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The map label for this gene is queA

Identifier: 147675363

GI number: 147675363

Start: 283664

End: 284722

Strand: Direct

Name: queA

Synonym: VC0395_A0269

Alternate gene names: 147675363

Gene position: 283664-284722 (Clockwise)

Preceding gene: 147675086

Following gene: 147674126

Centisome position: 9.38

GC content: 50.42

Gene sequence:

>1059_bases
ATGCAAGTTTCAGATTTTCATTTTGAACTCCCAGACGAGCTGATTGCTCGCTACCCAAAAGCAGAACGTACCGCGAGCCG
CTTGCTGCAGCTTGACGGCAACAGTGGTCAACTGGTGGATGGCACCTTTAAAGATGTCCTCGAACTGGTTGAACCGGGAG
ATCTGCTGGTGTTTAACAACACGCGAGTGATCCCAGCTCGCATGTTTGGGCGCAAAGCCAGCGGTGGCAAATTAGAAGTG
TTGGTAGAGCGTATGCTCGATGAGCACACGATTTTAGCGCACGTTCGTTCATCAAAACCGCCCAAACCGGGTACTGAACT
TTATTTAGGCGAGAACGATGAGTTTCATGCCGTGATGCAGGCGCGTCACGATGCGTTGTTTGAAATCCGTTTTACTGCGG
AAACCGTGGTATTGGATATTCTCAATCAAATTGGTCACATGCCGTTGCCACCTTACATCGACCGTCCTGATGAAGAGACA
GATAAAGAGCGTTATCAAACGGTGTACAACCAAAAGCCGGGTGCGGTAGCTGCCCCAACGGCGGGTTTGCATTTTGACCA
AGCGCTGCTTGAGCAGATCCAAGCCAAAGGCGTTGAGTTGGCGTATGTGACACTGCATGTTGGCGCGGGGACCTTCCAGC
CTGTGCGGGTGGAAAATATTCACGATCACCATATGCATGCGGAATACGTTGAAGTGCCACAAGAAGTGGTGGATGCGATT
GCGGCGACCAAAGCGCGTGGTGGTCGAGTGGTTGCTGTGGGTACCACCTCAGTACGTTCACTCGAAAGTGCGGCGCAAGA
TGCGCTGCAAAAAGGCACCGAGTTAAAGCCATTTTTTGGTGATACCGAAATCTTCATCTTCCCGGGGTATCAATACCAGT
TGGTGGATTGCTTGATCACCAATTTCCACTTGCCTGAATCGACCTTGATCATGCTGGTGAGTGCGTTTGCAGGTTATGAG
CACACCATGGCCGCTTATGAACATGCGGTCAAAGAGCAATATCGCTTCTTTAGTTACGGCGATGCGATGTTTATTCGTAA
GCAGACGACTAAAGCGTAA

Upstream 100 bases:

>100_bases
TATCACCACGCAGCATTCCTGTATCTTGAGCGCACTTAGGTATATACTGGCGCGCCGCGAAATCGGGCGACGATACTGAT
CACTTAATCAAGAAAGAGCC

Downstream 100 bases:

>100_bases
TCATCCCTAATCACTTGGAGTGGTTGCTTCAGGTGGCAAGGGATAACGAAAACGAGTGCTGGCAGTAGCCAGAAAAGGTG
CAGACCTTTTTCGCAGGGAA

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MQVSDFHFELPDELIARYPKAERTASRLLQLDGNSGQLVDGTFKDVLELVEPGDLLVFNNTRVIPARMFGRKASGGKLEV
LVERMLDEHTILAHVRSSKPPKPGTELYLGENDEFHAVMQARHDALFEIRFTAETVVLDILNQIGHMPLPPYIDRPDEET
DKERYQTVYNQKPGAVAAPTAGLHFDQALLEQIQAKGVELAYVTLHVGAGTFQPVRVENIHDHHMHAEYVEVPQEVVDAI
AATKARGGRVVAVGTTSVRSLESAAQDALQKGTELKPFFGDTEIFIFPGYQYQLVDCLITNFHLPESTLIMLVSAFAGYE
HTMAAYEHAVKEQYRFFSYGDAMFIRKQTTKA

Sequences:

>Translated_352_residues
MQVSDFHFELPDELIARYPKAERTASRLLQLDGNSGQLVDGTFKDVLELVEPGDLLVFNNTRVIPARMFGRKASGGKLEV
LVERMLDEHTILAHVRSSKPPKPGTELYLGENDEFHAVMQARHDALFEIRFTAETVVLDILNQIGHMPLPPYIDRPDEET
DKERYQTVYNQKPGAVAAPTAGLHFDQALLEQIQAKGVELAYVTLHVGAGTFQPVRVENIHDHHMHAEYVEVPQEVVDAI
AATKARGGRVVAVGTTSVRSLESAAQDALQKGTELKPFFGDTEIFIFPGYQYQLVDCLITNFHLPESTLIMLVSAFAGYE
HTMAAYEHAVKEQYRFFSYGDAMFIRKQTTKA
>Mature_352_residues
MQVSDFHFELPDELIARYPKAERTASRLLQLDGNSGQLVDGTFKDVLELVEPGDLLVFNNTRVIPARMFGRKASGGKLEV
LVERMLDEHTILAHVRSSKPPKPGTELYLGENDEFHAVMQARHDALFEIRFTAETVVLDILNQIGHMPLPPYIDRPDEET
DKERYQTVYNQKPGAVAAPTAGLHFDQALLEQIQAKGVELAYVTLHVGAGTFQPVRVENIHDHHMHAEYVEVPQEVVDAI
AATKARGGRVVAVGTTSVRSLESAAQDALQKGTELKPFFGDTEIFIFPGYQYQLVDCLITNFHLPESTLIMLVSAFAGYE
HTMAAYEHAVKEQYRFFSYGDAMFIRKQTTKA

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=345, Percent_Identity=72.1739130434783, Blast_Score=525, Evalue=1e-150,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_VIBC3 (A5F3H1)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F3H1
- SMR:   A5F3H1
- STRING:   A5F3H1
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0269
- eggNOG:   COG0809
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- ProtClustDB:   PRK00147
- BioCyc:   VCHO345073:VC0395_A0269-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39428; Mature: 39428

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVSDFHFELPDELIARYPKAERTASRLLQLDGNSGQLVDGTFKDVLELVEPGDLLVFNN
CCCCCCCCCCCHHHHHHCCCHHHHHHHHEEECCCCCCEECCHHHHHHHHHCCCCEEEECC
TRVIPARMFGRKASGGKLEVLVERMLDEHTILAHVRSSKPPKPGTELYLGENDEFHAVMQ
CEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHH
ARHDALFEIRFTAETVVLDILNQIGHMPLPPYIDRPDEETDKERYQTVYNQKPGAVAAPT
HHCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEECCC
AGLHFDQALLEQIQAKGVELAYVTLHVGAGTFQPVRVENIHDHHMHAEYVEVPQEVVDAI
CCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHH
AATKARGGRVVAVGTTSVRSLESAAQDALQKGTELKPFFGDTEIFIFPGYQYQLVDCLIT
HHHCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHH
NFHLPESTLIMLVSAFAGYEHTMAAYEHAVKEQYRFFSYGDAMFIRKQTTKA
HCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
>Mature Secondary Structure
MQVSDFHFELPDELIARYPKAERTASRLLQLDGNSGQLVDGTFKDVLELVEPGDLLVFNN
CCCCCCCCCCCHHHHHHCCCHHHHHHHHEEECCCCCCEECCHHHHHHHHHCCCCEEEECC
TRVIPARMFGRKASGGKLEVLVERMLDEHTILAHVRSSKPPKPGTELYLGENDEFHAVMQ
CEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHH
ARHDALFEIRFTAETVVLDILNQIGHMPLPPYIDRPDEETDKERYQTVYNQKPGAVAAPT
HHCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEECCC
AGLHFDQALLEQIQAKGVELAYVTLHVGAGTFQPVRVENIHDHHMHAEYVEVPQEVVDAI
CCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHH
AATKARGGRVVAVGTTSVRSLESAAQDALQKGTELKPFFGDTEIFIFPGYQYQLVDCLIT
HHHCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHH
NFHLPESTLIMLVSAFAGYEHTMAAYEHAVKEQYRFFSYGDAMFIRKQTTKA
HCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA