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The map label for this gene is aceA [H]

Identifier: 147675086

GI number: 147675086

Start: 281459

End: 282772

Strand: Direct

Name: aceA [H]

Synonym: VC0395_A0267

Alternate gene names: 147675086

Gene position: 281459-282772 (Clockwise)

Preceding gene: 147675709

Following gene: 147675363

Centisome position: 9.31

GC content: 51.45

Gene sequence:

>1314_bases
ATGACACTGACTCGTCGCCAACAAATTGAAGCACTAGAAAAAGATTGGGCAACCAACCCACGCTGGAAAAATGTGAAACG
CACTTACACCGCTGAAGAAGTGGTGAGTTTGCGCGGCTCGGTTGTACCAGCCAATACCATCGCCCAACGTGGTGCAGACA
AACTGTGGGAACTGGTGAATGGTTCAGCGAAAAAAGGGTATGTGAACTGTTTGGGCGCACTCACTGGCGGCCAAGCGGTG
CAACAAGCCAAGGCGGGCATTGAAGCGATTTATCTCTCTGGCTGGCAAGTGGCGGCGGATAACAACACCGCAGGCACCAT
GTACCCAGACCAATCTCTCTATCCAGTGGATTCTGTACCGTCAGTCGTACGCCGCATCAATAATGCGTTTCGTCGCGCTG
ACCAAATCCAGTGGTCAAACGGCAAATCACCACAAGATGAAGGCGGAATTGATTACTTCCTGCCAATCGTCGCGGATGCG
GAAGCCGGTTTTGGTGGCGTACTCAACGCTTATGAGTTGATGAAATCTATGATTGAAGCGGGCGCTGCTGGCGTTCACTT
TGAAGACCAATTGGCTTCGGTGAAAAAATGTGGCCACATGGGCGGCAAAGTATTGGTACCGACTCAAGAAGCGGTGCAAA
AACTGATTGCAGCACGTTTGGCTGCCGATGTTGCAGGCACGACTACGTTAGTGATTGCTCGCACCGATGCTAACGCCGCC
GATTTGCTCACTTCAGACAGCGATCCGTACGATGCCGATTTCGTGACCGGTGAGCGTACTTCCGAAGGTTTCTACCGTGT
ACGCGCGGGCATTGACCAAGCGATTTCTCGCGGCTTGGCTTACGCACCTTACGCAGATTTGGTGTGGTGTGAAACCGCGA
AGCCGGATCTGGAAGAAGCGCGTAAATTTGCTGAAGCGATTCATGCACAATACCCAGATCAACTGTTGGCTTACAACTGC
TCACCGTCGTTCAACTGGGAAAAGAATCTCGATGCGAAAACCATCGCCCATTTCCAGCAAGCGCTGTCGGACATGGGTTA
CAAGTACCAGTTCATCACGTTAGCGGGTATTCACAACATGTGGTTTAACATGTTTGAGTTAGCGCACGCTTACGCGCAAG
GCGAAGGTATGCGTCACTATGTCGAAATGGTACAGCGTCGCGAATTTGAAGCAGCATCCAAAGGTTATACCTTCGTGGCG
CACCAACAAGAAGTGGGGACGGGTTACTTCGATAAGATGACCAACACCATTCAAGGCGGCAACAGTTCGGTGACGGCTCT
GACCGGCTCAACCGAAGAAGATCAATTTCACTAA

Upstream 100 bases:

>100_bases
CACCAGTAACGTGAAACCACTTTACGATCAGCGAGCGCAGTACTGATCGAACAAGGAATCGAGCTGCCAACAGGCAACCA
ATATACAAGAGGGATAGATT

Downstream 100 bases:

>100_bases
TCGATAATCCACCATTCTGTGCGAACCTTGGCGGCCGAATGGCCGCCTTTTTTAGGGTGAGCGTTTACTCTGCTTGAGTA
TGGCGAACCCATTCCTACAT

Product: isocitrate lyase

Products: NA

Alternate protein names: ICL; Isocitrase; Isocitratase [H]

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MTLTRRQQIEALEKDWATNPRWKNVKRTYTAEEVVSLRGSVVPANTIAQRGADKLWELVNGSAKKGYVNCLGALTGGQAV
QQAKAGIEAIYLSGWQVAADNNTAGTMYPDQSLYPVDSVPSVVRRINNAFRRADQIQWSNGKSPQDEGGIDYFLPIVADA
EAGFGGVLNAYELMKSMIEAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLIAARLAADVAGTTTLVIARTDANAA
DLLTSDSDPYDADFVTGERTSEGFYRVRAGIDQAISRGLAYAPYADLVWCETAKPDLEEARKFAEAIHAQYPDQLLAYNC
SPSFNWEKNLDAKTIAHFQQALSDMGYKYQFITLAGIHNMWFNMFELAHAYAQGEGMRHYVEMVQRREFEAASKGYTFVA
HQQEVGTGYFDKMTNTIQGGNSSVTALTGSTEEDQFH

Sequences:

>Translated_437_residues
MTLTRRQQIEALEKDWATNPRWKNVKRTYTAEEVVSLRGSVVPANTIAQRGADKLWELVNGSAKKGYVNCLGALTGGQAV
QQAKAGIEAIYLSGWQVAADNNTAGTMYPDQSLYPVDSVPSVVRRINNAFRRADQIQWSNGKSPQDEGGIDYFLPIVADA
EAGFGGVLNAYELMKSMIEAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLIAARLAADVAGTTTLVIARTDANAA
DLLTSDSDPYDADFVTGERTSEGFYRVRAGIDQAISRGLAYAPYADLVWCETAKPDLEEARKFAEAIHAQYPDQLLAYNC
SPSFNWEKNLDAKTIAHFQQALSDMGYKYQFITLAGIHNMWFNMFELAHAYAQGEGMRHYVEMVQRREFEAASKGYTFVA
HQQEVGTGYFDKMTNTIQGGNSSVTALTGSTEEDQFH
>Mature_436_residues
TLTRRQQIEALEKDWATNPRWKNVKRTYTAEEVVSLRGSVVPANTIAQRGADKLWELVNGSAKKGYVNCLGALTGGQAVQ
QAKAGIEAIYLSGWQVAADNNTAGTMYPDQSLYPVDSVPSVVRRINNAFRRADQIQWSNGKSPQDEGGIDYFLPIVADAE
AGFGGVLNAYELMKSMIEAGAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLIAARLAADVAGTTTLVIARTDANAAD
LLTSDSDPYDADFVTGERTSEGFYRVRAGIDQAISRGLAYAPYADLVWCETAKPDLEEARKFAEAIHAQYPDQLLAYNCS
PSFNWEKNLDAKTIAHFQQALSDMGYKYQFITLAGIHNMWFNMFELAHAYAQGEGMRHYVEMVQRREFEAASKGYTFVAH
QQEVGTGYFDKMTNTIQGGNSSVTALTGSTEEDQFH

Specific function: Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase- positive serine pathway [H]

COG id: COG2224

COG function: function code C; Isocitrate lyase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1790445, Length=433, Percent_Identity=77.8290993071594, Blast_Score=718, Evalue=0.0,
Organism=Escherichia coli, GI1786525, Length=268, Percent_Identity=28.7313432835821, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17561814, Length=420, Percent_Identity=55.952380952381, Blast_Score=469, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI71982926, Length=420, Percent_Identity=55.952380952381, Blast_Score=469, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6320908, Length=290, Percent_Identity=37.9310344827586, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6325263, Length=250, Percent_Identity=38.8, Blast_Score=163, Evalue=5e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006254
- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.1 [H]

Molecular weight: Translated: 47980; Mature: 47849

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTRRQQIEALEKDWATNPRWKNVKRTYTAEEVVSLRGSVVPANTIAQRGADKLWELVN
CCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHC
GSAKKGYVNCLGALTGGQAVQQAKAGIEAIYLSGWQVAADNNTAGTMYPDQSLYPVDSVP
CCCCCCHHHHHHHHCCHHHHHHHHCCCEEEEECCEEEEECCCCCCCCCCCCCCCCCHHHH
SVVRRINNAFRRADQIQWSNGKSPQDEGGIDYFLPIVADAEAGFGGVLNAYELMKSMIEA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHC
GAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLIAARLAADVAGTTTLVIARTDANAA
CCCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHH
DLLTSDSDPYDADFVTGERTSEGFYRVRAGIDQAISRGLAYAPYADLVWCETAKPDLEEA
HHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCHHHH
RKFAEAIHAQYPDQLLAYNCSPSFNWEKNLDAKTIAHFQQALSDMGYKYQFITLAGIHNM
HHHHHHHHHHCCCHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEHHHHHH
WFNMFELAHAYAQGEGMRHYVEMVQRREFEAASKGYTFVAHQQEVGTGYFDKMTNTIQGG
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHCCCHHHHHHHHHCCC
NSSVTALTGSTEEDQFH
CCEEEEEECCCCCCCCC
>Mature Secondary Structure 
TLTRRQQIEALEKDWATNPRWKNVKRTYTAEEVVSLRGSVVPANTIAQRGADKLWELVN
CCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHC
GSAKKGYVNCLGALTGGQAVQQAKAGIEAIYLSGWQVAADNNTAGTMYPDQSLYPVDSVP
CCCCCCHHHHHHHHCCHHHHHHHHCCCEEEEECCEEEEECCCCCCCCCCCCCCCCCHHHH
SVVRRINNAFRRADQIQWSNGKSPQDEGGIDYFLPIVADAEAGFGGVLNAYELMKSMIEA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHC
GAAGVHFEDQLASVKKCGHMGGKVLVPTQEAVQKLIAARLAADVAGTTTLVIARTDANAA
CCCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHH
DLLTSDSDPYDADFVTGERTSEGFYRVRAGIDQAISRGLAYAPYADLVWCETAKPDLEEA
HHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCHHHH
RKFAEAIHAQYPDQLLAYNCSPSFNWEKNLDAKTIAHFQQALSDMGYKYQFITLAGIHNM
HHHHHHHHHHCCCHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEHHHHHH
WFNMFELAHAYAQGEGMRHYVEMVQRREFEAASKGYTFVAHQQEVGTGYFDKMTNTIQGG
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHCCCHHHHHHHHHCCC
NSSVTALTGSTEEDQFH
CCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]