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The map label for this gene is nfnB [C]

Identifier: 148268956

GI number: 148268956

Start: 2694833

End: 2695504

Strand: Reverse

Name: nfnB [C]

Synonym: SaurJH9_2546

Alternate gene names: 148268956

Gene position: 2695504-2694833 (Counterclockwise)

Preceding gene: 148268958

Following gene: 148268953

Centisome position: 92.73

GC content: 36.9

Gene sequence:

>672_bases
ATGAGCAATATGAATCAAACAATTATGGATGCATTTCATTTCAGACATGCGACTAAGCAATTCGATCCACAAAAGAAAGT
TTCGAAAGAAGATTTTGAAACAATATTAGAGTCAGGTAGATTGTCTCCAATTTCTCTTGGGTTAGAACCTTGGAAGTTTG
TCGTGATTCAAGATCAAGCGTTACGTGATGAATTAAAAGCGCACAGTTGGGGCGCAGCAAAACAATTAGATACAGCGAGC
CATTTTGTGCTAATTTTTGCGCGTAAAAATGTAACGTCAAGATCACCGTATGTACAACATATGTTAAGAGATATTAAAAA
ATATGAGGCACAAACGATCCCAGCTGTTGAACAAAAATTCGATGCATTCCAAGCAGATTTCCATATTTCTGATAATGATC
AAGCCTTGTATGACTGGTCAAGTAAACAAACGTATATCGCATTAGGCAATATGATGACGACAGCCGCATTGTTAGGTATT
GATTCATGTCCGATGGAAGGTTTTAGTCTGGATACAGTGACAGACATTTTAGCAAATAAAGGTATCTTAGATACTGAGCA
ATTTGGTTTATCAGTGATGGTTGCATTTGGCTACAGACAACAAGATCCACCGAAAAATAAAACACGCCAAGCATATGAAG
ATGTTATTGAATGGGTTGGACCAAAAGAATAA

Upstream 100 bases:

>100_bases
GCACAAGTTATAGCGAATTGCAAACGGATTACTTTAGTCTTTTTAAAACATGAAGTATAATTTGTATAGTAATAAATATA
AAAATTGGAGGCTATGTTCA

Downstream 100 bases:

>100_bases
ATAGAATACAGTATGTCTAAATATATAAAATTAAAAAGTTAGCAATAAAAAAAGCCTGCGATTACATAAATGAATCGCAG
GTTTTTGCGTGAAAAAATTG

Product: nitroreductase

Products: NAD(P)(+); @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

Alternate protein names: NA

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MSNMNQTIMDAFHFRHATKQFDPQKKVSKEDFETILESGRLSPISLGLEPWKFVVIQDQALRDELKAHSWGAAKQLDTAS
HFVLIFARKNVTSRSPYVQHMLRDIKKYEAQTIPAVEQKFDAFQADFHISDNDQALYDWSSKQTYIALGNMMTTAALLGI
DSCPMEGFSLDTVTDILANKGILDTEQFGLSVMVAFGYRQQDPPKNKTRQAYEDVIEWVGPKE

Sequences:

>Translated_223_residues
MSNMNQTIMDAFHFRHATKQFDPQKKVSKEDFETILESGRLSPISLGLEPWKFVVIQDQALRDELKAHSWGAAKQLDTAS
HFVLIFARKNVTSRSPYVQHMLRDIKKYEAQTIPAVEQKFDAFQADFHISDNDQALYDWSSKQTYIALGNMMTTAALLGI
DSCPMEGFSLDTVTDILANKGILDTEQFGLSVMVAFGYRQQDPPKNKTRQAYEDVIEWVGPKE
>Mature_222_residues
SNMNQTIMDAFHFRHATKQFDPQKKVSKEDFETILESGRLSPISLGLEPWKFVVIQDQALRDELKAHSWGAAKQLDTASH
FVLIFARKNVTSRSPYVQHMLRDIKKYEAQTIPAVEQKFDAFQADFHISDNDQALYDWSSKQTYIALGNMMTTAALLGID
SCPMEGFSLDTVTDILANKGILDTEQFGLSVMVAFGYRQQDPPKNKTRQAYEDVIEWVGPKE

Specific function: Reduction Of A Variety Of Nitroaromatic Compounds Using NADH (And To Lesser Extent Nadph) As Source Of Reducing Equivalents; Two Electrons Are Transferred. Capable Of Reducing Nitrofurazone, Quinones And The Anti-Tumor Agent Cb1954 (5- (Aziridin-1-Yl)-2,4

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

Organism=Escherichia coli, GI1786792, Length=186, Percent_Identity=31.7204301075269, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 25401; Mature: 25270

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNMNQTIMDAFHFRHATKQFDPQKKVSKEDFETILESGRLSPISLGLEPWKFVVIQDQA
CCCHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHCCCCCEEEECCCCEEEEEECCHH
LRDELKAHSWGAAKQLDTASHFVLIFARKNVTSRSPYVQHMLRDIKKYEAQTIPAVEQKF
HHHHHHHCCCCCHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHH
DAFQADFHISDNDQALYDWSSKQTYIALGNMMTTAALLGIDSCPMEGFSLDTVTDILANK
HHHHCCEEECCCCCEEEECCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCC
GILDTEQFGLSVMVAFGYRQQDPPKNKTRQAYEDVIEWVGPKE
CCCCHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SNMNQTIMDAFHFRHATKQFDPQKKVSKEDFETILESGRLSPISLGLEPWKFVVIQDQA
CCHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHCCCCCEEEECCCCEEEEEECCHH
LRDELKAHSWGAAKQLDTASHFVLIFARKNVTSRSPYVQHMLRDIKKYEAQTIPAVEQKF
HHHHHHHCCCCCHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHH
DAFQADFHISDNDQALYDWSSKQTYIALGNMMTTAALLGIDSCPMEGFSLDTVTDILANK
HHHHCCEEECCCCCEEEECCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCC
GILDTEQFGLSVMVAFGYRQQDPPKNKTRQAYEDVIEWVGPKE
CCCCHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H; 6,7-Dihydropteridine [C]

Specific reaction: NAD(P)H + 6,7-Dihydropteridine = NAD(P)(+) + @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11418146 [H]