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The map label for this gene is purD [H]

Identifier: 148267567

GI number: 148267567

Start: 1176233

End: 1177480

Strand: Direct

Name: purD [H]

Synonym: SaurJH9_1134

Alternate gene names: 148267567

Gene position: 1176233-1177480 (Clockwise)

Preceding gene: 148267566

Following gene: 148267573

Centisome position: 40.47

GC content: 35.5

Gene sequence:

>1248_bases
ATGAATGTATTAGTAATTGGTGCTGGTGGACGAGAACATGCACTTGCATATAAACTTAATCAATCGAATCTAGTTAAACA
AGAGTTTGTCATTCCAGGTAATGAGGCAATGACACCTATAGCTGAAGTACACACTGAAATTTCAGAATCTAATCATCAAG
GGATACTAGATTTTGCTAAACAGCAAAATGTTGATTGGGTAGTTATAGGTCCAGAACAGCCGCTAATTGATGGATTAGCA
GACATTTTACGAGCGAATGGTTTCAAAGTGTTTGGTCCAAATAAGCAAGCAGCTCAAATCGAAGGCTCAAAATTATTTGC
TAAAAAGATAATGAAAAAATATAATATTCCAACTGCTGATTATAAAGAAGTTGAGCGAAAAAAGGATGCTTTAACATATA
TTGAAAACTGTGAATTGCCCGTTGTTGTCAAGAAAGATGGGTTAGCTGCTGGGAAAGGCGTTATTATTGCAGATACTATT
GAAGCAGCCAGAAGTGCTATTGAGATTATGTATGGTGATGAAGAAGAAGGTACTGTTGTATTTGAAACGTTTTTAGAAGG
TGAAGAGTTCTCGCTAATGACATTTGTTAATGGTGATTTAGCAGTACCTTTCGACTGTATTGCACAAGATCATAAACGCG
CATTTGATCATGATGAAGGACCAAATACTGGTGGTATGGGGGCTTATTGTCCAGTACCACATATTAGTGACGATGTTTTA
AAACTTACAAATGAAACAATTGCACAACCCATTGCAAAGGCAATGCTTAATGAAGGTTATCAATTCTTCGGTGTATTATA
CATTGGTGCTATTTTAACTAAAGATGGTCCAAAAGTAATAGAATTTAATGCCCGTTTTGGTGATCCTGAAGCTCAAGTAT
TATTAAGTCGCATGGAAAGTGATTTAATGCAGCATATTATTGATTTAGATGAAGGAAAACGTACTGAATTCAAATGGAAA
AATGAATCTATTGTAGGGGTCATGTTGGCATCAAAAGGATATCCTGATGCATATGAAAAAGGGCATAAAGTAAGTGGCTT
TGATTTAAATGAAAACTATTTTGTTAGTGGATTAAAGAAGCAAGGTGATACCTTTGTTACTTCAGGTGGTAGAGTTATAC
TTGCCATCGGAAAAGGTGACAATGTACAAGATGCACAGCGAGACGCATACGAAAAAGTATCTCAAATACAAAGTGACCAT
TTATTCTATCGTCATGACATTGCGAATAAAGCACTACAACTTAAATAA

Upstream 100 bases:

>100_bases
AGATCAAGATTCAATTGATATGGCTAATAAACATGGTATTGCAATGGTAGTCACAGGCACTCGACATTTTAAACACTAAT
TTTAAAGGGGCGTAACATAA

Downstream 100 bases:

>100_bases
GTATATTTAAAAAATACTAAGATTAGCTATGAAGAAATCTATAACGATAGTTTTTTTCATAGCTTTTTTAGTTGTAGAGT
CTAGGACATTGATTTCTGTA

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MNVLVIGAGGREHALAYKLNQSNLVKQEFVIPGNEAMTPIAEVHTEISESNHQGILDFAKQQNVDWVVIGPEQPLIDGLA
DILRANGFKVFGPNKQAAQIEGSKLFAKKIMKKYNIPTADYKEVERKKDALTYIENCELPVVVKKDGLAAGKGVIIADTI
EAARSAIEIMYGDEEEGTVVFETFLEGEEFSLMTFVNGDLAVPFDCIAQDHKRAFDHDEGPNTGGMGAYCPVPHISDDVL
KLTNETIAQPIAKAMLNEGYQFFGVLYIGAILTKDGPKVIEFNARFGDPEAQVLLSRMESDLMQHIIDLDEGKRTEFKWK
NESIVGVMLASKGYPDAYEKGHKVSGFDLNENYFVSGLKKQGDTFVTSGGRVILAIGKGDNVQDAQRDAYEKVSQIQSDH
LFYRHDIANKALQLK

Sequences:

>Translated_415_residues
MNVLVIGAGGREHALAYKLNQSNLVKQEFVIPGNEAMTPIAEVHTEISESNHQGILDFAKQQNVDWVVIGPEQPLIDGLA
DILRANGFKVFGPNKQAAQIEGSKLFAKKIMKKYNIPTADYKEVERKKDALTYIENCELPVVVKKDGLAAGKGVIIADTI
EAARSAIEIMYGDEEEGTVVFETFLEGEEFSLMTFVNGDLAVPFDCIAQDHKRAFDHDEGPNTGGMGAYCPVPHISDDVL
KLTNETIAQPIAKAMLNEGYQFFGVLYIGAILTKDGPKVIEFNARFGDPEAQVLLSRMESDLMQHIIDLDEGKRTEFKWK
NESIVGVMLASKGYPDAYEKGHKVSGFDLNENYFVSGLKKQGDTFVTSGGRVILAIGKGDNVQDAQRDAYEKVSQIQSDH
LFYRHDIANKALQLK
>Mature_415_residues
MNVLVIGAGGREHALAYKLNQSNLVKQEFVIPGNEAMTPIAEVHTEISESNHQGILDFAKQQNVDWVVIGPEQPLIDGLA
DILRANGFKVFGPNKQAAQIEGSKLFAKKIMKKYNIPTADYKEVERKKDALTYIENCELPVVVKKDGLAAGKGVIIADTI
EAARSAIEIMYGDEEEGTVVFETFLEGEEFSLMTFVNGDLAVPFDCIAQDHKRAFDHDEGPNTGGMGAYCPVPHISDDVL
KLTNETIAQPIAKAMLNEGYQFFGVLYIGAILTKDGPKVIEFNARFGDPEAQVLLSRMESDLMQHIIDLDEGKRTEFKWK
NESIVGVMLASKGYPDAYEKGHKVSGFDLNENYFVSGLKKQGDTFVTSGGRVILAIGKGDNVQDAQRDAYEKVSQIQSDH
LFYRHDIANKALQLK

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=426, Percent_Identity=42.7230046948357, Blast_Score=325, Evalue=7e-89,
Organism=Homo sapiens, GI4503915, Length=426, Percent_Identity=42.7230046948357, Blast_Score=323, Evalue=1e-88,
Organism=Homo sapiens, GI209869995, Length=426, Percent_Identity=42.7230046948357, Blast_Score=323, Evalue=1e-88,
Organism=Homo sapiens, GI209869993, Length=426, Percent_Identity=42.7230046948357, Blast_Score=323, Evalue=1e-88,
Organism=Escherichia coli, GI1790438, Length=428, Percent_Identity=43.9252336448598, Blast_Score=336, Evalue=2e-93,
Organism=Caenorhabditis elegans, GI17567511, Length=423, Percent_Identity=38.5342789598109, Blast_Score=248, Evalue=4e-66,
Organism=Saccharomyces cerevisiae, GI6321203, Length=443, Percent_Identity=38.1489841986456, Blast_Score=280, Evalue=3e-76,
Organism=Drosophila melanogaster, GI62471619, Length=432, Percent_Identity=40.2777777777778, Blast_Score=281, Evalue=6e-76,
Organism=Drosophila melanogaster, GI24582400, Length=432, Percent_Identity=40.2777777777778, Blast_Score=280, Evalue=1e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45837; Mature: 45837

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLVIGAGGREHALAYKLNQSNLVKQEFVIPGNEAMTPIAEVHTEISESNHQGILDFAK
CCEEEEECCCCCCEEEEEECCCCCCHHHEECCCCCCCCCHHHHHHHHHCCCCCCHHHHHH
QQNVDWVVIGPEQPLIDGLADILRANGFKVFGPNKQAAQIEGSKLFAKKIMKKYNIPTAD
HCCCCEEEECCCCCHHHHHHHHHHCCCEEEECCCCCCEEECCHHHHHHHHHHHCCCCCCC
YKEVERKKDALTYIENCELPVVVKKDGLAAGKGVIIADTIEAARSAIEIMYGDEEEGTVV
HHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEHHHHHHHHHEEEEECCCCCCEEE
FETFLEGEEFSLMTFVNGDLAVPFDCIAQDHKRAFDHDEGPNTGGMGAYCPVPHISDDVL
EEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHH
KLTNETIAQPIAKAMLNEGYQFFGVLYIGAILTKDGPKVIEFNARFGDPEAQVLLSRMES
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHH
DLMQHIIDLDEGKRTEFKWKNESIVGVMLASKGYPDAYEKGHKVSGFDLNENYFVSGLKK
HHHHHHHCCCCCCCCCEEECCCCEEEEEEECCCCCHHHHCCCCCCCEECCCCCEEECHHC
QGDTFVTSGGRVILAIGKGDNVQDAQRDAYEKVSQIQSDHLFYRHDIANKALQLK
CCCEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCEEEEHHHHCCCEECC
>Mature Secondary Structure
MNVLVIGAGGREHALAYKLNQSNLVKQEFVIPGNEAMTPIAEVHTEISESNHQGILDFAK
CCEEEEECCCCCCEEEEEECCCCCCHHHEECCCCCCCCCHHHHHHHHHCCCCCCHHHHHH
QQNVDWVVIGPEQPLIDGLADILRANGFKVFGPNKQAAQIEGSKLFAKKIMKKYNIPTAD
HCCCCEEEECCCCCHHHHHHHHHHCCCEEEECCCCCCEEECCHHHHHHHHHHHCCCCCCC
YKEVERKKDALTYIENCELPVVVKKDGLAAGKGVIIADTIEAARSAIEIMYGDEEEGTVV
HHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEHHHHHHHHHEEEEECCCCCCEEE
FETFLEGEEFSLMTFVNGDLAVPFDCIAQDHKRAFDHDEGPNTGGMGAYCPVPHISDDVL
EEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHH
KLTNETIAQPIAKAMLNEGYQFFGVLYIGAILTKDGPKVIEFNARFGDPEAQVLLSRMES
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHH
DLMQHIIDLDEGKRTEFKWKNESIVGVMLASKGYPDAYEKGHKVSGFDLNENYFVSGLKK
HHHHHHHCCCCCCCCCEEECCCCEEEEEEECCCCCHHHHCCCCCCCEECCCCCEEECHHC
QGDTFVTSGGRVILAIGKGDNVQDAQRDAYEKVSQIQSDHLFYRHDIANKALQLK
CCCEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCEEEEHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA