQLNANKYLEQARQKAEKENIAKNKFLANVSHELRTPLSAIIGLTEIILSNS
HCKINYYNYIRDIHNSGKHLLAVINDILDFSKASANKLTVENVAVDLNKLAASSLRLMYTKAKKAGIKLISKFPPDQIII
NADAKRLKQALFNLLSNSIKFTPSKGSITLEISMNELKSLVYIKVIDTGIGIAEQDIPKALSSFEQVNNNLSNQREGTGL
GLPLTKKLIYLMEGDFEITSNIGQGTTVTITFKYP

Sequences:

>Translated_515_residues
MYNNVLFNYNGQYTKKYMHYRYAIRLSFIAVFLTLVGLTIYYRYNVIKWYSLIPAQKNARNIVSKYRTKFLNQNHNKDII
SKLATNSYDLKLDLDLKNLAKQSLNFFREIEFMESIEQITLYDNMKNIIIRSKDDYVATNNVRKNIGIESLLLYLDKLLF
YTYQKPNQKPTFKHGARITNCFVEFDNNTYPSRANTSLFSANVLIHCESPIILDHAVIAFLKITYNATQQWMYINNISKQ
VLTILLLMSLIFFFIIKSSISNVQKIINVQLNANKYLEQARQKAEKENIAKNKFLANVSHELRTPLSAIIGLTEIILSNS
HCKINYYNYIRDIHNSGKHLLAVINDILDFSKASANKLTVENVAVDLNKLAASSLRLMYTKAKKAGIKLISKFPPDQIII
NADAKRLKQALFNLLSNSIKFTPSKGSITLEISMNELKSLVYIKVIDTGIGIAEQDIPKALSSFEQVNNNLSNQREGTGL
GLPLTKKLIYLMEGDFEITSNIGQGTTVTITFKYP
>Mature_515_residues
MYNNVLFNYNGQYTKKYMHYRYAIRLSFIAVFLTLVGLTIYYRYNVIKWYSLIPAQKNARNIVSKYRTKFLNQNHNKDII
SKLATNSYDLKLDLDLKNLAKQSLNFFREIEFMESIEQITLYDNMKNIIIRSKDDYVATNNVRKNIGIESLLLYLDKLLF
YTYQKPNQKPTFKHGARITNCFVEFDNNTYPSRANTSLFSANVLIHCESPIILDHAVIAFLKITYNATQQWMYINNISKQ
VLTILLLMSLIFFFIIKSSISNVQKIINVQLNANKYLEQARQKAEKENIAKNKFLANVSHELRTPLSAIIGLTEIILSNS
HCKINYYNYIRDIHNSGKHLLAVINDILDFSKASANKLTVENVAVDLNKLAASSLRLMYTKAKKAGIKLISKFPPDQIII
NADAKRLKQALFNLLSNSIKFTPSKGSITLEISMNELKSLVYIKVIDTGIGIAEQDIPKALSSFEQVNNNLSNQREGTGL
GLPLTKKLIYLMEGDFEITSNIGQGTTVTITFKYP

Specific function: Member of the two-component regulatory system involved in the regulation of polar organelle development. PleC functions as a membrane-associated protein kinase that transfers phosphate to the response regulator pleD, leading to its activation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=258, Percent_Identity=34.4961240310078, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI48994928, Length=235, Percent_Identity=34.0425531914894, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI145693157, Length=249, Percent_Identity=34.5381526104418, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1788713, Length=239, Percent_Identity=31.7991631799163, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI87081816, Length=231, Percent_Identity=34.6320346320346, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1786783, Length=305, Percent_Identity=24.9180327868852, Blast_Score=96, Evalue=8e-21,
Organism=Escherichia coli, GI1788549, Length=234, Percent_Identity=29.4871794871795, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1790436, Length=272, Percent_Identity=27.5735294117647, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1786600, Length=218, Percent_Identity=27.9816513761468, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1788393, Length=226, Percent_Identity=25.6637168141593, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790861, Length=233, Percent_Identity=27.8969957081545, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082128, Length=298, Percent_Identity=23.1543624161074, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1790346, Length=211, Percent_Identity=25.5924170616114, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786912, Length=220, Percent_Identity=25.4545454545455, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 59018; Mature: 59018

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNNVLFNYNGQYTKKYMHYRYAIRLSFIAVFLTLVGLTIYYRYNVIKWYSLIPAQKNAR
CCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHCCCCCHHH
NIVSKYRTKFLNQNHNKDIISKLATNSYDLKLDLDLKNLAKQSLNFFREIEFMESIEQIT
HHHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
LYDNMKNIIIRSKDDYVATNNVRKNIGIESLLLYLDKLLFYTYQKPNQKPTFKHGARITN
HHCCCCCEEEECCCCEEECCCCHHHCCHHHHHHHHHHHHHHHHCCCCCCCCHHCCCEEEE
CFVEFDNNTYPSRANTSLFSANVLIHCESPIILDHAVIAFLKITYNATQQWMYINNISKQ
EEEEECCCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHEECCCCCEEEHHHHHHH
VLTILLLMSLIFFFIIKSSISNVQKIINVQLNANKYLEQARQKAEKENIAKNKFLANVSH
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELRTPLSAIIGLTEIILSNSHCKINYYNYIRDIHNSGKHLLAVINDILDFSKASANKLTV
HHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEH
ENVAVDLNKLAASSLRLMYTKAKKAGIKLISKFPPDQIIINADAKRLKQALFNLLSNSIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCEE
FTPSKGSITLEISMNELKSLVYIKVIDTGIGIAEQDIPKALSSFEQVNNNLSNQREGTGL
ECCCCCCEEEEEEHHHHHHEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
GLPLTKKLIYLMEGDFEITSNIGQGTTVTITFKYP
CCCHHHHHHHHCCCCEEEECCCCCCEEEEEEEECC
>Mature Secondary Structure
MYNNVLFNYNGQYTKKYMHYRYAIRLSFIAVFLTLVGLTIYYRYNVIKWYSLIPAQKNAR
CCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHCCCCCHHH
NIVSKYRTKFLNQNHNKDIISKLATNSYDLKLDLDLKNLAKQSLNFFREIEFMESIEQIT
HHHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
LYDNMKNIIIRSKDDYVATNNVRKNIGIESLLLYLDKLLFYTYQKPNQKPTFKHGARITN
HHCCCCCEEEECCCCEEECCCCHHHCCHHHHHHHHHHHHHHHHCCCCCCCCHHCCCEEEE
CFVEFDNNTYPSRANTSLFSANVLIHCESPIILDHAVIAFLKITYNATQQWMYINNISKQ
EEEEECCCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHEECCCCCEEEHHHHHHH
VLTILLLMSLIFFFIIKSSISNVQKIINVQLNANKYLEQARQKAEKENIAKNKFLANVSH
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELRTPLSAIIGLTEIILSNSHCKINYYNYIRDIHNSGKHLLAVINDILDFSKASANKLTV
HHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEH
ENVAVDLNKLAASSLRLMYTKAKKAGIKLISKFPPDQIIINADAKRLKQALFNLLSNSIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCEE
FTPSKGSITLEISMNELKSLVYIKVIDTGIGIAEQDIPKALSSFEQVNNNLSNQREGTGL
ECCCCCCEEEEEEHHHHHHEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
GLPLTKKLIYLMEGDFEITSNIGQGTTVTITFKYP
CCCHHHHHHHHCCCCEEEECCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8421698; 11259647 [H]