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The map label for this gene is rnhB

Identifier: 148284488

GI number: 148284488

Start: 842936

End: 843553

Strand: Direct

Name: rnhB

Synonym: OTBS_0859

Alternate gene names: 148284488

Gene position: 842936-843553 (Clockwise)

Preceding gene: 148284484

Following gene: 148284489

Centisome position: 39.63

GC content: 32.69

Gene sequence:

>618_bases
ATGAATCCTAACTTAAATATTGAACAAGATAATGCCTCTGAAATAGTTGCTGGTGTAGATGAAGCTGGCAGAGGTCCATT
AGCTGGACCAGTAGTAGCTGCTGCTGTAGTTGTCAACCAATCAATTATGATCGATGATATTAAAGATTCAAAAGTTTTAT
CTAAATCCAAAAGAGAAAGTTGCTATGATAAGATTACTAATAGCTACTACTATAGTATTGGTATAGCATCAGTTCAAGAG
ATTGATAAATATAATATTTTAGAAGCAACAAAGCTTGCATGTATAAGAGCTGTTAAGAATTTAGCAATCATACCCACAAA
AGTGCTAGTAGATGGCAATATGAATTTTACTGACAATAGATTTATTAGTATAGTTCGTGGTGATCAGATTTGCTACTCTA
TATCATCTGCTTCAATAGTAGCAAAGGTAACAAGAGATCGCATAATGCAGATTTTACACAAAGAGTATCCAGTTTATGGT
TGGAATCAGAATTGTGGGTATGGAACTAAACTGCATATTGATGCAATTAAAACTTATGGTCAAACTATACATCATAGAGT
TAGCTTTAAATTTAAAGGCAATATCAACCATAGTGCGATATTAGGGAATATGGTATAA

Upstream 100 bases:

>100_bases
AGGTCCAGCAATTATAAATACATCATTCATTGTTAATTATAGTTTCAATAATAAGTGCACATTATAATATAATGTATACT
AAATCAATAACTTCTATAGC

Downstream 100 bases:

>100_bases
TATAGCTAAACCACTATAAAACAGTTATGTTATATTGAAAAAATGGTTTCAAGCTAAATCAAGAAGAATAAAATATCACT
GTGAGTAAACACGCTTTTCG

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MNPNLNIEQDNASEIVAGVDEAGRGPLAGPVVAAAVVVNQSIMIDDIKDSKVLSKSKRESCYDKITNSYYYSIGIASVQE
IDKYNILEATKLACIRAVKNLAIIPTKVLVDGNMNFTDNRFISIVRGDQICYSISSASIVAKVTRDRIMQILHKEYPVYG
WNQNCGYGTKLHIDAIKTYGQTIHHRVSFKFKGNINHSAILGNMV

Sequences:

>Translated_205_residues
MNPNLNIEQDNASEIVAGVDEAGRGPLAGPVVAAAVVVNQSIMIDDIKDSKVLSKSKRESCYDKITNSYYYSIGIASVQE
IDKYNILEATKLACIRAVKNLAIIPTKVLVDGNMNFTDNRFISIVRGDQICYSISSASIVAKVTRDRIMQILHKEYPVYG
WNQNCGYGTKLHIDAIKTYGQTIHHRVSFKFKGNINHSAILGNMV
>Mature_205_residues
MNPNLNIEQDNASEIVAGVDEAGRGPLAGPVVAAAVVVNQSIMIDDIKDSKVLSKSKRESCYDKITNSYYYSIGIASVQE
IDKYNILEATKLACIRAVKNLAIIPTKVLVDGNMNFTDNRFISIVRGDQICYSISSASIVAKVTRDRIMQILHKEYPVYG
WNQNCGYGTKLHIDAIKTYGQTIHHRVSFKFKGNINHSAILGNMV

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=179, Percent_Identity=45.2513966480447, Blast_Score=147, Evalue=6e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_ORITB (A5CDI4)

Other databases:

- EMBL:   AM494475
- RefSeq:   YP_001248578.1
- ProteinModelPortal:   A5CDI4
- SMR:   A5CDI4
- STRING:   A5CDI4
- GeneID:   5219446
- GenomeReviews:   AM494475_GR
- KEGG:   ots:OTBS_0859
- eggNOG:   COG0164
- HOGENOM:   HBG584843
- OMA:   IICGVDE
- ProtClustDB:   PRK00015
- BioCyc:   OTSU357244:OTBS_0859-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 22670; Mature: 22670

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPNLNIEQDNASEIVAGVDEAGRGPLAGPVVAAAVVVNQSIMIDDIKDSKVLSKSKRES
CCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHH
CYDKITNSYYYSIGIASVQEIDKYNILEATKLACIRAVKNLAIIPTKVLVDGNMNFTDNR
HHHHHCCCEEEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHCEEECEEEEEECCCCCCCCE
FISIVRGDQICYSISSASIVAKVTRDRIMQILHKEYPVYGWNQNCGYGTKLHIDAIKTYG
EEEEEECCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHH
QTIHHRVSFKFKGNINHSAILGNMV
HHHHHEEEEEEECCCCCCEECCCCC
>Mature Secondary Structure
MNPNLNIEQDNASEIVAGVDEAGRGPLAGPVVAAAVVVNQSIMIDDIKDSKVLSKSKRES
CCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHH
CYDKITNSYYYSIGIASVQEIDKYNILEATKLACIRAVKNLAIIPTKVLVDGNMNFTDNR
HHHHHCCCEEEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHCEEECEEEEEECCCCCCCCE
FISIVRGDQICYSISSASIVAKVTRDRIMQILHKEYPVYGWNQNCGYGTKLHIDAIKTYG
EEEEEECCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHH
QTIHHRVSFKFKGNINHSAILGNMV
HHHHHEEEEEEECCCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA