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The map label for this gene is dnaB14 [H]

Identifier: 148284509

GI number: 148284509

Start: 886020

End: 887483

Strand: Direct

Name: dnaB14 [H]

Synonym: OTBS_0911

Alternate gene names: 148284509

Gene position: 886020-887483 (Clockwise)

Preceding gene: 148284508

Following gene: 148284511

Centisome position: 41.65

GC content: 32.72

Gene sequence:

>1464_bases
ATGAAGAATATGGAAAAAGATTTACCTATTGCAAAGTCAGCTCCAAGTAATATTCAAGCTGAGCAAATGATACTTGGAGC
AGTCCTAATTAACAACCGTGTACTATATAGTATTAATGAGTTTTTACTACCTGAGCATTTTTATGAACCATTACATGGCA
AAATATACAAGTCGATTAATCTTATTATTAGTAAAGGAATTAGCGCTACTCCAATTTCGCTAAAAAATATGCTAGGTAAT
GAACCTACATTTGATGAAATTGGTGGAGTAAATTATCTTGCGAAACTTACAACTTTAGCACTAAGTATTATTAATGTAAC
TGAGTATGGCAGAATAGTATATGATCTGGCGTTACGACGTTATTTAATTGAAATTGGTGAAAAAATAGTTACAAATGCAT
ATTCTTCTACTTTAGCAGATACAGCTATCAGTCAAATAGAAGCTGCTGAATCTCAATTATATGATTTAGCGTCAATAGGA
ACTTTAAGTAAAGGATTTATCAAATTACAAACTTCAGTTGAGGAATCATGGGAATCTATTTCTGCTGCCATCAAGAATAA
AAACTCAATTAATGGTATTAGTAGTGGCTTTCTAGATCTTGATTCTAAGCTTGGAGGGTTTAAAAATTCTGATCTAATCA
TATTAGCAGGGAGACCTTCAATGGGGAAAACGGCTCTAGGAGCTAATTTAGCAGTGAATTCTTGCAAATATTTTTTATCA
TTATCTACTCAACAAAATAGCAAAGTTTCTAACATAGCACCATCAGTTGGTTTTTTTTCTTTAGAAATGTCTTCTCAGCA
AATTGCAACTCGAATCCTTGCTATAGAGTCGGAAATTGATAGTTCTTCCTTATTTAACGGGAAAATTGGTGAACAAGAGG
TTAATAAATTAAAGAATGTCCAAGATGCAATACAGAAGTGGAATTTTTACATAGATGATGCTCCTGCAATCTCAATATCA
GCAATTAGATCACGAGCTCGTAGACTTAAACGCACTCATAATTTAGCAATATTATTTATTGATTATTTGCAGTTAATTAA
GATTGATTCAAATAGAAGTCAATATAATAGAGTAAATGAAATTTCTGAAATTACTCAAAGCCTAAAAGCACTTGCAAAAG
AACTTAATATTCCAATAATTGCATTATCACAATTATCTAGAGCTGTTGAACAAAGGCCTGATAAAAAGCCGTTGCTATCA
GATCTAAGAGAATCAGGCTCCATTGAACAGGATGCAGATATTGTAATGCTCATATATAGAGAGGAATATTACTTATCTAG
ATCAGAGCCAGCTCCTGGAACGCCAGAATATACGGAATGGATTGCTAAACAAGATAGATGTCATAATACTGCTGAAATAA
TTGTTGCTAAACACCGTAATGGGCCAGTTGGTACAGTGAAGTTACACTATACTAATCAGTATTCTAAATTTGCCAATATG
GTTAAGCACTCTCTGCAAAGTTAA

Upstream 100 bases:

>100_bases
ACTTGTGTTTAAATGAGTATATCATTTTGGTTTTTGCTATAGTATTTTTAATAAGTAAAAAATAATCTAAGTTTAAGAAT
ATACAAATAATTTTAATATT

Downstream 100 bases:

>100_bases
TAAAAACCAAGATGTGAATTTAGTACTGCTAACAAAGTAATTTGAAGGATTTTTAGTAAGCAAGAGCACAATGAAAGTAA
GAAAAGAAACGTACAATTTT

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MKNMEKDLPIAKSAPSNIQAEQMILGAVLINNRVLYSINEFLLPEHFYEPLHGKIYKSINLIISKGISATPISLKNMLGN
EPTFDEIGGVNYLAKLTTLALSIINVTEYGRIVYDLALRRYLIEIGEKIVTNAYSSTLADTAISQIEAAESQLYDLASIG
TLSKGFIKLQTSVEESWESISAAIKNKNSINGISSGFLDLDSKLGGFKNSDLIILAGRPSMGKTALGANLAVNSCKYFLS
LSTQQNSKVSNIAPSVGFFSLEMSSQQIATRILAIESEIDSSSLFNGKIGEQEVNKLKNVQDAIQKWNFYIDDAPAISIS
AIRSRARRLKRTHNLAILFIDYLQLIKIDSNRSQYNRVNEISEITQSLKALAKELNIPIIALSQLSRAVEQRPDKKPLLS
DLRESGSIEQDADIVMLIYREEYYLSRSEPAPGTPEYTEWIAKQDRCHNTAEIIVAKHRNGPVGTVKLHYTNQYSKFANM
VKHSLQS

Sequences:

>Translated_487_residues
MKNMEKDLPIAKSAPSNIQAEQMILGAVLINNRVLYSINEFLLPEHFYEPLHGKIYKSINLIISKGISATPISLKNMLGN
EPTFDEIGGVNYLAKLTTLALSIINVTEYGRIVYDLALRRYLIEIGEKIVTNAYSSTLADTAISQIEAAESQLYDLASIG
TLSKGFIKLQTSVEESWESISAAIKNKNSINGISSGFLDLDSKLGGFKNSDLIILAGRPSMGKTALGANLAVNSCKYFLS
LSTQQNSKVSNIAPSVGFFSLEMSSQQIATRILAIESEIDSSSLFNGKIGEQEVNKLKNVQDAIQKWNFYIDDAPAISIS
AIRSRARRLKRTHNLAILFIDYLQLIKIDSNRSQYNRVNEISEITQSLKALAKELNIPIIALSQLSRAVEQRPDKKPLLS
DLRESGSIEQDADIVMLIYREEYYLSRSEPAPGTPEYTEWIAKQDRCHNTAEIIVAKHRNGPVGTVKLHYTNQYSKFANM
VKHSLQS
>Mature_487_residues
MKNMEKDLPIAKSAPSNIQAEQMILGAVLINNRVLYSINEFLLPEHFYEPLHGKIYKSINLIISKGISATPISLKNMLGN
EPTFDEIGGVNYLAKLTTLALSIINVTEYGRIVYDLALRRYLIEIGEKIVTNAYSSTLADTAISQIEAAESQLYDLASIG
TLSKGFIKLQTSVEESWESISAAIKNKNSINGISSGFLDLDSKLGGFKNSDLIILAGRPSMGKTALGANLAVNSCKYFLS
LSTQQNSKVSNIAPSVGFFSLEMSSQQIATRILAIESEIDSSSLFNGKIGEQEVNKLKNVQDAIQKWNFYIDDAPAISIS
AIRSRARRLKRTHNLAILFIDYLQLIKIDSNRSQYNRVNEISEITQSLKALAKELNIPIIALSQLSRAVEQRPDKKPLLS
DLRESGSIEQDADIVMLIYREEYYLSRSEPAPGTPEYTEWIAKQDRCHNTAEIIVAKHRNGPVGTVKLHYTNQYSKFANM
VKHSLQS

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=481, Percent_Identity=35.3430353430353, Blast_Score=281, Evalue=5e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 54119; Mature: 54119

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNMEKDLPIAKSAPSNIQAEQMILGAVLINNRVLYSINEFLLPEHFYEPLHGKIYKSIN
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEHHHHHCCHHHHCCHHHHHHHHHH
LIISKGISATPISLKNMLGNEPTFDEIGGVNYLAKLTTLALSIINVTEYGRIVYDLALRR
HHHHCCCCCCCHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLIEIGEKIVTNAYSSTLADTAISQIEAAESQLYDLASIGTLSKGFIKLQTSVEESWESI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHH
SAAIKNKNSINGISSGFLDLDSKLGGFKNSDLIILAGRPSMGKTALGANLAVNSCKYFLS
HHHHHCCCCCCCHHCCHHHHHHHCCCCCCCCEEEEECCCCCCCHHHCCHHHHHHHEEEEE
LSTQQNSKVSNIAPSVGFFSLEMSSQQIATRILAIESEIDSSSLFNGKIGEQEVNKLKNV
ECCCCCCCHHHCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
QDAIQKWNFYIDDAPAISISAIRSRARRLKRTHNLAILFIDYLQLIKIDSNRSQYNRVNE
HHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHEECCCHHHHHHHHH
ISEITQSLKALAKELNIPIIALSQLSRAVEQRPDKKPLLSDLRESGSIEQDADIVMLIYR
HHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEE
EEYYLSRSEPAPGTPEYTEWIAKQDRCHNTAEIIVAKHRNGPVGTVKLHYTNQYSKFANM
CHHHCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEEEECCHHHHHHHH
VKHSLQS
HHHHHCC
>Mature Secondary Structure
MKNMEKDLPIAKSAPSNIQAEQMILGAVLINNRVLYSINEFLLPEHFYEPLHGKIYKSIN
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEHHHHHCCHHHHCCHHHHHHHHHH
LIISKGISATPISLKNMLGNEPTFDEIGGVNYLAKLTTLALSIINVTEYGRIVYDLALRR
HHHHCCCCCCCHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLIEIGEKIVTNAYSSTLADTAISQIEAAESQLYDLASIGTLSKGFIKLQTSVEESWESI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHH
SAAIKNKNSINGISSGFLDLDSKLGGFKNSDLIILAGRPSMGKTALGANLAVNSCKYFLS
HHHHHCCCCCCCHHCCHHHHHHHCCCCCCCCEEEEECCCCCCCHHHCCHHHHHHHEEEEE
LSTQQNSKVSNIAPSVGFFSLEMSSQQIATRILAIESEIDSSSLFNGKIGEQEVNKLKNV
ECCCCCCCHHHCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
QDAIQKWNFYIDDAPAISISAIRSRARRLKRTHNLAILFIDYLQLIKIDSNRSQYNRVNE
HHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHEECCCHHHHHHHHH
ISEITQSLKALAKELNIPIIALSQLSRAVEQRPDKKPLLSDLRESGSIEQDADIVMLIYR
HHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEE
EEYYLSRSEPAPGTPEYTEWIAKQDRCHNTAEIIVAKHRNGPVGTVKLHYTNQYSKFANM
CHHHCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEEEECCHHHHHHHH
VKHSLQS
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA