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The map label for this gene is carA [H]

Identifier: 147675544

GI number: 147675544

Start: 2110528

End: 2111667

Strand: Reverse

Name: carA [H]

Synonym: VC0395_A1968

Alternate gene names: 147675544

Gene position: 2111667-2110528 (Counterclockwise)

Preceding gene: 147674435

Following gene: 147673654

Centisome position: 69.83

GC content: 50.35

Gene sequence:

>1140_bases
TTGAGTAAATCAGCACTGTTAGTCCTAGAAGATGGGACAGTGTTCCGCGGAGTGTCCATCGGGGCAGATGGTATTTCCGT
CGGTGAAGTCGTTTTCAATACCTCGATGACGGGGTACCAAGAAATCCTCACTGATCCTTCTTATTCCCAGCAAATCGTTA
CTCTTACTTATCCTCACATTGGCAATACCGGAACCAACTCCGAAGACGAAGAATCCACCGCGATTCATGCTCAAGGCCTT
GTGATTCGCGATCTCCCTCTTATCGCTTCTAACTTCCGCAGTGAACAATCCCTCTCTGATTATCTCAAGTCACAAAACAT
TGTCGGTATTGCTGACATTGATACCCGTAAACTGACTCGTATCTTGCGTGAGAAAGGCGCACAGAACGGCTGCATCATGG
CGGGCGATAATCTGGATGAAGCATTAGCACTGGCGAAAGCGAAAGAGTTTCCGGGCCTGAAAGGCATGGATCTCGCGAAA
GTGGTTTCCACCAAAGAAGCGTATGCGTGGAAACAAGGCTCATGGACGCTAGAAGGCGGCCTACCTGAAGCGAAAGCGGA
CAGCGAGCTGCCTTACCATGTTGTGGCTTACGATTTTGGTGCGAAGCGTAACATCCTACGTATGTTGGTGGATCGTGGCT
GCCGCTTAACTGTGGTACCGGCGCAGACCTCTGCAGAAGACGTGCTCGCCCTTAATCCAGACGGTGTGTTTCTATCCAAT
GGCCCAGGCGACCCAGAGCCATGTACTTATGCGATTGAAGCCACACGCGTTTTCCTTGAGAAAAACATTCCTGTTTTTGG
GATCTGTCTAGGTCACCAAATTCTGGCACTCGCCTCCGGCGCGAAAACAGTAAAAATGAAGTTCGGTCACCATGGTGCAA
ACCATCCAGTGAAAGATTTGGATCGCGGTGTGGTGATGATCACCTCGCAAAACCACGGTTTTGCAGCCGATGAAGCGACT
CTGCCTGACAACCTGCGTGCAACCCACAAATCGCTGTTTGATGGTTCACTGCAAGGGATTCATCGTACCGACAAGCCAGC
GTTTAGCTTCCAAGGCCACCCAGAAGCAAGCCCAGGTCCACACGATGCAGCACCGCTGTTTGACCACTTTATTGAACTGA
TTAAACAATTCCGCGCTTAA

Upstream 100 bases:

>100_bases
CAATTTGTCTATAGCATCCTAATGCATGAACTCGTGGCTAGTAGGAAGACGAATTTGCAAAATAATTTATTTTTGATGCA
TTTTTATTCTGGAGGTTATC

Downstream 100 bases:

>100_bases
TTTGGAGTAGTAGATAATGCCAAAACGTACTGACATTCAAAGCATCCTTATCCTTGGTGCGGGTCCAATTGTTATCGGTC
AGGCTTGTGAGTTTGACTAC

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MSKSALLVLEDGTVFRGVSIGADGISVGEVVFNTSMTGYQEILTDPSYSQQIVTLTYPHIGNTGTNSEDEESTAIHAQGL
VIRDLPLIASNFRSEQSLSDYLKSQNIVGIADIDTRKLTRILREKGAQNGCIMAGDNLDEALALAKAKEFPGLKGMDLAK
VVSTKEAYAWKQGSWTLEGGLPEAKADSELPYHVVAYDFGAKRNILRMLVDRGCRLTVVPAQTSAEDVLALNPDGVFLSN
GPGDPEPCTYAIEATRVFLEKNIPVFGICLGHQILALASGAKTVKMKFGHHGANHPVKDLDRGVVMITSQNHGFAADEAT
LPDNLRATHKSLFDGSLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIKQFRA

Sequences:

>Translated_379_residues
MSKSALLVLEDGTVFRGVSIGADGISVGEVVFNTSMTGYQEILTDPSYSQQIVTLTYPHIGNTGTNSEDEESTAIHAQGL
VIRDLPLIASNFRSEQSLSDYLKSQNIVGIADIDTRKLTRILREKGAQNGCIMAGDNLDEALALAKAKEFPGLKGMDLAK
VVSTKEAYAWKQGSWTLEGGLPEAKADSELPYHVVAYDFGAKRNILRMLVDRGCRLTVVPAQTSAEDVLALNPDGVFLSN
GPGDPEPCTYAIEATRVFLEKNIPVFGICLGHQILALASGAKTVKMKFGHHGANHPVKDLDRGVVMITSQNHGFAADEAT
LPDNLRATHKSLFDGSLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIKQFRA
>Mature_378_residues
SKSALLVLEDGTVFRGVSIGADGISVGEVVFNTSMTGYQEILTDPSYSQQIVTLTYPHIGNTGTNSEDEESTAIHAQGLV
IRDLPLIASNFRSEQSLSDYLKSQNIVGIADIDTRKLTRILREKGAQNGCIMAGDNLDEALALAKAKEFPGLKGMDLAKV
VSTKEAYAWKQGSWTLEGGLPEAKADSELPYHVVAYDFGAKRNILRMLVDRGCRLTVVPAQTSAEDVLALNPDGVFLSNG
PGDPEPCTYAIEATRVFLEKNIPVFGICLGHQILALASGAKTVKMKFGHHGANHPVKDLDRGVVMITSQNHGFAADEATL
PDNLRATHKSLFDGSLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIKQFRA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=386, Percent_Identity=39.8963730569948, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI169790915, Length=391, Percent_Identity=36.3171355498721, Blast_Score=221, Evalue=6e-58,
Organism=Homo sapiens, GI21361331, Length=391, Percent_Identity=36.3171355498721, Blast_Score=221, Evalue=6e-58,
Organism=Escherichia coli, GI1786215, Length=378, Percent_Identity=82.5396825396825, Blast_Score=662, Evalue=0.0,
Organism=Caenorhabditis elegans, GI193204318, Length=389, Percent_Identity=37.2750642673522, Blast_Score=234, Evalue=5e-62,
Organism=Saccharomyces cerevisiae, GI6324878, Length=392, Percent_Identity=40.0510204081633, Blast_Score=245, Evalue=9e-66,
Organism=Saccharomyces cerevisiae, GI6322331, Length=397, Percent_Identity=37.27959697733, Blast_Score=229, Evalue=6e-61,
Organism=Drosophila melanogaster, GI45555749, Length=394, Percent_Identity=40.1015228426396, Blast_Score=240, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24642586, Length=394, Percent_Identity=40.1015228426396, Blast_Score=239, Evalue=2e-63,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 40923; Mature: 40792

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSALLVLEDGTVFRGVSIGADGISVGEVVFNTSMTGYQEILTDPSYSQQIVTLTYPHI
CCCCEEEEEECCCEEEEEEECCCCCCHHHEEEECCCCHHHHHHCCCCCCCEEEEEECCCC
GNTGTNSEDEESTAIHAQGLVIRDLPLIASNFRSEQSLSDYLKSQNIVGIADIDTRKLTR
CCCCCCCCCCCCCEEEECCEEEECCHHHHHHCCCHHHHHHHHHCCCEEEEECCCHHHHHH
ILREKGAQNGCIMAGDNLDEALALAKAKEFPGLKGMDLAKVVSTKEAYAWKQGSWTLEGG
HHHHCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHEEECCCEEECCC
LPEAKADSELPYHVVAYDFGAKRNILRMLVDRGCRLTVVPAQTSAEDVLALNPDGVFLSN
CCCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCCEEECC
GPGDPEPCTYAIEATRVFLEKNIPVFGICLGHQILALASGAKTVKMKFGHHGANHPVKDL
CCCCCCCEEEEEHHEEEEECCCCCEEEEEHHHHHHHHHCCCCEEEEEECCCCCCCCHHHH
DRGVVMITSQNHGFAADEATLPDNLRATHKSLFDGSLQGIHRTDKPAFSFQGHPEASPGP
CCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCC
HDAAPLFDHFIELIKQFRA
CCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKSALLVLEDGTVFRGVSIGADGISVGEVVFNTSMTGYQEILTDPSYSQQIVTLTYPHI
CCCEEEEEECCCEEEEEEECCCCCCHHHEEEECCCCHHHHHHCCCCCCCEEEEEECCCC
GNTGTNSEDEESTAIHAQGLVIRDLPLIASNFRSEQSLSDYLKSQNIVGIADIDTRKLTR
CCCCCCCCCCCCCEEEECCEEEECCHHHHHHCCCHHHHHHHHHCCCEEEEECCCHHHHHH
ILREKGAQNGCIMAGDNLDEALALAKAKEFPGLKGMDLAKVVSTKEAYAWKQGSWTLEGG
HHHHCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHEEECCCEEECCC
LPEAKADSELPYHVVAYDFGAKRNILRMLVDRGCRLTVVPAQTSAEDVLALNPDGVFLSN
CCCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCCEEECC
GPGDPEPCTYAIEATRVFLEKNIPVFGICLGHQILALASGAKTVKMKFGHHGANHPVKDL
CCCCCCCEEEEEHHEEEEECCCCCEEEEEHHHHHHHHHCCCCEEEEEECCCCCCCCHHHH
DRGVVMITSQNHGFAADEATLPDNLRATHKSLFDGSLQGIHRTDKPAFSFQGHPEASPGP
CCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCC
HDAAPLFDHFIELIKQFRA
CCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA