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The map label for this gene is purH

Identifier: 148267566

GI number: 148267566

Start: 1174733

End: 1176211

Strand: Direct

Name: purH

Synonym: SaurJH9_1133

Alternate gene names: 148267566

Gene position: 1174733-1176211 (Clockwise)

Preceding gene: 148267565

Following gene: 148267567

Centisome position: 40.41

GC content: 34.69

Gene sequence:

>1479_bases
ATGAAGAAAGCTATTTTGAGCGTATCAAATAAAACAGGTATTGTAGAGTTTGCTAAAGCGTTAACGCAATTAAATTATGA
ATTATATTCAACTGGTGGTACTAAACGTATATTAGATGAAGCAAATGTACCAGTTCGTTCTGTTTCAGACTTAACACATT
TTCCAGAAATAATGGATGGCCGTGTTAAAACATTACATCCGGCTGTTCATGGTGGCATTTTAGCTGATCGAAATAAACCG
CAGCATTTAAATGAATTATCAGAACAACATATAGATTTAATTGATATGGTAGTAGTTAATTTATATCCATTCCAACAAAC
TGTTGCAAACCCTGATGTGACGATGGACGAAGCAATTGAAAATATTGATATTGGTGGTCCAACAATGTTACGTGCTGCAG
CTAAAAACTATAAACATGTAACAACAATTGTACATCCGGCAGATTATCATGAAGTATTGACGCGATTAAGAAACGATTCG
TTAGATGAGTCATATAGACAATCATTAATGATTAAAGTTTTTGAGCATACTGCAGAATATGATGAAGCGATTGTACGTTT
CTTTAAAGGGGATAAAGAAACTTTAAGATATGGAGAAAATCCACAACAATCAGCGTATTTTGTGAGAACTTCGAATGCTA
AGCACACGATTGCAGGTGCTAAACAATTACATGGGAAACAATTAAGCTATAACAATATTAAAGATGCAGATGCTACACTA
GCTTTAGTTAAAAAGTTTGATACACCTGCTGCAGTTGCGGTTAAACACATGAATCCATGTGGTGTTGGTATCGGTGACAC
GATTGAACAAGCATTTCAACATGCGTATGAAGCGGATAGTCAATCAATATTTGGTGGAATTGTTGCATTAAACCGAGCTG
TAACACCTGAGTTAGCAGAGCAATTGCATAGTATCTTTTTGGAAGTCATTATTGCACCAAAATTTACAGATGAAGCATTA
GATATTTTAAAACAAAAGAAGAATGTAAGATTATTAGAAATTGATATGACTATAGACAGTAACGAAGAAGAGTTTGTTTC
AGTATCTGGCGGATATTTAGTTCAAGATAAAGACAATTATGTCGTGCCAAAAGAAGAAATGAAAGTTGTTACAGAAGTAG
CACCTACTGATGAACAATGGGAAGCAATGTTATTAGGATGGAAAGTTGTACCATCAGTAAAAAGTAATGCAATTATTTTA
AGTAATAATAAACAAACTGTAGGTATAGGTGCTGGACAAATGAATCGTGTCGGTGCTGCTAAAATTGCGTTAGAGAGAGC
TATTGAAATCAATGATCATGTAGCGTTAGTATCTGATGGATTTTTCCCTATGGGAGATACAGTTGAACTTGCAGCACAAC
ATGGTATAAAGGCAATTATCCAACCGGGTGGTTCGATTAAAGATCAAGATTCAATTGATATGGCTAATAAACATGGTATT
GCAATGGTAGTCACAGGCACTCGACATTTTAAACACTAA

Upstream 100 bases:

>100_bases
ACGATTCAAAAGAACAATTAGAAGAGAAAGTAAAAAAATTGGAATATGAGTTATATCCAAGTGTTATTGCTAAAATTGTA
AAATAAGGAGTATGTATGTG

Downstream 100 bases:

>100_bases
TTTTAAAGGGGCGTAACATAAATGAATGTATTAGTAATTGGTGCTGGTGGACGAGAACATGCACTTGCATATAAACTTAA
TCAATCGAATCTAGTTAAAC

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVPVRSVSDLTHFPEIMDGRVKTLHPAVHGGILADRNKP
QHLNELSEQHIDLIDMVVVNLYPFQQTVANPDVTMDEAIENIDIGGPTMLRAAAKNYKHVTTIVHPADYHEVLTRLRNDS
LDESYRQSLMIKVFEHTAEYDEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIAGAKQLHGKQLSYNNIKDADATL
ALVKKFDTPAAVAVKHMNPCGVGIGDTIEQAFQHAYEADSQSIFGGIVALNRAVTPELAEQLHSIFLEVIIAPKFTDEAL
DILKQKKNVRLLEIDMTIDSNEEEFVSVSGGYLVQDKDNYVVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPSVKSNAIIL
SNNKQTVGIGAGQMNRVGAAKIALERAIEINDHVALVSDGFFPMGDTVELAAQHGIKAIIQPGGSIKDQDSIDMANKHGI
AMVVTGTRHFKH

Sequences:

>Translated_492_residues
MKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVPVRSVSDLTHFPEIMDGRVKTLHPAVHGGILADRNKP
QHLNELSEQHIDLIDMVVVNLYPFQQTVANPDVTMDEAIENIDIGGPTMLRAAAKNYKHVTTIVHPADYHEVLTRLRNDS
LDESYRQSLMIKVFEHTAEYDEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIAGAKQLHGKQLSYNNIKDADATL
ALVKKFDTPAAVAVKHMNPCGVGIGDTIEQAFQHAYEADSQSIFGGIVALNRAVTPELAEQLHSIFLEVIIAPKFTDEAL
DILKQKKNVRLLEIDMTIDSNEEEFVSVSGGYLVQDKDNYVVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPSVKSNAIIL
SNNKQTVGIGAGQMNRVGAAKIALERAIEINDHVALVSDGFFPMGDTVELAAQHGIKAIIQPGGSIKDQDSIDMANKHGI
AMVVTGTRHFKH
>Mature_492_residues
MKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVPVRSVSDLTHFPEIMDGRVKTLHPAVHGGILADRNKP
QHLNELSEQHIDLIDMVVVNLYPFQQTVANPDVTMDEAIENIDIGGPTMLRAAAKNYKHVTTIVHPADYHEVLTRLRNDS
LDESYRQSLMIKVFEHTAEYDEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIAGAKQLHGKQLSYNNIKDADATL
ALVKKFDTPAAVAVKHMNPCGVGIGDTIEQAFQHAYEADSQSIFGGIVALNRAVTPELAEQLHSIFLEVIIAPKFTDEAL
DILKQKKNVRLLEIDMTIDSNEEEFVSVSGGYLVQDKDNYVVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPSVKSNAIIL
SNNKQTVGIGAGQMNRVGAAKIALERAIEINDHVALVSDGFFPMGDTVELAAQHGIKAIIQPGGSIKDQDSIDMANKHGI
AMVVTGTRHFKH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family

Homologues:

Organism=Homo sapiens, GI20127454, Length=461, Percent_Identity=38.8286334056399, Blast_Score=271, Evalue=7e-73,
Organism=Escherichia coli, GI1790439, Length=525, Percent_Identity=47.2380952380952, Blast_Score=452, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI71985564, Length=466, Percent_Identity=34.1201716738197, Blast_Score=238, Evalue=4e-63,
Organism=Caenorhabditis elegans, GI71985574, Length=298, Percent_Identity=27.1812080536913, Blast_Score=82, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6323056, Length=460, Percent_Identity=37.3913043478261, Blast_Score=259, Evalue=5e-70,
Organism=Saccharomyces cerevisiae, GI6323768, Length=469, Percent_Identity=36.4605543710021, Blast_Score=258, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24649832, Length=463, Percent_Identity=37.1490280777538, Blast_Score=257, Evalue=1e-68,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PUR9_STAA2 (A6U0P1)

Other databases:

- EMBL:   CP000736
- RefSeq:   YP_001316296.1
- ProteinModelPortal:   A6U0P1
- SMR:   A6U0P1
- STRING:   A6U0P1
- EnsemblBacteria:   EBSTAT00000016046
- GeneID:   5316421
- GenomeReviews:   CP000736_GR
- KEGG:   sah:SaurJH1_1155
- eggNOG:   COG0138
- GeneTree:   EBGT00050000023867
- HOGENOM:   HBG498048
- OMA:   ASDGFFP
- ProtClustDB:   PRK00881
- BioCyc:   SAUR359787:SAURJH1_1155-MONOMER
- HAMAP:   MF_00139
- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607
- Gene3D:   G3DSA:3.40.50.1380
- PANTHER:   PTHR11692
- PIRSF:   PIRSF000414
- SMART:   SM00798
- SMART:   SM00851
- TIGRFAMs:   TIGR00355

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS; SSF53927 Cytidine_deaminase-like; SSF52335 MGS-like_dom

EC number: =2.1.2.3; =3.5.4.10

Molecular weight: Translated: 54328; Mature: 54328

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVPVRSVSDLTHFPEIMDG
CCCCEEEECCCCCHHHHHHHHHHHCHHEEECCCCHHHHHCCCCCHHHHHHHHHCHHHHCC
RVKTLHPAVHGGILADRNKPQHLNELSEQHIDLIDMVVVNLYPFQQTVANPDVTMDEAIE
CHHEECHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHH
NIDIGGPTMLRAAAKNYKHVTTIVHPADYHEVLTRLRNDSLDESYRQSLMIKVFEHTAEY
HCCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
DEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIAGAKQLHGKQLSYNNIKDADATL
HHHHHHHHCCCHHHHHCCCCCCCCEEEEEECCCCEECCCHHHHCCCCCCCCCCCCHHHHH
ALVKKFDTPAAVAVKHMNPCGVGIGDTIEQAFQHAYEADSQSIFGGIVALNRAVTPELAE
HHHHHCCCCHHHEEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHH
QLHSIFLEVIIAPKFTDEALDILKQKKNVRLLEIDMTIDSNEEEFVSVSGGYLVQDKDNY
HHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCEEEECCCEEEECCCCE
VVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPSVKSNAIILSNNKQTVGIGAGQMNRVGAA
ECCHHHHHHHHHCCCCCHHHHHHEECEEECCCCCCCEEEEECCCEEEEECCCCCCCCHHH
KIALERAIEINDHVALVSDGFFPMGDTVELAAQHGIKAIIQPGGSIKDQDSIDMANKHGI
HHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCE
AMVVTGTRHFKH
EEEEECCCCCCC
>Mature Secondary Structure
MKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVPVRSVSDLTHFPEIMDG
CCCCEEEECCCCCHHHHHHHHHHHCHHEEECCCCHHHHHCCCCCHHHHHHHHHCHHHHCC
RVKTLHPAVHGGILADRNKPQHLNELSEQHIDLIDMVVVNLYPFQQTVANPDVTMDEAIE
CHHEECHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHH
NIDIGGPTMLRAAAKNYKHVTTIVHPADYHEVLTRLRNDSLDESYRQSLMIKVFEHTAEY
HCCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
DEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIAGAKQLHGKQLSYNNIKDADATL
HHHHHHHHCCCHHHHHCCCCCCCCEEEEEECCCCEECCCHHHHCCCCCCCCCCCCHHHHH
ALVKKFDTPAAVAVKHMNPCGVGIGDTIEQAFQHAYEADSQSIFGGIVALNRAVTPELAE
HHHHHCCCCHHHEEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHH
QLHSIFLEVIIAPKFTDEALDILKQKKNVRLLEIDMTIDSNEEEFVSVSGGYLVQDKDNY
HHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCEEEECCCEEEECCCCE
VVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPSVKSNAIILSNNKQTVGIGAGQMNRVGAA
ECCHHHHHHHHHCCCCCHHHHHHEECEEECCCCCCCEEEEECCCEEEEECCCCCCCCHHH
KIALERAIEINDHVALVSDGFFPMGDTVELAAQHGIKAIIQPGGSIKDQDSIDMANKHGI
HHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCE
AMVVTGTRHFKH
EEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA