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The map label for this gene is dagK

Identifier: 148268370

GI number: 148268370

Start: 2084009

End: 2084956

Strand: Reverse

Name: dagK

Synonym: SaurJH9_1952

Alternate gene names: 148268370

Gene position: 2084956-2084009 (Counterclockwise)

Preceding gene: 148268371

Following gene: 148268369

Centisome position: 71.73

GC content: 33.76

Gene sequence:

>948_bases
ATGAGGAAACGTGCTAGAATCATTTATAACCCGACATCAGGTAAAGAGCTATTTAAAAGAGAATTACCTGATGCCTTAAT
AAAATTAGAAAAAGCGGGATATGAAACGAGTGCATATGCAACCGAGAAAATAGGTGATGCCACACTTGAAGCAGAAAGAG
CTATGCATGAAAATTATGATGTATTAATCGCTGCAGGTGGTGATGGAACATTAAATGAAGTAGTTAATGGTATCGCAGAA
AAGCCTAATCGTCCTAAGCTAGGTGTCATTCCTATGGGTACTGTTAATGACTTTGGACGTGCATTGCATATACCTAATGA
CATCATGGGGGCACTTGATGTCATCATTGAAGGTCATTCTACTAAAGTAGATATTGGTAAAATGAATAATCGATACTTTA
TTAATTTAGCTGCAGGCGGACAATTGACGCAAGTCTCTTATGAAACACCGAGTAAATTGAAATCTATTGTTGGTCCATTT
GCTTATTACATCAAAGGTTTCGAAATGTTACCTCAAATGAAAGCTGTAGATTTAAGAATTGAATATGATGGTAATGTTTT
CCAAGGAGAAGCATTATTATTCTTTTTAGGTTTAACAAATTCAATGGCAGGATTTGAAAAATTAGTGCCGGACGCTAAGT
TAGATGACGGCTATTTTACGTTAATTATAGTTGAAAAATCAAACCTTGCAGAACTTGGTCATATTATGACTTTAGCTTCA
AGAGGAGAACATACTAAGCATCCTAAAGTTATTTATGAAAAAGCAAAGGCAATTAATATTTCATCATTCACAGACTTACA
ACTAAATGTAGATGGAGAATATGGTGGTAAATTACCAGCGAATTTCTTAAATCTAGAACGTCATATAGATGTCTTTGCAC
CTAATGATATTGTAAATGAAGAGTTAATAAATAATGATCATGTTGATGACAATTTAATCGAAGAATAA

Upstream 100 bases:

>100_bases
AACGAAATAAATTTTGTTAAATATTATTTCTGTCCCACTCCCTATTAGACGAAACAAAGATGAAGTTAAAATATATGAAT
TTTAAGTAGAGGGATAAGAT

Downstream 100 bases:

>100_bases
ACAGAAATAGTGAAATATATAATGGCAGGTTAGGAAGCGCATAATTTTGTTCCTAACCTGTTCATTTTGGAGGTAAAATT
TTGCAAGCAATAGCTAAAAA

Product: lipid kinase

Products: NA

Alternate protein names: DAG kinase; DAGK

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MRKRARIIYNPTSGKELFKRELPDALIKLEKAGYETSAYATEKIGDATLEAERAMHENYDVLIAAGGDGTLNEVVNGIAE
KPNRPKLGVIPMGTVNDFGRALHIPNDIMGALDVIIEGHSTKVDIGKMNNRYFINLAAGGQLTQVSYETPSKLKSIVGPF
AYYIKGFEMLPQMKAVDLRIEYDGNVFQGEALLFFLGLTNSMAGFEKLVPDAKLDDGYFTLIIVEKSNLAELGHIMTLAS
RGEHTKHPKVIYEKAKAINISSFTDLQLNVDGEYGGKLPANFLNLERHIDVFAPNDIVNEELINNDHVDDNLIEE

Sequences:

>Translated_315_residues
MRKRARIIYNPTSGKELFKRELPDALIKLEKAGYETSAYATEKIGDATLEAERAMHENYDVLIAAGGDGTLNEVVNGIAE
KPNRPKLGVIPMGTVNDFGRALHIPNDIMGALDVIIEGHSTKVDIGKMNNRYFINLAAGGQLTQVSYETPSKLKSIVGPF
AYYIKGFEMLPQMKAVDLRIEYDGNVFQGEALLFFLGLTNSMAGFEKLVPDAKLDDGYFTLIIVEKSNLAELGHIMTLAS
RGEHTKHPKVIYEKAKAINISSFTDLQLNVDGEYGGKLPANFLNLERHIDVFAPNDIVNEELINNDHVDDNLIEE
>Mature_315_residues
MRKRARIIYNPTSGKELFKRELPDALIKLEKAGYETSAYATEKIGDATLEAERAMHENYDVLIAAGGDGTLNEVVNGIAE
KPNRPKLGVIPMGTVNDFGRALHIPNDIMGALDVIIEGHSTKVDIGKMNNRYFINLAAGGQLTQVSYETPSKLKSIVGPF
AYYIKGFEMLPQMKAVDLRIEYDGNVFQGEALLFFLGLTNSMAGFEKLVPDAKLDDGYFTLIIVEKSNLAELGHIMTLAS
RGEHTKHPKVIYEKAKAINISSFTDLQLNVDGEYGGKLPANFLNLERHIDVFAPNDIVNEELINNDHVDDNLIEE

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthet

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain

Homologues:

Organism=Escherichia coli, GI1788402, Length=264, Percent_Identity=31.0606060606061, Blast_Score=124, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAGK_STAA1 (A7X424)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001442473.1
- ProteinModelPortal:   A7X424
- SMR:   A7X424
- STRING:   A7X424
- EnsemblBacteria:   EBSTAT00000004492
- GeneID:   5560480
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_1883
- eggNOG:   COG1597
- GeneTree:   EBGT00050000024573
- HOGENOM:   HBG698136
- OMA:   FINIAAG
- ProtClustDB:   PRK13337
- BioCyc:   SAUR418127:SAHV_1883-MONOMER
- InterPro:   IPR017438
- InterPro:   IPR005218
- InterPro:   IPR001206
- Gene3D:   G3DSA:3.40.50.10330
- SMART:   SM00046
- TIGRFAMs:   TIGR00147

Pfam domain/function: PF00781 DAGK_cat

EC number: =2.7.1.107

Molecular weight: Translated: 34887; Mature: 34887

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS50146 DAGK

Important sites: ACT_SITE 273-273 BINDING 41-41 BINDING 94-94

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKRARIIYNPTSGKELFKRELPDALIKLEKAGYETSAYATEKIGDATLEAERAMHENYD
CCCCCEEEECCCCCHHHHHHHCCHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHCCCCC
VLIAAGGDGTLNEVVNGIAEKPNRPKLGVIPMGTVNDFGRALHIPNDIMGALDVIIEGHS
EEEEECCCCCHHHHHHHHHCCCCCCCEEEEECCCHHHCCCEEECCHHHHHHHHHEECCCC
TKVDIGKMNNRYFINLAAGGQLTQVSYETPSKLKSIVGPFAYYIKGFEMLPQMKAVDLRI
CEEEEECCCCEEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
EYDGNVFQGEALLFFLGLTNSMAGFEKLVPDAKLDDGYFTLIIVEKSNLAELGHIMTLAS
EECCCEEECCEEEEEEECCCCHHHHHHHCCCCEECCCEEEEEEEECCCHHHHHHHHHHHH
RGEHTKHPKVIYEKAKAINISSFTDLQLNVDGEYGGKLPANFLNLERHIDVFAPNDIVNE
CCCCCCCCHHHHHHHHEEEECCEEEEEEEECCCCCCCCCHHHHHHHHEEEEECCCHHHHH
ELINNDHVDDNLIEE
HHCCCCCCCCHHCCC
>Mature Secondary Structure
MRKRARIIYNPTSGKELFKRELPDALIKLEKAGYETSAYATEKIGDATLEAERAMHENYD
CCCCCEEEECCCCCHHHHHHHCCHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHCCCCC
VLIAAGGDGTLNEVVNGIAEKPNRPKLGVIPMGTVNDFGRALHIPNDIMGALDVIIEGHS
EEEEECCCCCHHHHHHHHHCCCCCCCEEEEECCCHHHCCCEEECCHHHHHHHHHEECCCC
TKVDIGKMNNRYFINLAAGGQLTQVSYETPSKLKSIVGPFAYYIKGFEMLPQMKAVDLRI
CEEEEECCCCEEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
EYDGNVFQGEALLFFLGLTNSMAGFEKLVPDAKLDDGYFTLIIVEKSNLAELGHIMTLAS
EECCCEEECCEEEEEEECCCCHHHHHHHCCCCEECCCEEEEEEEECCCHHHHHHHHHHHH
RGEHTKHPKVIYEKAKAINISSFTDLQLNVDGEYGGKLPANFLNLERHIDVFAPNDIVNE
CCCCCCCCHHHHHHHHEEEECCEEEEEEEECCCCCCCCCHHHHHHHHEEEEECCCHHHHH
ELINNDHVDDNLIEE
HHCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA