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The map label for this gene is pdhC [H]

Identifier: 148267588

GI number: 148267588

Start: 1197604

End: 1198896

Strand: Direct

Name: pdhC [H]

Synonym: SaurJH9_1155

Alternate gene names: 148267588

Gene position: 1197604-1198896 (Clockwise)

Preceding gene: 148267587

Following gene: 148267589

Centisome position: 41.2

GC content: 38.05

Gene sequence:

>1293_bases
GTGGCATTTGAATTTAGATTACCCGATATCGGGGAAGGTATCCACGAAGGTGAAATTGTAAAATGGTTTGTTAAAGCTGG
AGATACTATTGAAGAAGACGATGTTTTAGCTGAGGTACAAAACGATAAATCAGTAGTAGAAATCCCATCACCAGTATCTG
GTACTGTAGAAGAAGTTATGGTAGAAGAAGGTACAGTAGCTGTAGTTGGTGACGTTATTGTTAAAATCGATGCACCTGAT
GCAGAAGATATGCAATTTAAAGGTCATGATGATGATTCATCATCTAAAGAAGAACCTGCGAAAGAGGAAGCGCCAGCAGA
GCAAGCACCTGTAGCTACTCAAACTGAAGAAGTAGATGAAAACAGAACTGTTAAAGCAATGCCTTCAGTACGTAAATACG
CACGTGAAAAAGGTGTTAACATTAAAGCAGTTTCTGGATCTGGTAAAAATGGTCGTATTACAAAAGAAGATGTAGATGCA
TACTTAAATGGTGGTGCACCAACAGCTTCAAATGAATCAGCTGCTTCAGCTACAAGTGAAGAAGTTGCTGAAACTCCTGC
AGCACCTGCAGCAGTATCATTAGAAGGCGACTTCCCAGAAACAACTGAAAAAATCCCTGCTATGCGTAGAGCAATTGCGA
AAGCAATGGTTAACTCTAAGCATACTGCACCTCATGTAACATTAATGGATGAAATTGATGTTCAAGCATTATGGGATCAC
CGTAAGAAATTTAAAGAAATCGCAGCTGAACAAGGTACTAAGTTAACATTCTTACCTTATGTTGTTAAAGCACTTGTTTC
TGCATTGAAAAAATACCCAGCACTTAACACTTCATTCAATGAAGAAGCTGGTGAAATTGTGCATAAACATTATTGGAATA
TCGGTATTGCGGCGGACACTGATAGAGGTTTATTAGTACCTGTAGTGAAACATGCTGATCGTAAGTCTATTTTCCAAATT
TCAGATGAAATTAATGAATTAGCTGTTAAAGCACGTGATGGTAAATTAACAGCCGATGAAATGAAAGGTGCAACATGCAC
AATCAGTAATATCGGTTCAGCTGGTGGACAATGGTTCACTCCAGTTATCAATCACCCAGAAGTAGCAATCTTAGGAATTG
GCCGTATTGCTCAAAAACCTATCGTTAAAGATGGAGAAATTGTTGCAGCACCAGTATTAGCATTATCATTAAGCTTTGAC
CACAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATT
AATGGAGGGGTAA

Upstream 100 bases:

>100_bases
AGAATTTTAATACATTTTAAAAGTTAACGAAGTTAGCGTATTTTAGTCTCATTGATTAAAATGAAATGTTTAATTTACGA
AATCTTAGGAGGGCAAAAAC

Downstream 100 bases:

>100_bases
AACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAAT
TCGTGCAGCTCAATTAGGAC

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MAFEFRLPDIGEGIHEGEIVKWFVKAGDTIEEDDVLAEVQNDKSVVEIPSPVSGTVEEVMVEEGTVAVVGDVIVKIDAPD
AEDMQFKGHDDDSSSKEEPAKEEAPAEQAPVATQTEEVDENRTVKAMPSVRKYAREKGVNIKAVSGSGKNGRITKEDVDA
YLNGGAPTASNESAASATSEEVAETPAAPAAVSLEGDFPETTEKIPAMRRAIAKAMVNSKHTAPHVTLMDEIDVQALWDH
RKKFKEIAAEQGTKLTFLPYVVKALVSALKKYPALNTSFNEEAGEIVHKHYWNIGIAADTDRGLLVPVVKHADRKSIFQI
SDEINELAVKARDGKLTADEMKGATCTISNIGSAGGQWFTPVINHPEVAILGIGRIAQKPIVKDGEIVAAPVLALSLSFD
HRQIDGATGQNAMNHIKRLLNNPELLLMEG

Sequences:

>Translated_430_residues
MAFEFRLPDIGEGIHEGEIVKWFVKAGDTIEEDDVLAEVQNDKSVVEIPSPVSGTVEEVMVEEGTVAVVGDVIVKIDAPD
AEDMQFKGHDDDSSSKEEPAKEEAPAEQAPVATQTEEVDENRTVKAMPSVRKYAREKGVNIKAVSGSGKNGRITKEDVDA
YLNGGAPTASNESAASATSEEVAETPAAPAAVSLEGDFPETTEKIPAMRRAIAKAMVNSKHTAPHVTLMDEIDVQALWDH
RKKFKEIAAEQGTKLTFLPYVVKALVSALKKYPALNTSFNEEAGEIVHKHYWNIGIAADTDRGLLVPVVKHADRKSIFQI
SDEINELAVKARDGKLTADEMKGATCTISNIGSAGGQWFTPVINHPEVAILGIGRIAQKPIVKDGEIVAAPVLALSLSFD
HRQIDGATGQNAMNHIKRLLNNPELLLMEG
>Mature_429_residues
AFEFRLPDIGEGIHEGEIVKWFVKAGDTIEEDDVLAEVQNDKSVVEIPSPVSGTVEEVMVEEGTVAVVGDVIVKIDAPDA
EDMQFKGHDDDSSSKEEPAKEEAPAEQAPVATQTEEVDENRTVKAMPSVRKYAREKGVNIKAVSGSGKNGRITKEDVDAY
LNGGAPTASNESAASATSEEVAETPAAPAAVSLEGDFPETTEKIPAMRRAIAKAMVNSKHTAPHVTLMDEIDVQALWDHR
KKFKEIAAEQGTKLTFLPYVVKALVSALKKYPALNTSFNEEAGEIVHKHYWNIGIAADTDRGLLVPVVKHADRKSIFQIS
DEINELAVKARDGKLTADEMKGATCTISNIGSAGGQWFTPVINHPEVAILGIGRIAQKPIVKDGEIVAAPVLALSLSFDH
RQIDGATGQNAMNHIKRLLNNPELLLMEG

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=431, Percent_Identity=36.4269141531323, Blast_Score=237, Evalue=2e-62,
Organism=Homo sapiens, GI31711992, Length=445, Percent_Identity=28.9887640449438, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI203098753, Length=452, Percent_Identity=27.212389380531, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI203098816, Length=452, Percent_Identity=27.212389380531, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI19923748, Length=255, Percent_Identity=34.1176470588235, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI260898739, Length=167, Percent_Identity=31.7365269461078, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1786946, Length=429, Percent_Identity=30.5361305361305, Blast_Score=224, Evalue=8e-60,
Organism=Escherichia coli, GI1786305, Length=429, Percent_Identity=35.4312354312354, Blast_Score=221, Evalue=6e-59,
Organism=Caenorhabditis elegans, GI17537937, Length=433, Percent_Identity=33.7182448036951, Blast_Score=232, Evalue=3e-61,
Organism=Caenorhabditis elegans, GI25146366, Length=430, Percent_Identity=30.9302325581395, Blast_Score=191, Evalue=7e-49,
Organism=Caenorhabditis elegans, GI17560088, Length=449, Percent_Identity=30.5122494432071, Blast_Score=178, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI17538894, Length=308, Percent_Identity=27.5974025974026, Blast_Score=107, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6320352, Length=425, Percent_Identity=31.0588235294118, Blast_Score=202, Evalue=7e-53,
Organism=Saccharomyces cerevisiae, GI6324258, Length=450, Percent_Identity=30, Blast_Score=166, Evalue=9e-42,
Organism=Drosophila melanogaster, GI18859875, Length=427, Percent_Identity=34.6604215456674, Blast_Score=235, Evalue=5e-62,
Organism=Drosophila melanogaster, GI24645909, Length=245, Percent_Identity=32.2448979591837, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24582497, Length=233, Percent_Identity=32.618025751073, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI20129315, Length=233, Percent_Identity=32.618025751073, Blast_Score=126, Evalue=2e-29,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 46369; Mature: 46238

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFEFRLPDIGEGIHEGEIVKWFVKAGDTIEEDDVLAEVQNDKSVVEIPSPVSGTVEEVM
CCEEECCCHHCCCCCCCHHEEEEECCCCCCCCCHHHHHHCCCCCEEECCCCCCCCHHHHH
VEEGTVAVVGDVIVKIDAPDAEDMQFKGHDDDSSSKEEPAKEEAPAEQAPVATQTEEVDE
HCCCCEEEEEEEEEEECCCCCCCCEECCCCCCCCCCCCCCHHHCCCHHCCCCCCHHHHCC
NRTVKAMPSVRKYAREKGVNIKAVSGSGKNGRITKEDVDAYLNGGAPTASNESAASATSE
CCCEEHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCHHHCCHH
EVAETPAAPAAVSLEGDFPETTEKIPAMRRAIAKAMVNSKHTAPHVTLMDEIDVQALWDH
HHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHH
RKKFKEIAAEQGTKLTFLPYVVKALVSALKKYPALNTSFNEEAGEIVHKHYWNIGIAADT
HHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCEEEEEECC
DRGLLVPVVKHADRKSIFQISDEINELAVKARDGKLTADEMKGATCTISNIGSAGGQWFT
CCCEEEHHHHCCCHHHHHHHHHHHHHHEEEECCCCEEHHHHCCCEEEECCCCCCCCCEEC
PVINHPEVAILGIGRIAQKPIVKDGEIVAAPVLALSLSFDHRQIDGATGQNAMNHIKRLL
CCCCCCCEEEEECCHHHCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHH
NNPELLLMEG
CCCCEEEECC
>Mature Secondary Structure 
AFEFRLPDIGEGIHEGEIVKWFVKAGDTIEEDDVLAEVQNDKSVVEIPSPVSGTVEEVM
CEEECCCHHCCCCCCCHHEEEEECCCCCCCCCHHHHHHCCCCCEEECCCCCCCCHHHHH
VEEGTVAVVGDVIVKIDAPDAEDMQFKGHDDDSSSKEEPAKEEAPAEQAPVATQTEEVDE
HCCCCEEEEEEEEEEECCCCCCCCEECCCCCCCCCCCCCCHHHCCCHHCCCCCCHHHHCC
NRTVKAMPSVRKYAREKGVNIKAVSGSGKNGRITKEDVDAYLNGGAPTASNESAASATSE
CCCEEHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCHHHCCHH
EVAETPAAPAAVSLEGDFPETTEKIPAMRRAIAKAMVNSKHTAPHVTLMDEIDVQALWDH
HHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHH
RKKFKEIAAEQGTKLTFLPYVVKALVSALKKYPALNTSFNEEAGEIVHKHYWNIGIAADT
HHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCEEEEEECC
DRGLLVPVVKHADRKSIFQISDEINELAVKARDGKLTADEMKGATCTISNIGSAGGQWFT
CCCEEEHHHHCCCHHHHHHHHHHHHHHEEEECCCCEEHHHHCCCEEEECCCCCCCCCEEC
PVINHPEVAILGIGRIAQKPIVKDGEIVAAPVLALSLSFDHRQIDGATGQNAMNHIKRLL
CCCCCCCEEEEECCHHHCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHH
NNPELLLMEG
CCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA