Click here to switch to the map view.

The map label for this gene is grpE

Identifier: 148268065

GI number: 148268065

Start: 1740195

End: 1740821

Strand: Reverse

Name: grpE

Synonym: SaurJH9_1639

Alternate gene names: 148268065

Gene position: 1740821-1740195 (Counterclockwise)

Preceding gene: 148268066

Following gene: 148268064

Centisome position: 59.89

GC content: 31.1

Gene sequence:

>627_bases
ATGACAAATAAAGACGAATCAGTTGAAAAAAACACTGAATCAACAGTTGAAGAAACAAACATCAAACAAAATATTGATGA
TTCAGTTGAACAAGCTGAAGAAAGTAAAGGTCATTTACAAGATGAAGCAATAGAAGAAACGTCTGACGAGAATGTTATTG
AAGAAATAGATCCAAAAGATCAAAAAATTAATGAACTTCAACAATTAGCAGATGAAAACGAAGAGAAATATTTAAGGCTC
TACGCTGAGTTTGAAAATTATAAGCGTAGAATTCAAAAAGAAAATGAAATAAATAAAACATATCAAGCACAACGTGTGTT
AACAGATATTTTACCAGCAATAGACAATATAGAACGTGCACTTCAAATTGAAGGTGATGATGAGACTTTTAAATCTCTTC
AAAAAGGTGTACAAATGGTGCATGAAAGTTTGATTAACGCACTAAAAGATAATGGTCTTGAAGTTATTAAAACTGAAGGT
GAAGCATTTGATCCAAATATTCACCAAGCTGTAGTTCAAGATGATAACCCTGATTTTGAATCTGGCGAAATCACTCAAGA
ACTACAAAAAGGATACAAGCTTAAAGATAGAGTATTAAGACCATCAATGGTCAAAGTAAACCAATAA

Upstream 100 bases:

>100_bases
ATCGCTGTGATTGGACCTACAGCTATGCATTATCAAAATGTCATTCAATTATTAAATAGAATTTGGTAATGATGGATTAG
AAAGTATTGGAGGACAGAAA

Downstream 100 bases:

>100_bases
CTTAAATTTGGCGAAAAGACATTGTTTAAAATTAAATTAATTTAATGATTAAATGGAGGAATTTTATTATGAGTAAAATT
ATTGGTATAGACTTAGGTAC

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MTNKDESVEKNTESTVEETNIKQNIDDSVEQAEESKGHLQDEAIEETSDENVIEEIDPKDQKINELQQLADENEEKYLRL
YAEFENYKRRIQKENEINKTYQAQRVLTDILPAIDNIERALQIEGDDETFKSLQKGVQMVHESLINALKDNGLEVIKTEG
EAFDPNIHQAVVQDDNPDFESGEITQELQKGYKLKDRVLRPSMVKVNQ

Sequences:

>Translated_208_residues
MTNKDESVEKNTESTVEETNIKQNIDDSVEQAEESKGHLQDEAIEETSDENVIEEIDPKDQKINELQQLADENEEKYLRL
YAEFENYKRRIQKENEINKTYQAQRVLTDILPAIDNIERALQIEGDDETFKSLQKGVQMVHESLINALKDNGLEVIKTEG
EAFDPNIHQAVVQDDNPDFESGEITQELQKGYKLKDRVLRPSMVKVNQ
>Mature_207_residues
TNKDESVEKNTESTVEETNIKQNIDDSVEQAEESKGHLQDEAIEETSDENVIEEIDPKDQKINELQQLADENEEKYLRLY
AEFENYKRRIQKENEINKTYQAQRVLTDILPAIDNIERALQIEGDDETFKSLQKGVQMVHESLINALKDNGLEVIKTEGE
AFDPNIHQAVVQDDNPDFESGEITQELQKGYKLKDRVLRPSMVKVNQ

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=199, Percent_Identity=29.1457286432161, Blast_Score=68, Evalue=5e-12,
Organism=Escherichia coli, GI1788967, Length=172, Percent_Identity=31.3953488372093, Blast_Score=99, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17552458, Length=151, Percent_Identity=31.1258278145695, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324806, Length=142, Percent_Identity=33.0985915492958, Blast_Score=80, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24653432, Length=172, Percent_Identity=29.0697674418605, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_STAA1 (A7X2Y2)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001442158.1
- ProteinModelPortal:   A7X2Y2
- SMR:   A7X2Y2
- STRING:   A7X2Y2
- EnsemblBacteria:   EBSTAT00000005116
- GeneID:   5560967
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_1568
- eggNOG:   COG0576
- GeneTree:   EBGT00050000023992
- HOGENOM:   HBG732630
- OMA:   NLHQAVM
- ProtClustDB:   PRK14140
- BioCyc:   SAUR418127:SAHV_1568-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 24023; Mature: 23891

Theoretical pI: Translated: 4.14; Mature: 4.14

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNKDESVEKNTESTVEETNIKQNIDDSVEQAEESKGHLQDEAIEETSDENVIEEIDPKD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHCCCCH
QKINELQQLADENEEKYLRLYAEFENYKRRIQKENEINKTYQAQRVLTDILPAIDNIERA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQIEGDDETFKSLQKGVQMVHESLINALKDNGLEVIKTEGEAFDPNIHQAVVQDDNPDFE
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHCCCCCCCC
SGEITQELQKGYKLKDRVLRPSMVKVNQ
CCHHHHHHHHCCHHHHHHCCCCHHCCCC
>Mature Secondary Structure 
TNKDESVEKNTESTVEETNIKQNIDDSVEQAEESKGHLQDEAIEETSDENVIEEIDPKD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHCCCCH
QKINELQQLADENEEKYLRLYAEFENYKRRIQKENEINKTYQAQRVLTDILPAIDNIERA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQIEGDDETFKSLQKGVQMVHESLINALKDNGLEVIKTEGEAFDPNIHQAVVQDDNPDFE
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHCCCCCCCC
SGEITQELQKGYKLKDRVLRPSMVKVNQ
CCHHHHHHHHCCHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA