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The map label for this gene is pdhD

Identifier: 148267589

GI number: 148267589

Start: 1198900

End: 1200306

Strand: Direct

Name: pdhD

Synonym: SaurJH9_1156

Alternate gene names: 148267589

Gene position: 1198900-1200306 (Clockwise)

Preceding gene: 148267588

Following gene: 148267590

Centisome position: 41.25

GC content: 36.96

Gene sequence:

>1407_bases
ATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCG
TGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTC
CTTCAAAAGCATTACTACATGCTTCTCACCGTTTTGTTGAAGCACAACATTCTGAAAACTTAGGTGTTATTGCTGAAAGT
GTTTCTTTAAACTTCCAAAAAGTTCAAGAATTCAAATCATCAGTTGTTAATAAATTAACTGGTGGTGTTGAAGGCTTACT
TAAAGGTAACAAAGTTAACATCGTTAAAGGTGAAGCATATTTCGTAGATAACAATAGCTTACGTGTTATGGACGAAAAGA
GCGCACAAACATACAACTTTAAAAATGCAATCATTGCAACAGGTTCAAGACCAATTGAAATTCCTAATTTCAAATTCGGT
AAACGTGTTATCGACTCAACAGGTGCTTTAAACTTACAAGAAGTACCAGGTAAATTAGTTGTAGTTGGTGGAGGATACAT
TGGATCAGAATTAGGTACAGCATTTGCTAACTTTGGTTCAGAAGTAACCATCCTTGAAGGTGCTAAAGATATCTTAGGTG
GCTTCGAAAAACAAATGACACAACCTGTTAAAAAAGGTATGAAAGAAAAAGGTGTTGAAATCGTTACTGAAGCTATGGCT
AAATCAGCTGAAGAAACAGATAACGGAGTTAAAGTTACTTATGAAGCTAAAGGCGAAGAGAAAACAATCGAAGCTGATTA
TGTATTAGTAACTGTAGGTCGTCGTCCAAACACAGACGAATTAGGCCTAGAAGAATTAGGTGTTAAATTCGCTGACCGTG
GATTATTAGAAGTTGATAAACAAAGCCGTACGTCTATCAGCAATATCTATGCAATTGGTGATATCGTTCCAGGTTTACCA
CTTGCTCACAAAGCTAGCTATGAAGCTAAAGTTGCTGCTGAAGCAATTGATGGTCAAGCTGCTGAAGTTGATTACATTGG
TATGCCAGCAGTATGCTTTACTGAACCAGAATTAGCTACAGTTGGTTATTCAGAAGCGCAAGCTAAAGAAGAAGGTTTAG
CAATTAAAGCTTCTAAATTCCCATATGCAGCAAATGGTCGTGCATTATCATTAGATGATACTAACGGATTTGTTAAACTT
ATTACACTTAAAGAAGATGATACTTTAATCGGTGCTCAAGTAGTTGGTACTGGTGCATCAGATATTATCTCTGAATTAGG
TTTAGCAATTGAAGCTGGTATGAATGCTGAAGATATCGCATTAACAATCCATGCACATCCAACATTAGGTGAGATGACTA
TGGAAGCAGCAGAAAAAGCTATCGGATACCCAATCCATACAATGTAA

Upstream 100 bases:

>100_bases
TGACCACAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTAT
TATTAATGGAGGGGTAAAAC

Downstream 100 bases:

>100_bases
TAACTGATTATCTATAAAGATTCAGTCATTAAAAGCTGTAGCATATGCTACGGCTTTTTTGTTTTAGGTAAAGTAATGTA
AGGAAATTGATTTGAGATAT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex; Membrane-bound ribosome protein complex 50 kDa subunit

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MVVGDFPIETDTIVIGAGPGGYVAAIRAAQLGQKVTIVEKGNLGGVCLNVGCIPSKALLHASHRFVEAQHSENLGVIAES
VSLNFQKVQEFKSSVVNKLTGGVEGLLKGNKVNIVKGEAYFVDNNSLRVMDEKSAQTYNFKNAIIATGSRPIEIPNFKFG
KRVIDSTGALNLQEVPGKLVVVGGGYIGSELGTAFANFGSEVTILEGAKDILGGFEKQMTQPVKKGMKEKGVEIVTEAMA
KSAEETDNGVKVTYEAKGEEKTIEADYVLVTVGRRPNTDELGLEELGVKFADRGLLEVDKQSRTSISNIYAIGDIVPGLP
LAHKASYEAKVAAEAIDGQAAEVDYIGMPAVCFTEPELATVGYSEAQAKEEGLAIKASKFPYAANGRALSLDDTNGFVKL
ITLKEDDTLIGAQVVGTGASDIISELGLAIEAGMNAEDIALTIHAHPTLGEMTMEAAEKAIGYPIHTM

Sequences:

>Translated_468_residues
MVVGDFPIETDTIVIGAGPGGYVAAIRAAQLGQKVTIVEKGNLGGVCLNVGCIPSKALLHASHRFVEAQHSENLGVIAES
VSLNFQKVQEFKSSVVNKLTGGVEGLLKGNKVNIVKGEAYFVDNNSLRVMDEKSAQTYNFKNAIIATGSRPIEIPNFKFG
KRVIDSTGALNLQEVPGKLVVVGGGYIGSELGTAFANFGSEVTILEGAKDILGGFEKQMTQPVKKGMKEKGVEIVTEAMA
KSAEETDNGVKVTYEAKGEEKTIEADYVLVTVGRRPNTDELGLEELGVKFADRGLLEVDKQSRTSISNIYAIGDIVPGLP
LAHKASYEAKVAAEAIDGQAAEVDYIGMPAVCFTEPELATVGYSEAQAKEEGLAIKASKFPYAANGRALSLDDTNGFVKL
ITLKEDDTLIGAQVVGTGASDIISELGLAIEAGMNAEDIALTIHAHPTLGEMTMEAAEKAIGYPIHTM
>Mature_468_residues
MVVGDFPIETDTIVIGAGPGGYVAAIRAAQLGQKVTIVEKGNLGGVCLNVGCIPSKALLHASHRFVEAQHSENLGVIAES
VSLNFQKVQEFKSSVVNKLTGGVEGLLKGNKVNIVKGEAYFVDNNSLRVMDEKSAQTYNFKNAIIATGSRPIEIPNFKFG
KRVIDSTGALNLQEVPGKLVVVGGGYIGSELGTAFANFGSEVTILEGAKDILGGFEKQMTQPVKKGMKEKGVEIVTEAMA
KSAEETDNGVKVTYEAKGEEKTIEADYVLVTVGRRPNTDELGLEELGVKFADRGLLEVDKQSRTSISNIYAIGDIVPGLP
LAHKASYEAKVAAEAIDGQAAEVDYIGMPAVCFTEPELATVGYSEAQAKEEGLAIKASKFPYAANGRALSLDDTNGFVKL
ITLKEDDTLIGAQVVGTGASDIISELGLAIEAGMNAEDIALTIHAHPTLGEMTMEAAEKAIGYPIHTM

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm (Potential). Membrane; Peripheral membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family

Homologues:

Organism=Homo sapiens, GI91199540, Length=461, Percent_Identity=42.9501084598698, Blast_Score=345, Evalue=4e-95,
Organism=Homo sapiens, GI50301238, Length=466, Percent_Identity=27.8969957081545, Blast_Score=181, Evalue=2e-45,
Organism=Homo sapiens, GI22035672, Length=459, Percent_Identity=30.5010893246187, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI33519430, Length=455, Percent_Identity=28.3516483516484, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI33519428, Length=455, Percent_Identity=28.3516483516484, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI33519426, Length=455, Percent_Identity=28.3516483516484, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI291045266, Length=455, Percent_Identity=29.010989010989, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI148277065, Length=455, Percent_Identity=28.3516483516484, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI148277071, Length=455, Percent_Identity=28.3516483516484, Blast_Score=159, Evalue=6e-39,
Organism=Homo sapiens, GI291045268, Length=450, Percent_Identity=26.6666666666667, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1786307, Length=454, Percent_Identity=44.4933920704846, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI87081717, Length=446, Percent_Identity=30.9417040358744, Blast_Score=207, Evalue=8e-55,
Organism=Escherichia coli, GI1789915, Length=433, Percent_Identity=28.8683602771363, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI87082354, Length=457, Percent_Identity=28.4463894967177, Blast_Score=179, Evalue=4e-46,
Organism=Escherichia coli, GI1789065, Length=214, Percent_Identity=28.5046728971963, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI32565766, Length=456, Percent_Identity=42.3245614035088, Blast_Score=344, Evalue=5e-95,
Organism=Caenorhabditis elegans, GI17557007, Length=468, Percent_Identity=29.2735042735043, Blast_Score=159, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI71983429, Length=436, Percent_Identity=27.7522935779816, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI71983419, Length=436, Percent_Identity=27.7522935779816, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI71982272, Length=486, Percent_Identity=26.9547325102881, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17559934, Length=209, Percent_Identity=31.5789473684211, Blast_Score=75, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6321091, Length=472, Percent_Identity=39.4067796610169, Blast_Score=307, Evalue=2e-84,
Organism=Saccharomyces cerevisiae, GI6325166, Length=459, Percent_Identity=28.1045751633987, Blast_Score=179, Evalue=7e-46,
Organism=Saccharomyces cerevisiae, GI6325240, Length=479, Percent_Identity=27.1398747390397, Blast_Score=172, Evalue=8e-44,
Organism=Drosophila melanogaster, GI21358499, Length=468, Percent_Identity=44.2307692307692, Blast_Score=348, Evalue=4e-96,
Organism=Drosophila melanogaster, GI24640549, Length=467, Percent_Identity=29.3361884368308, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI24640553, Length=467, Percent_Identity=29.3361884368308, Blast_Score=155, Evalue=6e-38,
Organism=Drosophila melanogaster, GI24640551, Length=467, Percent_Identity=29.3361884368308, Blast_Score=155, Evalue=8e-38,
Organism=Drosophila melanogaster, GI17737741, Length=469, Percent_Identity=25.7995735607676, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): DLDH_STAAC (Q5HGY8)

Other databases:

- EMBL:   CP000046
- RefSeq:   YP_185969.1
- ProteinModelPortal:   Q5HGY8
- SMR:   Q5HGY8
- STRING:   Q5HGY8
- EnsemblBacteria:   EBSTAT00000009158
- GeneID:   3237345
- GenomeReviews:   CP000046_GR
- KEGG:   sac:SACOL1105
- TIGR:   SACOL1105
- eggNOG:   COG1249
- GeneTree:   EBGT00050000023767
- HOGENOM:   HBG515043
- OMA:   PFIPEDP
- ProtClustDB:   PRK06416
- BioCyc:   SAUR93062:SACOL1105-MONOMER
- GO:   GO:0005737
- GO:   GO:0016020
- GO:   GO:0006096
- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327
- Gene3D:   G3DSA:3.30.390.30
- PANTHER:   PTHR22912:SF20
- PRINTS:   PR00368
- TIGRFAMs:   TIGR01350

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim; SSF55424 FAD/NAD-linked_reductase_dimer

EC number: =1.8.1.4

Molecular weight: Translated: 49452; Mature: 49452

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: ACT_SITE 446-446 BINDING 56-56 BINDING 119-119 BINDING 206-206 BINDING 314-314 BINDING 322-322

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVGDFPIETDTIVIGAGPGGYVAAIRAAQLGQKVTIVEKGNLGGVCLNVGCIPSKALLH
CEECCCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCCCHHHHH
ASHRFVEAQHSENLGVIAESVSLNFQKVQEFKSSVVNKLTGGVEGLLKGNKVNIVKGEAY
HHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEEECCEE
FVDNNSLRVMDEKSAQTYNFKNAIIATGSRPIEIPNFKFGKRVIDSTGALNLQEVPGKLV
EEECCCEEEEECCCCCEEECCCEEEECCCCCEECCCCCHHHHHHCCCCCEEHHHCCCEEE
VVGGGYIGSELGTAFANFGSEVTILEGAKDILGGFEKQMTQPVKKGMKEKGVEIVTEAMA
EEECCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
KSAEETDNGVKVTYEAKGEEKTIEADYVLVTVGRRPNTDELGLEELGVKFADRGLLEVDK
HHHHCCCCCEEEEEECCCCCEEEEEEEEEEEECCCCCCCCCCHHHHCCHHHCCCCEEECC
QSRTSISNIYAIGDIVPGLPLAHKASYEAKVAAEAIDGQAAEVDYIGMPAVCFTEPELAT
HHHCHHHHEEEEHHHCCCCCCCCCCCCCHHHHHHHCCCCCCEEEECCCCEEEECCCCCEE
VGYSEAQAKEEGLAIKASKFPYAANGRALSLDDTNGFVKLITLKEDDTLIGAQVVGTGAS
CCCHHHHHHHCCCEEEECCCCCCCCCCEEEEECCCCEEEEEEEECCCCEEEEEEECCCHH
DIISELGLAIEAGMNAEDIALTIHAHPTLGEMTMEAAEKAIGYPIHTM
HHHHHHCCEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MVVGDFPIETDTIVIGAGPGGYVAAIRAAQLGQKVTIVEKGNLGGVCLNVGCIPSKALLH
CEECCCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCCCHHHHH
ASHRFVEAQHSENLGVIAESVSLNFQKVQEFKSSVVNKLTGGVEGLLKGNKVNIVKGEAY
HHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEEECCEE
FVDNNSLRVMDEKSAQTYNFKNAIIATGSRPIEIPNFKFGKRVIDSTGALNLQEVPGKLV
EEECCCEEEEECCCCCEEECCCEEEECCCCCEECCCCCHHHHHHCCCCCEEHHHCCCEEE
VVGGGYIGSELGTAFANFGSEVTILEGAKDILGGFEKQMTQPVKKGMKEKGVEIVTEAMA
EEECCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
KSAEETDNGVKVTYEAKGEEKTIEADYVLVTVGRRPNTDELGLEELGVKFADRGLLEVDK
HHHHCCCCCEEEEEECCCCCEEEEEEEEEEEECCCCCCCCCCHHHHCCHHHCCCCEEECC
QSRTSISNIYAIGDIVPGLPLAHKASYEAKVAAEAIDGQAAEVDYIGMPAVCFTEPELAT
HHHCHHHHEEEEHHHCCCCCCCCCCCCCHHHHHHHCCCCCCEEEECCCCEEEECCCCCEE
VGYSEAQAKEEGLAIKASKFPYAANGRALSLDDTNGFVKLITLKEDDTLIGAQVVGTGAS
CCCHHHHHHHCCCEEEECCCCCCCCCCEEEEECCCCEEEEEEEECCCCEEEEEEECCCHH
DIISELGLAIEAGMNAEDIALTIHAHPTLGEMTMEAAEKAIGYPIHTM
HHHHHHCCEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA